1 | // =============================================================== // |
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2 | // // |
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3 | // File : seq_search.cxx // |
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4 | // Purpose : // |
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5 | // // |
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6 | // Coded by Ralf Westram (coder@reallysoft.de) // |
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7 | // Institute of Microbiology (Technical University Munich) // |
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8 | // http://www.arb-home.de/ // |
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9 | // // |
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10 | // =============================================================== // |
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11 | |
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12 | #include "seq_search.hxx" |
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13 | |
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14 | #include <arbdbt.h> |
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15 | #include <cstdarg> |
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16 | #include <climits> |
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17 | #include <cctype> |
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18 | |
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19 | #define MESSAGE_BUFFERSIZE 300 |
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20 | |
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21 | #if defined(DEBUG) |
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22 | inline int check_equal(int o, int n) { |
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23 | if (o != n) { |
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24 | fprintf(stderr, "o=%i\nn=%i\n", o, n); |
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25 | fflush(stderr); |
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26 | } |
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27 | fa_assert(o == n); |
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28 | return n; |
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29 | } |
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30 | #endif |
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31 | |
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32 | __ATTR__FORMAT(1) static void messagef(const char *format, ...) { |
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33 | va_list argp; |
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34 | char buffer[MESSAGE_BUFFERSIZE]; |
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35 | |
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36 | va_start(argp, format); |
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37 | |
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38 | IF_ASSERTION_USED(int chars =) vsprintf(buffer, format, argp); |
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39 | fa_assert(chars<MESSAGE_BUFFERSIZE); |
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40 | |
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41 | va_end(argp); |
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42 | GB_warning(buffer); |
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43 | } |
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44 | |
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45 | void Dots::append(Dots *neu) { |
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46 | if (Next) Next->append(neu); |
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47 | else Next = neu; |
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48 | } |
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49 | |
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50 | static CharPredicate pred_is_ali_gap(is_ali_gap); |
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51 | |
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52 | CompactedSequence::CompactedSequence(const char *Text, int Length, const char *name, int start_offset) |
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53 | : basepos(Text, Length, pred_is_ali_gap), |
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54 | myName(strdup(name)), |
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55 | myStartOffset(0), // corrected at end of ctor (otherwise calculations below go wrong) |
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56 | dots(NULp) |
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57 | // referred(1) |
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58 | { |
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59 | fa_assert(Length>0); |
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60 | |
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61 | { |
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62 | long firstBase = basepos.first_base_abspos(); |
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63 | long lastBase = basepos.last_base_abspos(); |
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64 | int dotOffset = firstBase + strcspn(Text+firstBase, "."); // skip non-dots |
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65 | |
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66 | while (dotOffset <= lastBase) { |
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67 | fa_assert(Text[dotOffset] == '.'); |
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68 | storeDots(basepos.abs_2_rel(dotOffset)); |
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69 | dotOffset += strspn(Text+dotOffset, "."); // skip dots |
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70 | dotOffset += strcspn(Text+dotOffset, "."); // skip non-dots |
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71 | } |
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72 | } |
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73 | |
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74 | int cLen = length(); |
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75 | myText = new char[cLen+1]; |
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76 | myText[cLen] = 0; |
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77 | |
