1 | // =============================================================== // |
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2 | // // |
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3 | // File : graph_aligner_gui.cxx // |
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4 | // Purpose : // |
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5 | // // |
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6 | // Coded by Elmar Pruesse in October 2008 // |
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7 | // Institute of Microbiology (Technical University Munich) // |
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8 | // http://www.arb-home.de/ // |
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9 | // // |
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10 | // =============================================================== // |
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11 | |
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12 | #include "graph_aligner_gui.hxx" |
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13 | |
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14 | #include "ed4_defs.hxx" |
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15 | |
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16 | // need to use AlignDataAccess defined here to get selected species |
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17 | #include <fast_aligner.hxx> |
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18 | |
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19 | #include <aw_awars.hxx> |
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20 | #include <aw_msg.hxx> |
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21 | #include <aw_root.hxx> |
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22 | #include <awt_sel_boxes.hxx> |
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23 | #include <awt_config_manager.hxx> |
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24 | #include <servercntrl.h> |
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25 | #include <PT_com.h> |
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26 | #include <client.h> |
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27 | #include <arbdbt.h> |
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28 | #include <arb_strbuf.h> |
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29 | #include <arb_misc.h> |
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30 | |
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31 | #include <sys/types.h> |
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32 | #include <sys/wait.h> |
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33 | |
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34 | #include <unistd.h> |
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35 | |
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36 | #include <string> |
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37 | #include <sstream> |
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38 | #include <iostream> |
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39 | #include <vector> |
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40 | |
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41 | using std::cerr; |
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42 | using std::cout; |
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43 | using std::endl; |
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44 | |
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45 | #define GA_AWAR_ROOT "sina/" |
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46 | |
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47 | #define GA_AWAR_CMD GA_AWAR_ROOT "command" |
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48 | #define GA_AWAR_TGT GA_AWAR_ROOT "target" |
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49 | #define GA_AWAR_SAI GA_AWAR_ROOT "sai" |
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50 | #define GA_AWAR_ALIGNMENT GA_AWAR_ROOT "alignment" |
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51 | #define GA_AWAR_PROTECTION GA_AWAR_ROOT "protection" |
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52 | #define GA_AWAR_TURN_CHECK GA_AWAR_ROOT "turncheck" |
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53 | #define GA_AWAR_REALIGN GA_AWAR_ROOT "realign" |
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54 | #define GA_AWAR_COPYMARKREF GA_AWAR_ROOT "copymarkref" |
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55 | #define GA_AWAR_MATCH_SCORE GA_AWAR_ROOT "match_score" |
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56 | #define GA_AWAR_MISMATCH_SCORE GA_AWAR_ROOT "mismatch_score" |
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57 | #define GA_AWAR_GAP_PEN GA_AWAR_ROOT "gap_pen" |
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58 | #define GA_AWAR_GAP_EXT GA_AWAR_ROOT "gap_ext" |
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59 | #define GA_AWAR_ADVANCED GA_AWAR_ROOT "advanced" |
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60 | #define GA_AWAR_FS_MIN GA_AWAR_ROOT "fs_min" |
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61 | #define GA_AWAR_FS_MAX GA_AWAR_ROOT "fs_max" |
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62 | #define GA_AWAR_FS_MSC GA_AWAR_ROOT "fs_msc" |
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63 | #define GA_AWAR_MIN_FULL GA_AWAR_ROOT "min_full" |
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64 | #define GA_AWAR_FULL_MINLEN GA_AWAR_ROOT "full_minlen" |
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65 | #define GA_AWAR_OVERHANG GA_AWAR_ROOT "overhang" |
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66 | #define GA_AWAR_THREADS GA_AWAR_ROOT "threads" |
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67 | #define GA_AWAR_QSIZE GA_AWAR_ROOT "qsize" |
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68 | #define GA_AWAR_KMER_LEN GA_AWAR_ROOT "kmer_len" |
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69 | #define GA_AWAR_KMER_MM GA_AWAR_ROOT "kmer_mm" |
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70 | #define GA_AWAR_MIN_LEN GA_AWAR_ROOT "min_len" |
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71 | #define GA_AWAR_WEIGHT GA_AWAR_ROOT "weight" |
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72 | #define GA_AWAR_INSERT GA_AWAR_ROOT "insert" |
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73 | #define GA_AWAR_LOWERCASE GA_AWAR_ROOT "lowercase" |
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74 | #define GA_AWAR_AUTOFILTER GA_AWAR_ROOT "autofilter" |
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75 | #define GA_AWAR_KMER_NOREL GA_AWAR_ROOT "kmer_norel" |
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76 | #define GA_AWAR_KMER_NOFAST GA_AWAR_ROOT "kmer_nofast" |
