1 | #FIG 3.2 |
---|
2 | Landscape |
---|
3 | Center |
---|
4 | Metric |
---|
5 | A4 |
---|
6 | 100.00 |
---|
7 | Single |
---|
8 | -2 |
---|
9 | 1200 2 |
---|
10 | 6 1755 1215 8280 1620 |
---|
11 | 2 1 0 2 -1 -1 0 0 -1 0.000 0 0 7 0 0 2 |
---|
12 | 1785 1575 8235 1575 |
---|
13 | 4 1 -1 0 0 18 12 0.0000 4 180 6465 5010 1380 This module searches for specific PCR-primers in the current sequence. \001 |
---|
14 | -6 |
---|
15 | 6 720 8280 3105 8460 |
---|
16 | 4 0 0 50 0 16 12 0.0000 4 150 555 2520 8415 $result\001 |
---|
17 | 4 0 0 50 0 16 12 0.0000 4 180 660 720 8415 $design\001 |
---|
18 | -6 |
---|
19 | 6 2340 4500 5805 5220 |
---|
20 | 4 0 -1 0 0 18 12 0.0000 4 135 840 2340 4635 G+C ratio\001 |
---|
21 | 4 0 -1 0 0 18 12 0.0000 4 180 1095 2340 5085 temperature\001 |
---|
22 | 4 0 0 50 0 16 12 0.0000 4 180 600 3960 4725 $mingc\001 |
---|
23 | 4 0 0 50 0 16 12 0.0000 4 180 780 3960 5175 $mintemp\001 |
---|
24 | 4 0 0 50 0 16 12 0.0000 4 180 645 4950 4725 $maxgc\001 |
---|
25 | 4 0 0 50 0 16 12 0.0000 4 180 825 4950 5175 $maxtemp\001 |
---|
26 | -6 |
---|
27 | 6 2340 3060 5670 4140 |
---|
28 | 6 4005 3060 5400 3195 |
---|
29 | 4 0 -1 0 0 18 12 0.0000 4 135 360 4005 3195 MIN\001 |
---|
30 | 4 0 -1 0 0 18 12 0.0000 4 135 405 4995 3195 MAX\001 |
---|
31 | -6 |
---|
32 | 6 2340 3420 5670 4140 |
---|
33 | 4 0 -1 0 0 18 12 0.0000 4 180 1170 2340 3555 primer length\001 |
---|
34 | 4 0 -1 0 0 18 12 0.0000 4 180 1380 2340 4005 primer distance\001 |
---|
35 | 4 0 0 50 0 16 12 0.0000 4 150 675 3960 4095 $mindist\001 |
---|
36 | 4 0 0 50 0 16 12 0.0000 4 150 720 4950 4095 $maxdist\001 |
---|
37 | 4 0 0 50 0 16 12 0.0000 4 150 690 4950 3645 $maxlen\001 |
---|
38 | 4 0 0 50 0 16 12 0.0000 4 150 645 3960 3645 $minlen\001 |
---|
39 | -6 |
---|
40 | -6 |
---|
41 | 6 2340 2160 5760 2880 |
---|
42 | 4 0 -1 0 0 18 12 0.0000 4 180 1155 2340 2745 right position\001 |
---|
43 | 4 0 -1 0 0 18 12 0.0000 4 180 1050 2340 2295 left position\001 |
---|
44 | 4 0 0 50 0 16 12 0.0000 4 180 750 3960 2835 $minright\001 |
---|
45 | 4 0 -1 0 0 16 12 0.0000 4 150 630 3960 2385 $minleft\001 |
---|
46 | 4 0 -1 0 0 16 12 0.0000 4 150 675 4950 2385 $maxleft\001 |
---|
47 | 4 0 0 50 0 16 12 0.0000 4 180 795 4950 2835 $maxright\001 |
---|
48 | -6 |
---|
49 | 6 4005 1800 6840 1935 |
---|
50 | 4 0 -1 0 0 18 12 0.0000 4 135 630 4005 1935 START\001 |
---|
51 | 4 0 -1 0 0 18 12 0.0000 4 135 1845 4995 1935 NUMBER OF BASES\001 |
---|
52 | -6 |
---|
53 | 6 7020 3060 7470 3600 |
---|
