1 | item:ProbCons |
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2 | itemmeta: P |
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3 | seqtype:* |
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4 | itemmethod:(GOTO_LOCAL_DIR; \ |
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5 | arb_sed 's/#/>HASH_/' <TmpInputFile | \ |
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6 | arb_sed 's/%/>PERCENT_/' | \ |
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7 | arb_sed 's/"/>QUOTE_/'| \ |
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8 | arb_sed 's/@/>AT_/' | \ |
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9 | arb_sed '/^[^>]/ y/./-/' > probcons_in; \ |
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10 | RUN_IN_WINDOW(($ARBHOME/bin/probcons -pre $PRE_TRAINING_REPS -ir $PASSES -c $REPS probcons_in > probcons_out;)); \ |
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11 | arb_sed 's/>HASH_/#/' <probcons_out | \ |
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12 | arb_sed 's/>PERCENT_/%/' | \ |
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13 | arb_sed 's/>QUOTE_/"/' | \ |
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14 | arb_sed 's/>AT_/@/' > TmpOutputFile; RM_LOCAL_FILES(probcons_in* probcons_out* TmpInputFile) ) |
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15 | |
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16 | arg:REPS |
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17 | argtype:choice_menu |
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18 | arglabel:Passes of consistency transformation |
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19 | argtype:slider |
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20 | argmin:0 |
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21 | argmax:5 |
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22 | argvalue:2 |
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23 | |
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24 | |
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25 | arg:PASSES |
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26 | arglabel:Passes of iterative refinement |
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27 | argtype:slider |
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28 | argmin:0 |
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29 | argmax:1000 |
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30 | argvalue:100 |
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31 | |
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32 | arg:PRE_TRAINING_REPS |
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33 | arglabel:Rounds of pre-training before aligning the sequences |
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34 | argtype:slider |
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35 | argmin:0 |
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36 | argmax:20 |
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37 | argvalue:0 |
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38 | |
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39 | in:TmpInputFile |
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40 | informat:flat |
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41 | intyped:detailed |
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42 | insave: |
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43 | |
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44 | out:TmpOutputFile |
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45 | outformat:flat |
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46 | |
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47 | //---------------------------------------------------- |
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48 | |
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49 | item:Muscle |
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50 | itemmeta: M |
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51 | seqtype:* |
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52 | itemmethod:(GOTO_LOCAL_DIR; \ |
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53 | arb_sed 's/#/>HASH_/' <TmpInputFile | \ |
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54 | arb_sed 's/%/>PERCENT_/' | \ |
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55 | arb_sed 's/"/>QUOTE_/'| \ |
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56 | arb_sed 's/@/>AT_/' | \ |
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57 | arb_sed '/^[^>]/ y/./-/' > muscle_in; \ |
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58 | RUN_IN_WINDOW(($ARBHOME/bin/muscle $SCORE $DIAGS $ANCHORS $DIMER $BRENNER -in muscle_in -out muscle_out -maxiters $MAXITERS;)); \ |
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59 | arb_sed 's/>HASH_/#/' <muscle_out | \ |
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60 | arb_sed 's/>PERCENT_/%/' | \ |
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61 | arb_sed 's/>QUOTE_/"/' | \ |
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62 | arb_sed 's/>AT_/@/' > TmpOutputFile; RM_LOCAL_FILES(muscle_in* muscle_out* TmpInputFile)) |
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63 | |
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64 | arg:MAXITERS |
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65 | argtype:text |
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66 | arglabel:Maximum number of iterations to run(Use 2 for large datasets) |
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67 | argtext:16 |
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68 | |
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69 | //arg:ANCHORS |
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70 | //argtype:choice_menu |
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71 | //arglabel:Use anchor optimization in tree dependent refinement iterations |
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72 | //argchoice:Yes(default):-anchors |
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73 | //argchoice:No:-noanchors |
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74 | |
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75 | //arg:BRENNER |
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76 | //argtype:choice_menu |
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77 | //arglabel:Use Steven Brenner's method for computing the root alignment |
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78 | //argchoice:No(default): |
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79 | //argchoice:Yes:-brenner |
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80 | |
