1 | #Please insert up references in the next lines (line starts with keyword UP) |
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2 | UP arb.hlp |
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3 | UP glossary.hlp |
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4 | UP rna3d_general.hlp |
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5 | |
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6 | #Please insert subtopic references (line starts with keyword SUB) |
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7 | #SUB subtopic.hlp |
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8 | |
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9 | # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} |
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10 | |
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11 | #************* Title of helpfile !! and start of real helpfile ******** |
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12 | TITLE Mapping Secondary Structural Information |
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13 | |
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14 | OCCURRENCE In ARB primary structure editor (ARB_EDIT4) -> RNA3D |
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15 | |
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16 | DESCRIPTION |
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17 | |
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18 | Secondary and tertiary structure interactions of the well established comparative |
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19 | structure models of rRNA which are used in SECEDIT to generate 2D structure models are |
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20 | fitted to the three-dimensional structure of E. coli master sequence. |
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21 | |
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22 | DISPLAY HELICES: |
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23 | |
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24 | Enabling this check box maps the secondary structural motifs (loops, helices |
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25 | and bulges) onto the molecule. Secondary structural information is according |
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26 | to the comparative models of rRNA used in primary and secondary structure |
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27 | editors. |
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28 | |
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29 | HELIX SKELETON: |
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30 | |
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31 | Enabling will draw a skeleton of secondary structure mask. By setting a grey |
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32 | or light color you can achieve transparent mask avoiding any interference with |
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33 | other information overlays. |
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34 | |
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35 | DISPLAY MID-HELIX: |
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36 | |
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37 | This displays middle point of the helices. |
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38 | |
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39 | DISPLAY HELIX NUMBER: |
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40 | |
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41 | Checking this box will display the corresponding helix numbers in the rRNA 3D |
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42 | structure. Helix numbers are according to ARB numbering scheme. The small |
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43 | subunit (16S) rRNA of E.coli contains 50 helices which are numbered from 1 to |
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44 | 50. |
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45 | |
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46 | DISPLAY NUMBER OF HELICES: |
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47 | |
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48 | Using this you can set the number of helices you would like to be displayed in |
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49 | the 3D molecule. This feature is very helpful to thoroughly examine the |
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50 | specific helices in the structure. |
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51 | |
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52 | HELIX SIZE: |
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53 | |
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54 | Thickness or the size of the helices can be set by specifying the desired |
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55 | value in this box. |
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56 | |
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57 | DISPLAY TERTIARY INTERACTIONS: |
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58 | |
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59 | The tertiary interactions observed in small subunit rRNA can be displayed in |
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60 | the three-dimensional conformations of small subunit rRNA by enabling this |
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61 | check box. The tertiary information data is from Gautheret et al. |
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62 | |
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63 | Color settings related to helix, skeleton, mid-helix, helix number and tertiary |
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64 | interactions can be changed using âColor Settingsâ of the main RNA3D window. |
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65 | |
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66 | NOTES |
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67 | |
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68 | Gautheret D, Damberger SH, Gutell RR: Identification of base-triples in RNA using |
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69 | comparative sequence analysis. J Mol Biol 1995, 248: 27-43. |
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70 | |
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71 | |
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72 | EXAMPLES None |
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73 | |
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74 | WARNINGS None |
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75 | |
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76 | BUGS No bugs known |
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