1 | // ================================================================ // |
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2 | // // |
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3 | // File : Importer.cxx // |
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4 | // Purpose : Genome importer core // |
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5 | // // |
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6 | // Coded by Ralf Westram (coder@reallysoft.de) in November 2006 // |
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7 | // Institute of Microbiology (Technical University Munich) // |
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8 | // http://www.arb-home.de/ // |
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9 | // // |
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10 | // ================================================================ // |
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11 | |
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12 | #include "tools.h" |
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13 | #include "DBwriter.h" |
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14 | #include <arbdb.h> |
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15 | #include <arb_stdstr.h> |
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16 | |
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17 | using namespace std; |
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18 | |
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19 | // -------------------------------------------------------------------------------- |
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20 | |
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21 | static bool is_escaped(const string& str, size_t pos) { |
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22 | // returns true, if position 'pos' in string 'str' is escaped by '\\' |
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23 | |
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24 | bool escaped = false; |
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25 | if (pos != 0) { // pos 0 can't be escaped |
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26 | if (str[pos-1] == '\\') { // is an escape before pos ? |
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27 | escaped = !is_escaped(str, pos-1); // pos is escaped, if the escape isn't! |
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28 | } |
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29 | } |
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30 | return escaped; |
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31 | } |
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32 | |
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33 | FeatureLine::FeatureLine(const string& line) { |
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34 | // start parsing at position 5 |
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35 | string::size_type first_char = line.find_first_not_of(' ', 5); |
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36 | |
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37 | orgLine = line; |
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38 | |
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39 | if (first_char == 5) { // feature start |
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40 | string::size_type behind_name = line.find_first_of(' ', first_char); |
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41 | string::size_type rest_start = line.find_first_not_of(' ', behind_name); |
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42 | |
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43 | if (rest_start == string::npos) { |
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44 | if (behind_name == string::npos) throw "Expected space behind feature name"; |
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45 | throw "Expected some content behind feature name"; |
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46 | } |
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47 | |
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48 | name = line.substr(first_char, behind_name-first_char); |
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49 | rest = line.substr(rest_start); |
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50 | type = FL_START; |
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51 | } |
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52 | else if (first_char >= 21) { // not feature start |
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53 | if (first_char == 21 && line[first_char] == '/') { // qualifier start |
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54 | string::size_type equal_pos = line.find_first_of('=', first_char); |
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55 | if (equal_pos == string::npos) { |
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56 | // qualifier w/o data (i.e. "/pseudo") |
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57 | name = line.substr(first_char+1); |
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58 | rest = "true"; |
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59 | type = FL_QUALIFIER_NODATA; |
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60 | } |
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61 | else { |
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62 | name = line.substr(first_char+1, equal_pos-first_char-1); |
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63 | rest = line.substr(equal_pos+1); |
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64 | |
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65 | if (rest[0] == '"') { |
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66 | size_t rlen = rest.length(); |
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67 | |
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68 | if (rlen == 1) { // special case: only one open quote behind qualifier |
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69 | type = FL_QUALIFIER_QUOTE_OPENED; |
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70 | } |
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71 | else if (rest[rlen-1] == '"' && !is_escaped(rest, rlen-1)) { // closing non-escaped quote at eol |
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72 | type = FL_QUALIFIER_QUOTED; |
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73 | } |
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74 | else { |
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75 | type = FL_QUALIFIER_QUOTE_OPENED; |
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76 | } |
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77 | } |
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78 | else { |
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79 | type = FL_QUALIFIER; |
