1 | #Please insert up references in the next lines (line starts with keyword UP) |
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2 | UP arb.hlp |
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3 | UP glossary.hlp |
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4 | UP mark.hlp |
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5 | |
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6 | #Please insert subtopic references (line starts with keyword SUB) |
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7 | #SUB nt_align_select.hlp |
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8 | |
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9 | # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} |
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10 | |
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11 | #************* Title of helpfile !! and start of real helpfile ******** |
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12 | TITLE How to get an initial tree |
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13 | |
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14 | DESCRIPTION The main idea of ARB is to manage database access via a tree. |
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15 | |
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16 | [ You don't have a tree, but you may access all |
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17 | species data using 'Species/Search and Query'. ] |
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18 | |
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19 | There are several ways to construct an initial tree: |
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20 | |
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21 | Align the sequence data: |
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22 | |
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23 | 1. Mark all 'species' (see LINK{glossary.hlp}) |
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24 | Choose the item 'Mark all Species' |
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25 | from the menu 'Species' |
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26 | of the 'ARB_NT' main window (this program) |
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27 | (normally this is referred as: 'ARB_NT/Species/Mark all Species'); |
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28 | |
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29 | 2. Select an alignment: |
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30 | <ARB_NT/3rd big Button in top area> |
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31 | (see LINK{nt_align_select.hlp}) |
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32 | |
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33 | 3. To align the marked sequences use one of the provided aligners |
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34 | in 'ARB_NT/Sequence/Align Sequences' |
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35 | |
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36 | Reconstruct an initial tree: |
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37 | |
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38 | To get a good tree, you should use different treeing |
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39 | methods. |
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40 | |
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41 | To quickly get an initial tree we recommend to use |
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42 | neighbour joining for DNA, RNA and Protein sequences: |
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43 | |
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44 | To start neighbour joining select 'ARB_NT/Tree/Build tree from sequence data/Distance matrix methods/ARB Neighbor Joining', |
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45 | to display the 'NEIGHBOUR JOINING [ARB_DIST]' window. |
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46 | Refer to LINK{dist.hlp} for setting parameters of tree reconstruction. |
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47 | |
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48 | Show the reconstructed tree: |
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49 | |
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50 | Click on the <tree_*> (2nd big rectangular) button in |
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51 | top area of ARB_NT and choose the respective tree. |
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52 | |
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53 | Save everything: |
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54 | |
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55 | Choose the 'Save Whole Database as' item from the 'File' menu. |
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56 | |
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57 | NOTES None |
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58 | |
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59 | WARNINGS None |
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60 | |
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61 | BUGS No bugs known |
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