1 | // ============================================================ // |
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2 | // // |
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3 | // File : FilteredExport.cxx // |
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4 | // Purpose : encapsulate SAI-filtered fasta exporter // |
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5 | // // |
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6 | // Coded by Ralf Westram (coder@reallysoft.de) in June 2017 // |
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7 | // http://www.arb-home.de/ // |
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8 | // // |
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9 | // ============================================================ // |
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10 | |
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11 | #include "FilteredExport.h" |
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12 | #include <arbdbt.h> |
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13 | #include <gb_aci.h> |
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14 | #include <arb_progress.h> |
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15 | #include <algorithm> |
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16 | |
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17 | AP_filter *FilterDefinition::make_filter(GBDATA *gb_main, const char *aliName, size_t aliSize) const { |
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18 | /*! generate defined filter |
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19 | * @param aliName name of alignment to filter |
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20 | * @return generated filter or NULp (error is exported in that case) |
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21 | */ |
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22 | GB_ERROR error = NULp; |
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23 | AP_filter *filter = NULp; |
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24 | |
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25 | { |
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26 | GBDATA *gb_sai = GBT_expect_SAI(gb_main, sai_name.c_str()); |
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27 | if (!gb_sai) error = GB_await_error(); |
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28 | else { |
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29 | GBDATA *gb_data = GBT_find_sequence(gb_sai, aliName); |
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30 | if (!gb_data) { |
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31 | error = GBS_global_string("SAI '%s' has no data in alignment '%s'", sai_name.c_str(), aliName); |
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32 | } |
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33 | else { |
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34 | #if defined(ASSERTION_USED) |
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35 | long datasize = GB_read_count(gb_data); // may be less than ali-length! |
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36 | arb_assert(datasize == long(aliSize)); // @@@ write a test failing this assertion (BLOCK and PASS need to handle this differently) |
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37 | #endif |
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38 | |
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39 | char *sai_data = GB_read_as_string(gb_data); |
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40 | if (!sai_data) error = GB_await_error(); |
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41 | else { |
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42 | bool blockChars = (type == BLOCK) != inverse; |
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43 | CharRangeTable crt(characters.c_str()); |
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44 | if (blockChars) { |
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45 | filter = new AP_filter(sai_data, crt.expandedRange(), aliSize); // blocks characters |
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46 | } |
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47 | else { |
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48 | AP_filter inv_filt(sai_data, crt.expandedRange(), aliSize); |
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49 | filter = new AP_filter(NOT, inv_filt); |
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50 | } |
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51 | free(sai_data); |
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52 | } |
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53 | } |
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54 | } |
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55 | } |
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56 | |
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57 | arb_assert(contradicted(filter, error)); |
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58 | if (error) GB_export_error(error); |
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59 | return filter; |
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60 | } |
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61 | |
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62 | FilteredExport::FilteredExport(GBDATA *gb_main_, const char *aliname_, size_t alisize_) : |
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63 | gb_main(gb_main_), |
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64 | aliname(nulldup(aliname_)), |
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65 | alisize(alisize_), |
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66 | accept_missing_data(false), |
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67 | header_ACI(strdup("readdb(name)")), |
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68 | sequence_ACI(NULp), |
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69 | count_table(NULp), |
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70 | minCount(0), |
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71 | filter(alisize), |
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72 | filter_added(false) |
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73 | {} |
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74 | |
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75 | FilteredExport::~FilteredExport() { |