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78 | gapsBeforePosition = new int[cLen+1]; |
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79 | |
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80 | for (int cPos = 0; cPos<cLen; ++cPos) { |
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81 | int xPos = basepos.rel_2_abs(cPos); |
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82 | myText[cPos] = toupper(Text[xPos]); |
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83 | gapsBeforePosition[cPos] = no_of_gaps_before(cPos); |
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84 | } |
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85 | if (cLen>0) { |
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86 | gapsBeforePosition[cLen] = no_of_gaps_before(cLen); // gaps before end of sequence |
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87 | } |
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88 | |
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89 | myStartOffset += start_offset; |
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90 | } |
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91 | |
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92 | CompactedSequence::~CompactedSequence() { |
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93 | delete [] myText; |
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94 | delete[] gapsBeforePosition; |
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95 | free(myName); |
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96 | delete dots; |
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97 | } |
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98 | |
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99 | FastAlignInsertion::~FastAlignInsertion() { |
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100 | if (myNext) delete myNext; |
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101 | } |
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102 | |
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103 | FastSearchSequence::FastSearchSequence(const CompactedSubSequence& seq) { |
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104 | memset((char*)myOffset, 0, MAX_TRIPLES*sizeof(*myOffset)); |
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105 | SequencePosition triple(seq); |
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106 | |
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107 | mySequence = &seq; |
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108 | |
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109 | while (triple.rightOf()>=3) { // enough text for triple? |
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110 | int tidx = triple_index(triple.text()); |
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111 | TripleOffset *top = new TripleOffset(triple.leftOf(), myOffset[tidx]); |
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112 | |
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113 | myOffset[tidx] = top; |
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114 | ++triple; |
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115 | } |
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116 | } |
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117 | |
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118 | FastSearchSequence::~FastSearchSequence() { |
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119 | for (int tidx = 0; tidx<MAX_TRIPLES; ++tidx) { |
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120 | delete myOffset[tidx]; |
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121 | } |
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122 | } |
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123 | |
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124 | void AlignBuffer::correctUnalignedPositions() { |
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125 | long off = 0; |
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126 | long rest = used; |
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127 | |
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128 | while (rest) { |
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129 | const char *qual = quality(); |
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130 | char *found = (char*)memchr(qual+off, '?', rest); // search for next '?' in myQuality |
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131 | |
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132 | if (!found || (found-qual) >= rest) break; |
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133 | |
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134 | long cnt; |
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135 | for (cnt=0; found[cnt]=='?'; cnt++) found[cnt] = '+'; // count # of unaligned positions and change them to '+' |
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136 | |
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137 | long from = found-myQuality; |
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138 | long b_off = from-1; // position before unaligned positions |
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139 | long a_off = from+cnt; // positions after unaligned positions |
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140 | |
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141 | long before, after; |
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142 | for (before=0; b_off>=0 && isGlobalGap(b_off); before++, b_off--) ; // count free positions before unaligned positions |
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143 | for (after=0; a_off<used && isGlobalGap(a_off); after++, a_off++) ; // count free positions after unaligned positions |
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144 | |
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145 | if (b_off<=0) before = LONG_MAX; |
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146 | if (a_off>=used) after = LONG_MAX; |
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147 | |
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148 | if (before<after) { // move to the left |
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149 | if (before) { |
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150 | long to = from-before; |
