| 1 | // =============================================================== // |
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| 2 | // // |
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| 3 | // File : graph_aligner_gui.cxx // |
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| 4 | // Purpose : // |
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| 5 | // // |
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| 6 | // Coded by Elmar Pruesse in October 2008 // |
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| 7 | // Institute of Microbiology (Technical University Munich) // |
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| 8 | // http://www.arb-home.de/ // |
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| 9 | // // |
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| 10 | // =============================================================== // |
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| 11 | |
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| 12 | #include "graph_aligner_gui.hxx" |
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| 13 | |
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| 14 | #include "ed4_defs.hxx" |
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| 15 | |
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| 16 | // need to use AlignDataAccess defined here to get selected species |
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| 17 | #include <fast_aligner.hxx> |
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| 18 | |
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| 19 | #include <aw_awars.hxx> |
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| 20 | #include <aw_msg.hxx> |
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| 21 | #include <aw_root.hxx> |
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| 22 | #include <awt_sel_boxes.hxx> |
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| 23 | #include <awt_config_manager.hxx> |
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| 24 | #include <servercntrl.h> |
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| 25 | #include <PT_com.h> |
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| 26 | #include <client.h> |
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| 27 | #include <arbdbt.h> |
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| 28 | #include <arb_strbuf.h> |
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| 29 | #include <arb_misc.h> |
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| 30 | |
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| 31 | #include <sys/types.h> |
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| 32 | #include <sys/wait.h> |
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| 33 | |
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| 34 | #include <unistd.h> |
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| 35 | |
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| 36 | #include <string> |
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| 37 | #include <sstream> |
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| 38 | #include <iostream> |
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| 39 | #include <vector> |
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| 40 | |
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| 41 | using std::cerr; |
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| 42 | using std::cout; |
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| 43 | using std::endl; |
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| 44 | |
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| 45 | #define GA_AWAR_ROOT "sina/" |
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| 46 | |
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| 47 | #define GA_AWAR_CMD GA_AWAR_ROOT "command" |
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| 48 | #define GA_AWAR_TGT GA_AWAR_ROOT "target" |
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| 49 | #define GA_AWAR_SAI GA_AWAR_ROOT "sai" |
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| 50 | #define GA_AWAR_ALIGNMENT GA_AWAR_ROOT "alignment" |
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| 51 | #define GA_AWAR_PROTECTION GA_AWAR_ROOT "protection" |
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| 52 | #define GA_AWAR_TURN_CHECK GA_AWAR_ROOT "turncheck" |
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| 53 | #define GA_AWAR_REALIGN GA_AWAR_ROOT "realign" |
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| 54 | #define GA_AWAR_COPYMARKREF GA_AWAR_ROOT "copymarkref" |
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| 55 | #define GA_AWAR_MATCH_SCORE GA_AWAR_ROOT "match_score" |
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| 56 | #define GA_AWAR_MISMATCH_SCORE GA_AWAR_ROOT "mismatch_score" |
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| 57 | #define GA_AWAR_GAP_PEN GA_AWAR_ROOT "gap_pen" |
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| 58 | #define GA_AWAR_GAP_EXT GA_AWAR_ROOT "gap_ext" |
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| 59 | #define GA_AWAR_ADVANCED GA_AWAR_ROOT "advanced" |
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| 60 | #define GA_AWAR_FS_MIN GA_AWAR_ROOT "fs_min" |
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| 61 | #define GA_AWAR_FS_MAX GA_AWAR_ROOT "fs_max" |
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| 62 | #define GA_AWAR_FS_MSC GA_AWAR_ROOT "fs_msc" |
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| 63 | #define GA_AWAR_MIN_FULL GA_AWAR_ROOT "min_full" |
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| 64 | #define GA_AWAR_FULL_MINLEN GA_AWAR_ROOT "full_minlen" |
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| 65 | #define GA_AWAR_OVERHANG GA_AWAR_ROOT "overhang" |
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| 66 | #define GA_AWAR_THREADS GA_AWAR_ROOT "threads" |
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| 67 | #define GA_AWAR_QSIZE GA_AWAR_ROOT "qsize" |
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| 68 | #define GA_AWAR_KMER_LEN GA_AWAR_ROOT "kmer_len" |
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| 69 | #define GA_AWAR_KMER_MM GA_AWAR_ROOT "kmer_mm" |
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| 70 | #define GA_AWAR_MIN_LEN GA_AWAR_ROOT "min_len" |
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| 71 | #define GA_AWAR_WEIGHT GA_AWAR_ROOT "weight" |
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| 72 | #define GA_AWAR_INSERT GA_AWAR_ROOT "insert" |
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| 73 | #define GA_AWAR_LOWERCASE GA_AWAR_ROOT "lowercase" |
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| 74 | #define GA_AWAR_AUTOFILTER GA_AWAR_ROOT "autofilter" |
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| 75 | #define GA_AWAR_KMER_NOREL GA_AWAR_ROOT "kmer_norel" |