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80 | } |
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81 | } |
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82 | } |
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83 | else { // continued line |
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84 | interpret_as_continued_line(); |
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85 | } |
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86 | } |
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87 | else { |
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88 | if (first_char == string::npos) { |
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89 | throw "Expected feature line, found empty line"; |
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90 | } |
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91 | throw GBS_global_string("Expected feature line (first char at pos=%zu unexpected)", first_char); |
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92 | } |
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93 | } |
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94 | |
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95 | void FeatureLine::interpret_as_continued_line() { |
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96 | rest = orgLine.substr(21); |
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97 | if (rest[rest.length()-1] == '"') { |
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98 | type = FL_CONTINUED_QUOTE_CLOSED; |
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99 | } |
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100 | else { |
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101 | type = FL_CONTINUED; |
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102 | } |
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103 | } |
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104 | |
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105 | bool FeatureLine::reinterpret_as_continued_line() { |
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106 | bool ok = false; |
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107 | |
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108 | if (type == FL_QUALIFIER || type == FL_QUALIFIER_NODATA) { |
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109 | string::size_type first_char = orgLine.find_first_not_of(' ', 5); |
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110 | if (first_char >= 21) { |
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111 | interpret_as_continued_line(); |
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112 | ok = true; |
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113 | } |
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114 | } |
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115 | |
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116 | return ok; |
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117 | } |
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118 | |
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119 | // -------------------------------------------------------------------------------- |
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120 | |
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121 | Importer::Importer(LineReader& Flatfile, DBwriter& DB_writer, const MetaTag *meta_description) |
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122 | : db_writer(DB_writer), |
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123 | flatfile(Flatfile), |
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124 | tagTranslator(meta_description), |
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125 | expectedSeqLength(-1) |
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126 | {} |
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127 | |
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128 | void Importer::warning(const char *msg) { |
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129 | warnings.push_back(msg); |
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130 | } |
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131 | |
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132 | FeatureLinePtr Importer::getFeatureTableLine() { |
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133 | FeatureLinePtr fline; |
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134 | |
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135 | if (pushedFeatureLines.empty()) { // nothing on stack -> read new |
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136 | string line; |
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137 | if (readFeatureTableLine(line)) fline = new FeatureLine(line); |
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138 | } |
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139 | else { |
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140 | fline = pushedFeatureLines.back(); |
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141 | pushedFeatureLines.pop_back(); |
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142 | } |
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143 | return fline; |
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144 | } |
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145 | |
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146 | FeatureLinePtr Importer::getUnwrappedFeatureTableLine() { |
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147 | FeatureLinePtr fline = getFeatureTableLine(); |
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148 | if (!fline.isNull()) { |
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149 | if (fline->type & FL_META_CONTINUED) throw "Expected start of feature or qualifier"; |
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150 | |
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151 | if (0 == (fline->type & (FL_QUALIFIER_NODATA|FL_QUALIFIER_QUOTED))) { |
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152 | // qualifier/featurestart may be wrapped |
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153 | FeatureLinePtr next_fline = getFeatureTableLine(); |
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154 | |
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155 | while (!next_fline.isNull() && |
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156 | fline->type != FL_QUALIFIER_QUOTED) // already seen closing quote |
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157 | { |
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158 | if ((next_fline->type&FL_META_CONTINUED) == 0) { |
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159 | // special case: a wrapped line of a quoted qualifier may start with /xxx |
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160 | // (in that case it is misinterpreted as qualifier start) |
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161 | if (fline->type == FL_QUALIFIER_QUOTE_OPENED) { |