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151 | while (cnt--) moveUnaligned(from++, to++); |
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152 | } |
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153 | } |
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154 | else if (after!=LONG_MAX) { // move to the right |
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155 | if (after) { |
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156 | from += cnt-1; // copy from right to left! |
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157 | long to = from+after; |
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158 | |
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159 | while (cnt--) moveUnaligned(from--, to--); |
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160 | } |
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161 | } |
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162 | |
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163 | rest -= (a_off-off); |
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164 | off = a_off; |
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165 | } |
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166 | } |
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167 | |
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168 | void AlignBuffer::restoreDots(CompactedSubSequence& slaveSequence) { |
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169 | long rest = used; |
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170 | long off = 0; // real position in sequence |
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171 | long count = 0; // # of bases left of this position |
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172 | long nextDot = slaveSequence.firstDotPosition(); |
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173 | |
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174 | while (nextDot!=-1 && rest) { |
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175 | unsigned char gap = myBuffer[off]; |
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176 | if (is_gap(gap)) { |
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177 | if (nextDot==count) { |
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178 | myBuffer[off] = '.'; |
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179 | } |
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180 | } |
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181 | else { |
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182 | count++; |
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183 | if (nextDot==count) { |
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184 | messagef("Couldn't restore dots at offset %li of '%s' (gap removed by aligner)", |
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185 | off, slaveSequence.name()); |
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186 | } |
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187 | if (nextDot<count) { |
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188 | nextDot = slaveSequence.nextDotPosition(); |
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189 | } |
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190 | } |
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191 | |
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192 | off++; |
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193 | rest--; |
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194 | } |
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195 | } |
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196 | |
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197 | void AlignBuffer::setDotsAtEOSequence() { |
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198 | for (int i=0; is_gap(myBuffer[i]) && i<used; ++i) myBuffer[i] = '.'; |
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199 | for (int i=used-1; is_gap(myBuffer[i]) && i>=0; --i) myBuffer[i] = '.'; |
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200 | } |
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201 | |
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202 | // -------------------------------------------------------------------------------- |
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203 | |
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204 | #ifdef UNIT_TESTS |
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205 | #include <test_unit.h> |
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206 | #include <test_helpers.h> |
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207 | |
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208 | static int get_dotpos(const CompactedSubSequence& css, int i) { |
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209 | int pos = css.firstDotPosition(); |
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210 | while (i) { |
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211 | --i; |
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212 | pos = css.nextDotPosition(); |
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213 | } |
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214 | return pos; |
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215 | } |
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216 | static int count_dotpos(const CompactedSubSequence& css) { |
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217 | int pos = css.firstDotPosition(); |
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218 | int count = 0; |
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219 | while (pos != -1) { |
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220 | ++count; |
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221 | pos = css.nextDotPosition(); |
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222 | } |
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223 | return count; |
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224 | } |
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225 | |
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226 | struct bind_css { |
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227 | CompactedSubSequence& css; |
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228 | int test_mode; |
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229 | bind_css(CompactedSubSequence& css_) : css(css_), test_mode(-1) {} |