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76 | free(header_ACI); |
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77 | free(sequence_ACI); |
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78 | free(aliname); |
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79 | } |
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80 | |
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81 | GB_ERROR FilteredExport::add_SAI_filter(const FilterDefinition& filterDef) { |
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82 | AP_filter *newFilter = filterDef.make_filter(gb_main, aliname, alisize); |
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83 | if (!newFilter) { |
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84 | return GB_await_error(); |
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85 | } |
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86 | |
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87 | if (!filter_added) { |
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88 | filter = *newFilter; |
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89 | filter_added = true; |
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90 | } |
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91 | else { |
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92 | switch (filterDef.get_type()) { |
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93 | case PASS: filter = AP_filter(filter, OR, *newFilter); break; |
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94 | case BLOCK: filter = AP_filter(filter, AND, *newFilter); break; |
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95 | } |
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96 | } |
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97 | delete newFilter; |
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98 | return NULp; |
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99 | } |
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100 | |
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101 | int FilteredExport::count_bases(const char *seq) const { |
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102 | int count = 0; |
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103 | for (int p = 0; seq[p]; ++p) { |
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104 | count += count_table.isSet(seq[p]); |
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105 | } |
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106 | return count; |
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107 | } |
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108 | |
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109 | char *FilteredExport::get_filtered_sequence(GBDATA *gb_species, const char*& reason) const { |
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110 | /* returns filtered sequence or |
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111 | * NULp (which means "do not export") |
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112 | * - if an error occurred (error is exported only in that case!), |
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113 | * - if species had no data in alignment (and accept_missing_data is true) or |
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114 | * - if filtered sequence does not have min. required base count. |
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115 | * If NULp returned and no error exported -> 'reason' gets set! |
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116 | */ |
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117 | arb_assert(gb_species); |
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118 | arb_assert(!GB_have_error()); |
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119 | |
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120 | reason = NULp; |
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121 | |
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122 | char *seq = NULp; |
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123 | GBDATA *gb_data = GBT_find_sequence(gb_species, aliname); |
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124 | |
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125 | if (!gb_data) { |
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126 | if (GB_have_error()) { |
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127 | GB_export_error(GB_failedTo_error("read sequence of ", GBT_read_name(gb_species), GB_await_error())); |
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128 | } |
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129 | else { |
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130 | if (accept_missing_data) { |
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131 | reason = "has no data"; |
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132 | } |
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133 | else { |
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134 | GB_export_errorf("species '%s' has no data in '%s'", GBT_read_name(gb_species), aliname); |
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135 | } |
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136 | } |
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137 | } |
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138 | else { |
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139 | GB_ERROR error = filter.is_invalid(); |
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140 | if (error) GB_export_error(error); |
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141 | else { |
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142 | const char *cseq = GB_read_char_pntr(gb_data); |
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143 | seq = filter.filter_string(cseq); |
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144 | |
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145 | // check min. requirements: |
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146 | if (minCount>0) { // otherwise check would always succeed |
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147 | int count = count_bases(seq); |
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148 | if (count<minCount) { // too few bases -> do not export |
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149 | freenull(seq); |
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150 | reason = "not enough base-characters left"; |