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| 76 | #define GA_AWAR_KMER_NOFAST GA_AWAR_ROOT "kmer_nofast" |
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| 77 | #define GA_AWAR_SHOW_DIST GA_AWAR_ROOT "show_dist" |
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| 78 | #define GA_AWAR_SHOW_DIFF GA_AWAR_ROOT "show_diff" |
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| 79 | #define GA_AWAR_COLOR GA_AWAR_ROOT "color" |
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| 80 | #define GA_AWAR_GENE_START GA_AWAR_ROOT "gene_start" |
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| 81 | #define GA_AWAR_GENE_END GA_AWAR_ROOT "gene_end" |
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| 82 | #define GA_AWAR_FS_COVER_GENE GA_AWAR_ROOT "fs_cover_gene" |
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| 83 | |
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| 84 | void create_sina_variables(AW_root *root, AW_default db1) { |
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| 85 | root->awar_string(GA_AWAR_CMD, "sina", db1); |
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| 86 | root->awar_int (GA_AWAR_TGT, 2, db1); |
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| 87 | root->awar_int (AWAR_PT_SERVER, 0, db1); |
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| 88 | root->awar_string(GA_AWAR_SAI, "", db1); |
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| 89 | root->awar_int (GA_AWAR_PROTECTION, 0, db1); |
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| 90 | root->awar_int (GA_AWAR_TURN_CHECK, 1, db1); |
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| 91 | root->awar_int (GA_AWAR_REALIGN, 1, db1); |
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| 92 | root->awar_int (GA_AWAR_COPYMARKREF, 0, db1); |
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| 93 | root->awar_float (GA_AWAR_GAP_PEN, 5.0, db1); |
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| 94 | root->awar_float (GA_AWAR_GAP_EXT, 2.0, db1); |
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| 95 | root->awar_float (GA_AWAR_MATCH_SCORE, 2.0, db1); |
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| 96 | root->awar_float (GA_AWAR_MISMATCH_SCORE, -1.0, db1); |
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| 97 | root->awar_int (GA_AWAR_ADVANCED, 0, db1); |
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| 98 | root->awar_int (GA_AWAR_FS_MIN, 40, db1); |
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| 99 | root->awar_int (GA_AWAR_FS_MAX, 40, db1); |
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| 100 | root->awar_float (GA_AWAR_FS_MSC, .7, db1); |
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| 101 | root->awar_int (GA_AWAR_MIN_FULL, 1, db1); |
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| 102 | root->awar_int (GA_AWAR_FULL_MINLEN, 1400, db1); |
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| 103 | root->awar_string(GA_AWAR_OVERHANG, "attach", db1); |
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| 104 | root->awar_int (GA_AWAR_THREADS, 1, db1); |
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| 105 | root->awar_int (GA_AWAR_QSIZE, 1, db1); |
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| 106 | root->awar_int (GA_AWAR_KMER_LEN, 10, db1); |
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| 107 | root->awar_int (GA_AWAR_KMER_MM, 0, db1); |
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| 108 | root->awar_int (GA_AWAR_MIN_LEN, 150, db1); |
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| 109 | root->awar_float (GA_AWAR_WEIGHT, 1, db1); |
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| 110 | root->awar_string(GA_AWAR_INSERT, "shift", db1); |
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| 111 | root->awar_string(GA_AWAR_LOWERCASE, "none", db1); |
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| 112 | root->awar_string(GA_AWAR_AUTOFILTER, "none", db1); |
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| 113 | root->awar_int (GA_AWAR_KMER_NOREL, 0, db1); |
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| 114 | root->awar_int (GA_AWAR_KMER_NOFAST, 0, db1); |
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| 115 | root->awar_int (GA_AWAR_SHOW_DIST, 0, db1); |
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| 116 | root->awar_int (GA_AWAR_SHOW_DIFF, 0, db1); |
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| 117 | root->awar_int (GA_AWAR_COLOR, 1, db1); |
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| 118 | root->awar_int (GA_AWAR_GENE_START, 0, db1); |
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| 119 | root->awar_int (GA_AWAR_GENE_END, 0, db1); |
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| 120 | root->awar_int (GA_AWAR_FS_COVER_GENE, 1, db1); |
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| 121 | } |
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| 122 | |
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| 123 | AW_active sina_mask(AW_root *root) { |
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| 124 | const char *sinaName = root->awar(GA_AWAR_CMD)->read_char_pntr(); |
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| 125 | char *sina = ARB_executable(sinaName, GB_getenv("PATH")); |
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| 126 | const char *fail_reason = NULp; |
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| 127 | |
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| 128 | if (sina) { |
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| 129 | int exitstatus = system(GBS_global_string("%s --has-cli-vers ARB5.99", sina)); |
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| 130 | exitstatus = WEXITSTATUS(exitstatus); |
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| 131 | |
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| 132 | switch (exitstatus) { |
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| 133 | case EXIT_SUCCESS: |
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| 134 | break; |
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| 135 | |
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| 136 | case EXIT_FAILURE: |
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| 137 | fail_reason = "Incompatible SINA and ARB versions"; |
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| 138 | break; |
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| 139 | |
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| 140 | case 127: // libs missing |
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| 141 | default: // unexpected |
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| 142 | fail_reason = GBS_global_string("Could execute SINA binary '%s' (exitstatus was %i)", |
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| 143 | sinaName, exitstatus); |