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230 | |
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231 | int operator()(int i) const { |
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232 | switch (test_mode) { |
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233 | case 0: return css.compPosition(i); |
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234 | case 1: return css.expdPosition(i); |
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235 | case 2: return css[i]; |
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236 | case 3: return css.no_of_gaps_before(i); |
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237 | case 4: return css.no_of_gaps_after(i); |
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238 | case 5: return get_dotpos(css, i); |
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239 | } |
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240 | fa_assert(0); |
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241 | return -666; |
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242 | } |
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243 | }; |
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244 | |
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245 | #define TEST_EXPECT_CSS_SELF_REFLEXIVE(css) do { \ |
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246 | for (int b = 0; b<css.length(); ++b) { \ |
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247 | int x = css.expdPosition(b); \ |
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248 | int c = css.compPosition(x); \ |
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249 | TEST_EXPECT_EQUAL(c,b); \ |
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250 | } \ |
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251 | } while(0) |
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252 | |
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253 | #define CSS_COMMON(in,offset) \ |
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254 | int len = strlen(in); \ |
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255 | fprintf(stderr, "in='%s'\n", in); \ |
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256 | CompactedSubSequence css(in, len, "noname", offset); \ |
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257 | TEST_EXPECT_CSS_SELF_REFLEXIVE(css); \ |
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258 | bind_css bound_css(css) |
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259 | |
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260 | #define GEN_COMP_EXPD() \ |
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261 | bound_css.test_mode = 0; \ |
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262 | char *comp = collectIntFunResults(bound_css, 0, css.expdLength()-1, 3, 0, 1); \ |
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263 | bound_css.test_mode = 1; \ |
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264 | char *expd = collectIntFunResults(bound_css, 0, css.length(), 3, 0, 0) |
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265 | |
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266 | #define GEN_TEXT(in) \ |
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267 | bound_css.test_mode = 2; \ |
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268 | char *text = collectIntFunResults(bound_css, 0, css.length()-1, 3, 0, 0) |
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269 | |
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270 | #define GEN_GAPS() \ |
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271 | bound_css.test_mode = 3; \ |
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272 | char *gaps_before = collectIntFunResults(bound_css, 0, css.length(), 3, 0, 0); \ |
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273 | bound_css.test_mode = 4; \ |
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274 | char *gaps_after = collectIntFunResults(bound_css, 0, css.length()-1, 3, 0, 1) |
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275 | |
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276 | #define GEN_DOTS(in) \ |
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277 | bound_css.test_mode = 5; \ |
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278 | char *dots = collectIntFunResults(bound_css, 0, count_dotpos(css)-1, 3, 0, 1) |
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279 | |
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280 | #define FREE_COMP_EXPD() \ |
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281 | free(expd); \ |
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282 | free(comp) |
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283 | |
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284 | #define FREE_GAPS() \ |
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285 | free(gaps_before); \ |
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286 | free(gaps_after) |
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287 | |
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288 | #define COMP_EXPD_CHECK(exp_comp,exp_expd) \ |
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289 | GEN_COMP_EXPD(); \ |
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290 | TEST_EXPECT_EQUAL(comp, exp_comp); \ |
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291 | TEST_EXPECT_EQUAL(expd, exp_expd); \ |
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292 | FREE_COMP_EXPD() |
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293 | |
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294 | #define GAPS_CHECK(exp_before,exp_after) \ |
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295 | GEN_GAPS(); \ |
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296 | TEST_EXPECT_EQUAL(gaps_before, exp_before); \ |
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297 | TEST_EXPECT_EQUAL(gaps_after, exp_after); \ |
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298 | FREE_GAPS() |
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299 | |
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300 | #define DOTS_CHECK(exp_dots) \ |
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301 | GEN_DOTS(); \ |
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302 | TEST_EXPECT_EQUAL(dots, exp_dots); \ |
