| 1 | # ==================================================================== # |
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| 2 | # # |
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| 3 | # File : GI.pm # |
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| 4 | # Purpose : Genome import library # |
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| 5 | # # |
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| 6 | # # |
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| 7 | # Coded by Ralf Westram (coder@reallysoft.de) in December 2003 # |
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| 8 | # Copyright Department of Microbiology (Technical University Munich) # |
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| 9 | # # |
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| 10 | # Visit our web site at: http://www.arb-home.de/ # |
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| 11 | # # |
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| 12 | # # |
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| 13 | # ==================================================================== # |
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| 14 | |
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| 15 | package GI; |
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| 16 | |
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| 17 | use strict; |
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| 18 | use warnings; |
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| 19 | use ARB; |
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| 20 | |
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| 21 | my $gb_main; |
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| 22 | my $columns; |
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| 23 | |
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| 24 | sub connectDB() { |
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| 25 | print "Connecting to running ARB database\n"; |
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| 26 | $gb_main = ARB::open(":","r"); |
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| 27 | if (! $gb_main ) { |
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| 28 | my $error = ARB::await_error(); |
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| 29 | print $error."\n"; |
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| 30 | exit 0; |
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| 31 | } |
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| 32 | ARB::begin_transaction($gb_main); |
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| 33 | } |
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| 34 | |
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| 35 | sub disconnectDB() { |
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| 36 | ARB::commit_transaction($gb_main); |
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| 37 | ARB::close($gb_main); |
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| 38 | } |
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| 39 | |
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| 40 | sub findCurrentGenome() { |
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| 41 | my $organism = BIO::read_string($gb_main, 'tmp/focus/organism_name'); # aka AWAR_ORGANISM_NAME |
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| 42 | if ($organism eq '') { |
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| 43 | error("You have to select the target organism in ARB!"); |
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| 44 | } |
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| 45 | |
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| 46 | my $gb_orga = BIO::find_organism($gb_main,$organism); |
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| 47 | if (!$gb_orga) { error("'$organism' is not a organism"); } |
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| 48 | |
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| 49 | return ($gb_orga,$organism); |
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| 50 | } |
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| 51 | |
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| 52 | sub unmarkGenesOfGenome($) { |
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| 53 | my ($gb_genome) = @_; |
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| 54 | my $gb_gene = BIO::first_marked_gene($gb_genome); |
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| 55 | while ($gb_gene) { |
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| 56 | ARB::write_flag($gb_gene, 0); # unmark |
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| 57 | $gb_gene = BIO::next_marked_gene($gb_gene); |
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| 58 | } |
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| 59 | } |
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| 60 | |
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| 61 | sub findORF($$$$$) { |
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| 62 | my ($gb_gene_data,$genome_name,$orf,$create,$verbose) = @_; |
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| 63 | my $error; |
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| 64 | my $gb_orf; |
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| 65 | my $gb_locus_tag = ARB::find_string($gb_gene_data, "locus_tag", $orf, 1, "down_2"); |
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| 66 | if (!$gb_locus_tag) { |
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| 67 | if ($create==0) { |
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| 68 | $error = "no gene with locus_tag '$orf' found for organism '$genome_name'"; |
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| 69 | } |
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| 70 | else { |
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| 71 | my $gb_genome = ARB::get_father($gb_gene_data); |
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| 72 | $gb_orf = BIO::create_nonexisting_gene($gb_genome, $orf); |
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| 73 | if (!$gb_orf) { |
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| 74 | my $reason = ARB::get_error(); |
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| 75 | $error = "cannot create gene '$orf' ($reason)"; |
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| 76 | } |
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| 77 | else { |
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| 78 | my $gb_locus_tag = ARB::search($gb_orf, "locus_tag", "STRING"); |
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| 79 | if (!$gb_locus_tag) { |
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| 80 | my $reason = ARB::get_error(); |
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| 81 | $error = "cannot create field 'locus_tag' ($reason)"; |
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| 82 | } |
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| 83 | else { |
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| 84 | $error = ARB::write_string($gb_locus_tag, $orf); |
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| 85 | if ($error) { |
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| 86 | $error = "Couldn't write to 'locus_tag' ($error)"; |
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| 87 | } |
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| 88 | } |
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| 89 | } |
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| 90 | if (!$error and $verbose==1) { print "Created new gene '$orf'\n"; } |
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| 91 | } |
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| 92 | } |
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| 93 | else { |
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| 94 | $gb_orf = ARB::get_father($gb_locus_tag); |
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| 95 | } |
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| 96 | |
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| 97 | if (!$gb_orf) { if (!$error) { die "Internal error"; }} |
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| 98 | return ($gb_orf,$error); |