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162 | if (!next_fline->reinterpret_as_continued_line()) { |
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163 | throw "did not see end of quoted qualifier (instead found next qualifiert)"; |
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164 | } |
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165 | gi_assert(next_fline->type & FL_META_CONTINUED); |
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166 | } |
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167 | else { |
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168 | break; |
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169 | } |
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170 | } |
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171 | |
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172 | if (next_fline->type == FL_CONTINUED_QUOTE_CLOSED) { |
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173 | if (fline->type != FL_QUALIFIER_QUOTE_OPENED) throw "Unexpected closing quote"; |
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174 | fline->type = FL_QUALIFIER_QUOTED; |
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175 | } |
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176 | else { |
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177 | gi_assert(next_fline->type == FL_CONTINUED); |
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178 | gi_assert(fline->type == FL_START || fline->type == FL_QUALIFIER || fline->type == FL_QUALIFIER_QUOTE_OPENED); |
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179 | } |
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180 | |
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181 | fline->rest.append(next_fline->rest); |
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182 | next_fline = getFeatureTableLine(); |
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183 | } |
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184 | |
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185 | if (!next_fline.isNull()) backFeatureTableLine(next_fline); |
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186 | } |
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187 | } |
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188 | return fline; |
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189 | } |
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190 | |
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191 | FeaturePtr Importer::parseFeature() { |
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192 | FeaturePtr feature; |
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193 | FeatureLinePtr fline = getUnwrappedFeatureTableLine(); |
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194 | |
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195 | if (!fline.isNull()) { // found a feature table line |
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196 | if (fline->type != FL_START) throw "Expected feature start"; |
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197 | |
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198 | feature = new Feature(fline->name, fline->rest); |
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199 | |
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200 | fline = getUnwrappedFeatureTableLine(); |
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201 | while (!fline.isNull() && (fline->type & FL_META_QUALIFIER)) { |
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202 | feature->addQualifiedEntry(fline->name, fline->rest); |
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203 | fline = getUnwrappedFeatureTableLine(); |
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204 | } |
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205 | if (!fline.isNull()) backFeatureTableLine(fline); |
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206 | } |
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207 | |
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208 | return feature; |
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209 | } |
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210 | |
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211 | void Importer::parseFeatureTable() { |
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212 | FeaturePtr feature = parseFeature(); |
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213 | |
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214 | while (!feature.isNull()) { |
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215 | feature->expectLocationInSequence(expectedSeqLength); |
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216 | feature->fixEmptyQualifiers(); |
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217 | db_writer.writeFeature(*feature, expectedSeqLength); |
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218 | feature = parseFeature(); |
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219 | } |
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220 | } |
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221 | |
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222 | void Importer::show_warnings(const string& import_of_what) { |
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223 | if (!warnings.empty()) { |
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224 | const char *what = import_of_what.c_str(); |
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225 | stringVectorCRIter e = warnings.rend(); |
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226 | for (stringVectorCRIter i = warnings.rbegin(); i != e; ++i) { |
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227 | GB_warningf("Warning: %s: %s", what, i->c_str()); |
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228 | } |
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229 | warnings.clear(); |
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230 | } |
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231 | } |
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232 | |
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233 | |
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234 | void Importer::import() { |
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235 | try { |
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236 | string line; |
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237 | while (flatfile.getLine(line)) { |
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238 | if (!line.empty()) { // silently skip empty lines before or after section |
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239 | flatfile.backLine(line); |
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240 | |
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241 | // cleanup from import of previous section |