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81 | //arg:DIMER |
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82 | //argtype:choice_menu |
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83 | //arglabel:Use dimer approximation for the SP score\n(faster, slightly less accurate) |
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84 | //argchoice:No(default): |
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85 | //argchoice:Yes:-dimer |
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86 | |
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87 | //arg:DIAGS |
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88 | //argtype:choice_menu |
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89 | //arglabel:Use diagonal optimizations\n(faster, especially for closely related sequences,\nbut may be less accurate) |
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90 | //argchoice:No(default): |
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91 | //argchoice:Yes:-diags |
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92 | |
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93 | //arg:SCORE |
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94 | //argtype:choice_menu |
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95 | //arglabel:Scoring |
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96 | //argchoice:Log-expectation profile score (VTML240)(default):-le |
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97 | //argchoice:Sum-of-pairs protein profile score (PAM200):-sp |
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98 | //argchoice:Sum-of-pairs profile score (VTML240):-sv |
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99 | |
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100 | |
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101 | in:TmpInputFile |
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102 | informat:flat |
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103 | intyped:detailed |
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104 | insave: |
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105 | |
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106 | out:TmpOutputFile |
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107 | outformat:flat |
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108 | |
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109 | //------------------------------------------------------------------- |
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110 | |
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111 | item:Mafft |
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112 | itemmeta: f |
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113 | seqtype:* |
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114 | itemmethod:(GOTO_LOCAL_DIR; \ |
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115 | arb_sed 's/#/>HASH_/' <TmpInputFile | \ |
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116 | arb_sed 's/%/>PERCENT_/' | \ |
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117 | arb_sed 's/"/>QUOTE_/'| \ |
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118 | arb_sed 's/@/>AT_/' | \ |
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119 | arb_sed '/^[^>]/ y/./-/' > mafft_in; \ |
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120 | RUN_IN_WINDOW(($ARBHOME/bin/$COMMAND $METHOD --preservecase --ep $OFFSET $SCORING_MATRIX mafft_in > mafft_out;)); \ |
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121 | arb_sed 's/>HASH_/#/' <mafft_out | \ |
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122 | arb_sed 's/>PERCENT_/%/' | \ |
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123 | arb_sed 's/>QUOTE_/"/' | \ |
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124 | arb_sed 's/>AT_/@/' > TmpOutputFile; RM_LOCAL_FILES(mafft_in* TmpInputFile mafft_out*)) |
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125 | |
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126 | itemhelp:mafft.help |
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127 | |
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128 | |
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129 | arg:COMMAND |
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130 | argtype:choice_menu |
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131 | arglabel:Method |
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132 | argchoice:Auto:mafft --auto |
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133 | argchoice:L-INS-i:mafft-linsi |
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134 | argchoice:G-INS-i:mafft-ginsi |
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135 | argchoice:E-INS-i:mafft-einsi |
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136 | argchoice:Q-INS-i:mafft-qinsi |
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137 | argchoice:X-INS-i:mafft-xinsi |
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138 | argchoice:FFT-NS-i:mafft-fftnsi |
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139 | argchoice:FFT-NS-2:mafft-fftns |
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140 | argchoice:NW-NS-i:mafft-nwnsi |
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141 | argchoice:NW-NS-2:mafft-nwns |
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142 | |
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143 | arg:SCORING_MATRIX |
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144 | argtype:choice_menu |
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145 | arglabel:Scoring matrix |
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146 | argchoice:BLOSUM62:--bl 62 |
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147 | argchoice:BLOSUM30:--bl 30 |
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148 | argchoice:BLOSUM45:--bl 45 |
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149 | argchoice:BLOSUM80:--bl 80 |
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150 | argchoice:JTT100:--jtt 100 |
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151 | argchoice:JTT200:--jtt 200 |
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152 | argchoice:JTT200:--jtt 200 |
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153 | argchoice:1PAM / K=2:--kimura 1 |
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154 | argchoice:20PAM / K=2:--kimura 20 |
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155 | argchoice:200PAM / K=2:--kimura 200 |
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156 | |
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157 | |