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77 | #define GA_AWAR_SHOW_DIST GA_AWAR_ROOT "show_dist" |
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78 | #define GA_AWAR_SHOW_DIFF GA_AWAR_ROOT "show_diff" |
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79 | #define GA_AWAR_COLOR GA_AWAR_ROOT "color" |
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80 | #define GA_AWAR_GENE_START GA_AWAR_ROOT "gene_start" |
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81 | #define GA_AWAR_GENE_END GA_AWAR_ROOT "gene_end" |
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82 | #define GA_AWAR_FS_COVER_GENE GA_AWAR_ROOT "fs_cover_gene" |
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83 | |
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84 | void create_sina_variables(AW_root *root, AW_default db1) { |
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85 | root->awar_string(GA_AWAR_CMD, "sina", db1); |
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86 | root->awar_int (GA_AWAR_TGT, 2, db1); |
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87 | root->awar_int (AWAR_PT_SERVER, 0, db1); |
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88 | root->awar_string(GA_AWAR_SAI, "", db1); |
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89 | root->awar_int (GA_AWAR_PROTECTION, 0, db1); |
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90 | root->awar_int (GA_AWAR_TURN_CHECK, 1, db1); |
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91 | root->awar_int (GA_AWAR_REALIGN, 1, db1); |
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92 | root->awar_int (GA_AWAR_COPYMARKREF, 0, db1); |
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93 | root->awar_float (GA_AWAR_GAP_PEN, 5.0, db1); |
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94 | root->awar_float (GA_AWAR_GAP_EXT, 2.0, db1); |
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95 | root->awar_float (GA_AWAR_MATCH_SCORE, 2.0, db1); |
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96 | root->awar_float (GA_AWAR_MISMATCH_SCORE, -1.0, db1); |
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97 | root->awar_int (GA_AWAR_ADVANCED, 0, db1); |
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98 | root->awar_int (GA_AWAR_FS_MIN, 40, db1); |
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99 | root->awar_int (GA_AWAR_FS_MAX, 40, db1); |
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100 | root->awar_float (GA_AWAR_FS_MSC, .7, db1); |
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101 | root->awar_int (GA_AWAR_MIN_FULL, 1, db1); |
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102 | root->awar_int (GA_AWAR_FULL_MINLEN, 1400, db1); |
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103 | root->awar_string(GA_AWAR_OVERHANG, "attach", db1); |
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104 | root->awar_int (GA_AWAR_THREADS, 1, db1); |
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105 | root->awar_int (GA_AWAR_QSIZE, 1, db1); |
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106 | root->awar_int (GA_AWAR_KMER_LEN, 10, db1); |
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107 | root->awar_int (GA_AWAR_KMER_MM, 0, db1); |
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108 | root->awar_int (GA_AWAR_MIN_LEN, 150, db1); |
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109 | root->awar_float (GA_AWAR_WEIGHT, 1, db1); |
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110 | root->awar_string(GA_AWAR_INSERT, "shift", db1); |
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111 | root->awar_string(GA_AWAR_LOWERCASE, "none", db1); |
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112 | root->awar_string(GA_AWAR_AUTOFILTER, "none", db1); |
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113 | root->awar_int (GA_AWAR_KMER_NOREL, 0, db1); |
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114 | root->awar_int (GA_AWAR_KMER_NOFAST, 0, db1); |
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115 | root->awar_int (GA_AWAR_SHOW_DIST, 0, db1); |
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116 | root->awar_int (GA_AWAR_SHOW_DIFF, 0, db1); |
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117 | root->awar_int (GA_AWAR_COLOR, 1, db1); |
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118 | root->awar_int (GA_AWAR_GENE_START, 0, db1); |
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119 | root->awar_int (GA_AWAR_GENE_END, 0, db1); |
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120 | root->awar_int (GA_AWAR_FS_COVER_GENE, 1, db1); |
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121 | } |
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122 | |
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123 | AW_active sina_mask(AW_root *root) { |
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124 | const char *sinaName = root->awar(GA_AWAR_CMD)->read_char_pntr(); |
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125 | char *sina = ARB_executable(sinaName, GB_getenv("PATH")); |
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126 | const char *fail_reason = NULp; |
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127 | |
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128 | if (sina) { |
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129 | int exitstatus = system(GBS_global_string("%s --has-cli-vers ARB5.99", sina)); |
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130 | exitstatus = WEXITSTATUS(exitstatus); |
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131 | |
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132 | switch (exitstatus) { |
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133 | case EXIT_SUCCESS: |
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134 | break; |
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135 | |
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136 | case EXIT_FAILURE: |
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137 | fail_reason = "Incompatible SINA and ARB versions"; |
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138 | break; |
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139 | |
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140 | case 127: // libs missing |
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141 | default: // unexpected |
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142 | fail_reason = GBS_global_string("Could execute SINA binary '%s' (exitstatus was %i)", |
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143 | sinaName, exitstatus); |
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144 | break; |
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145 | } |
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146 | free(sina); |
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147 | } |
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148 | else { |
