| 1 | __VERSION__="ete2-2.2rev1026" |
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| 2 | # -*- coding: utf-8 -*- |
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| 3 | # #START_LICENSE########################################################### |
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| 4 | # |
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| 5 | # |
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| 6 | # This file is part of the Environment for Tree Exploration program |
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| 7 | # (ETE). http://ete.cgenomics.org |
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| 8 | # |
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| 9 | # ETE is free software: you can redistribute it and/or modify it |
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| 10 | # under the terms of the GNU General Public License as published by |
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| 11 | # the Free Software Foundation, either version 3 of the License, or |
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| 12 | # (at your option) any later version. |
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| 13 | # |
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| 14 | # ETE is distributed in the hope that it will be useful, but WITHOUT |
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| 15 | # ANY WARRANTY; without even the implied warranty of MERCHANTABILITY |
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| 16 | # or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public |
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| 17 | # License for more details. |
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| 18 | # |
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| 19 | # You should have received a copy of the GNU General Public License |
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| 20 | # along with ETE. If not, see <http://www.gnu.org/licenses/>. |
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| 21 | # |
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| 22 | # |
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| 23 | # ABOUT THE ETE PACKAGE |
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| 24 | # ===================== |
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| 25 | # |
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| 26 | # ETE is distributed under the GPL copyleft license (2008-2011). |
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| 27 | # |
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| 28 | # If you make use of ETE in published work, please cite: |
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| 29 | # |
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| 30 | # Jaime Huerta-Cepas, Joaquin Dopazo and Toni Gabaldon. |
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| 31 | # ETE: a python Environment for Tree Exploration. Jaime BMC |
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| 32 | # Bioinformatics 2010,:24doi:10.1186/1471-2105-11-24 |
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| 33 | # |
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| 34 | # Note that extra references to the specific methods implemented in |
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| 35 | # the toolkit are available in the documentation. |
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| 36 | # |
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| 37 | # More info at http://ete.cgenomics.org |
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| 38 | # |
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| 39 | # |
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| 40 | # #END_LICENSE############################################################# |
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| 41 | |
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| 42 | import os |
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| 43 | import string |
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| 44 | import textwrap |
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| 45 | from sys import stderr as STDERR |
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| 46 | |
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| 47 | def read_fasta(source, obj=None, header_delimiter="\t", fix_duplicates=True): |
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| 48 | """ Reads a collection of sequences econded in FASTA format.""" |
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| 49 | |
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| 50 | if obj is None: |
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| 51 | from ete2.coretype import seqgroup |
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| 52 | SC = seqgroup.SeqGroup() |
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| 53 | else: |
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| 54 | SC = obj |
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| 55 | |
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| 56 | names = set([]) |
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| 57 | seq_id = -1 |
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| 58 | |
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| 59 | # Prepares handle from which read sequences |
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| 60 | if os.path.isfile(source): |
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| 61 | _source = open(source, "rU") |
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| 62 | else: |
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| 63 | _source = iter(source.split("\n")) |
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| 64 | |
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| 65 | seq_name = None |
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| 66 | for line in _source: |
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| 67 | line = line.strip() |
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| 68 | if line.startswith('#') or not line: |
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| 69 | continue |
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| 70 | # Reads seq number |
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| 71 | elif line.startswith('>'): |
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| 72 | # Checks if previous name had seq |
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| 73 | if seq_id>-1 and SC.id2seq[seq_id] == "": |
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| 74 | raise Exception, "No sequence found for "+seq_name |
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| 75 | |
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| 76 | seq_id += 1 |
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| 77 | # Takes header info |
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| 78 | seq_header_fields = map(string.strip, line[1:].split(header_delimiter)) |
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| 79 | seq_name = seq_header_fields[0] |
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| 80 | |
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| 81 | # Checks for duplicated seq names |
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| 82 | if fix_duplicates and seq_name in names: |
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| 83 | tag = str(len([k for k in SC.name2id.keys() if k.endswith(seq_name)])) |
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| 84 | old_name = seq_name |
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| 85 | seq_name = tag+"_"+seq_name |
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| 86 | print >>STDERR, "Duplicated entry [%s] was renamed to [%s]" %(old_name, seq_name) |
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| 87 | |
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| 88 | # stores seq_name |
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| 89 | SC.id2seq[seq_id] = "" |
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| 90 | SC.id2name[seq_id] = seq_name |
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| 91 | SC.name2id[seq_name] = seq_id |
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| 92 | SC.id2comment[seq_id] = seq_header_fields[1:] |
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| 93 | names.add(seq_name) |
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| 94 | |
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| 95 | else: |
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| 96 | if seq_name is None: |
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| 97 | raise Exception, "Error reading sequences: Wrong format." |
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| 98 | |
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| 99 | # removes all white spaces in line |
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| 100 | s = line.strip().replace(" ","") |
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| 101 | |
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| 102 | # append to seq_string |
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| 103 | SC.id2seq[seq_id] += s |
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| 104 | |
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| 105 | if seq_name and SC.id2seq[seq_id] == "": |
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| 106 | print >>STDERR, seq_name,"has no sequence" |
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| 107 | return None |
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| 108 | |
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| 109 | # Everything ok |
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| 110 | return SC |
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| 111 | |
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| 112 | def write_fasta(sequences, outfile = None, seqwidth = 80): |
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| 113 | """ Writes a SeqGroup python object using FASTA format. """ |
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| 114 | |
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| 115 | wrapper = textwrap.TextWrapper() |
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| 116 | wrapper.break_on_hyphens = False |
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| 117 | wrapper.replace_whitespace = False |
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| 118 | wrapper.expand_tabs = False |
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| 119 | wrapper.break_long_words = True |
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| 120 | wrapper.width = 80 |
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| 121 | text = '\n'.join([">%s\n%s\n" %( "\t".join([name]+comment), wrapper.fill(seq)) for |
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| 122 | name, seq, comment, sid in sequences]) |
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| 123 | |
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| 124 | if outfile is not None: |
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| 125 | OUT = open(outfile,"w") |
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| 126 | OUT.write(text) |
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| 127 | OUT.close() |
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| 128 | else: |
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| 129 | return text |
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| 130 | |
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| 131 | def write_fasta_internal(sequences, outfile = None, seqwidth = 80): |
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| 132 | """ Writes a SeqGroup python object using FASTA format. """ |
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| 133 | |
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| 134 | wrapper = textwrap.TextWrapper() |
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| 135 | wrapper.break_on_hyphens = False |
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| 136 | wrapper.replace_whitespace = False |
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| 137 | wrapper.expand_tabs = False |
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| 138 | wrapper.break_long_words = True |
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| 139 | wrapper.width = 80 |
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| 140 | text = '\n'.join([">%s\n%s\n" %( "\t".join([str(sid)]+comment), wrapper.fill(seq)) for |
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| 141 | name, seq, comment, sid in sequences]) |
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| 142 | |
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| 143 | if outfile is not None: |
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| 144 | OUT = open(outfile,"w") |
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| 145 | OUT.write(text) |
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| 146 | OUT.close() |
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| 147 | else: |
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| 148 | return text |
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