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158 | arg:OFFSET |
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159 | argtype:text |
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160 | arglabel:Offset value [0..1]\n(Use Values >0.1 for closely related sequences) |
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161 | argtext:0.5 |
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162 | |
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163 | |
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164 | in:TmpInputFile |
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165 | informat:flat |
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166 | intyped:detailed |
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167 | insave: |
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168 | |
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169 | out:TmpOutputFile |
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170 | outformat:flat |
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171 | |
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172 | //-------------------------------------------------------- |
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173 | |
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174 | item:ClustalW automatic |
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175 | itemmeta: W |
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176 | seqtype:* |
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177 | itemmethod:(GOTO_LOCAL_DIR; \ |
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178 | tr '"%//' '>' <TmpInputFile >clus_in; \ |
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179 | RUN_IN_WINDOW((clustalw -output=PIR -infile=clus_in -align ; \ |
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180 | cat clus_in.pir | sed "s/^>DL;/#/g" | sed "s/^>P1;/%/g" | sed "s/^\*$//g" >TmpOutputFile; )); \ |
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181 | RM_LOCAL_FILES(clus_in* TmpInputFile)) |
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182 | |
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183 | itemhelp:clustalw.help |
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184 | |
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185 | |
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186 | in:TmpInputFile |
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187 | informat:flat |
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188 | insave: |
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189 | |
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190 | out:TmpOutputFile |
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191 | outformat:flat |
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192 | |
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193 | // -------------------------------------------------------------------------------- |
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194 | |
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195 | item:Create ClustalW Profile 2 |
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196 | itemmeta: C |
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197 | seqtype:* |
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198 | itemmethod:(GOTO_LOCAL_DIR ; tr '"%//' '>' <TmpInputFile >../clus_prf ; RM_LOCAL_FILES(TmpInputFile)) |
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199 | |
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200 | itemhelp:clustalw.help |
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201 | |
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202 | in:TmpInputFile |
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203 | informat:flat |
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204 | insave: |
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205 | |
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206 | // -------------------------------------------------------------------------------- |
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207 | |
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208 | item:ClustalW Profile Alignment |
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209 | itemmeta: A |
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210 | seqtype:* |
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211 | itemmethod:(GOTO_LOCAL_DIR; \ |
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212 | tr '"%//' '>' <TmpInputFile >clus_in; \ |
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213 | RUN_IN_WINDOW((clustalw -output=PIR -profile1=clus_in -profile2=../clus_prf -align ; \ |
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214 | cat clus_in.pir | arb_sed "s/^>DL;/#/g" | sed "s/^>P1;/%/g" | arb_sed "s/^\*$//g" >TmpOutputFile; )); \ |
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215 | RM_LOCAL_FILES(clus_in* TmpInputFile)) |
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216 | |
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217 | itemhelp:clustalw.help |
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218 | |
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219 | |
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220 | in:TmpInputFile |
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221 | informat:flat |
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222 | insave: |
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223 | |
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224 | out:TmpOutputFile |
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225 | outformat:flat |
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226 | |
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227 | // -------------------------------------------------------------------------------- |
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228 | |
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229 | item:ClustalW DNA Alignment (fast) |
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230 | seqtype: N |
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231 | itemmeta: N |
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232 | itemmethod:(GOTO_LOCAL_DIR; \ |
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233 | tr '"%//' '>' <TmpInputFile >clus_in ; \ |
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234 | RUN_IN_WINDOW((clustalw -output=PIR -infile=clus_in -align \ |
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235 | -quicktree $INTERACTIVE \ |
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236 | -ktuple=$KTUPLE -topdiags=$TOPDIAGS \ |
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237 | -window=$WINDOW -pairgap=$PAIRGAP \ |