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242 | gi_assert(pushedFeatureLines.empty()); // oops - somehow forgot a feature |
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243 | pushedFeatureLines.clear(); |
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244 | warnings.clear(); |
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245 | |
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246 | expectedSeqLength = 0; // reset expected seq. length |
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247 | import_section(); |
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248 | |
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249 | gi_assert(warnings.empty()); |
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250 | gi_assert(pushedFeatureLines.empty()); // oops - somehow forgot a feature |
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251 | } |
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252 | } |
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253 | } |
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254 | catch (const DBerror& err) { throw err.getMessage(); } |
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255 | catch (const string& err) { throw flatfile.lineError(err); } |
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256 | catch (const char *err) { throw flatfile.lineError(err); } |
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257 | } |
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258 | |
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259 | void Importer::check_base_counters(const SequenceBuffer& seqData, const BaseCounter *headerCount) { |
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260 | const BaseCounter& baseCounter = seqData.getBaseCounter(); |
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261 | if (baseCounter.getCount(BC_ALL)<1) { |
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262 | warning("Sequence data is empty (only metadata found)."); |
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263 | } |
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264 | if (!headerCount) { |
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265 | gi_assert(dynamic_cast<GenebankImporter*>(this) != NULp); // this case shall only happen with genebank files. |
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266 | warning("No 'BASE COUNT' found. Base counts have not been validated."); |
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267 | } |
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268 | else { |
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269 | headerCount->expectEqual(baseCounter); |
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270 | } |
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271 | } |
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272 | |
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273 | // -------------------------------------------------------------------------------- |
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274 | // Meta information definitions |
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275 | // |
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276 | // |
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277 | // [ please keep the list of common entries in |
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278 | // ../HELP_SOURCE/source/sp_info.hlp@"Commonly used database entries" |
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279 | // up to date! ] |
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280 | |
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281 | static MetaTag genebank_meta_description[] = { |
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282 | { "LOCUS", "org_locus", MT_HEADER }, |
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283 | |
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284 | { "REFERENCE", "", MT_REF_START }, |
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285 | { " AUTHORS", "author", MT_REF }, |
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286 | { " TITLE", "title", MT_REF }, |
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287 | { " CONSRTM", "refgrp", MT_REF }, |
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288 | { " JOURNAL", "journal", MT_REF }, |
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289 | { " PUBMED", "pubmed_id", MT_REF }, |
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290 | { " MEDLINE", "medline_id", MT_REF }, |
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291 | { " REMARK", "refremark", MT_REF }, |
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292 | |
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293 | { "DEFINITION", "definition", MT_BASIC }, |
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294 | { "ACCESSION", "acc", MT_BASIC }, |
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295 | { "VERSION", "version", MT_BASIC }, |
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296 | { "DBLINK", "db_xref", MT_BASIC }, |
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297 | { "KEYWORDS", "keywd", MT_BASIC }, |
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298 | { "SOURCE", "full_name", MT_BASIC }, |
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299 | { " ORGANISM", "tax", MT_BASIC }, |
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300 | { "COMMENT", "comment", MT_BASIC }, |
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301 | { "PROJECT", "projref", MT_BASIC }, |
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302 | |
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303 | { "FEATURES", "", MT_FEATURE_START }, |
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304 | { "CONTIG", "", MT_CONTIG }, |
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305 | { "BASE", "", MT_SEQUENCE_START }, // BASE COUNT (sometimes missing) |
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306 | { "ORIGIN", "", MT_SEQUENCE_START }, // only used if BASE COUNT is missing |
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307 | { "//", "", MT_END }, |
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308 | |
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309 | { "", "", MT_IGNORE }, // End of array |
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310 | }; |
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311 | |
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312 | static MetaTag embl_meta_description[] = { |
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313 | { "ID", "org_id", MT_HEADER }, |
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314 | |
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315 | { "RN", "", MT_REF_START }, |
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316 | { "RA", "author", MT_REF }, |
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317 | { "RC", "auth_comm", MT_REF }, |
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318 | { "RG", "refgrp", MT_REF }, |