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149 | fail_reason = GBS_global_string("%s not found", sinaName); |
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150 | } |
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151 | |
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152 | if (fail_reason) { |
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153 | fprintf(stderr, |
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154 | "Note: SINA (SILVA Aligner) disabled (Reason: %s)\n", |
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155 | fail_reason); |
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156 | } |
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157 | |
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158 | return fail_reason ? AWM_DISABLED : AWM_ALL; |
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159 | } |
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160 | |
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161 | inline const char *stream2static(const std::stringstream& str) { |
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162 | return GBS_static_string(str.str().c_str()); |
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163 | } |
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164 | |
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165 | inline const char *empty_as_none(const char *sainame) { |
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166 | // see http://bugs.arb-home.de/ticket/519 |
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167 | if (sainame && !sainame[0]) sainame = "none"; |
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168 | return sainame; |
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169 | } |
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170 | |
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171 | static void sina_start(AW_window *window, const AlignDataAccess *data_access) { |
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172 | cerr << "Starting SINA..." << endl; |
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173 | |
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174 | // make string from pt server selection |
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175 | AW_root *root = window->get_root(); |
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176 | int pt = root->awar(AWAR_PT_SERVER)->read_int(); |
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177 | |
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178 | std::stringstream ptnam; |
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179 | ptnam << "ARB_PT_SERVER" << pt; |
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180 | |
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181 | const char *pt_server = GBS_read_arb_tcp(ptnam.str().c_str()); |
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182 | GB_ERROR gb_error = NULp; |
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183 | |
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184 | if (!pt_server) { |
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185 | gb_error = GBS_global_string("Unable to find definition for chosen PT-server\n(Reason: %s)", GB_await_error()); |
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186 | } |
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187 | else { |
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188 | const char *pt_db = pt_server + strlen(pt_server) + 1; |
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189 | pt_db += strlen(pt_db)+3; |
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190 | |
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191 | // start pt server if necessary |
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192 | gb_error = arb_look_and_start_server(AISC_MAGIC_NUMBER, ptnam.str().c_str()); |
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193 | if (gb_error) { |
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194 | std::stringstream tmp; |
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195 | tmp << "Cannot contact PT server. Aborting" << endl |
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196 | << " ID: \"" << tmp.str().c_str() |
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197 | << "\" PORT: \"" << pt_server |
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198 | << "\" DB: \"" << pt_db << endl |
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199 | << "\" GBERROR: \"" << gb_error << "\"" << endl; |
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200 | gb_error = stream2static(tmp); |
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201 | } |
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202 | else { |
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203 | // create temporary file |
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204 | char* tmpfile; |
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205 | FILE* tmpfile_F; |
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206 | { |
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207 | char* tmpfile_tpl = GB_unique_filename("sina_select", "tmp"); |
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208 | tmpfile_F = GB_fopen_tempfile(tmpfile_tpl, "w", &tmpfile); |
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209 | free(tmpfile_tpl); |
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210 | } |
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211 | |
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212 | if (!tmpfile_F) { |
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213 | std::stringstream tmp; |
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214 | tmp << "Error: Unable to create temporary file \"" << tmpfile << "\"!"; |
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215 | gb_error = stream2static(tmp); |
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216 | } |
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217 | else { |
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218 | GB_remove_on_exit(tmpfile); |
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219 | |
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220 | std::vector<std::string> spec_names; |
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221 | switch (root->awar(GA_AWAR_TGT)->read_int()) { |
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222 | case 0: { // current |
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223 | char *spec_name = root->awar(AWAR_SPECIES_NAME)->read_string(); |
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224 | if (spec_name) { |
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225 | fwrite(spec_name, strlen(spec_name), 1, tmpfile_F); |
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226 | fwrite("\n", 1, 1, tmpfile_F); |
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227 | } |