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238 | -score=$SCORE \ |
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239 | -type=DNA -transweight=$TRANSWEIGHT \ |
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240 | -gapopen=$GOP -gapext=$GEP -maxdiv=$MAXDIV \ |
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241 | -dnamatrix=$DNAMATRIX; \ |
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242 | cat clus_in.pir | arb_sed "s/^>DL;/#/g" | arb_sed "s/^>P1;/%/g" | arb_sed "s/^\*$//g" >TmpOutputFile; )) ; \ |
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243 | RM_LOCAL_FILES(clus_in* TmpInputFile)) |
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244 | |
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245 | itemhelp:clustalw.help |
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246 | |
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247 | // parameters for fast pairwise alignment: |
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248 | |
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249 | arg:PAIRGAP |
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250 | arglabel:Gap penalty (pairwise ali) |
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251 | argtype:slider |
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252 | argmin:1 |
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253 | argmax:500 |
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254 | argvalue:5 |
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255 | |
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256 | arg:KTUPLE |
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257 | arglabel:K-tuple size |
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258 | argtype:slider |
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259 | argmin:1 |
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260 | argmax:4 |
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261 | argvalue:2 |
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262 | |
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263 | arg:TOPDIAGS |
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264 | arglabel:No. of top diagonals |
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265 | argtype:slider |
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266 | argmin:1 |
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267 | argmax:50 |
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268 | argvalue:4 |
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269 | |
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270 | arg:WINDOW |
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271 | arglabel:Window size |
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272 | argtype:slider |
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273 | argmin:1 |
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274 | argmax:50 |
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275 | argvalue:4 |
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276 | |
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277 | // parameters for multiple alignment: |
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278 | |
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279 | arg:DNAMATRIX |
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280 | argtype:choice_menu |
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281 | arglabel:Weighting matrix (multiple ali) |
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282 | argchoice:IUB:IUB |
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283 | argchoice:CLUSTALW:CLUSTALW |
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284 | |
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285 | arg:GOP |
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286 | argtype:slider |
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287 | arglabel:Gap Open Penalty |
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288 | argmin:0 |
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289 | argmax:100 |
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290 | argvalue:15 |
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291 | |
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292 | arg:GEP |
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293 | arglabel:Gap Extend Penalty |
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294 | argtype:slider |
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295 | argmin:0 |
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296 | argmax:10 |
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297 | argvalue:6.66 |
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298 | |
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299 | arg:MAXDIV |
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300 | arglabel:% identity for delay |
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301 | argtype:slider |
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302 | argmin:0 |
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303 | argmax:100 |
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304 | argvalue:30 |
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305 | |
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306 | arg:TRANSWEIGHT |
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307 | arglabel:Transition weight |
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308 | argtype:slider |
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309 | argmin:0.0 |
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310 | argmax:1.0 |
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311 | argvalue:0.5 |
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312 | |
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313 | arg:INTERACTIVE |
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314 | arglabel:Interactive |
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315 | argtype:choice_menu |
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316 | argchoice:No: |
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317 | argchoice:Yes:-interactive |
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318 | |
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319 | in:TmpInputFile |
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320 | informat:flat |
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321 | insave: |
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322 | |
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323 | out:TmpOutputFile |
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324 | outformat:flat |