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151 | } |
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152 | } |
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153 | } |
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154 | } |
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155 | |
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156 | if (seq && sequence_ACI) { |
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157 | char *seq_postprocessed = GB_command_interpreter_in_env(seq, sequence_ACI, GBL_simple_call_env(gb_species)); |
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158 | if (seq_postprocessed) { |
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159 | freeset(seq, seq_postprocessed); |
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160 | } |
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161 | else { |
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162 | char *error = strdup(GB_await_error()); |
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163 | GB_export_errorf("Failed to post-process sequence data of species '%s' (Reason: %s)", GBT_read_name(gb_species), error); |
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164 | free(error); |
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165 | freenull(seq); |
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166 | } |
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167 | } |
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168 | |
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169 | arb_assert(contradicted(seq, GB_have_error() || reason)); |
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170 | arb_assert(implicated(!seq, contradicted(GB_have_error(), reason))); |
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171 | |
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172 | return seq; |
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173 | } |
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174 | |
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175 | char *FilteredExport::get_fasta_header(GBDATA *gb_species) const { |
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176 | return GB_command_interpreter_in_env("", header_ACI, GBL_simple_call_env(gb_species)); |
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177 | } |
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178 | |
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179 | GB_ERROR FilteredExport::write_fasta(FILE *out) { |
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180 | GB_ERROR error = NULp; |
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181 | int exported = 0; |
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182 | int skipped = 0; |
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183 | |
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184 | { |
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185 | arb_progress progress("Write sequence data", GBT_get_species_count(gb_main)); |
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186 | |
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187 | for (GBDATA *gb_species = GBT_first_species(gb_main); |
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188 | gb_species && !error; |
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189 | gb_species = GBT_next_species(gb_species)) |
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190 | { |
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191 | const char *reason; |
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192 | char *filt_seq = get_filtered_sequence(gb_species, reason); |
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193 | |
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194 | if (filt_seq) { |
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195 | ++exported; // count exported species |
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196 | fputc('>', out); |
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197 | { |
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198 | char *header = get_fasta_header(gb_species); |
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199 | if (header) { |
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200 | fputs(header, out); |
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201 | free(header); |
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202 | } |
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203 | else { |
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204 | error = GB_await_error(); |
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205 | } |
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206 | } |
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207 | fputc('\n', out); |
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208 | |
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209 | fputs(filt_seq, out); |
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210 | fputc('\n', out); |
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211 | |
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212 | free(filt_seq); |
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213 | } |
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214 | else { |
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215 | if (reason) { |
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216 | ++skipped; // count skipped |
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217 | fprintf(stderr, "Skipped species '%s' (Reason: %s)\n", GBT_read_name(gb_species), reason); |
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218 | } |
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219 | else { |
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220 | error = GB_await_error(); |
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221 | } |
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222 | } |
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223 | ++progress; |
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224 | } |
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225 | |
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226 | if (error) progress.done(); |
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227 | } |
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228 | |
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229 | if (!error) { |
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230 | if (exported) { |