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303 | free(dots) |
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304 | |
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305 | // ------------------------------------------------------------ |
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306 | |
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307 | #define TEST_CS_EQUALS(in,exp_comp,exp_expd) do { \ |
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308 | CSS_COMMON(in, 0); \ |
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309 | COMP_EXPD_CHECK(exp_comp,exp_expd); \ |
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310 | } while(0) |
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311 | |
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312 | #define TEST_CS_EQUALS_OFFSET(in,offset,exp_comp,exp_expd) do { \ |
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313 | CSS_COMMON(in, offset); \ |
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314 | COMP_EXPD_CHECK(exp_comp,exp_expd); \ |
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315 | } while(0) |
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316 | |
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317 | #define TEST_GAPS_EQUALS_OFFSET(in,offset,exp_before,exp_after) do { \ |
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318 | CSS_COMMON(in, offset); \ |
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319 | GAPS_CHECK(exp_before,exp_after); \ |
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320 | } while(0) |
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321 | |
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322 | #define TEST_DOTS_EQUALS_OFFSET(in,offset,exp_dots) do { \ |
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323 | CSS_COMMON(in, offset); \ |
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324 | DOTS_CHECK(exp_dots); \ |
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325 | } while(0) |
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326 | |
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327 | #define TEST_CS_TEXT(in,exp_text) do { \ |
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328 | CSS_COMMON(in, 0); \ |
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329 | GEN_TEXT(in); \ |
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330 | TEST_EXPECT_EQUAL(text, exp_text); \ |
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331 | free(text); \ |
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332 | } while(0) |
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333 | |
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334 | #define TEST_CS_CBROKN(in,exp_comp,exp_expd) do { \ |
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335 | CSS_COMMON(in, 0); \ |
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336 | GEN_COMP_EXPD(); \ |
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337 | TEST_EXPECT_EQUAL__BROKEN(comp, exp_comp); \ |
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338 | TEST_EXPECT_EQUAL(expd, exp_expd); \ |
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339 | FREE_COMP_EXPD(); \ |
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340 | } while(0) |
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341 | |
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342 | __ATTR__REDUCED_OPTIMIZE void TEST_EARLY_CompactedSequence() { |
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343 | // reproduce a bug in compPosition |
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344 | |
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345 | // no base |
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346 | TEST_CS_EQUALS("-", " 0 [0]", " 1"); |
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347 | TEST_CS_EQUALS("--", " 0 0 [0]", " 2"); |
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348 | TEST_CS_EQUALS("---", " 0 0 0 [0]", " 3"); |
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349 | |
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350 | TEST_CS_TEXT("---", ""); |
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351 | TEST_CS_TEXT("-?~", ""); |
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352 | TEST_CS_TEXT("-A-", " 65"); // "A" |
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353 | TEST_CS_TEXT("A-C", " 65 67"); // "AC" |
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354 | |
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355 | TEST_CS_EQUALS("----------", " 0 0 0 0 0 0 0 0 0 0 [0]", " 10"); |
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356 | |
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357 | // one base |
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358 | TEST_CS_EQUALS("A---------", " 0 1 1 1 1 1 1 1 1 1 [1]", " 0 10"); |
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359 | TEST_CS_EQUALS("-A--------", " 0 0 1 1 1 1 1 1 1 1 [1]", " 1 10"); |
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360 | TEST_CS_EQUALS("---A------", " 0 0 0 0 1 1 1 1 1 1 [1]", " 3 10"); |
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361 | TEST_CS_EQUALS("-----A----", " 0 0 0 0 0 0 1 1 1 1 [1]", " 5 10"); |
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362 | TEST_CS_EQUALS("-------A--", " 0 0 0 0 0 0 0 0 1 1 [1]", " 7 10"); |
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363 | TEST_CS_EQUALS("---------A", " 0 0 0 0 0 0 0 0 0 0 [1]", " 9 10"); |
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364 | |
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365 | // two bases |
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366 | TEST_CS_EQUALS("AC--------", " 0 1 2 2 2 2 2 2 2 2 [2]", " 0 1 10"); |
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367 | |
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368 | TEST_CS_EQUALS("A-C-------", " 0 1 1 2 2 2 2 2 2 2 [2]", " 0 2 10"); |
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369 | TEST_CS_EQUALS("A--------C", " 0 1 1 1 1 1 1 1 1 1 [2]", " 0 9 10"); |
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370 | TEST_CS_EQUALS("-AC-------", " 0 0 1 2 2 2 2 2 2 2 [2]", " 1 2 10"); |
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371 | TEST_CS_EQUALS("-A------C-", " 0 0 1 1 1 1 1 1 1 2 [2]", " 1 8 10"); |