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| 144 | break; |
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| 145 | } |
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| 146 | free(sina); |
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| 147 | } |
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| 148 | else { |
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| 149 | fail_reason = GBS_global_string("%s not found", sinaName); |
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| 150 | } |
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| 151 | |
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| 152 | if (fail_reason) { |
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| 153 | fprintf(stderr, |
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| 154 | "Note: SINA (SILVA Aligner) disabled (Reason: %s)\n", |
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| 155 | fail_reason); |
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| 156 | } |
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| 157 | |
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| 158 | return fail_reason ? AWM_DISABLED : AWM_ALL; |
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| 159 | } |
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| 160 | |
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| 161 | inline const char *stream2static(const std::stringstream& str) { |
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| 162 | return GBS_static_string(str.str().c_str()); |
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| 163 | } |
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| 164 | |
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| 165 | inline const char *empty_as_none(const char *sainame) { |
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| 166 | // see http://bugs.arb-home.de/ticket/519 |
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| 167 | if (sainame && !sainame[0]) sainame = "none"; |
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| 168 | return sainame; |
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| 169 | } |
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| 170 | |
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| 171 | static void sina_start(AW_window *window, const AlignDataAccess *data_access) { |
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| 172 | cerr << "Starting SINA..." << endl; |
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| 173 | |
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| 174 | // make string from pt server selection |
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| 175 | AW_root *root = window->get_root(); |
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| 176 | int pt = root->awar(AWAR_PT_SERVER)->read_int(); |
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| 177 | |
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| 178 | std::stringstream ptnam; |
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| 179 | ptnam << "ARB_PT_SERVER" << pt; |
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| 180 | |
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| 181 | const char *pt_server = GBS_read_arb_tcp(ptnam.str().c_str()); |
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| 182 | GB_ERROR gb_error = NULp; |
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| 183 | |
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| 184 | if (!pt_server) { |
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| 185 | gb_error = GBS_global_string("Unable to find definition for chosen PT-server\n(Reason: %s)", GB_await_error()); |
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| 186 | } |
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| 187 | else { |
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| 188 | const char *pt_db = pt_server + strlen(pt_server) + 1; |
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| 189 | pt_db += strlen(pt_db)+3; |
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| 190 | |
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| 191 | // start pt server if necessary |
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| 192 | gb_error = arb_look_and_start_server(AISC_MAGIC_NUMBER, ptnam.str().c_str()); |
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| 193 | if (gb_error) { |
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| 194 | std::stringstream tmp; |
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| 195 | tmp << "Cannot contact PT server. Aborting" << endl |
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| 196 | << " ID: \"" << tmp.str().c_str() |
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| 197 | << "\" PORT: \"" << pt_server |
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| 198 | << "\" DB: \"" << pt_db << endl |
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| 199 | << "\" GBERROR: \"" << gb_error << "\"" << endl; |
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| 200 | gb_error = stream2static(tmp); |
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| 201 | } |
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| 202 | else { |
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| 203 | // create temporary file |
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| 204 | char* tmpfile; |
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| 205 | FILE* tmpfile_F; |
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| 206 | { |
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| 207 | char* tmpfile_tpl = GB_unique_filename("sina_select", "tmp"); |
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| 208 | tmpfile_F = GB_fopen_tempfile(tmpfile_tpl, "w", &tmpfile); |
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| 209 | free(tmpfile_tpl); |
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| 210 | } |
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| 211 | |
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| 212 | if (!tmpfile_F) { |
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| 213 | std::stringstream tmp; |
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| 214 | tmp << "Error: Unable to create temporary file \"" << tmpfile << "\"!"; |
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| 215 | gb_error = stream2static(tmp); |
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| 216 | } |
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| 217 | else { |
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| 218 | GB_remove_on_exit(tmpfile); |
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| 219 | |
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| 220 | std::vector<std::string> spec_names; |
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| 221 | switch (root->awar(GA_AWAR_TGT)->read_int()) { |
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| 222 | case 0: { // current |