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231 | fprintf(stderr, "Summary: %i species exported", exported); |
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232 | if (skipped) fprintf(stderr, ", %i species skipped", skipped); |
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233 | fputc('\n', stderr); |
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234 | } |
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235 | else { |
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236 | fprintf(stderr, "Summary: all %i species skipped (warning: generated empty file)\n", skipped); |
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237 | } |
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238 | } |
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239 | |
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240 | return error; |
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241 | } |
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242 | |
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243 | // -------------------------------------------------------------------------------- |
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244 | |
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245 | #ifdef UNIT_TESTS |
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246 | #ifndef TEST_UNIT_H |
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247 | #include <test_unit.h> |
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248 | #endif |
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249 | |
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250 | #define TEST_EXPECT_CRT_DEFINES(arg,expected) TEST_EXPECT_EQUAL(CharRangeTable(arg).expandedRange(), expected) |
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251 | |
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252 | #define ABC "ABCDEFGHIJKLMNOPQRSTUVWXYZ" |
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253 | #define abc "abcdefghijklmnopqrstuvwxyz" |
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254 | |
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255 | void TEST_CharRangeTable() { |
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256 | TEST_EXPECT_CRT_DEFINES("abc", "abc"); |
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257 | TEST_EXPECT_CRT_DEFINES("cba", "abc"); |
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258 | TEST_EXPECT_CRT_DEFINES("c-a", "-ac"); // unwanted reverse range does not expand! |
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259 | TEST_EXPECT_CRT_DEFINES("a-c", "abc"); |
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260 | |
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261 | TEST_EXPECT_CRT_DEFINES("a-db-e", "abcde"); |
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262 | TEST_EXPECT_CRT_DEFINES("a-de-b", "-abcde"); |
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263 | |
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264 | TEST_EXPECT_CRT_DEFINES("-ab", "-ab"); |
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265 | TEST_EXPECT_CRT_DEFINES("a-b", "ab"); |
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266 | TEST_EXPECT_CRT_DEFINES("ab-", "-ab"); |
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267 | |
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268 | TEST_EXPECT_CRT_DEFINES("a-ac-c", "ac"); |
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269 | |
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270 | TEST_EXPECT_CRT_DEFINES("a-zA-Z", ABC abc); |
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271 | |
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272 | // dangerous ranges are not expanded: |
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273 | TEST_EXPECT_CRT_DEFINES(".-=", "-.="); |
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274 | TEST_EXPECT_CRT_DEFINES("=-.", "-.="); |
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275 | |
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276 | TEST_EXPECT_CRT_DEFINES("a-Z", "-Za"); |
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277 | TEST_EXPECT_CRT_DEFINES("A-z", "-Az"); |
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278 | } |
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279 | |
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280 | #undef abc |
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281 | #undef ABC |
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282 | |
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283 | #define TEST_EXPECT_SEQWITHLENGTH__NOERROREXPORTED(create_seqcopy, expected_length) do { \ |
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284 | char *seqcopy; \ |
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285 | TEST_EXPECT_RESULT__NOERROREXPORTED(seqcopy = create_seqcopy); \ |
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286 | TEST_EXPECT_EQUAL(strlen(seqcopy), expected_length); \ |
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287 | free(seqcopy); \ |
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288 | } while(0) |
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289 | |
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290 | void TEST_FilteredExport() { |
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291 | // see also ../../TOOLS/arb_test.cxx@SAI_FILTERED_EXPORT_TESTS |
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292 | |
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293 | GB_shell shell; |
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294 | GBDATA *gb_main = GB_open("TEST_prot_tiny.arb", "r"); // ../../UNIT_TESTER/run/TEST_prot_tiny.arb |
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295 | |
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296 | // only "CytLyti6" has data in 'ali_dna_incomplete' |
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297 | |
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298 | { |
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299 | char *ali_name = NULp; |
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300 | size_t ali_size = 0; |
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301 | |
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302 | { |
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303 | GB_transaction ta(gb_main); |
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304 | ali_name = GBT_get_default_alignment(gb_main); |