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319 | { "RL", "journal", MT_REF }, |
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320 | { "RP", "nuc_rp", MT_REF }, |
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321 | { "RT", "title", MT_REF }, |
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322 | { "RX", "", MT_REF_DBID }, // @@@ extract field 'pubmed_id' ? |
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323 | |
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324 | { "AC", "acc", MT_BASIC }, |
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325 | { "AH", "assembly_header", MT_BASIC }, |
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326 | { "AS", "assembly_info", MT_BASIC }, |
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327 | { "CC", "comment", MT_BASIC }, |
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328 | { "CO", "contig", MT_BASIC }, |
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329 | { "DE", "description", MT_BASIC }, |
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330 | { "DR", "db_xref", MT_BASIC }, |
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331 | { "DT", "date", MT_BASIC }, |
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332 | { "SV", "version", MT_BASIC }, |
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333 | { "KW", "keywd", MT_BASIC }, |
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334 | { "OS", "full_name", MT_BASIC }, |
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335 | { "OC", "tax", MT_BASIC }, |
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336 | { "OG", "organelle", MT_BASIC }, |
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337 | { "PR", "projref", MT_BASIC }, |
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338 | |
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339 | { "FH", "", MT_FEATURE_START }, |
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340 | { "FT", "", MT_FEATURE }, |
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341 | { "SQ", "", MT_SEQUENCE_START }, |
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342 | { "//", "", MT_END }, |
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343 | |
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344 | { "XX", "", MT_IGNORE }, // spacer |
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345 | |
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346 | { "", "", MT_IGNORE }, // End of array |
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347 | }; |
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348 | |
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349 | // -------------------------------------------------------------------------------- |
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350 | |
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351 | |
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352 | GenebankImporter::GenebankImporter(LineReader& Flatfile, DBwriter& DB_writer) |
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353 | : Importer(Flatfile, DB_writer, genebank_meta_description) |
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354 | {} |
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355 | |
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356 | bool GenebankImporter::readFeatureTableLine(string& line) { |
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357 | if (flatfile.getLine(line)) { |
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358 | if (beginsWith(line, " ")) { |
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359 | return true; |
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360 | } |
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361 | flatfile.backLine(line); |
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362 | } |
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363 | return false; |
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364 | } |
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365 | |
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366 | static bool splitGenebankTag(const string& line, string& tag, string& content) { |
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367 | // split a line into tag (incl. preceding spaces) and content |
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368 | // returns true, if line suffices the format requirements (currently never returns false!) |
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369 | // Note: returns tag="" at wrapped lines |
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370 | |
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371 | string::size_type first_non_space = line.find_first_not_of(' '); |
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372 | |
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373 | if (first_non_space >= 12 || // no tag, only content |
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374 | (first_non_space == string::npos && line.length() == 12)) { // same with empty content |
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375 | tag = ""; |
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376 | content = line.substr(12); |
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377 | return true; |
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378 | } |
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379 | |
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380 | gi_assert(first_non_space<12); |
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381 | |
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382 | string::size_type behind_tag = line.find_first_of(' ', first_non_space); |
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383 | if (behind_tag == string::npos) { // only tag w/o spaces behind |
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384 | tag = line; |
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385 | content = ""; |
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386 | return true; |
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387 | } |
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388 | |
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389 | string::size_type content_start = line.find_first_not_of(' ', behind_tag); |
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390 | if (content_start == string::npos) { // line w/o content |
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391 | content = ""; |
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392 | } |
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393 | else { |
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394 | content = line.substr(content_start); |
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395 | } |
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396 | |
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397 | tag = line.substr(0, behind_tag); |