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| 223 | char *spec_name = root->awar(AWAR_SPECIES_NAME)->read_string(); |
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| 224 | if (spec_name) { |
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| 225 | fwrite(spec_name, strlen(spec_name), 1, tmpfile_F); |
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| 226 | fwrite("\n", 1, 1, tmpfile_F); |
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| 227 | } |
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| 228 | else { |
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| 229 | gb_error = "Unable to get name of currently active species"; |
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| 230 | } |
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| 231 | free(spec_name); |
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| 232 | break; |
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| 233 | } |
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| 234 | case 1: { // selected |
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| 235 | GB_begin_transaction(GLOBAL_gb_main); |
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| 236 | int num_selected = 0; |
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| 237 | for (GBDATA *gb_spec = data_access->get_first_selected_species(&num_selected); |
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| 238 | gb_spec; |
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| 239 | gb_spec = data_access->get_next_selected_species()) |
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| 240 | { |
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| 241 | GBDATA *gbd_name = GB_find(gb_spec, "name", SEARCH_CHILD); |
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| 242 | if (gbd_name) { |
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| 243 | const char *str = GB_read_char_pntr(gbd_name); |
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| 244 | fwrite(str, strlen(str), 1, tmpfile_F); |
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| 245 | fwrite("\n", 1, 1, tmpfile_F); |
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| 246 | } |
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| 247 | } |
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| 248 | GB_commit_transaction(GLOBAL_gb_main); |
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| 249 | break; |
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| 250 | } |
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| 251 | case 2: { // marked |
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| 252 | GB_begin_transaction(GLOBAL_gb_main); |
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| 253 | for (GBDATA *gb_spec = GBT_first_marked_species(GLOBAL_gb_main); |
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| 254 | gb_spec; gb_spec = GBT_next_marked_species(gb_spec)) { |
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| 255 | GBDATA *gbd_name = GB_find(gb_spec, "name", SEARCH_CHILD); |
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| 256 | if (gbd_name) { |
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| 257 | const char *str = GB_read_char_pntr(gbd_name); |
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| 258 | fwrite(str, strlen(str), 1, tmpfile_F); |
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| 259 | fwrite("\n", 1, 1, tmpfile_F); |
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| 260 | } |
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| 261 | } |
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| 262 | GB_commit_transaction(GLOBAL_gb_main); |
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| 263 | break; |
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| 264 | } |
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| 265 | } |
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| 266 | fclose(tmpfile_F); |
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| 267 | |
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| 268 | if (!gb_error) { |
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| 269 | // build command line |
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| 270 | GBS_strstruct *cl = GBS_stropen(2000); |
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| 271 | |
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| 272 | GBS_strcat(cl, root->awar(GA_AWAR_CMD)->read_char_pntr()); |
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| 273 | GBS_strcat(cl, " -i :"); |
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| 274 | GBS_strcat(cl, " --ptdb :"); |
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| 275 | GBS_strcat(cl, " --ptport "); GBS_strcat(cl, pt_server); |
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| 276 | GBS_strcat(cl, " --turn "); GBS_strcat(cl, root->awar(GA_AWAR_TURN_CHECK)->read_int() ? "all" : "none"); |
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| 277 | GBS_strcat(cl, " --overhang "); GBS_strcat(cl, root->awar(GA_AWAR_OVERHANG)->read_char_pntr()); |
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| 278 | GBS_strcat(cl, " --filter "); GBS_strcat(cl, empty_as_none(root->awar(GA_AWAR_SAI)->read_char_pntr())); |
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| 279 | GBS_strcat(cl, " --fs-min "); GBS_intcat(cl, root->awar(GA_AWAR_FS_MIN)->read_int()); |
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| 280 | GBS_strcat(cl, " --fs-msc "); GBS_floatcat(cl, root->awar(GA_AWAR_FS_MSC)->read_float()); |
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| 281 | GBS_strcat(cl, " --fs-max "); GBS_intcat(cl, root->awar(GA_AWAR_FS_MAX)->read_int()); |
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| 282 | GBS_strcat(cl, " --fs-req 1"); |
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| 283 | GBS_strcat(cl, " --fs-req-full "); GBS_intcat(cl, root->awar(GA_AWAR_MIN_FULL)->read_int()); |
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| 284 | GBS_strcat(cl, " --fs-full-len "); GBS_intcat(cl, root->awar(GA_AWAR_FULL_MINLEN)->read_int()); |
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| 285 | GBS_strcat(cl, " --fs-kmer-len "); GBS_intcat(cl, root->awar(GA_AWAR_KMER_LEN)->read_int()); |
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| 286 | GBS_strcat(cl, " --fs-kmer-mm "); GBS_intcat(cl, root->awar(GA_AWAR_KMER_MM)->read_int()); |
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| 287 | GBS_strcat(cl, " --fs-min-len "); GBS_intcat(cl, root->awar(GA_AWAR_MIN_LEN)->read_int()); |
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| 288 | GBS_strcat(cl, " --fs-weight "); GBS_intcat(cl, root->awar(GA_AWAR_WEIGHT)->read_float()); // @@@ possible type conflict? |
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| 289 | GBS_strcat(cl, " --pen-gap "); GBS_floatcat(cl, root->awar(GA_AWAR_GAP_PEN)->read_float()); |
