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| 2 | Welcome to the 'ARB' Sequence Database Tools |
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| 3 | |
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| 4 | /*********** Hardware and System Requirements *************/ |
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| 5 | |
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| 6 | ARB is currently developed on Linux PCs only. |
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| 7 | |
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| 8 | Compiling ARB using the gcc compiler (versions 2.95.3 and 3.x series) |
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| 9 | as well works on Mac OSX and Sun OS - but we do not publish or support |
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| 10 | these versions. |
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| 11 | |
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| 12 | |
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| 13 | Hardware Requirements (Linux): |
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| 14 | |
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| 15 | Good We use |
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| 16 | Real memory : 256 Mb 1 Gb |
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| 17 | Free discspace : 1 Gb more |
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| 18 | Computer speed : Pentium III; 400 Mhz Dual Pentium III; 650 Mhz |
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| 19 | Ultra Sparc Ultra Sparc |
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| 20 | |
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| 21 | Note : Memory is more important than a fast processor; a 486dx width |
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| 22 | 64 mByte of RAM may be much faster than an Ultra Sparc with |
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| 23 | 32 mByte of RAM. |
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| 24 | |
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| 25 | |
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| 26 | /*********** Files needed to install ARB *************/ |
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| 27 | |
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| 28 | File FTP server location // Comment |
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| 29 | |
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| 30 | 'arb_README.txt' pub/ARB/ // this file |
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| 31 | 'arb_install.sh' pub/ARB/$MACH // install script |
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| 32 | 'arb.tgz' pub/ARB/$MACH // ARB program |
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| 33 | 'zcat' pub/ARB/$MACH // decompress (gzip) |
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| 34 | ['arb_ale.tgz' pub/ARB/$MACH // optional Editor ] |
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| 35 | ['***.arb' pub/ARB/data/ // optional demo / |
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| 36 | real rRNA data ] |
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| 37 | |
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| 38 | Notes: |
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| 39 | - $MACH should be replaced by your system type |
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| 40 | ( type uname -sr to find out your system type ) |
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| 41 | - enable binary mode for ftp transfer ( command 'bin' ) |
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| 42 | - do not uncompress and untar arb.tar.gz directly, use the |
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| 43 | install script !!! |
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| 44 | |
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| 45 | /*********** Install/Update ARB *************/ |
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| 46 | |
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| 47 | ARB consists of more than 750 files which are installed into a single |
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| 48 | directory. Creating this directory, copying all data into it, and setting |
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| 49 | the permissions correctly is done by the installation script |
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| 50 | |
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| 51 | 'arb_install' |
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| 52 | |
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| 53 | Goto the directory, where the files |
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| 54 | |
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| 55 | 'arb_install.sh' //install script |
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| 56 | 'arb_README.txt' //this file |
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| 57 | 'arb.tgz' //all the libs and bin |
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| 58 | 'zcat' //decompress |
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| 59 | [ 'arb_ale.tgz' //optional sequence editor ] |
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| 60 | |
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| 61 | are located and type '/bin/sh arb_install.sh' |
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| 62 | |
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| 63 | Answer all questions asked by the script. |
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| 64 | Notes: -The script will ask about a pt_server directory. This is a |
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| 65 | directory where arb will store big index files. |
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| 66 | You should enter a different path as you do not want to |
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| 67 | recreate those files after an ARB update. |
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| 68 | - Normally pressing enter will be a good choice. |
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| 69 | - You can rerun the script many times, it can be used to |
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| 70 | change an existing installation. |
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| 71 | |
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| 72 | Change your .cshrc/.profile files: |
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| 73 | |
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| 74 | Set the enviroment variable ARBHOME |
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| 75 | to the ARB installation directory |
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| 76 | Append $ARBHOME/bin to your PATH |
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| 77 | |
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| 78 | reread it, (logout+login ) |
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| 79 | |
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| 80 | goto a directory with a demo database 'eg demo.arb' |
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| 81 | and start 'ARB' with |
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| 82 | |
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| 83 | 'arb' |
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| 84 | |
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| 85 | /*********** Additional information *************/ |
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| 86 | |
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| 87 | * See arb_INSTALL.txt for additional software needed and/or useful |
