| 1 | # main topics: |
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| 2 | UP arb.hlp |
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| 3 | UP glossary.hlp |
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| 4 | UP pars.hlp |
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| 5 | |
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| 6 | # sub topics: |
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| 7 | SUB pa_add.hlp |
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| 8 | |
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| 9 | # format described in ../help.readme |
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| 10 | |
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| 11 | |
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| 12 | TITLE Add species without optimizing topology |
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| 13 | |
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| 14 | OCCURRENCE ARB_NT/Tree/Add Species to Existing Tree/ARB Parsimony (Quick add marked) |
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| 15 | ARB_PARSIMONY/Tree/Add Species to Tree/Add Marked Species |
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| 16 | ARB_PARSIMONY/Tree/Add Species to Tree/Add Selected Species |
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| 17 | ARB_PARSIMONY/Tree/Add Species to Tree/Remove & Add Marked Species |
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| 18 | |
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| 19 | DESCRIPTION All affected 'species' are positioned according to maximum |
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| 20 | parsimony criteria. The current tree topology will not be optimized |
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| 21 | after insertion. |
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| 22 | |
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| 23 | In case of 'Add Selected Species' the |
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| 24 | currently LINK{selected.hlp} gets |
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| 25 | inserted. The other functions always work on all 'marked species'. |
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| 26 | |
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| 27 | In case of 'Remove & Add Marked Species' any marked species are first |
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| 28 | removed from the tree and then inserted again. |
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| 29 | The other functions only add those marked species which are not |
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| 30 | already present in the tree. |
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| 31 | |
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| 32 | |
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| 33 | SECTION Full length vs partial sequence data |
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| 34 | |
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| 35 | This function only works correct when adding full-length sequences (FLS), |
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| 36 | where full-length here applies to the resulting positions after |
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| 37 | applying the selected alignment filter(s). |
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| 38 | |
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| 39 | Any partially missing sequence data will be considered an deletion and |
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| 40 | adds extra distance between full- and partial sequences. |
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| 41 | |
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| 42 | One common effect of doing so, is that partial sequences often group together |
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| 43 | although they obviously are not (parts of) near relatives, just because they |
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| 44 | do not share (enough) alignment regions. |
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| 45 | |
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| 46 | To avoid that problem |
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| 47 | - do not use partial sequence data (recommended) or |
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| 48 | - (if you really have to) declare and handle partial sequence |
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| 49 | data as such, by using LINK{pa_partial.hlp}. |
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| 50 | |
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| 51 | |
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| 52 | SECTION Tree degeneration |
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| 53 | |
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| 54 | As no topology optimization takes place here, the tree will tend to |
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| 55 | degenerate while you add more and more species, i.e. the tree will |
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| 56 | continuously diverge from an optimized topology build upon the same |
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| 57 | set of species. |
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| 58 | |
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| 59 | Nevertheless this function has proven useful to determine the |
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| 60 | phylogenetic position of new/custom sequences in an optimized tree. |
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| 61 | But you should always be aware that, the more often you add sequences |
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| 62 | this way, the worser your topology will get. |
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| 63 | |
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| 64 | Probably you may want to optimize your tree from time |
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| 65 | to time. See LINK{pa_optimizer.hlp} for howto. |
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| 66 | |
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| 67 | |
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| 68 | SECTION Insertion order |
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| 69 | |
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| 70 | Until arb-6.0.x species with more base positions were added before |
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| 71 | such with less bp. |
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| 72 | |
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| 73 | Starting with arb-7.0 all species are added independently, i.e. |
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| 74 | there is no explicit insertion order (see exception below). |
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| 75 | The performance has been improved dramatically, especially |
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| 76 | when adding many species and/or when adding into big trees. |
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| 77 | |
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| 78 | If multiple species are added to the same position in the tree, |
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| 79 | they form a subtree which will be optimized afterwards. |
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| 80 | |
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| 81 | Exception: |
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| 82 | |
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| 83 | If the number of added species exceeds 50% of the size of |
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| 84 | the tree (e.g. if adding more than 1000 species to a tree |
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| 85 | containing 2000 sp.), the species will be devided into |
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| 86 | multiple sets and then these sets will be inserted one after |
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| 87 | each other. |
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| 88 | Longer sequences will be added earlier in this case. |
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| 89 | |
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| 90 | |
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| 91 | NOTES The current parsimony value (see LINK{pa_value.hlp}) is |
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| 92 | displayed after the 'Current Par' prompt in the upper part of |
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| 93 | the 'ARB_PARSIMONY' window and the 'Message' window (pops up |
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| 94 | while calculating). |
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| 95 | |
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| 96 | EXAMPLES None |
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| 97 | |
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| 98 | WARNINGS The phylogenetic information conferred by the new sequence(s) is |
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| 99 | not used for global tree optimization. |
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| 100 | |
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| 101 | BUGS No bugs known |
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