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228 | else { |
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229 | gb_error = "Unable to get name of currently active species"; |
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230 | } |
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231 | free(spec_name); |
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232 | break; |
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233 | } |
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234 | case 1: { // selected |
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235 | GB_begin_transaction(GLOBAL_gb_main); |
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236 | int num_selected = 0; |
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237 | for (GBDATA *gb_spec = data_access->get_first_selected_species(&num_selected); |
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238 | gb_spec; |
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239 | gb_spec = data_access->get_next_selected_species()) |
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240 | { |
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241 | GBDATA *gbd_name = GB_find(gb_spec, "name", SEARCH_CHILD); |
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242 | if (gbd_name) { |
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243 | const char *str = GB_read_char_pntr(gbd_name); |
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244 | fwrite(str, strlen(str), 1, tmpfile_F); |
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245 | fwrite("\n", 1, 1, tmpfile_F); |
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246 | } |
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247 | } |
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248 | GB_commit_transaction(GLOBAL_gb_main); |
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249 | break; |
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250 | } |
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251 | case 2: { // marked |
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252 | GB_begin_transaction(GLOBAL_gb_main); |
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253 | for (GBDATA *gb_spec = GBT_first_marked_species(GLOBAL_gb_main); |
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254 | gb_spec; gb_spec = GBT_next_marked_species(gb_spec)) { |
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255 | GBDATA *gbd_name = GB_find(gb_spec, "name", SEARCH_CHILD); |
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256 | if (gbd_name) { |
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257 | const char *str = GB_read_char_pntr(gbd_name); |
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258 | fwrite(str, strlen(str), 1, tmpfile_F); |
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259 | fwrite("\n", 1, 1, tmpfile_F); |
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260 | } |
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261 | } |
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262 | GB_commit_transaction(GLOBAL_gb_main); |
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263 | break; |
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264 | } |
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265 | } |
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266 | fclose(tmpfile_F); |
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267 | |
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268 | if (!gb_error) { |
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269 | // build command line |
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270 | GBS_strstruct *cl = GBS_stropen(2000); |
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271 | |
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272 | GBS_strcat(cl, root->awar(GA_AWAR_CMD)->read_char_pntr()); |
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273 | GBS_strcat(cl, " -i :"); |
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274 | GBS_strcat(cl, " --ptdb :"); |
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275 | GBS_strcat(cl, " --ptport "); GBS_strcat(cl, pt_server); |
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276 | GBS_strcat(cl, " --turn "); GBS_strcat(cl, root->awar(GA_AWAR_TURN_CHECK)->read_int() ? "all" : "none"); |
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277 | GBS_strcat(cl, " --overhang "); GBS_strcat(cl, root->awar(GA_AWAR_OVERHANG)->read_char_pntr()); |
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278 | GBS_strcat(cl, " --filter "); GBS_strcat(cl, empty_as_none(root->awar(GA_AWAR_SAI)->read_char_pntr())); |
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279 | GBS_strcat(cl, " --fs-min "); GBS_intcat(cl, root->awar(GA_AWAR_FS_MIN)->read_int()); |
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280 | GBS_strcat(cl, " --fs-msc "); GBS_floatcat(cl, root->awar(GA_AWAR_FS_MSC)->read_float()); |
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281 | GBS_strcat(cl, " --fs-max "); GBS_intcat(cl, root->awar(GA_AWAR_FS_MAX)->read_int()); |
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282 | GBS_strcat(cl, " --fs-req 1"); |
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283 | GBS_strcat(cl, " --fs-req-full "); GBS_intcat(cl, root->awar(GA_AWAR_MIN_FULL)->read_int()); |
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284 | GBS_strcat(cl, " --fs-full-len "); GBS_intcat(cl, root->awar(GA_AWAR_FULL_MINLEN)->read_int()); |
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285 | GBS_strcat(cl, " --fs-kmer-len "); GBS_intcat(cl, root->awar(GA_AWAR_KMER_LEN)->read_int()); |
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286 | GBS_strcat(cl, " --fs-kmer-mm "); GBS_intcat(cl, root->awar(GA_AWAR_KMER_MM)->read_int()); |
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287 | GBS_strcat(cl, " --fs-min-len "); GBS_intcat(cl, root->awar(GA_AWAR_MIN_LEN)->read_int()); |
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288 | GBS_strcat(cl, " --fs-weight "); GBS_intcat(cl, root->awar(GA_AWAR_WEIGHT)->read_float()); // @@@ possible type conflict? |
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289 | GBS_strcat(cl, " --pen-gap "); GBS_floatcat(cl, root->awar(GA_AWAR_GAP_PEN)->read_float()); |
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290 | GBS_strcat(cl, " --pen-gapext "); GBS_floatcat(cl, root->awar(GA_AWAR_GAP_EXT)->read_float()); |
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291 | GBS_strcat(cl, " --match-score "); GBS_floatcat(cl, root->awar(GA_AWAR_MATCH_SCORE)->read_float()); |
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292 | GBS_strcat(cl, " --mismatch-score "); GBS_floatcat(cl, root->awar(GA_AWAR_MISMATCH_SCORE)->read_float()); |
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293 | GBS_strcat(cl, " --prot-level "); GBS_intcat(cl, root->awar(GA_AWAR_PROTECTION)->read_int()); |