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305 | ali_size = GBT_get_alignment_len(gb_main, ali_name); |
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306 | } |
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307 | |
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308 | { |
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309 | FilteredExport exporter(gb_main, ali_name, ali_size); |
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310 | FilteredExport exporter_incomplete(gb_main, "ali_dna_incomplete", ali_size); |
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311 | |
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312 | { |
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313 | GB_transaction ta(gb_main); |
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314 | |
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315 | GBDATA *gb_spec1 = GBT_find_species(gb_main, "StrRamo3"); // ~ 60 AA |
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316 | GBDATA *gb_spec2 = GBT_find_species(gb_main, "MucRacem"); // more AA |
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317 | GBDATA *gb_spec3 = GBT_find_species(gb_main, "BctFra12"); |
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318 | GBDATA *gb_spec4 = GBT_find_species(gb_main, "CytLyti6"); |
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319 | |
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320 | TEST_REJECT_NULL(gb_spec1); |
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321 | TEST_REJECT_NULL(gb_spec2); |
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322 | TEST_REJECT_NULL(gb_spec3); |
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323 | TEST_REJECT_NULL(gb_spec4); |
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324 | |
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325 | const char *reason; |
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326 | TEST_EXPECT_SEQWITHLENGTH__NOERROREXPORTED(exporter.get_filtered_sequence(gb_spec1, reason), ali_size); |
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327 | TEST_EXPECT_SEQWITHLENGTH__NOERROREXPORTED(exporter.get_filtered_sequence(gb_spec2, reason), ali_size); |
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328 | |
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329 | TEST_EXPECT_NORESULT__ERROREXPORTED_CONTAINS(exporter_incomplete.get_filtered_sequence(gb_spec3, reason), "has no data in 'ali_dna_incomplete'"); |
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330 | TEST_EXPECT_NULL(reason); |
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331 | |
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332 | exporter_incomplete.do_accept_missing_data(); // changes state of exporter_incomplete! |
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333 | |
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334 | TEST_EXPECT_NORESULT__NOERROREXPORTED(exporter_incomplete.get_filtered_sequence(gb_spec3, reason)); |
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335 | TEST_EXPECT_EQUAL(reason, "has no data"); |
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336 | |
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337 | TEST_EXPECT_SEQWITHLENGTH__NOERROREXPORTED(exporter.get_filtered_sequence(gb_spec4, reason), ali_size); |
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338 | |
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339 | // test header-generation: |
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340 | TEST_EXPECT_EQUAL_STRINGCOPY__NOERROREXPORTED(exporter.get_fasta_header(gb_spec1), "StrRamo3"); |
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341 | TEST_EXPECT_EQUAL_STRINGCOPY__NOERROREXPORTED(exporter.get_fasta_header(gb_spec2), "MucRacem"); |
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342 | |
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343 | exporter.set_header_ACI("readdb(name);\",\";readdb(full_name)"); // use real ACI for header |
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344 | TEST_EXPECT_EQUAL_STRINGCOPY__NOERROREXPORTED(exporter.get_fasta_header(gb_spec3), "BctFra12,Bacteroides fragilis"); |
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345 | |
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346 | exporter.set_header_ACI("readdb(name);\",\";readdb(ali_dna);\",\";readdb(nosuchfield);\",\";readdb(full_name)"); // wrong accepted use (try to read a container and a unknown field) |
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347 | TEST_EXPECT_EQUAL_STRINGCOPY__NOERROREXPORTED(exporter.get_fasta_header(gb_spec4), "CytLyti6,,,Cytophaga lytica"); // both produce empty output |
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348 | |
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349 | exporter.set_header_ACI("readdb(name);\",\";bugme"); // wrong rejected use (unknown command) |
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350 | TEST_EXPECT_NORESULT__ERROREXPORTED_CONTAINS(exporter.get_fasta_header(gb_spec4), "Unknown command 'bugme'"); // aborts with error |
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351 | |
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352 | // test sequences are skipped if to few bases remain: |
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353 | exporter.set_required_baseCount("ACGT", 185); // (bit more than 3*60) |
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354 | TEST_EXPECT_NORESULT__NOERROREXPORTED(exporter.get_filtered_sequence(gb_spec1, reason)); |
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355 | TEST_EXPECT_EQUAL(reason, "not enough base-characters left"); |
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356 | exporter.reset_required_baseCount(); |
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357 | |
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358 | // test FilterDefinition |
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359 | FilterDefinition pvp_blck_variab("POS_VAR_BY_PARSIMONY", BLOCK, true, "-=.0123"); |
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360 | FilterDefinition pvp_pass_variab("POS_VAR_BY_PARSIMONY", PASS, true, "-=.0123"); |
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361 | FilterDefinition pvp_blck_cnsrvd("POS_VAR_BY_PARSIMONY", BLOCK, false, "-=.012345"); |
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362 | FilterDefinition pvp_pass_cnsrvd("POS_VAR_BY_PARSIMONY", PASS , false, "-=.012345"); |
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363 | |