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| 99 | } |
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| 100 | |
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| 101 | sub write_entry($$$$$$) { |
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| 102 | my ($gb_container, $field_name, $field_type, $field_content, $overwrite, $verbose) = @_; |
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| 103 | my $gb_field = ARB::search($gb_container, $field_name, "NONE"); |
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| 104 | my $error; |
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| 105 | if (!$gb_field) { |
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| 106 | $gb_field = ARB::search($gb_container, $field_name, $field_type); |
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| 107 | if (!$gb_field) { |
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| 108 | my $reason = ARB::get_error(); |
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| 109 | $error = "Can't create '$field_name' ($reason)"; |
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| 110 | } |
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| 111 | } |
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| 112 | else { |
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| 113 | if ($overwrite==0) { |
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| 114 | $error = "Field '$field_name' already exists"; |
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| 115 | } |
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| 116 | } |
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| 117 | |
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| 118 | if (!$error) { |
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| 119 | if (!$gb_field) { die "internal error"; } |
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| 120 | $error = ARB::write_as_string($gb_field, $field_content); |
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| 121 | if ($error) { $error = "Cannot write to '$field_name' ($error)"; } |
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| 122 | } |
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| 123 | |
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| 124 | return $error; |
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| 125 | } |
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| 126 | |
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| 127 | # -------------------------------------------------------------------------------- |
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| 128 | |
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| 129 | sub show_csv_info() { |
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| 130 | print " CSV may be saved with Excel and StarCalc. It simply is a\n". |
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| 131 | " comma separated list with strings quoted in \"\". The first line\n". |
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| 132 | " contains the column titles.\n"; |
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| 133 | } |
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| 134 | |
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| 135 | sub message($) { |
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| 136 | my ($msg) = @_; |
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| 137 | BIO::message($gb_main, $msg); |
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| 138 | print "$msg\n"; |
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| 139 | } |
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| 140 | |
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| 141 | sub error($) { |
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| 142 | my ($msg) = @_; |
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| 143 | $msg = "Error: ".$msg; |
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| 144 | ARB::commit_transaction($gb_main); # this undoes all changes made by this script |
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| 145 | # ARB::abort_transaction($gb_main); # this undoes all changes made by this script |
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| 146 | |
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| 147 | ARB::begin_transaction($gb_main); |
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| 148 | BIO::message($gb_main, $msg); |
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| 149 | BIO::message($gb_main, "Script aborted!"); |
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| 150 | ARB::commit_transaction($gb_main); |
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| 151 | die $msg."\n"; |
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| 152 | } |
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| 153 | |
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| 154 | # -------------------------------------------------------------------------------- |
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| 155 | |
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| 156 | sub define_tokenizer_columns($) { |
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| 157 | ($columns) = @_; |
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| 158 | } |
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| 159 | |
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| 160 | sub tokenize_columns($$) { |
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| 161 | my ($line,$errline) = @_; |
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| 162 | chomp $line; |
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| 163 | $line .= ','; |
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| 164 | |
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| 165 | my @array = (); |
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| 166 | |
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| 167 | while (not $line =~ /^[ ]*$/ig) { |
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| 168 | if ($line =~ /^[ ]*\"([^\"]*)\"[ ]*,/ig) { |
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| 169 | my $content = $1; |
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| 170 | $line = $'; |
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| 171 | $content =~ s/^[ ]*//ig; |
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| 172 | $content =~ s/[ ]*$//ig; |
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| 173 | push @array, $content; |
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| 174 | } |
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| 175 | elsif ($line =~ /[ ]*([0-9]+)[ ]*,/ig) { |
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| 176 | push @array, $1; |
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| 177 | $line = $'; |
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| 178 | } |
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| 179 | else { |
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| 180 | error("cannot parse line $errline (at '$line')"); |
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| 181 | } |
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| 182 | } |
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| 183 | |
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| 184 | my $cols = @array; |
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| 185 | if ($cols != $columns) { |
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| 186 | error("expected $columns columns (found $cols) in line $errline"); |
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| 187 | } |
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| 188 | |
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| 189 | return @array; |
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| 190 | } |
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| 191 | |
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| 192 | # -------------------------------------------------------------------------------- |
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| 193 | |
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| 194 | 1; # result of module initialization |
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| 195 | |
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