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| 290 | GBS_strcat(cl, " --pen-gapext "); GBS_floatcat(cl, root->awar(GA_AWAR_GAP_EXT)->read_float()); |
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| 291 | GBS_strcat(cl, " --match-score "); GBS_floatcat(cl, root->awar(GA_AWAR_MATCH_SCORE)->read_float()); |
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| 292 | GBS_strcat(cl, " --mismatch-score "); GBS_floatcat(cl, root->awar(GA_AWAR_MISMATCH_SCORE)->read_float()); |
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| 293 | GBS_strcat(cl, " --prot-level "); GBS_intcat(cl, root->awar(GA_AWAR_PROTECTION)->read_int()); |
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| 294 | GBS_strcat(cl, " --select-file "); GBS_strcat(cl, tmpfile); |
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| 295 | GBS_strcat(cl, " --insertion "); GBS_strcat(cl, root->awar(GA_AWAR_INSERT)->read_char_pntr()); |
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| 296 | GBS_strcat(cl, " --lowercase "); GBS_strcat(cl, root->awar(GA_AWAR_LOWERCASE)->read_char_pntr()); |
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| 297 | GBS_strcat(cl, " --auto-filter-field "); GBS_strcat(cl, root->awar(GA_AWAR_AUTOFILTER)->read_char_pntr()); |
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| 298 | GBS_strcat(cl, " --gene-start "); GBS_intcat(cl, root->awar(GA_AWAR_GENE_START)->read_int()); |
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| 299 | GBS_strcat(cl, " --gene-end "); GBS_intcat(cl, root->awar(GA_AWAR_GENE_END)->read_int()); |
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| 300 | GBS_strcat(cl, " --fs-cover-gene ");GBS_intcat(cl, root->awar(GA_AWAR_FS_COVER_GENE)->read_int()); |
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| 301 | |
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| 302 | |
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| 303 | if (root->awar(GA_AWAR_KMER_NOREL)->read_int()) GBS_strcat(cl, " --fs-kmer-norel "); |
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| 304 | if (root->awar(GA_AWAR_KMER_NOFAST)->read_int()) GBS_strcat(cl, " --fs-kmer-no-fast "); |
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| 305 | if (root->awar(GA_AWAR_SHOW_DIST)->read_int()) GBS_strcat(cl, " --show-dist "); |
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| 306 | if (root->awar(GA_AWAR_SHOW_DIFF)->read_int()) GBS_strcat(cl, " --show-diff "); |
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| 307 | if (root->awar(GA_AWAR_COLOR)->read_int()) GBS_strcat(cl, " --color"); |
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| 308 | if (root->awar(GA_AWAR_REALIGN)->read_int()) GBS_strcat(cl, " --realign"); |
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| 309 | |
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| 310 | gb_error = GB_xcmd(GBS_mempntr(cl), XCMD_ASYNC_WAITKEY); |
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| 311 | GBS_strforget(cl); |
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| 312 | } |
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| 313 | |
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| 314 | if (!gb_error) aw_message("SINA finished aligning."); |
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| 315 | } |
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| 316 | free(tmpfile); |
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| 317 | } |
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| 318 | } |
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| 319 | |
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| 320 | aw_message_if(gb_error); |
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| 321 | } |
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| 322 | |
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| 323 | |
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| 324 | |
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| 325 | static char* filter_posvar_SAI_for_ali(GBDATA *gb_extended, const char *ali_name) { |
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| 326 | char *result = NULp; |
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| 327 | char *typePath = GBS_global_string_copy("%s/_TYPE", ali_name); |
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| 328 | GBDATA *gbd = GB_search(gb_extended, typePath, GB_FIND); |
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| 329 | if (gbd) { |
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| 330 | const char* type = GB_read_char_pntr(gbd); |
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| 331 | if (type && strncmp("PV", type, 2) == 0) { |
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| 332 | gbd = GB_find(gb_extended, "name", SEARCH_CHILD); |
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| 333 | result = GB_read_string(gbd); |
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| 334 | } |
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| 335 | } |
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| 336 | free(typePath); |
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| 337 | return result; |
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| 338 | } |
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| 339 | |
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| 340 | static AWT_config_mapping_def sina_config_mapping[] = { |
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| 341 | // both |
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| 342 | { GA_AWAR_TGT, "target" }, |
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| 343 | { GA_AWAR_OVERHANG, "overhang" }, |
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| 344 | { GA_AWAR_INSERT, "insertions" }, |
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| 345 | { GA_AWAR_LOWERCASE, "lowercase" }, |
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| 346 | { GA_AWAR_WEIGHT, "weight" }, |
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| 347 | { GA_AWAR_FULL_MINLEN, "fullminlen" }, |
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| 348 | |
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| 349 | // advanced only |
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| 350 | { GA_AWAR_SAI, "sai" }, |
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| 351 | { GA_AWAR_AUTOFILTER, "autofilter" }, |
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| 352 | { GA_AWAR_TURN_CHECK, "turncheck" }, |
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| 353 | { GA_AWAR_REALIGN, "realign" }, |
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| 354 | { GA_AWAR_GAP_PEN, "gappen" }, |
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| 355 | { GA_AWAR_GAP_EXT, "gapext" }, |
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| 356 | { GA_AWAR_MATCH_SCORE, "matchscore" }, |
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| 357 | { GA_AWAR_MISMATCH_SCORE, "mismatchscore" }, |
