source: tags/ms_r16q2/lib/pictures/prd_main.fig

#FIG 3.2
Landscape
Center
Metric
A4      
100.00
Single
-2
1200 2
6 1755 1215 8280 1620
2 1 0 2 -1 -1 0 0 -1 0.000 0 0 7 0 0 2
         1785 1575 8235 1575
4 1 -1 0 0 18 12 0.0000 4 180 6465 5010 1380 This module searches for specific PCR-primers in the current sequence. \001
-6
6 720 8280 3105 8460
4 0 0 50 0 16 12 0.0000 4 150 555 2520 8415 $result\001
4 0 0 50 0 16 12 0.0000 4 180 660 720 8415 $design\001
-6
6 2340 4500 5805 5220
4 0 -1 0 0 18 12 0.0000 4 135 840 2340 4635 G+C ratio\001
4 0 -1 0 0 18 12 0.0000 4 180 1095 2340 5085 temperature\001
4 0 0 50 0 16 12 0.0000 4 180 600 3960 4725 $mingc\001
4 0 0 50 0 16 12 0.0000 4 180 780 3960 5175 $mintemp\001
4 0 0 50 0 16 12 0.0000 4 180 645 4950 4725 $maxgc\001
4 0 0 50 0 16 12 0.0000 4 180 825 4950 5175 $maxtemp\001
-6
6 2340 3060 5670 4140
6 4005 3060 5400 3195
4 0 -1 0 0 18 12 0.0000 4 135 360 4005 3195 MIN\001
4 0 -1 0 0 18 12 0.0000 4 135 405 4995 3195 MAX\001
-6
6 2340 3420 5670 4140
4 0 -1 0 0 18 12 0.0000 4 180 1170 2340 3555 primer length\001
4 0 -1 0 0 18 12 0.0000 4 180 1380 2340 4005 primer distance\001
4 0 0 50 0 16 12 0.0000 4 150 675 3960 4095 $mindist\001
4 0 0 50 0 16 12 0.0000 4 150 720 4950 4095 $maxdist\001
4 0 0 50 0 16 12 0.0000 4 150 690 4950 3645 $maxlen\001
4 0 0 50 0 16 12 0.0000 4 150 645 3960 3645 $minlen\001
-6
-6
6 2340 2160 5760 2880
4 0 -1 0 0 18 12 0.0000 4 180 1155 2340 2745 right position\001
4 0 -1 0 0 18 12 0.0000 4 180 1050 2340 2295 left position\001
4 0 0 50 0 16 12 0.0000 4 180 750 3960 2835 $minright\001
4 0 -1 0 0 16 12 0.0000 4 150 630 3960 2385 $minleft\001
4 0 -1 0 0 16 12 0.0000 4 150 675 4950 2385 $maxleft\001
4 0 0 50 0 16 12 0.0000 4 180 795 4950 2835 $maxright\001
-6
6 4005 1800 6840 1935
4 0 -1 0 0 18 12 0.0000 4 135 630 4005 1935 START\001
4 0 -1 0 0 18 12 0.0000 4 135 1845 4995 1935 NUMBER OF BASES\001
-6
6 7020 3060 7470 3600
4 0 0 50 0 16 12 0.0000 4 150 450 7020 3555 $init2\001
4 0 0 50 0 16 12 0.0000 4 150 450 7020 3195 $init1\001
-6
2 2 0 1 -1 -1 0 0 -1 0.000 0 0 0 0 0 5
         9135 8685 9135 600 585 600 585 8685 9135 8685
2 1 0 2 -1 -1 0 0 -1 0.000 0 0 7 0 0 2
         720 7875 9000 7875
2 1 0 1 0 7 50 0 -1 0.000 0 0 7 0 0 2
         720 4260 9000 4260
2 1 0 1 0 7 50 0 -1 0.000 0 0 7 0 0 2
         720 5340 9000 5340
2 2 0 1 -1 -1 0 0 -1 0.000 0 0 0 0 0 5
         8865 3735 8865 2025 6345 2025 6345 3735 8865 3735
4 0 0 50 0 16 12 0.0000 4 150 555 720 1035 $close\001
4 2 0 50 0 16 12 0.0000 4 180 465 9000 1035 $help\001
4 2 0 50 0 16 10 0.0000 4 150 2355 9045 8595 written by Wolfram Foerster 2001\001
4 0 0 50 0 18 12 0.0000 4 180 870 6345 4005 0 = ignore\001
4 2 -1 0 0 18 12 0.0000 4 180 2940 3780 5715 min. dist to next match of primer\001
4 2 -1 0 0 18 12 0.0000 4 180 1830 3780 6165 expand IUPAC codes\001
4 2 -1 0 0 18 12 0.0000 4 135 2070 3780 6615 max. number of results\001
4 2 -1 0 0 18 12 0.0000 4 135 1110 3780 7065 G+C - factor\001
4 2 -1 0 0 18 12 0.0000 4 180 1695 3780 7515 temperature factor\001
4 0 -1 0 0 18 12 0.0000 4 180 1860 5130 5715 <= 0 : singular match\001
4 0 0 50 0 16 12 0.0000 4 180 1065 3960 7605 $temp_factor\001
4 0 0 50 0 16 12 0.0000 4 165 945 3960 7155 $GC_factor\001
4 0 0 50 0 16 12 0.0000 4 180 945 3960 6705 $max_pairs\001
4 0 0 50 0 16 12 0.0000 4 180 1380 3960 6255 $expand_IUPAC\001
4 0 0 50 0 16 12 0.0000 4 165 1350 3960 5805 $allowed_match\001
4 0 0 50 0 16 35 0.0000 4 210 75 5850 7380 }\001
4 0 -1 0 0 18 12 0.0000 4 135 660 5130 7515 0 .. 100\001
4 0 -1 0 0 18 12 0.0000 4 135 660 5130 7065 0 .. 100\001
4 0 0 50 0 18 12 0.0000 4 180 1830 6030 7290 G+C  +  temp  =  100\001
4 0 0 50 0 16 12 0.0000 4 180 1050 7425 8280 $aprox_mem\001
4 0 0 50 0 18 12 0.0000 4 180 2490 4815 8190 estimated memory needed :\001
4 1 0 50 0 18 12 0.0000 4 135 2055 7605 2565 with reasonable values\001
4 1 0 50 0 18 12 0.0000 4 180 2250 7605 2340 fill positions and distance\001
4 1 0 50 0 18 12 0.0000 4 135 1650 7605 2790 from the current ..\001
4 0 0 50 0 0 12 0.0000 4 195 555 3780 8325 $config\001