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325 | |
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326 | // -------------------------------------------------------------------------------- |
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327 | |
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328 | item:ClustalW DNA Alignment (slow & accurate) |
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329 | seqtype: N |
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330 | itemmeta: s |
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331 | itemmethod:(GOTO_LOCAL_DIR; \ |
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332 | tr '"%//' '>' <TmpInputFile >clus_in ; \ |
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333 | RUN_IN_WINDOW((clustalw -output=PIR -infile=clus_in -align \ |
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334 | $INTERACTIVE \ |
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335 | -pwdnamatrix=$PWDNAMATRIX \ |
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336 | -pwgapopen=$PWGOP -pwgapext=$PWGEP \ |
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337 | -type=DNA -transweight=$TRANSWEIGHT \ |
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338 | -gapopen=$GOP -gapext=$GEP -maxdiv=$MAXDIV \ |
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339 | -dnamatrix=$DNAMATRIX; \ |
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340 | cat clus_in.pir | arb_sed "s/^>DL;/#/g" | sed "s/^>P1;/%/g" | arb_sed "s/^\*$//g" >TmpOutputFile; )) ; \ |
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341 | RM_LOCAL_FILES(clus_in* TmpInputFile)) |
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342 | |
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343 | itemhelp:clustalw.help |
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344 | |
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345 | // parameters for pairwise alignment: |
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346 | |
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347 | arg:PWDNAMATRIX |
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348 | argtype:choice_menu |
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349 | arglabel:Weighting matrix (pairwise ali) |
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350 | argchoice:IUB:IUB |
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351 | argchoice:CLUSTALW:CLUSTALW |
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352 | |
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353 | arg:PWGOP |
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354 | argtype:slider |
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355 | arglabel:Gap Open Penalty |
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356 | argmin:0 |
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357 | argmax:100 |
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358 | argvalue:15 |
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359 | |
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360 | arg:PWGEP |
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361 | arglabel:Gap Extend Penalty |
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362 | argtype:slider |
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363 | argmin:0 |
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364 | argmax:10 |
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365 | argvalue:6.66 |
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366 | |
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367 | // parameters for multiple alignment: |
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368 | |
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369 | arg:DNAMATRIX |
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370 | argtype:choice_menu |
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371 | arglabel:Weighting matrix (multiple ali) |
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372 | argchoice:IUB:IUB |
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373 | argchoice:CLUSTALW:CLUSTALW |
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374 | |
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375 | arg:GOP |
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376 | argtype:slider |
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377 | arglabel:Gap Open Penalty |
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378 | argmin:0 |
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379 | argmax:100 |
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380 | argvalue:15 |
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381 | |
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382 | arg:GEP |
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383 | arglabel:Gap Extend Penalty |
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384 | argtype:slider |
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385 | argmin:0 |
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386 | argmax:10 |
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387 | argvalue:6.66 |
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388 | |
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389 | arg:MAXDIV |
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390 | arglabel:% identity for delay |
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391 | argtype:slider |
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392 | argmin:0 |
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393 | argmax:100 |
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394 | argvalue:30 |
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395 | |
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396 | arg:TRANSWEIGHT |
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397 | arglabel:Transition weight |
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398 | argtype:slider |
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399 | argmin:0.0 |
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400 | argmax:1.0 |
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401 | argvalue:0.5 |
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402 | |
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403 | arg:INTERACTIVE |
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404 | arglabel:Interactive |
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405 | argtype:choice_menu |
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406 | argchoice:No: |
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407 | argchoice:Yes:-interactive |
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408 | |