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| 358 | { GA_AWAR_FS_MIN, "fs_min" }, |
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| 359 | { GA_AWAR_FS_MSC, "fs_minscore" }, |
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| 360 | { GA_AWAR_FS_MAX, "fs_min" }, |
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| 361 | { GA_AWAR_MIN_FULL, "minfull" }, |
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| 362 | { GA_AWAR_KMER_LEN, "kmerlen" }, |
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| 363 | { GA_AWAR_KMER_MM, "kmermm" }, |
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| 364 | { GA_AWAR_MIN_LEN, "refminlen" }, |
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| 365 | { GA_AWAR_GENE_START, "genestart" }, |
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| 366 | { GA_AWAR_GENE_END, "geneend" }, |
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| 367 | { GA_AWAR_FS_COVER_GENE, "fs_covergene" }, |
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| 368 | { GA_AWAR_KMER_NOFAST, "kmernofast" }, |
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| 369 | { GA_AWAR_KMER_NOREL, "kmernorel" }, |
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| 370 | |
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| 371 | // both |
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| 372 | { GA_AWAR_SHOW_DIFF, "showdiff" }, |
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| 373 | { GA_AWAR_COLOR, "color" }, |
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| 374 | { GA_AWAR_SHOW_DIST, "showdist" }, |
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| 375 | |
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| 376 | { NULp, NULp } |
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| 377 | }; |
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| 378 | |
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| 379 | static AW_window_simple* new_sina_simple(AW_root *root, const AlignDataAccess *alignData, bool adv) { |
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| 380 | int closex, closey, startx, starty, winx, winy; |
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| 381 | const int hgap = 10; |
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| 382 | AW_window_simple *aws = new AW_window_simple; |
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| 383 | |
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| 384 | aws->init(root, adv ? "XSINA" : "SINA", "SINA (SILVA Incremental Aligner)"); |
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| 385 | |
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| 386 | aws->button_length(12); |
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| 387 | aws->at(10, 10); |
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| 388 | aws->auto_space(5, 5); |
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| 389 | |
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| 390 | aws->callback(AW_POPDOWN); |
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| 391 | aws->create_button("CLOSE", "CLOSE", "O"); |
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| 392 | aws->get_at_position(&closex, &closey); |
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| 393 | |
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| 394 | aws->at_shift(10, 0); |
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| 395 | aws->label_length(0); |
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| 396 | aws->label("Show advanced options"); |
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| 397 | aws->create_toggle(GA_AWAR_ADVANCED); |
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| 398 | |
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| 399 | bool cbInstalled = false; |
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| 400 | if (!cbInstalled) { |
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| 401 | root->awar(GA_AWAR_ADVANCED)->add_callback(makeRootCallback(show_sina_window, alignData)); |
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| 402 | cbInstalled = true; |
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| 403 | } |
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| 404 | |
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| 405 | aws->at_newline(); |
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| 406 | aws->at_shift(0, hgap); |
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| 407 | aws->label_length(15); |
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| 408 | aws->create_toggle_field(GA_AWAR_TGT, "Align what?", "A"); |
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| 409 | aws->insert_toggle("Current Species:", "C", 0); |
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| 410 | aws->insert_toggle("Selected Species", "S", 1); |
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| 411 | aws->insert_default_toggle("Marked Species", "M", 2); |
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| 412 | aws->update_toggle_field(); |
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| 413 | |
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| 414 | aws->at_shift(0, 3); |
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| 415 | aws->create_input_field(AWAR_SPECIES_NAME, 20); |
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| 416 | |
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| 417 | aws->at_newline(); |
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| 418 | aws->at_shift(0, hgap); |
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| 419 | aws->button_length(24); |
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| 420 | aws->label("PT Server:"); |
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| 421 | awt_create_PTSERVER_selection_button(aws, AWAR_PT_SERVER); |
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| 422 | |
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| 423 | aws->at_newline(); |
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| 424 | aws->label_length(0); |
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| 425 | aws->label("Overhang placement"); |
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| 426 | aws->create_option_menu(GA_AWAR_OVERHANG, true); |
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| 427 | aws->insert_option("keep attached", NULp, "attach"); |
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| 428 | aws->insert_option("move to edge", NULp, "edge"); |
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| 429 | aws->insert_option("remove", NULp, "remove"); |
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| 430 | aws->update_option_menu(); |
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| 431 | |
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| 432 | aws->at_newline(); |
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| 433 | aws->label("Handling of unmappable insertions"); |