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294 | GBS_strcat(cl, " --select-file "); GBS_strcat(cl, tmpfile); |
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295 | GBS_strcat(cl, " --insertion "); GBS_strcat(cl, root->awar(GA_AWAR_INSERT)->read_char_pntr()); |
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296 | GBS_strcat(cl, " --lowercase "); GBS_strcat(cl, root->awar(GA_AWAR_LOWERCASE)->read_char_pntr()); |
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297 | GBS_strcat(cl, " --auto-filter-field "); GBS_strcat(cl, root->awar(GA_AWAR_AUTOFILTER)->read_char_pntr()); |
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298 | GBS_strcat(cl, " --gene-start "); GBS_intcat(cl, root->awar(GA_AWAR_GENE_START)->read_int()); |
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299 | GBS_strcat(cl, " --gene-end "); GBS_intcat(cl, root->awar(GA_AWAR_GENE_END)->read_int()); |
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300 | GBS_strcat(cl, " --fs-cover-gene ");GBS_intcat(cl, root->awar(GA_AWAR_FS_COVER_GENE)->read_int()); |
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301 | |
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302 | |
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303 | if (root->awar(GA_AWAR_KMER_NOREL)->read_int()) GBS_strcat(cl, " --fs-kmer-norel "); |
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304 | if (root->awar(GA_AWAR_KMER_NOFAST)->read_int()) GBS_strcat(cl, " --fs-kmer-no-fast "); |
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305 | if (root->awar(GA_AWAR_SHOW_DIST)->read_int()) GBS_strcat(cl, " --show-dist "); |
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306 | if (root->awar(GA_AWAR_SHOW_DIFF)->read_int()) GBS_strcat(cl, " --show-diff "); |
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307 | if (root->awar(GA_AWAR_COLOR)->read_int()) GBS_strcat(cl, " --color"); |
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308 | if (root->awar(GA_AWAR_REALIGN)->read_int()) GBS_strcat(cl, " --realign"); |
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309 | |
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310 | gb_error = GB_xcmd(GBS_mempntr(cl), XCMD_ASYNC_WAITKEY); |
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311 | GBS_strforget(cl); |
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312 | } |
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313 | |
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314 | if (!gb_error) aw_message("SINA finished aligning."); |
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315 | } |
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316 | free(tmpfile); |
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317 | } |
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318 | } |
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319 | |
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320 | aw_message_if(gb_error); |
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321 | } |
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322 | |
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323 | |
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324 | |
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325 | static char* filter_posvar_SAI_for_ali(GBDATA *gb_extended, const char *ali_name) { |
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326 | char *result = NULp; |
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327 | char *typePath = GBS_global_string_copy("%s/_TYPE", ali_name); |
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328 | GBDATA *gbd = GB_search(gb_extended, typePath, GB_FIND); |
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329 | if (gbd) { |
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330 | const char* type = GB_read_char_pntr(gbd); |
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331 | if (type && strncmp("PV", type, 2) == 0) { |
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332 | gbd = GB_find(gb_extended, "name", SEARCH_CHILD); |
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333 | result = GB_read_string(gbd); |
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334 | } |
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335 | } |
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336 | free(typePath); |
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337 | return result; |
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338 | } |
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339 | |
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340 | static AWT_config_mapping_def sina_config_mapping[] = { |
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341 | // both |
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342 | { GA_AWAR_TGT, "target" }, |
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343 | { GA_AWAR_OVERHANG, "overhang" }, |
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344 | { GA_AWAR_INSERT, "insertions" }, |
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345 | { GA_AWAR_LOWERCASE, "lowercase" }, |
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346 | { GA_AWAR_WEIGHT, "weight" }, |
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347 | { GA_AWAR_FULL_MINLEN, "fullminlen" }, |
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348 | |
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349 | // advanced only |
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350 | { GA_AWAR_SAI, "sai" }, |
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351 | { GA_AWAR_AUTOFILTER, "autofilter" }, |
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352 | { GA_AWAR_TURN_CHECK, "turncheck" }, |
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353 | { GA_AWAR_REALIGN, "realign" }, |
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354 | { GA_AWAR_GAP_PEN, "gappen" }, |
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355 | { GA_AWAR_GAP_EXT, "gapext" }, |
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356 | { GA_AWAR_MATCH_SCORE, "matchscore" }, |
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357 | { GA_AWAR_MISMATCH_SCORE, "mismatchscore" }, |
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358 | { GA_AWAR_FS_MIN, "fs_min" }, |
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359 | { GA_AWAR_FS_MSC, "fs_minscore" }, |
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360 | { GA_AWAR_FS_MAX, "fs_min" }, |
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361 | { GA_AWAR_MIN_FULL, "minfull" }, |
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362 | { GA_AWAR_KMER_LEN, "kmerlen" }, |
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363 | { GA_AWAR_KMER_MM, "kmermm" }, |
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364 | { GA_AWAR_MIN_LEN, "refminlen" }, |
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365 | { GA_AWAR_GENE_START, "genestart" }, |
