1 | #Please insert up references in the next lines (line starts with keyword UP) |
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2 | UP arb.hlp |
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3 | UP glossary.hlp |
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4 | UP pars.hlp |
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5 | |
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6 | #Please insert subtopic references (line starts with keyword SUB) |
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7 | SUB pa_add.hlp |
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8 | |
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9 | # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} |
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10 | |
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11 | #************* Title of helpfile !! and start of real helpfile ******** |
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12 | TITLE Add species without optimizing topology |
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13 | |
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14 | OCCURRENCE ARB_NT/Tree/Add Species to Existing Tree/ARB Parsimony (Quick add marked) |
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15 | ARB_PARSIMONY/Tree/Add Species to Tree/Add Marked Species |
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16 | ARB_PARSIMONY/Tree/Add Species to Tree/Add Selected Species |
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17 | ARB_PARSIMONY/Tree/Add Species to Tree/Remove & Add Marked Species |
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18 | |
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19 | DESCRIPTION All affected 'species' are positioned according to maximum |
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20 | parsimony criteria. The current tree topology will not be optimized |
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21 | after insertion. |
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22 | |
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23 | In case of 'Add Selected Species' the |
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24 | currently 'selected species' (see LINK{glossary.hlp}) gets |
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25 | inserted. The other functions always work on all 'marked species'. |
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26 | |
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27 | In case of 'Remove & Add Marked Species' any marked species are first |
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28 | removed from the tree and then inserted. The other functions only add |
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29 | those marked species which are not already present in the tree. |
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30 | |
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31 | |
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32 | SECTION Full length vs partial sequence data |
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33 | |
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34 | This function only works correct when adding full-length sequences (FLS), |
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35 | where full-length here applies to the resulting positions after |
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36 | applying the selected alignment filter(s). |
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37 | |
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38 | Any partially missing sequence data will be considered an deletion and |
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39 | adds extra distance between full- and partial sequences. |
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40 | |
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41 | One common effect of doing so, is that partial sequences often group together |
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42 | although they obviously are not (parts of) near relatives, just because they |
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43 | share the same or similar alignment regions. |
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44 | When the amount of missing data (in one seq.; compared to FLS) is greater |
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45 | than the length of the shared alignment region, the partial sequences will |
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46 | prefer to group together. |
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47 | |
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48 | To avoid that problem |
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49 | - do not use partial sequence data (recommended) or |
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50 | - (if you really have to) you should declare and handle partial sequence |
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51 | data as such, by using LINK{pa_partial.hlp}. |
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52 | |
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53 | |
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54 | SECTION Tree degeneration |
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55 | |
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56 | As no topology optimization takes place here, the tree will tend to |
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57 | degenerate while you add more and more species, i.e. the tree will |
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58 | continuously diverge from an optimized topology build upon the same |
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59 | set of species. |
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60 | |
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61 | Nevertheless this function has proven useful to determine the |
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62 | phylogenetic position of new/custom sequences in an optimized tree. |
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63 | But you should always be aware that the more sequences you add this way, |
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64 | the worse your topology will get. |
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65 | |
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66 | Probably you may want to optimize your tree from time |
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67 | to time, e.g. using LINK{pa_optimizer.hlp}. |
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68 | |
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69 | |
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70 | SECTION Insertion order |
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71 | |
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72 | Due to the tree degeneration mentioned above, the insertion |
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73 | order of the species you add to an existing tree is of some |
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74 | importance. |
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75 | |
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76 | This function sorts the species (added in each batch of species) by the number |
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77 | of base positions (bp) remaining after filtering. Species with more bp get |
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78 | inserted before species with less bp - as their position in the tree will be |
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79 | determined stronger (there is no strict order for species with same amount of bp). |
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80 | |
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81 | |
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82 | NOTES The current parsimony value (number of base changes needed) is |
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83 | displayed after the 'Current Par' prompt in the upper part of |
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84 | the 'ARB_PARSIMONY' window and the 'Message' window (pops up |
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85 | while calculating). |
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86 | |
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87 | EXAMPLES None |
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88 | |
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89 | WARNINGS The phylogenetic information conferred by the new sequence(s) is |
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90 | not used for global tree optimization. |
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91 | |
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92 | BUGS No bugs known |
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