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| 434 | aws->create_option_menu(GA_AWAR_INSERT, true); |
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| 435 | aws->insert_option("Shift surrounding bases", NULp, "shift"); |
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| 436 | aws->insert_option("Forbid during DP alignment", NULp, "forbid"); |
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| 437 | aws->insert_option("Delete bases", NULp, "remove"); |
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| 438 | aws->update_option_menu(); |
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| 439 | |
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| 440 | aws->at_newline(); |
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| 441 | aws->label("Character Case"); |
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| 442 | aws->create_option_menu(GA_AWAR_LOWERCASE, true); |
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| 443 | aws->insert_option("Do not modify", NULp, "original"); |
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| 444 | aws->insert_option("Show unaligned bases as lower case", NULp, "unaligned"); |
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| 445 | aws->insert_option("Uppercase all", NULp, "none"); |
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| 446 | aws->update_option_menu(); |
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| 447 | |
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| 448 | aws->at_newline(); |
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| 449 | aws->label("Family conservation weight (0-1)"); |
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| 450 | aws->create_input_field(GA_AWAR_WEIGHT, 3); |
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| 451 | |
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| 452 | aws->at_newline(); |
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| 453 | aws->label("Size of full-length sequences"); |
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| 454 | aws->create_input_field(GA_AWAR_FULL_MINLEN, 5); |
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| 455 | |
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| 456 | if (adv) { |
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| 457 | aws->at_newline(); |
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| 458 | aws->at_shift(0, hgap); |
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| 459 | |
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| 460 | aws->at_newline(); |
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| 461 | |
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| 462 | aws->label("Pos. Var.:"); |
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| 463 | awt_create_SAI_selection_button(GLOBAL_gb_main, aws, GA_AWAR_SAI, makeSaiSelectionlistFilterCallback(filter_posvar_SAI_for_ali, alignData->alignment_name.c_str())); |
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| 464 | |
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| 465 | aws->at_newline(); |
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| 466 | aws->label("Field used for automatic filter selection"); |
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| 467 | aws->create_input_field(GA_AWAR_AUTOFILTER, 20); |
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| 468 | |
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| 469 | aws->label("Turn check"); |
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| 470 | aws->create_toggle(GA_AWAR_TURN_CHECK); |
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| 471 | |
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| 472 | aws->at_newline(); |
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| 473 | aws->label("Realign"); |
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| 474 | aws->create_toggle(GA_AWAR_REALIGN); |
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| 475 | |
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| 476 | /* |
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| 477 | aws->at_newline(); |
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| 478 | aws->label("(Copy and) mark sequences used as reference"); |
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| 479 | aws->create_toggle(GA_AWAR_COPYMARKREF); |
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| 480 | */ |
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| 481 | |
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| 482 | aws->at_newline(); |
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| 483 | aws->at_shift(0, hgap); |
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| 484 | aws->label_length(0); |
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| 485 | |
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| 486 | aws->label("Gap insertion/extension penalties"); |
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| 487 | aws->create_input_field(GA_AWAR_GAP_PEN, 5); |
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| 488 | aws->create_input_field(GA_AWAR_GAP_EXT, 5); |
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| 489 | |
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| 490 | aws->at_newline(); |
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| 491 | aws->label("Match score"); |
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| 492 | aws->create_input_field(GA_AWAR_MATCH_SCORE, 3); |
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| 493 | aws->label("Mismatch score"); |
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| 494 | aws->create_input_field(GA_AWAR_MISMATCH_SCORE, 3); |
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| 495 | |
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| 496 | aws->at_newline(); |
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| 497 | aws->label("Family search min/min_score/max"); |
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| 498 | aws->create_input_field(GA_AWAR_FS_MIN, 3); |
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| 499 | aws->create_input_field(GA_AWAR_FS_MSC, 3); |
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| 500 | aws->create_input_field(GA_AWAR_FS_MAX, 3); |
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| 501 | |
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| 502 | aws->at_newline(); |
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| 503 | aws->label("Minimal number of full length sequences"); |
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| 504 | aws->create_input_field(GA_AWAR_MIN_FULL, 3); |
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| 505 | |
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| 506 | aws->at_newline(); |
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| 507 | aws->label("Family search oligo length / mismatches"); |