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| 88 | together with ARB. |
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| 89 | |
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| 90 | * |
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| 91 | |
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| 92 | |
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| 93 | /*********** PT_server *************/ |
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| 94 | |
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| 95 | To Install 'ARB' you have to know that some modules use a so |
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| 96 | called 'pt_server' (prefix tree server). |
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| 97 | |
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| 98 | ARB needs a writeable directory to store the pt_server files. |
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| 99 | Those files are needed for fast database search |
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| 100 | (by probe_design, probe_match and the automatic aligner) |
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| 101 | and need a lot of disc space (up to several 100 mega bytes, |
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| 102 | e.g. 4000 16S RNA sequences require about 40 MB). |
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| 103 | This files are not created now. They can be build by any user via |
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| 104 | <ARB_NTREE/Probes/PT_SERVER admin/Build server> |
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| 105 | You may define a special directory as the pt_server files location. |
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| 106 | This prevents any loss of data installing a new version of |
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| 107 | ARB. |
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| 108 | To minimize the use of ressources in a workstation cluster |
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| 109 | only one pt_server for each database is started on a user defined |
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| 110 | host. The first user starts the pt_server, and all other users |
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| 111 | can connect to it. |
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| 112 | |
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| 113 | /*********** What you should know: Swap *************/ |
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| 114 | |
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| 115 | Arb needs a lot of virtual memory |
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| 116 | (about 50 Mbyte for 5000 Sequences, Length = 3000). |
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| 117 | You can find out about installed swap space by typing: |
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| 118 | |
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| 119 | SunOS 1.x: 'pstat -s' |
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| 120 | System V / SGI 'swap -s' |
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| 121 | OSF 'swapon -sv' |
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| 122 | Linux 'top' |
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| 123 | |
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| 124 | Call your system administrator or local guru to increase your |
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| 125 | swap. (If you don't have such a nice person, try to read the |
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| 126 | man pages: 'man -k swap') |
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| 127 | |
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| 128 | |
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| 129 | /************* The Database ************/ |
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| 130 | |
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| 131 | In the current release a small dataset (demo.arb) is provided. |
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| 132 | This database contains a selection of artificial and real-life |
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| 133 | sequences. |
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| 134 | |
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| 135 | The intention of providing this small dataset first is to give |
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| 136 | you the opportunity to get familiar with the program and to |
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| 137 | test the performance of your computer system as well as the |
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| 138 | stability of ARB on your system. |
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| 139 | |
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| 140 | ARB is running properly and stably on our systems. However, it |
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| 141 | may be that there are bugs never detected by us or never |
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| 142 | appearing on our systems. Please don't hesitate to inform us |
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| 143 | about any bugs. A detailed description of the steps performed |
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| 144 | before the problem was evident and of the number and types of |
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| 145 | modules running at the same time would extremely be helpful to |
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| 146 | our computer scientists. |
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| 147 | |
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| 148 | |
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| 149 | /*********** Support *************/ |
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| 150 | |
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| 151 | Please send any comments, bug reports or questions to |
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| 152 | |
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| 153 | arb@arb-home.de |
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| 154 | |
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| 155 | |
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| 156 | /*********** Copyright Notice *************/ |
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| 157 | |
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| 158 | Please see the file |
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| 159 | |
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| 160 | arb_LICENSE.txt |
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| 161 | |
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| 162 | in the ARB installation directory. |
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| 163 | |
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| 164 | /*********** Disclaimer *************/ |
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| 165 | |
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| 166 | THE AUTHORS OF ARB GIVE NO WARRANTIES, EXPRESSED OR IMPLIED |
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| 167 | FOR THE SOFTWARE AND DOCUMENTATION PROVIDED, INCLUDING, BUT |
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| 168 | NOT LIMITED TO WARRANTY OF MERCHANTABILITY AND WARRANTY OF |
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| 169 | FITNESS FOR A PARTICULAR PURPOSE. |
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