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398 | return true; |
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399 | } |
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400 | |
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401 | static long scanSeqlenFromLOCUS(const string& locusContent) { |
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402 | StringParser parser(locusContent); |
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403 | parser.extractWord(); // id |
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404 | parser.eatSpaces(); |
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405 | |
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406 | long bp = parser.extractNumber(); |
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407 | parser.eatSpaces(); |
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408 | parser.expectContent("bp"); |
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409 | |
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410 | return bp; |
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411 | } |
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412 | |
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413 | void GenebankImporter::import_section() { |
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414 | MetaInfo meta; |
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415 | References refs; |
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416 | |
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417 | const MetaTag *prevTag = NULp; // previously handled tag |
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418 | string prevContent; // previously found content |
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419 | |
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420 | bool seenHeaderLine = false; |
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421 | bool EOS = false; // end of section ? |
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422 | |
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423 | // read header of file |
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424 | while (!EOS) { |
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425 | string line, tag, content; |
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426 | expectLine(line); |
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427 | if (!splitGenebankTag(line, tag, content)) { |
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428 | gi_assert(0); |
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429 | } |
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430 | |
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431 | if (tag.empty()) { // no tag - happens at wrapped lines |
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432 | if (!content.empty()) { // do not append empty 'lines' from wrapped tag-entries. |
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433 | prevContent.append(1, ' '); |
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434 | prevContent.append(content); |
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435 | } |
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436 | } |
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437 | else { // start of new tag |
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438 | const MetaTag *knownTag = findTag(tag); |
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439 | if (!knownTag) throw GBS_global_string("Invalid tag '%s'", tag.c_str()); |
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440 | |
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441 | if (prevTag) { // save previous tag |
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442 | switch (prevTag->type) { |
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443 | case MT_REF: refs.add(prevTag->field, prevContent); break; |
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444 | case MT_BASIC: meta.add(prevTag, prevContent, true); break; |
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445 | case MT_HEADER: |
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446 | meta.add(prevTag, prevContent, true); // save header line |
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447 | expectedSeqLength = scanSeqlenFromLOCUS(prevContent); |
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448 | break; |
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449 | case MT_REF_DBID: // embl only |
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450 | default: gi_assert(0); break; |
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451 | } |
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452 | prevTag = NULp; |
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453 | } |
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454 | |
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455 | switch (knownTag->type) { |
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456 | case MT_HEADER: |
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457 | if (seenHeaderLine) throw GBS_global_string("Multiple occurrences of tag '%s'", tag.c_str()); |
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458 | seenHeaderLine = true; |
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459 | // fall-through |
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460 | case MT_BASIC: |
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461 | case MT_REF: |
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462 | prevTag = knownTag; |
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463 | prevContent = content; |
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464 | break; |
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465 | |
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466 | case MT_REF_START: |
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467 | refs.start(); // start a new reference |
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468 | break; |
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469 | |
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470 | case MT_FEATURE_START: |
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471 | db_writer.createOrganism(flatfile.getFilename(), "NCBI"); |
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472 | parseFeatureTable(); |
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473 | break; |
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474 | |
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475 | case MT_SEQUENCE_START: |
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476 | parseSequence(knownTag->tag, content); |
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477 | EOS = true; // end of section |
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478 | break; |
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479 | |
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480 | case MT_IGNORE: |