54 | 4 0 0 50 0 16 12 0.0000 4 150 450 7020 3555 $init2\001 |
---|
55 | 4 0 0 50 0 16 12 0.0000 4 150 450 7020 3195 $init1\001 |
---|
56 | -6 |
---|
57 | 2 2 0 1 -1 -1 0 0 -1 0.000 0 0 0 0 0 5 |
---|
58 | 9135 8685 9135 600 585 600 585 8685 9135 8685 |
---|
59 | 2 1 0 2 -1 -1 0 0 -1 0.000 0 0 7 0 0 2 |
---|
60 | 720 7875 9000 7875 |
---|
61 | 2 1 0 1 0 7 50 0 -1 0.000 0 0 7 0 0 2 |
---|
62 | 720 4260 9000 4260 |
---|
63 | 2 1 0 1 0 7 50 0 -1 0.000 0 0 7 0 0 2 |
---|
64 | 720 5340 9000 5340 |
---|
65 | 2 2 0 1 -1 -1 0 0 -1 0.000 0 0 0 0 0 5 |
---|
66 | 8865 3735 8865 2025 6345 2025 6345 3735 8865 3735 |
---|
67 | 4 0 0 50 0 16 12 0.0000 4 150 555 720 1035 $close\001 |
---|
68 | 4 2 0 50 0 16 12 0.0000 4 180 465 9000 1035 $help\001 |
---|
69 | 4 2 0 50 0 16 10 0.0000 4 150 2355 9045 8595 written by Wolfram Foerster 2001\001 |
---|
70 | 4 0 0 50 0 18 12 0.0000 4 180 870 6345 4005 0 = ignore\001 |
---|
71 | 4 2 -1 0 0 18 12 0.0000 4 180 2940 3780 5715 min. dist to next match of primer\001 |
---|
72 | 4 2 -1 0 0 18 12 0.0000 4 180 1830 3780 6165 expand IUPAC codes\001 |
---|
73 | 4 2 -1 0 0 18 12 0.0000 4 135 2070 3780 6615 max. number of results\001 |
---|
74 | 4 2 -1 0 0 18 12 0.0000 4 135 1110 3780 7065 G+C - factor\001 |
---|
75 | 4 2 -1 0 0 18 12 0.0000 4 180 1695 3780 7515 temperature factor\001 |
---|
76 | 4 0 -1 0 0 18 12 0.0000 4 180 1860 5130 5715 <= 0 : singular match\001 |
---|
77 | 4 0 0 50 0 16 12 0.0000 4 180 1065 3960 7605 $temp_factor\001 |
---|
78 | 4 0 0 50 0 16 12 0.0000 4 165 945 3960 7155 $GC_factor\001 |
---|
79 | 4 0 0 50 0 16 12 0.0000 4 180 945 3960 6705 $max_pairs\001 |
---|
80 | 4 0 0 50 0 16 12 0.0000 4 180 1380 3960 6255 $expand_IUPAC\001 |
---|
81 | 4 0 0 50 0 16 12 0.0000 4 165 1350 3960 5805 $allowed_match\001 |
---|
82 | 4 0 0 50 0 16 35 0.0000 4 210 75 5850 7380 }\001 |
---|
83 | 4 0 -1 0 0 18 12 0.0000 4 135 660 5130 7515 0 .. 100\001 |
---|
84 | 4 0 -1 0 0 18 12 0.0000 4 135 660 5130 7065 0 .. 100\001 |
---|
85 | 4 0 0 50 0 18 12 0.0000 4 180 1830 6030 7290 G+C + temp = 100\001 |
---|
86 | 4 0 0 50 0 16 12 0.0000 4 180 1050 7425 8280 $aprox_mem\001 |
---|
87 | 4 0 0 50 0 18 12 0.0000 4 180 2490 4815 8190 estimated memory needed :\001 |
---|
88 | 4 1 0 50 0 18 12 0.0000 4 135 2055 7605 2565 with reasonable values\001 |
---|
89 | 4 1 0 50 0 18 12 0.0000 4 180 2250 7605 2340 fill positions and distance\001 |
---|
90 | 4 1 0 50 0 18 12 0.0000 4 135 1650 7605 2790 from the current ..\001 |
---|
91 | 4 0 0 50 0 0 12 0.0000 4 195 555 3780 8325 $config\001 |
---|