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409 | in:TmpInputFile |
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410 | informat:flat |
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411 | insave: |
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412 | |
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413 | out:TmpOutputFile |
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414 | outformat:flat |
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415 | |
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416 | // -------------------------------------------------------------------------------- |
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417 | |
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418 | item:ClustalW Protein Alignment (fast) |
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419 | seqtype: A |
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420 | itemmeta: o |
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421 | itemmethod:(GOTO_LOCAL_DIR; \ |
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422 | tr '"%//' '>' <TmpInputFile >clus_in ; \ |
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423 | RUN_IN_WINDOW((clustalw -output=PIR -infile=clus_in -align \ |
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424 | -quicktree $INTERACTIVE \ |
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425 | -ktuple=$KTUPLE -topdiags=$TOPDIAGS \ |
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426 | -window=$WINDOW -pairgap=$PAIRGAP \ |
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427 | -score=$SCORE \ |
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428 | -type=PROTEIN -transweight=$TRANSWEIGHT \ |
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429 | -gapopen=$GOP -gapext=$GEP -maxdiv=$MAXDIV \ |
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430 | -matrix=$MATRIX $NEGMATRIX; \ |
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431 | cat clus_in.pir | arb_sed "s/^>DL;/#/g" | sed "s/^>P1;/%/g" | arb_sed "s/^\*$//g" >TmpOutputFile; )) ; \ |
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432 | RM_LOCAL_FILES(clus_in* TmpInputFile)) |
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433 | |
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434 | itemhelp:clustalw.help |
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435 | |
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436 | // parameters for fast pairwise alignment: |
---|
437 | |
---|
438 | arg:PAIRGAP |
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439 | arglabel:Gap penalty (pairwise ali) |
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440 | argtype:slider |
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441 | argmin:1 |
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442 | argmax:500 |
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443 | argvalue:3 |
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444 | |
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445 | arg:KTUPLE |
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446 | arglabel:K-tuple size |
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447 | argtype:slider |
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448 | argmin:1 |
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449 | argmax:2 |
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450 | argvalue:1 |
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451 | |
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452 | arg:TOPDIAGS |
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453 | arglabel:No. of top diagonals |
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454 | argtype:slider |
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455 | argmin:1 |
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456 | argmax:50 |
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457 | argvalue:5 |
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458 | |
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459 | arg:WINDOW |
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460 | arglabel:Window size |
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461 | argtype:slider |
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462 | argmin:1 |
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463 | argmax:50 |
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464 | argvalue:5 |
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465 | |
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466 | // parameters for multiple alignment: |
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467 | |
---|
468 | arg:MATRIX |
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469 | argtype:choice_menu |
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470 | arglabel:Weighting matrix (multiple ali) |
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471 | argchoice:Gonnet series:GONNET |
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472 | argchoice:Blosum series:BLOSUM |
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473 | argchoice:PAM series:PAM |
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474 | argchoice:ID matrix:ID |
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475 | |
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476 | arg:GOP |
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477 | argtype:slider |
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478 | arglabel:Gap Open Penalty |
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479 | argmin:0 |
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480 | argmax:100 |
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481 | argvalue:10 |
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482 | |
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483 | arg:GEP |
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484 | arglabel:Gap Extend Penalty |
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485 | argtype:slider |
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486 | argmin:0 |
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487 | argmax:10 |
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488 | argvalue:0.2 |
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489 | |
---|
490 | arg:MAXDIV |
---|
491 | arglabel:% identity for delay |
---|
492 | argtype:slider |
---|
493 | argmin:0 |
---|
494 | argmax:100 |
---|
495 | argvalue:30 |
---|
496 | |
---|
497 | arg:TRANSWEIGHT |
---|
498 | arglabel:Transition weight |
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499 | argtype:slider |
---|
500 | argmin:0.0 |
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501 | argmax:1.0 |
---|
502 | argvalue:0.5 |