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481 | break; |
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482 | |
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483 | case MT_END: |
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484 | EOS = true; |
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485 | break; |
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486 | |
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487 | case MT_CONTIG: |
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488 | throw GBS_global_string("Cannot import files containing CONTIG"); |
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489 | |
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490 | case MT_REF_DBID: // embl only |
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491 | default: |
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492 | gi_assert(0); |
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493 | throw GBS_global_string("Tag '%s' not expected here", knownTag->tag.c_str()); |
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494 | } |
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495 | } |
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496 | } |
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497 | |
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498 | db_writer.finalizeOrganism(meta, refs, *this); |
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499 | show_warnings(meta.getAccessionNumber()); |
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500 | } |
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501 | |
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502 | // -------------------------------------------------------------------------------- |
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503 | |
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504 | |
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505 | EmblImporter::EmblImporter(LineReader& Flatfile, DBwriter& DB_writer) |
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506 | : Importer(Flatfile, DB_writer, embl_meta_description) |
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507 | {} |
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508 | |
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509 | static bool splitEmblTag(const string& line, string& tag, string& content) { |
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510 | // split a line into 2-character tag and content |
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511 | // return true on success (i.e. if line suffices the required format) |
---|
512 | |
---|
513 | if (line.length() == 2) { |
---|
514 | tag = line; |
---|
515 | content = ""; |
---|
516 | } |
---|
517 | else { |
---|
518 | string::size_type spacer = line.find(" "); // separator between tag and content |
---|
519 | if (spacer != 2) return false; // expect spacer at pos 2-4 |
---|
520 | |
---|
521 | tag = line.substr(0, 2); |
---|
522 | content = line.substr(5); |
---|
523 | } |
---|
524 | |
---|
525 | return true; |
---|
526 | } |
---|
527 | |
---|
528 | bool EmblImporter::readFeatureTableLine(string& line) { |
---|
529 | if (flatfile.getLine(line)) { |
---|
530 | if (beginsWith(line, "FT ")) { |
---|
531 | return true; |
---|
532 | } |
---|
533 | flatfile.backLine(line); |
---|
534 | } |
---|
535 | return false; |
---|
536 | } |
---|
537 | |
---|
538 | static long scanSeqlenFromID(const string& idContent) { |
---|
539 | StringParser parser(idContent); |
---|
540 | string lastWord = parser.extractWord(); // eat id |
---|
541 | bool bpseen = false; |
---|
542 | long bp = -1; |
---|
543 | |
---|
544 | while (!bpseen) { |
---|
545 | parser.eatSpaces(); |
---|
546 | string word = parser.extractWord(); |
---|
547 | if (word == "BP.") { |
---|
548 | // basecount is in word before "BP." |
---|
549 | bp = atol(lastWord.c_str()); |
---|
550 | bpseen = true; |
---|
551 | } |
---|
552 | else { |
---|
553 | lastWord = word; |
---|
554 | } |
---|
555 | } |
---|
556 | |
---|
557 | if (bp == -1) throw "Could not parse bp from header"; |
---|
558 | |
---|
559 | return bp; |
---|
560 | } |
---|
561 | |
---|
562 | void EmblImporter::import_section() { |
---|
563 | MetaInfo meta; |
---|
564 | References refs; |
---|
565 | |
---|
566 | const MetaTag *prevTag = NULp; // previously handled tag |
---|
567 | string prevContent; // previously found content |
---|
568 | bool prevAppendNL = false; // append '\n' into multiline tags |
---|
569 | |
---|
570 | bool seenHeaderLine = false; |
---|
571 | bool EOS = false; // end of section ? |
---|
572 | |
---|
573 | // read header of file |
---|
574 | while (!EOS) { |
---|
575 | string line, tag, content; |
---|
576 | expectLine(line); |
---|
577 | if (!splitEmblTag(line, tag, content)) { |
---|
578 | throw "Expected two-character tag at start of line"; |
---|
579 | } |
---|
580 | |
---|
581 | const MetaTag *knownTag = findTag(tag); |
---|
582 | if (!knownTag) throw GBS_global_string("Invalid tag '%s'", tag.c_str()); |
---|
583 | |
---|
584 | if (knownTag == prevTag) { // multiline tag |
---|
585 | if (prevAppendNL) prevContent.append("\n"); // append a newline to make parsing in add_dbid() more easy |
---|
586 | prevContent.append(content); // append w/o space - EMBL flatfiles have spaces at EOL when needed |
---|
587 | } |
---|
588 | else { // start of new tag |
---|
589 | if (prevTag) { // save previous tag |
---|
590 | switch (prevTag->type) { |
---|
591 | case MT_REF: refs.add(prevTag->field, prevContent); break; |
---|
592 | case MT_REF_DBID: refs.add_dbid(prevContent); prevAppendNL = false; break; |
---|
593 | case MT_BASIC: meta.add(prevTag, prevContent, true); break; |
---|
594 | case MT_HEADER: |
---|
595 | meta.add(prevTag, prevContent, true); |
---|
596 | expectedSeqLength = scanSeqlenFromID(prevContent); |
---|
597 | break; |
---|
598 | default: gi_assert(0); break; |
---|
599 | } |
---|
600 | prevTag = NULp; |
---|
601 | } |
---|
602 | |
---|
603 | switch (knownTag->type) { |
---|
604 | case MT_HEADER: |
---|
605 | if (seenHeaderLine) throw GBS_global_string("Multiple occurrences of tag '%s'", tag.c_str()); |
---|
606 | seenHeaderLine = true; |
---|
607 | // fall-through |
---|
608 | case MT_BASIC: |
---|
609 | case MT_REF: |
---|
610 | prevTag = knownTag; |
---|
611 | prevContent = content; |
---|
612 | break; |
---|
613 | |
---|
614 | case MT_REF_DBID: |
---|
615 | prevTag = knownTag; |
---|
616 | prevContent = content; |
---|
617 | prevAppendNL = true; |
---|
618 | break; |
---|
619 | |
---|
620 | case MT_REF_START: |
---|
621 | refs.start(); // start a new reference |
---|
622 | break; |
---|
623 | |
---|
624 | case MT_FEATURE: |
---|
625 | flatfile.backLine(line); |
---|
626 | db_writer.createOrganism(flatfile.getFilename(), "EMBL"); |
---|
627 | parseFeatureTable(); |
---|
628 | break; |
---|
629 | |
---|
630 | case MT_SEQUENCE_START: |
---|
631 | parseSequence(content); |
---|
632 | EOS = true; // end of section |
---|