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366 | { GA_AWAR_GENE_END, "geneend" }, |
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367 | { GA_AWAR_FS_COVER_GENE, "fs_covergene" }, |
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368 | { GA_AWAR_KMER_NOFAST, "kmernofast" }, |
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369 | { GA_AWAR_KMER_NOREL, "kmernorel" }, |
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370 | |
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371 | // both |
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372 | { GA_AWAR_SHOW_DIFF, "showdiff" }, |
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373 | { GA_AWAR_COLOR, "color" }, |
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374 | { GA_AWAR_SHOW_DIST, "showdist" }, |
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375 | |
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376 | { NULp, NULp } |
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377 | }; |
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378 | |
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379 | static AW_window_simple* new_sina_simple(AW_root *root, const AlignDataAccess *alignData, bool adv) { |
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380 | int closex, closey, startx, starty, winx, winy; |
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381 | const int hgap = 10; |
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382 | AW_window_simple *aws = new AW_window_simple; |
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383 | |
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384 | aws->init(root, adv ? "XSINA" : "SINA", "SINA (SILVA Incremental Aligner)"); |
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385 | |
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386 | aws->button_length(12); |
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387 | aws->at(10, 10); |
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388 | aws->auto_space(5, 5); |
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389 | |
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390 | aws->callback(AW_POPDOWN); |
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391 | aws->create_button("CLOSE", "CLOSE", "O"); |
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392 | aws->get_at_position(&closex, &closey); |
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393 | |
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394 | aws->at_shift(10, 0); |
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395 | aws->label_length(0); |
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396 | aws->label("Show advanced options"); |
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397 | aws->create_toggle(GA_AWAR_ADVANCED); |
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398 | |
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399 | bool cbInstalled = false; |
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400 | if (!cbInstalled) { |
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401 | root->awar(GA_AWAR_ADVANCED)->add_callback(makeRootCallback(show_sina_window, alignData)); |
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402 | cbInstalled = true; |
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403 | } |
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404 | |
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405 | aws->at_newline(); |
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406 | aws->at_shift(0, hgap); |
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407 | aws->label_length(15); |
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408 | aws->create_toggle_field(GA_AWAR_TGT, "Align what?", "A"); |
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409 | aws->insert_toggle("Current Species:", "C", 0); |
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410 | aws->insert_toggle("Selected Species", "S", 1); |
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411 | aws->insert_default_toggle("Marked Species", "M", 2); |
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412 | aws->update_toggle_field(); |
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413 | |
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414 | aws->at_shift(0, 3); |
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415 | aws->create_input_field(AWAR_SPECIES_NAME, 20); |
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416 | |
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417 | aws->at_newline(); |
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418 | aws->at_shift(0, hgap); |
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419 | aws->button_length(24); |
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420 | aws->label("PT Server:"); |
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421 | awt_create_PTSERVER_selection_button(aws, AWAR_PT_SERVER); |
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422 | |
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423 | aws->at_newline(); |
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424 | aws->label_length(0); |
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425 | aws->label("Overhang placement"); |
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426 | aws->create_option_menu(GA_AWAR_OVERHANG, true); |
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427 | aws->insert_option("keep attached", NULp, "attach"); |
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428 | aws->insert_option("move to edge", NULp, "edge"); |
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429 | aws->insert_option("remove", NULp, "remove"); |
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430 | aws->update_option_menu(); |
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431 | |
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432 | aws->at_newline(); |
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433 | aws->label("Handling of unmappable insertions"); |
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434 | aws->create_option_menu(GA_AWAR_INSERT, true); |
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435 | aws->insert_option("Shift surrounding bases", NULp, "shift"); |
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436 | aws->insert_option("Forbid during DP alignment", NULp, "forbid"); |
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437 | aws->insert_option("Delete bases", NULp, "remove"); |
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438 | aws->update_option_menu(); |
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439 | |
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440 | aws->at_newline(); |
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441 | aws->label("Character Case"); |
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442 | aws->create_option_menu(GA_AWAR_LOWERCASE, true); |