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| 508 | aws->create_input_field(GA_AWAR_KMER_LEN, 3); |
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| 509 | aws->create_input_field(GA_AWAR_KMER_MM, 3); |
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| 510 | |
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| 511 | aws->at_newline(); |
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| 512 | aws->label("Minimal reference sequence length"); |
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| 513 | aws->create_input_field(GA_AWAR_MIN_LEN, 5); |
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| 514 | |
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| 515 | aws->at_newline(); |
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| 516 | aws->label("Alignment bounds: start"); |
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| 517 | aws->create_input_field(GA_AWAR_GENE_START, 6); |
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| 518 | aws->label("end"); |
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| 519 | aws->create_input_field(GA_AWAR_GENE_END, 6); |
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| 520 | |
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| 521 | aws->at_newline(); |
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| 522 | aws->label("Number of references required to touch bounds"); |
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| 523 | aws->create_input_field(GA_AWAR_FS_COVER_GENE, 3); |
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| 524 | |
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| 525 | aws->at_newline(); |
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| 526 | aws->label("Disable fast search"); |
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| 527 | aws->create_toggle(GA_AWAR_KMER_NOFAST); |
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| 528 | |
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| 529 | aws->at_newline(); |
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| 530 | aws->label("Score search results by absolute oligo match count"); |
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| 531 | aws->create_toggle(GA_AWAR_KMER_NOREL); |
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| 532 | |
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| 533 | aws->at_newline(); |
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| 534 | aws->label("SINA command"); |
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| 535 | aws->create_input_field(GA_AWAR_CMD, 20); |
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| 536 | |
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| 537 | aws->at_shift(0, hgap); |
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| 538 | } |
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| 539 | |
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| 540 | aws->at_newline(); |
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| 541 | aws->at_shift(0, hgap); |
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| 542 | |
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| 543 | aws->label_length(17); |
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| 544 | aws->label("Protection Level"); |
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| 545 | aws->create_option_menu(GA_AWAR_PROTECTION, true); |
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| 546 | aws->insert_option("0", NULp, 0); |
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| 547 | aws->insert_option("1", NULp, 1); |
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| 548 | aws->insert_option("2", NULp, 2); |
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| 549 | aws->insert_option("3", NULp, 3); |
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| 550 | aws->insert_option("4", NULp, 4); |
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| 551 | aws->insert_option("5", NULp, 5); |
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| 552 | aws->insert_option("6", NULp, 6); |
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| 553 | aws->update_option_menu(); |
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| 554 | |
|---|
| 555 | aws->at_newline(); |
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| 556 | aws->label("Show changed sections of alignment"); |
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| 557 | aws->create_toggle(GA_AWAR_SHOW_DIFF); |
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| 558 | aws->label("color bases"); |
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| 559 | aws->create_toggle(GA_AWAR_COLOR); |
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| 560 | |
|---|
| 561 | aws->at_newline(); |
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| 562 | aws->label("Show statistics"); |
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| 563 | aws->create_toggle(GA_AWAR_SHOW_DIST); |
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| 564 | |
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| 565 | aws->get_window_size(winx, winy); |
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| 566 | aws->get_at_position(&startx, &starty); |
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| 567 | |
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| 568 | aws->button_length(12); |
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| 569 | |
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| 570 | aws->at(winx-closex+5, closey); |
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| 571 | aws->callback(makeHelpCallback("sina_main.hlp")); |
|---|
| 572 | aws->create_button("HELP", "HELP"); |
|---|
| 573 | |
|---|
| 574 | aws->at(winx-closex+5, starty); |
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| 575 | aws->callback(makeWindowCallback(sina_start, alignData)); |
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| 576 | aws->highlight(); |
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| 577 | aws->create_button("Start", "Start", "S"); |
|---|
| 578 | |
|---|
| 579 | aws->at(winx-50, starty-50); |
|---|
| 580 | AWT_insert_config_manager(aws, AW_ROOT_DEFAULT, "sina", sina_config_mapping); |
|---|
| 581 | |
|---|
| 582 | return aws; |
|---|
| 583 | } |
|---|
| 584 | |
|---|
| 585 | void show_sina_window(UNFIXED, const AlignDataAccess *alignData) { |
|---|
| 586 | AW_root *root = AW_root::SINGLETON; |
|---|
| 587 | |
|---|
| 588 | static AW_window_simple *ga_aws = new_sina_simple(root, alignData, false); |
|---|
| 589 | static AW_window_simple *ga_aws_adv = new_sina_simple(root, alignData, true); |
|---|
| 590 | |
|---|
| 591 | if (root->awar(GA_AWAR_ADVANCED)->read_int()) { |
|---|
| 592 | ga_aws_adv->show(); |
|---|
| 593 | ga_aws->hide(); |
|---|
| 594 | } |
|---|
| 595 | else { |
|---|
| 596 | ga_aws->show(); |
|---|
| 597 | ga_aws_adv->hide(); |
|---|
| 598 | } |
|---|
| 599 | } |
|---|
| 600 | |
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