633 | break; |
---|
634 | |
---|
635 | case MT_FEATURE_START: |
---|
636 | case MT_IGNORE: |
---|
637 | break; |
---|
638 | |
---|
639 | default: |
---|
640 | gi_assert(0); |
---|
641 | throw GBS_global_string("Tag '%s' not expected here", knownTag->tag.c_str()); |
---|
642 | } |
---|
643 | } |
---|
644 | } |
---|
645 | db_writer.finalizeOrganism(meta, refs, *this); |
---|
646 | show_warnings(meta.getAccessionNumber()); |
---|
647 | } |
---|
648 | |
---|
649 | // -------------------------------------------------------------------------------- |
---|
650 | // sequence readers: |
---|
651 | |
---|
652 | inline bool parseCounter(bool expect, BaseCounter& headerCount, StringParser& parser, Base base, const char *word) { |
---|
653 | // parses part of string (e.g. " 6021225 BP;" or " 878196 A;") |
---|
654 | // if 'expect' == true -> throw exception if missing |
---|
655 | // if 'expect' == false -> return false if missing |
---|
656 | |
---|
657 | bool found = false; |
---|
658 | stringCIter start = parser.getPosition(); |
---|
659 | |
---|
660 | parser.expectSpaces(0); |
---|
661 | |
---|
662 | bool seen_number; |
---|
663 | long count = parser.eatNumber(seen_number); |
---|
664 | |
---|
665 | if (seen_number) { |
---|
666 | headerCount.addCount(base, count); |
---|
667 | size_t spaces = parser.eatSpaces(); |
---|
668 | if (spaces>0) { |
---|
669 | size_t len = parser.lookingAt(word); |
---|
670 | if (len>0) { // seen |
---|
671 | parser.advance(len); |
---|
672 | found = true; |
---|
673 | } |
---|
674 | } |
---|
675 | } |
---|
676 | |
---|
677 | if (!found) { |
---|
678 | parser.setPosition(start); // reset position |
---|
679 | if (expect) throw GBS_global_string("Expected counter '### %s', found '%s'", word, parser.rest().c_str()); |
---|
680 | } |
---|
681 | return found; |
---|
682 | } |
---|
683 | |
---|
684 | void GenebankImporter::parseSequence(const string& tag, const string& headerline) { |
---|
685 | SmartPtr<BaseCounter> headerCount; |
---|
686 | |
---|
687 | if (tag == "BASE") { // base count not always present |
---|
688 | // parse headerline : |
---|
689 | headerCount = new BaseCounter("sequence header"); |
---|
690 | { |
---|
691 | StringParser parser(headerline); |
---|
692 | |
---|
693 | parser.expectContent("COUNT"); |
---|
694 | |
---|
695 | parseCounter(true, *headerCount, parser, BC_A, "a"); |
---|
696 | parseCounter(true, *headerCount, parser, BC_C, "c"); |
---|
697 | parseCounter(true, *headerCount, parser, BC_G, "g"); |
---|
698 | parseCounter(true, *headerCount, parser, BC_T, "t"); |
---|
699 | parseCounter(false, *headerCount, parser, BC_OTHER, "others"); // not always present |
---|
700 | |
---|
701 | headerCount->calcOverallCounter(); |
---|
702 | } |
---|
703 | } |
---|
704 | |
---|
705 | // parse sequence data |
---|
706 | size_t est_seq_size = headerCount.isNull() ? 500000 : headerCount->getCount(BC_ALL); |
---|
707 | SequenceBuffer seqData(est_seq_size); |
---|
708 | { |
---|
709 | string line; |
---|
710 | |
---|
711 | if (!headerCount.isNull()) { |
---|
712 | // if BASE COUNT was present, check ORIGIN line |
---|
713 | // otherwise ORIGIN line has already been read |
---|
714 | expectLine(line); |
---|
715 | if (!beginsWith(line, "ORIGIN")) throw "Expected 'ORIGIN'"; |
---|
716 | } |
---|
717 | |
---|
718 | bool eos_seen = false; |
---|
719 | while (!eos_seen) { |
---|
720 | expectLine(line); |
---|
721 | if (beginsWith(line, "//")) { |
---|
722 | eos_seen = true; |
---|
723 | } |
---|
724 | else { |
---|
725 | string data; |
---|
726 | data.reserve(60); |
---|
727 | StringParser parser(line); |
---|
728 | |
---|
729 | parser.eatSpaces(); // not sure whether there really have to be spaces if number has 9 digits or more |
---|
730 | size_t cur_pos = (size_t)parser.extractNumber(); |
---|
731 | size_t datasize = seqData.getBaseCounter().getCount(BC_ALL); |
---|
732 | |
---|
733 | if (cur_pos != (datasize+1)) { |
---|
734 | throw GBS_global_string("Got wrong base position (found=%zu, expected=%zu)", cur_pos, size_t(datasize+1)); |
---|
735 | } |
---|
736 | |
---|
737 | int blocks = 0; |
---|
738 | while (!parser.atEnd() && parser.at() == ' ') { |
---|
739 | parser.expectSpaces(1); |
---|
740 | |
---|
741 | stringCIter start = parser.pos; |
---|
742 | stringCIter end = parser.find(' '); |
---|
743 | |
---|
744 | data.append(start, end); |
---|
745 | blocks++; |
---|
746 | } |
---|
747 | |
---|
748 | if (blocks>6) throw "Found more than 6 parts of sequence data"; |
---|
749 | seqData.addLine(data); |
---|
750 | } |
---|
751 | } |
---|
752 | } |
---|
753 | |
---|
754 | check_base_counters(seqData, headerCount.content()); |
---|
755 | db_writer.writeSequence(seqData); |
---|
756 | } |
---|
757 | |
---|
758 | void EmblImporter::parseSequence(const string& headerline) { |
---|
759 | // parse headerline: |
---|
760 | BaseCounter headerCount("sequence header"); |
---|
761 | { |
---|
762 | StringParser parser(headerline); |
---|
763 | |
---|
764 | parser.expectContent("Sequence"); |
---|
765 | |
---|
766 | parseCounter(true, headerCount, parser, BC_ALL, "BP;"); |
---|
767 | parseCounter(true, headerCount, parser, BC_A, "A;"); |
---|
768 | parseCounter(true, headerCount, parser, BC_C, "C;"); |
---|
769 | parseCounter(true, headerCount, parser, BC_G, "G;"); |
---|
770 | parseCounter(true, headerCount, parser, BC_T, "T;"); |
---|
771 | parseCounter(true, headerCount, parser, BC_OTHER, "other;"); |
---|
772 | |
---|
773 | headerCount.checkOverallCounter(); |
---|
774 | } |
---|
775 | |
---|
776 | // parse sequence data |
---|
777 | SequenceBuffer seqData(headerCount.getCount(BC_ALL)); |
---|
778 | { |
---|
779 | bool eos_seen = false; |
---|
780 | string line; |
---|
781 | |
---|
782 | while (!eos_seen) { |
---|
783 | expectLine(line); |
---|
784 | if (beginsWith(line, "//")) { |
---|
785 | eos_seen = true; |
---|
786 | } |
---|
787 | else { |
---|
788 | string data; |
---|
789 | data.reserve(60); |
---|
790 | StringParser parser(line); |
---|
791 | |
---|
792 | parser.expectSpaces(5, false); |
---|
793 | int blocks = 0; |
---|
794 | while (!parser.atEnd() && isalpha(parser.at())) { |
---|
795 | stringCIter start = parser.pos; |
---|
796 | stringCIter end = parser.find(' '); |
---|
797 | |
---|
798 | data.append(start, end); |
---|
799 | blocks++; |
---|
800 | parser.expectSpaces(1); |
---|
801 | } |
---|
802 | |
---|
803 | if (blocks>6) throw "Found more than 6 parts of sequence data"; |
---|
804 | |
---|
805 | size_t basecount = (size_t)parser.extractNumber(); |
---|
806 | |
---|
807 | seqData.addLine(data); |
---|
808 | size_t datasize = seqData.getBaseCounter().getCount(BC_ALL); |
---|
809 | |
---|
810 | if (basecount != datasize) { |
---|
811 | throw GBS_global_string("Got wrong base counter(found=%zu, expected=%zu)", basecount, datasize); |
---|
812 | } |
---|
813 | } |
---|
814 | } |
---|
815 | } |
---|
816 | |
---|
817 | check_base_counters(seqData, &headerCount); |
---|
818 | db_writer.writeSequence(seqData); |
---|
819 | } |
---|
820 | |
---|