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443 | aws->insert_option("Do not modify", NULp, "original"); |
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444 | aws->insert_option("Show unaligned bases as lower case", NULp, "unaligned"); |
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445 | aws->insert_option("Uppercase all", NULp, "none"); |
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446 | aws->update_option_menu(); |
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447 | |
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448 | aws->at_newline(); |
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449 | aws->label("Family conservation weight (0-1)"); |
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450 | aws->create_input_field(GA_AWAR_WEIGHT, 3); |
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451 | |
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452 | aws->at_newline(); |
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453 | aws->label("Size of full-length sequences"); |
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454 | aws->create_input_field(GA_AWAR_FULL_MINLEN, 5); |
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455 | |
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456 | if (adv) { |
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457 | aws->at_newline(); |
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458 | aws->at_shift(0, hgap); |
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459 | |
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460 | aws->at_newline(); |
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461 | |
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462 | aws->label("Pos. Var.:"); |
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463 | awt_create_SAI_selection_button(GLOBAL_gb_main, aws, GA_AWAR_SAI, makeSaiSelectionlistFilterCallback(filter_posvar_SAI_for_ali, alignData->alignment_name.c_str())); |
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464 | |
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465 | aws->at_newline(); |
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466 | aws->label("Field used for automatic filter selection"); |
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467 | aws->create_input_field(GA_AWAR_AUTOFILTER, 20); |
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468 | |
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469 | aws->label("Turn check"); |
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470 | aws->create_toggle(GA_AWAR_TURN_CHECK); |
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471 | |
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472 | aws->at_newline(); |
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473 | aws->label("Realign"); |
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474 | aws->create_toggle(GA_AWAR_REALIGN); |
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475 | |
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476 | /* |
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477 | aws->at_newline(); |
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478 | aws->label("(Copy and) mark sequences used as reference"); |
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479 | aws->create_toggle(GA_AWAR_COPYMARKREF); |
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480 | */ |
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481 | |
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482 | aws->at_newline(); |
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483 | aws->at_shift(0, hgap); |
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484 | aws->label_length(0); |
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485 | |
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486 | aws->label("Gap insertion/extension penalties"); |
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487 | aws->create_input_field(GA_AWAR_GAP_PEN, 5); |
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488 | aws->create_input_field(GA_AWAR_GAP_EXT, 5); |
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489 | |
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490 | aws->at_newline(); |
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491 | aws->label("Match score"); |
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492 | aws->create_input_field(GA_AWAR_MATCH_SCORE, 3); |
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493 | aws->label("Mismatch score"); |
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494 | aws->create_input_field(GA_AWAR_MISMATCH_SCORE, 3); |
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495 | |
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496 | aws->at_newline(); |
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497 | aws->label("Family search min/min_score/max"); |
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498 | aws->create_input_field(GA_AWAR_FS_MIN, 3); |
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499 | aws->create_input_field(GA_AWAR_FS_MSC, 3); |
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500 | aws->create_input_field(GA_AWAR_FS_MAX, 3); |
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501 | |
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502 | aws->at_newline(); |
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503 | aws->label("Minimal number of full length sequences"); |
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504 | aws->create_input_field(GA_AWAR_MIN_FULL, 3); |
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505 | |
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506 | aws->at_newline(); |
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507 | aws->label("Family search oligo length / mismatches"); |
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508 | aws->create_input_field(GA_AWAR_KMER_LEN, 3); |
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509 | aws->create_input_field(GA_AWAR_KMER_MM, 3); |
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510 | |
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511 | aws->at_newline(); |
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512 | aws->label("Minimal reference sequence length"); |
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513 | aws->create_input_field(GA_AWAR_MIN_LEN, 5); |
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514 | |
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515 | aws->at_newline(); |
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516 | aws->label("Alignment bounds: start"); |
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517 | aws->create_input_field(GA_AWAR_GENE_START, 6); |
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518 | aws->label("end"); |
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519 | aws->create_input_field(GA_AWAR_GENE_END, 6); |
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520 | |
