| 1 | # main topics: |
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| 2 | UP arb.hlp |
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| 3 | UP glossary.hlp |
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| 4 | UP rna3d_general.hlp |
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| 5 | |
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| 6 | # sub topics: |
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| 7 | #SUB subtopic.hlp |
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| 8 | |
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| 9 | # format described in ../help.readme |
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| 10 | |
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| 11 | |
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| 12 | TITLE Mapping Secondary Structural Information |
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| 13 | |
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| 14 | OCCURRENCE In ARB primary structure editor (ARB_EDIT4) -> RNA3D |
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| 15 | |
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| 16 | DESCRIPTION |
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| 17 | |
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| 18 | Secondary and tertiary structure interactions of the well established comparative |
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| 19 | structure models of rRNA which are used in SECEDIT to generate 2D structure models are |
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| 20 | fitted to the three-dimensional structure of E. coli master sequence. |
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| 21 | |
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| 22 | DISPLAY HELICES: |
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| 23 | |
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| 24 | Enabling this check box maps the secondary structural motifs (loops, helices |
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| 25 | and bulges) onto the molecule. Secondary structural information is according |
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| 26 | to the comparative models of rRNA used in primary and secondary structure |
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| 27 | editors. |
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| 28 | |
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| 29 | HELIX SKELETON: |
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| 30 | |
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| 31 | Enabling will draw a skeleton of secondary structure mask. By setting a grey |
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| 32 | or light color you can achieve transparent mask avoiding any interference with |
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| 33 | other information overlays. |
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| 34 | |
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| 35 | DISPLAY MID-HELIX: |
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| 36 | |
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| 37 | This displays middle point of the helices. |
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| 38 | |
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| 39 | DISPLAY HELIX NUMBER: |
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| 40 | |
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| 41 | Checking this box will display the corresponding helix numbers in the rRNA 3D |
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| 42 | structure. Helix numbers are according to ARB numbering scheme. The small |
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| 43 | subunit (16S) rRNA of E.coli contains 50 helices which are numbered from 1 to |
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| 44 | 50. |
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| 45 | |
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| 46 | DISPLAY NUMBER OF HELICES: |
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| 47 | |
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| 48 | Using this you can set the number of helices you would like to be displayed in |
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| 49 | the 3D molecule. This feature is very helpful to thoroughly examine the |
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| 50 | specific helices in the structure. |
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| 51 | |
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| 52 | HELIX SIZE: |
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| 53 | |
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| 54 | Thickness or the size of the helices can be set by specifying the desired |
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| 55 | value in this box. |
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| 56 | |
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| 57 | DISPLAY TERTIARY INTERACTIONS: |
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| 58 | |
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| 59 | The tertiary interactions observed in small subunit rRNA can be displayed in |
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| 60 | the three-dimensional conformations of small subunit rRNA by enabling this |
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| 61 | check box. The tertiary information data is from Gautheret et al. |
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| 62 | |
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| 63 | Color settings related to helix, skeleton, mid-helix, helix number and tertiary |
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| 64 | interactions can be changed using âColor Settingsâ of the main RNA3D window. |
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| 65 | |
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| 66 | NOTES |
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| 67 | |
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| 68 | Gautheret D, Damberger SH, Gutell RR: Identification of base-triples in RNA using |
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| 69 | comparative sequence analysis. J Mol Biol 1995, 248: 27-43. |
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| 70 | |
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| 71 | |
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| 72 | EXAMPLES None |
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| 73 | |
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| 74 | WARNINGS None |
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| 75 | |
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| 76 | BUGS No bugs known |
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