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503 | |
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504 | arg:NEGMATRIX |
---|
505 | arglabel:Use negative matrix |
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506 | argtype:choice_menu |
---|
507 | argchoice:No: |
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508 | argchoice:Yes:-negative |
---|
509 | |
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510 | arg:INTERACTIVE |
---|
511 | arglabel:Interactive |
---|
512 | argtype:choice_menu |
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513 | argchoice:No: |
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514 | argchoice:Yes:-interactive |
---|
515 | |
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516 | in:TmpInputFile |
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517 | informat:flat |
---|
518 | insave: |
---|
519 | |
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520 | out:TmpOutputFile |
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521 | outformat:flat |
---|
522 | |
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523 | // -------------------------------------------------------------------------------- |
---|
524 | |
---|
525 | item:ClustalW Protein Alignment (slow & accurate) |
---|
526 | seqtype: A |
---|
527 | itemmeta: l |
---|
528 | itemmethod:(GOTO_LOCAL_DIR; \ |
---|
529 | tr '"%//' '>' <TmpInputFile >clus_in ; \ |
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530 | RUN_IN_WINDOW((clustalw -output=PIR -infile=clus_in -align \ |
---|
531 | $INTERACTIVE \ |
---|
532 | -pwmatrix=$PWMATRIX \ |
---|
533 | -pwgapopen=$PWGOP -pwgapext=$PWGEP \ |
---|
534 | -type=PROTEIN -transweight=$TRANSWEIGHT \ |
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535 | -gapopen=$GOP -gapext=$GEP -maxdiv=$MAXDIV \ |
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536 | -matrix=$MATRIX $NEGMATRIX; \ |
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537 | cat clus_in.pir | arb_sed "s/^>DL;/#/g" | sed "s/^>P1;/%/g" | arb_sed "s/^\*$//g" >TmpOutputFile; )) ; \ |
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538 | RM_LOCAL_FILES(clus_in* TmpInputFile)) |
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539 | |
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540 | itemhelp:clustalw.help |
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541 | |
---|
542 | // parameters for pairwise alignment: |
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543 | |
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544 | arg:PWMATRIX |
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545 | argtype:choice_menu |
---|
546 | arglabel:Weighting matrix (pairwise ali) |
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547 | argchoice:Gonnet series:GONNET |
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548 | argchoice:Blosum series:BLOSUM |
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549 | argchoice:PAM series:PAM |
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550 | argchoice:ID matrix:ID |
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551 | |
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552 | arg:PWGOP |
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553 | argtype:slider |
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554 | arglabel:Gap Open Penalty |
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555 | argmin:0 |
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556 | argmax:100 |
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557 | argvalue:10 |
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558 | |
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559 | arg:PWGEP |
---|
560 | arglabel:Gap Extend Penalty |
---|
561 | argtype:slider |
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562 | argmin:0 |
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563 | argmax:10 |
---|
564 | argvalue:0.1 |
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565 | |
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566 | // parameters for multiple alignment: |
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567 | |
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568 | arg:MATRIX |
---|
569 | argtype:choice_menu |
---|
570 | arglabel:Weighting matrix (multiple ali) |
---|
571 | argchoice:Gonnet series:GONNET |
---|
572 | argchoice:Blosum series:BLOSUM |
---|
573 | argchoice:PAM series:PAM |
---|
574 | argchoice:ID matrix:ID |
---|
575 | |
---|
576 | arg:GOP |
---|
577 | argtype:slider |
---|
578 | arglabel:Gap Open Penalty |
---|
579 | argmin:0 |
---|
580 | argmax:100 |
---|
581 | argvalue:10 |
---|
582 | |
---|
583 | arg:GEP |
---|
584 | arglabel:Gap Extend Penalty |
---|
585 | argtype:slider |
---|
586 | argmin:0 |
---|
587 | argmax:10 |
---|
588 | argvalue:0.2 |
---|
589 | |
---|
590 | arg:MAXDIV |
---|
591 | arglabel:% identity for delay |
---|
592 | argtype:slider |
---|
593 | argmin:0 |
---|
594 | argmax:100 |
---|
595 | argvalue:30 |
---|
596 | |
---|
597 | arg:TRANSWEIGHT |
---|
598 | arglabel:Transition weight |
---|
599 | argtype:slider |
---|
600 | argmin:0.0 |
---|
601 | argmax:1.0 |
---|
602 | argvalue:0.5 |
---|
603 | |
---|
604 | arg:NEGMATRIX |
---|
605 | arglabel:Use negative matrix |
---|
606 | argtype:choice_menu |
---|
607 | argchoice:No: |
---|
608 | argchoice:Yes:-negative |
---|
609 | |
---|
610 | arg:INTERACTIVE |
---|
611 | arglabel:Interactive |
---|
612 | argtype:choice_menu |
---|
613 | argchoice:No: |
---|
614 | argchoice:Yes:-interactive |
---|
615 | |
---|
616 | in:TmpInputFile |
---|
617 | informat:flat |
---|
618 | insave: |
---|
619 | |
---|
620 | out:TmpOutputFile |
---|
621 | outformat:flat |
---|
622 | |
---|
623 | // -------------------------------------------------------------------------------- |
---|
624 | |
---|
625 | item:Assemble Contigs |
---|
626 | itemmeta: g |
---|
627 | seqtype:* |
---|
628 | itemmethod: ( GOTO_LOCAL_DIR; \ |
---|
629 | tr '"%//' '>' <TmpInputFile > tr.out; \ |
---|
630 | CAP2 tr.out $OVERLAP $PMATCH > TmpOutputFile; \ |
---|
631 | RM_LOCAL_FILES(TmpInputFile tr.out) ) |
---|
632 | itemhelp:CAP2.help |
---|
633 | |
---|
634 | arg:OVERLAP |
---|
635 | argtype:slider |
---|
636 | arglabel:Minimum overlap? |
---|
637 | argmin:5 |
---|
638 | argmax:100 |
---|
639 | argvalue:20 |
---|
640 | |
---|
641 | arg:PMATCH |
---|
642 | argtype:slider |
---|
643 | arglabel:Percent match required within overlap |
---|
644 | argmin:25 |
---|
645 | argmax:100 |
---|
646 | argvalue:90 |
---|
647 | |
---|
648 | in:TmpInputFile |
---|
649 | informat:flat |
---|
650 | insave: |
---|
651 | |
---|
652 | out:TmpOutputFile |
---|
653 | outformat:gde |
---|
654 | |
---|