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372 | TEST_CS_EQUALS("-A-------C", " 0 0 1 1 1 1 1 1 1 1 [2]", " 1 9 10"); |
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373 | TEST_CS_EQUALS("-------A-C", " 0 0 0 0 0 0 0 0 1 1 [2]", " 7 9 10"); |
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374 | TEST_CS_EQUALS("--------AC", " 0 0 0 0 0 0 0 0 0 1 [2]", " 8 9 10"); |
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375 | |
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376 | // 3 bases |
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377 | TEST_CS_EQUALS("ACG-------", " 0 1 2 3 3 3 3 3 3 3 [3]", " 0 1 2 10"); |
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378 | TEST_CS_EQUALS("AC---G----", " 0 1 2 2 2 2 3 3 3 3 [3]", " 0 1 5 10"); |
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379 | TEST_CS_EQUALS("A-C--G----", " 0 1 1 2 2 2 3 3 3 3 [3]", " 0 2 5 10"); |
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380 | TEST_CS_EQUALS("A-C--G----", " 0 1 1 2 2 2 3 3 3 3 [3]", " 0 2 5 10"); |
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381 | TEST_CS_EQUALS("A--C-G----", " 0 1 1 1 2 2 3 3 3 3 [3]", " 0 3 5 10"); |
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382 | TEST_CS_EQUALS("A---CG----", " 0 1 1 1 1 2 3 3 3 3 [3]", " 0 4 5 10"); |
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383 | |
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384 | TEST_CS_EQUALS("A---C---G-", " 0 1 1 1 1 2 2 2 2 3 [3]", " 0 4 8 10"); |
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385 | TEST_CS_EQUALS("A---C----G", " 0 1 1 1 1 2 2 2 2 2 [3]", " 0 4 9 10"); |
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386 | TEST_CS_EQUALS("A~~~C????G", " 0 1 1 1 1 2 2 2 2 2 [3]", " 0 4 9 10"); |
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387 | |
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388 | // 4 bases |
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389 | TEST_CS_EQUALS("-AC-G--T--", " 0 0 1 2 2 3 3 3 4 4 [4]", " 1 2 4 7 10"); |
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390 | |
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391 | // 9 bases |
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392 | TEST_CS_EQUALS("-CGTACGTAC", " 0 0 1 2 3 4 5 6 7 8 [9]", " 1 2 3 4 5 6 7 8 9 10"); |
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393 | TEST_CS_EQUALS("A-GTACGTAC", " 0 1 1 2 3 4 5 6 7 8 [9]", " 0 2 3 4 5 6 7 8 9 10"); |
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394 | TEST_CS_EQUALS("ACGTA-GTAC", " 0 1 2 3 4 5 5 6 7 8 [9]", " 0 1 2 3 4 6 7 8 9 10"); |
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395 | TEST_CS_EQUALS("ACGTACGT-C", " 0 1 2 3 4 5 6 7 8 8 [9]", " 0 1 2 3 4 5 6 7 9 10"); |
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396 | TEST_CS_EQUALS("ACGTACGTA-", " 0 1 2 3 4 5 6 7 8 9 [9]", " 0 1 2 3 4 5 6 7 8 10"); |
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397 | |
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398 | // all 10 bases |
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399 | TEST_CS_EQUALS("ACGTACGTAC", " 0 1 2 3 4 5 6 7 8 9 [10]", " 0 1 2 3 4 5 6 7 8 9 10"); |
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400 | |
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401 | TEST_CS_EQUALS_OFFSET("A--C-G-", 0, " 0 1 1 1 2 2 3 [3]", " 0 3 5 7"); |
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402 | TEST_CS_EQUALS_OFFSET("A--C-G-", 2, " 0 0 0 1 1 1 2 2 3 [3]", " 2 5 7 9"); |
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403 | TEST_CS_EQUALS_OFFSET("A--C-G-", 3, " 0 0 0 0 1 1 1 2 2 3 [3]", " 3 6 8 10"); |
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404 | TEST_CS_EQUALS_OFFSET("A--C-G-", 4, " 0 0 0 0 0 1 1 1 2 2 3 [3]", " 4 7 9 11"); |
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405 | |
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406 | // test no_of_gaps_before() and no_of_gaps_after() |
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407 | TEST_GAPS_EQUALS_OFFSET("-AC---G", 0, " 1 0 3 0", " 0 3 0 [-1]"); |
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408 | TEST_GAPS_EQUALS_OFFSET(".AC..-G", 0, " 1 0 3 0", " 0 3 0 [-1]"); |
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409 | TEST_GAPS_EQUALS_OFFSET("~AC?\?-G", 0, " 1 0 3 0", " 0 3 0 [-1]"); |
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410 | TEST_GAPS_EQUALS_OFFSET("A--C-G-", 0, " 0 2 1 1", " 2 1 1 [-1]"); |
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411 | TEST_GAPS_EQUALS_OFFSET("A--C-G-", 1000, " 0 2 1 1", " 2 1 1 [-1]"); // is independent from offset |
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412 | |
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413 | // test dots |
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414 | TEST_DOTS_EQUALS_OFFSET("----ACG--T--A-C--GT----", 0, " [-1]"); // no dots |
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415 | TEST_DOTS_EQUALS_OFFSET("....ACG--T--A-C--GT....", 0, " [-1]"); // no extraordinary dots |
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416 | TEST_DOTS_EQUALS_OFFSET("....ACG--T..A-C--GT....", 0, " 4 [-1]"); // i.e. "before base number 4" |
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417 | TEST_DOTS_EQUALS_OFFSET("....ACG..T--A.C--GT....", 0, " 3 5 [-1]"); |
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418 | TEST_DOTS_EQUALS_OFFSET("....ACG..T~~A.C??GT....", 0, " 3 5 [-1]"); |
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419 | |
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420 | TEST_DOTS_EQUALS_OFFSET("AC-----GTA-----CGT", 0, " [-1]"); |
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421 | // just care THAT dots occur in a gap, dont care how many |
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422 | TEST_DOTS_EQUALS_OFFSET("A-.-G-...-C...T", 0, " 1 2 3 [-1]"); |
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423 | TEST_DOTS_EQUALS_OFFSET("A.--G...--C...T", 0, " 1 2 3 [-1]"); |
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424 | TEST_DOTS_EQUALS_OFFSET("A--.G--...C...T", 0, " 1 2 3 [-1]"); |
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425 | } |
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426 | |
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427 | #endif // UNIT_TESTS |
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428 | |
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