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521 | aws->at_newline(); |
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522 | aws->label("Number of references required to touch bounds"); |
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523 | aws->create_input_field(GA_AWAR_FS_COVER_GENE, 3); |
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524 | |
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525 | aws->at_newline(); |
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526 | aws->label("Disable fast search"); |
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527 | aws->create_toggle(GA_AWAR_KMER_NOFAST); |
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528 | |
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529 | aws->at_newline(); |
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530 | aws->label("Score search results by absolute oligo match count"); |
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531 | aws->create_toggle(GA_AWAR_KMER_NOREL); |
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532 | |
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533 | aws->at_newline(); |
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534 | aws->label("SINA command"); |
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535 | aws->create_input_field(GA_AWAR_CMD, 20); |
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536 | |
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537 | aws->at_shift(0, hgap); |
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538 | } |
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539 | |
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540 | aws->at_newline(); |
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541 | aws->at_shift(0, hgap); |
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542 | |
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543 | aws->label_length(17); |
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544 | aws->label("Protection Level"); |
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545 | aws->create_option_menu(GA_AWAR_PROTECTION, true); |
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546 | aws->insert_option("0", NULp, 0); |
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547 | aws->insert_option("1", NULp, 1); |
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548 | aws->insert_option("2", NULp, 2); |
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549 | aws->insert_option("3", NULp, 3); |
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550 | aws->insert_option("4", NULp, 4); |
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551 | aws->insert_option("5", NULp, 5); |
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552 | aws->insert_option("6", NULp, 6); |
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553 | aws->update_option_menu(); |
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554 | |
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555 | aws->at_newline(); |
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556 | aws->label("Show changed sections of alignment"); |
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557 | aws->create_toggle(GA_AWAR_SHOW_DIFF); |
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558 | aws->label("color bases"); |
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559 | aws->create_toggle(GA_AWAR_COLOR); |
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560 | |
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561 | aws->at_newline(); |
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562 | aws->label("Show statistics"); |
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563 | aws->create_toggle(GA_AWAR_SHOW_DIST); |
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564 | |
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565 | aws->get_window_size(winx, winy); |
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566 | aws->get_at_position(&startx, &starty); |
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567 | |
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568 | aws->button_length(12); |
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569 | |
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570 | aws->at(winx-closex+5, closey); |
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571 | aws->callback(makeHelpCallback("sina_main.hlp")); |
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572 | aws->create_button("HELP", "HELP"); |
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573 | |
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574 | aws->at(winx-closex+5, starty); |
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575 | aws->callback(makeWindowCallback(sina_start, alignData)); |
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576 | aws->highlight(); |
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577 | aws->create_button("Start", "Start", "S"); |
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578 | |
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579 | aws->at(winx-50, starty-50); |
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580 | AWT_insert_config_manager(aws, AW_ROOT_DEFAULT, "sina", sina_config_mapping); |
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581 | |
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582 | return aws; |
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583 | } |
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584 | |
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585 | void show_sina_window(UNFIXED, const AlignDataAccess *alignData) { |
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586 | AW_root *root = AW_root::SINGLETON; |
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587 | |
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588 | static AW_window_simple *ga_aws = new_sina_simple(root, alignData, false); |
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589 | static AW_window_simple *ga_aws_adv = new_sina_simple(root, alignData, true); |
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590 | |
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591 | if (root->awar(GA_AWAR_ADVANCED)->read_int()) { |
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592 | ga_aws_adv->show(); |
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593 | ga_aws->hide(); |
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594 | } |
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595 | else { |
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596 | ga_aws->show(); |
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597 | ga_aws_adv->hide(); |
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598 | } |
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599 | } |
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600 | |
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