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364 | { |
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365 | AP_filter *filt_blck_variab; |
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366 | AP_filter *filt_pass_variab; |
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367 | AP_filter *filt_blck_cnsrvd; |
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368 | AP_filter *filt_pass_cnsrvd; |
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369 | |
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370 | TEST_EXPECT_RESULT__NOERROREXPORTED(filt_blck_variab = pvp_blck_variab.make_filter(gb_main, ali_name, ali_size)); |
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371 | TEST_EXPECT_RESULT__NOERROREXPORTED(filt_pass_variab = pvp_pass_variab.make_filter(gb_main, ali_name, ali_size)); |
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372 | TEST_EXPECT_RESULT__NOERROREXPORTED(filt_blck_cnsrvd = pvp_blck_cnsrvd.make_filter(gb_main, ali_name, ali_size)); |
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373 | TEST_EXPECT_RESULT__NOERROREXPORTED(filt_pass_cnsrvd = pvp_pass_cnsrvd.make_filter(gb_main, ali_name, ali_size)); |
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374 | |
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375 | TEST_EXPECT_EQUAL(filt_blck_variab->get_length(), ali_size); |
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376 | TEST_EXPECT_EQUAL(filt_pass_variab->get_length(), ali_size); |
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377 | TEST_EXPECT_EQUAL(filt_blck_cnsrvd->get_length(), ali_size); |
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378 | TEST_EXPECT_EQUAL(filt_pass_cnsrvd->get_length(), ali_size); |
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379 | |
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380 | TEST_EXPECT_EQUAL(filt_blck_variab->get_filtered_length(), 135); |
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381 | TEST_EXPECT_EQUAL(filt_pass_variab->get_filtered_length(), ali_size-135); |
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382 | TEST_EXPECT_EQUAL(filt_blck_cnsrvd->get_filtered_length(), ali_size-45); |
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383 | TEST_EXPECT_EQUAL(filt_pass_cnsrvd->get_filtered_length(), 45); |
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384 | |
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385 | delete filt_pass_cnsrvd; |
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386 | delete filt_blck_cnsrvd; |
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387 | delete filt_pass_variab; |
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388 | delete filt_blck_variab; |
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389 | } |
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390 | |
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391 | // test get_filtered_sequence with real filters: |
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392 | TEST_EXPECT_NO_ERROR(exporter.add_SAI_filter(pvp_blck_variab)); |
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393 | TEST_EXPECT_EQUAL_STRINGCOPY__NOERROREXPORTED(exporter.get_filtered_sequence(gb_spec1, reason), "TCCACGCACCAAGTCCT--GGACTTTG--GACCGGGTCCCTGACG-ATG-CCGGGGACG---GACTGG--TC--GGGCCGCT-ACGG---CGCTCGGCCGCG-------GCCG-CTGGG----CCGCCGT....."); |
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394 | |
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395 | exporter.clear_SAI_filters(); |
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396 | |
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397 | TEST_EXPECT_NO_ERROR(exporter.add_SAI_filter(pvp_pass_cnsrvd)); |
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398 | TEST_EXPECT_EQUAL_STRINGCOPY__NOERROREXPORTED(exporter.get_filtered_sequence(gb_spec1, reason), "TCACCAAGTTGGGTCGACCGGGAATGGGCCAGCCCGCGCTGGCCC"); |
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399 | |
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400 | exporter.clear_SAI_filters(); |
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401 | |
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402 | TEST_EXPECT_NO_ERROR(exporter.add_SAI_filter(pvp_blck_cnsrvd)); |
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403 | TEST_EXPECT_NO_ERROR(exporter.add_SAI_filter(pvp_blck_variab)); |
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404 | TEST_EXPECT_EQUAL_STRINGCOPY__NOERROREXPORTED(exporter.get_filtered_sequence(gb_spec1, reason), "CGCACCC--ACTTTG--GACCGGCCTCG-ATG-GCG---GCG--TC--GCGT-CG---CGTCGG-------GCG-CG----GCGT....."); |
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405 | |
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406 | // test sequence post-processing |
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407 | exporter.set_sequence_ACI(":.=-"); // convert dots to hyphens (using SRT) |
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408 | TEST_EXPECT_EQUAL_STRINGCOPY__NOERROREXPORTED(exporter.get_filtered_sequence(gb_spec1, reason), "CGCACCC--ACTTTG--GACCGGCCTCG-ATG-GCG---GCG--TC--GCGT-CG---CGTCGG-------GCG-CG----GCGT-----"); |
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409 | |
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410 | exporter.set_sequence_ACI(":bad"); // malformed expression |
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411 | TEST_EXPECT_NORESULT__ERROREXPORTED_CONTAINS(exporter.get_filtered_sequence(gb_spec1, reason), "SRT ERROR: no '=' found in command 'bad'"); |
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412 | } |
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413 | } |
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414 | free(ali_name); |
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415 | } |
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416 | GB_close(gb_main); |
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417 | } |
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418 | |
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419 | #endif // UNIT_TESTS |
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420 | |
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421 | // -------------------------------------------------------------------------------- |
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422 | |
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423 | |
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