1 | /* |
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2 | * getseq.c Adachi, J. 1994.04.28 |
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3 | * Copyright (C) 1992, 1993 J. Adachi & M. Hasegawa, All rights reserved. |
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4 | */ |
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5 | |
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6 | #include "protml.h" |
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7 | #include "prot_tml.h" |
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8 | |
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9 | void |
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10 | getsize(ifp, maxspc, numsite, commentp) |
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11 | FILE *ifp; |
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12 | int *maxspc; |
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13 | int *numsite; |
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14 | char **commentp; |
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15 | { |
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16 | char *cp, *np; |
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17 | char line[BUFLINE]; |
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18 | |
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19 | if (fgets(line, BUFLINE, ifp) != NULL) { |
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20 | if (sscanf(line, "%d %d", maxspc, numsite) == 2) { |
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21 | for (cp = line; isdigit(*cp) || isspace(*cp) /*&& *cp != '\0'*/; cp++) |
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22 | ; |
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23 | *commentp = new_cvector(strlen(cp) + 1); |
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24 | if (*cp != '\0') { |
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25 | for (np = *commentp; *cp != '\n' && *cp != '\0'; cp++) |
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26 | *np++ = *cp; |
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27 | *np = '\0'; |
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28 | } else { |
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29 | **commentp = '\0'; |
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30 | } |
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31 | if (Debug) |
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32 | fprintf(stdout, "%d OTUs, %d sites, %s\n\n", |
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33 | *maxspc, *numsite, *commentp); |
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34 | } else { |
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35 | fputs(line, stderr); |
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36 | fprintf(stderr, "\nBad format, first line of input file.\n"); |
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37 | exit(1); |
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38 | } |
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39 | } else { |
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40 | fprintf(stderr, "\nCan't read input file.\n"); |
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41 | exit(1); |
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42 | } |
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43 | return; |
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44 | } /*_ getsize */ |
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45 | |
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46 | |
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47 | /* getid */ |
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48 | void |
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49 | getid(ifp, identif, sciname, engname, nl, notu) |
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50 | FILE *ifp; |
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51 | char **identif; |
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52 | char **sciname; |
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53 | char **engname; |
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54 | int *nl; |
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55 | int *notu; |
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56 | { |
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57 | int i; |
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58 | char line[BUFLINE]; |
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59 | char idbuf[BUFLINE]; |
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60 | char scibuf[BUFLINE]; |
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61 | char engbuf[BUFLINE]; |
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62 | char *cp, *np; |
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63 | |
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64 | while (fgets(line, BUFLINE, ifp) != NULL) { |
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65 | (*nl)++; /* line counter */ |
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66 | if (line[0] == '#') /* comment line skip */ |
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67 | continue; |
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68 | for (cp = line; isspace(*cp); cp++) /* white line skip */ |
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69 | ; |
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70 | if (*cp == '\0') |
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71 | continue; |
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72 | for (np = idbuf; !isspace(*cp); *np++ = *cp++) /* identifier */ |
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73 | ; |
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74 | *np = '\0'; |
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75 | for (i = 0; i < *notu; i++) { /* ID check */ |
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76 | if (strcmp(idbuf, identif[i]) == 0) { |
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77 | fprintf(stderr,"Identifier \"%s\" is double defined\n",idbuf); |
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78 | exit(1); |
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79 | } |
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80 | } |
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81 | identif[*notu] = |
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82 | (char *)malloc((unsigned)(strlen(idbuf) + 1) * sizeof(char)); |
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83 | if (identif[*notu] == NULL) maerror("in getid, identif"); |
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84 | strcpy(identif[*notu], idbuf); |
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85 | |
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86 | for ( ; isspace(*cp); cp++) /* white char skip */ |
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87 | ; |
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88 | /* science name */ |
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89 | if (*cp != '(' && *cp != '\0' && *cp != '#' && *cp != '\n') { |
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90 | for (np = scibuf; *cp!='(' && *cp!='#' && *cp!='\0' && *cp!='\n'; *np++ = *cp++) |
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91 | ; |
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92 | for ( ; isspace(*(np-1)); np--) |
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93 | ; |
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94 | *np = '\0'; |
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95 | } else { |
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96 | scibuf[0] = '\0'; |
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97 | } |
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98 | sciname[*notu] = |
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99 | (char *)malloc((unsigned)(strlen(scibuf) + 1) * sizeof(char)); |
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100 | if (identif[*notu] == NULL) maerror("in getid, sciname"); |
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101 | strcpy(sciname[*notu], scibuf); |
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102 | |
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103 | /* english name */ |
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104 | if (*cp != '\0' && *cp != '#' && *cp != '\n') { |
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105 | for (np = engbuf; *cp!='#' && *cp!='\0' && *cp!='\n'; *np++ = *cp++) |
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106 | ; |
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107 | for ( ; isspace(*(np-1)); np--) |
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108 | ; |
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109 | *np = '\0'; |
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110 | } else { |
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111 | engbuf[0] = '\0'; |
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112 | } |
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113 | engname[*notu] = |
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114 | (char *)malloc((unsigned)(strlen(engbuf) + 1) * sizeof(char)); |
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115 | if (identif[*notu] == NULL) maerror("in getid, engname"); |
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116 | strcpy(engname[*notu], engbuf); |
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117 | |
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118 | if (Debug) { |
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119 | printf("%3d OTU \"%s\"", *notu+1, identif[*notu]); |
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120 | if (sciname[*notu] != '\0') { |
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121 | fputs(" sci:", stdout); |
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122 | fputs(sciname[*notu], stdout); |
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123 | } |
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124 | if (engname[*notu] != '\0') { |
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125 | putchar(' '); |
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126 | fputs(" eng:", stdout); |
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127 | fputs(engname[*notu], stdout); |
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128 | } |
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129 | putchar('\n'); |
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130 | } |
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131 | break; |
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132 | } |
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133 | return; |
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134 | } /*_ getid */ |
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135 | |
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136 | |
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137 | /* getsites */ |
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138 | void |
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139 | getsites(ifp, seqchar, numsite, nl, notu) |
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140 | FILE *ifp; |
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141 | cmatrix seqchar; |
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142 | int numsite, *nl, *notu; |
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143 | { |
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144 | char line[BUFLINE]; |
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145 | char c; |
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146 | int i, imax, nsite; |
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147 | |
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148 | nsite = 0; |
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149 | while (fgets(line, BUFLINE, ifp) != NULL) { |
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150 | (*nl)++; /* line counter */ |
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151 | if (line[0] == '#') /* comment line skip */ |
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152 | continue; |
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153 | for (i = 0, imax = strlen(line); i < imax && nsite < numsite; i++) { |
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154 | c = toupper(line[i]); |
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155 | if (isacid(c)) { |
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156 | seqchar[*notu][nsite++] = acid2int(c); |
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157 | } else if (isspace(c)) { |
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158 | /* skip white char */ |
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159 | } else { |
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160 | fputs(line, stderr); |
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161 | fprintf(stderr, "Expected %s Acid Character: '%c'\n", |
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162 | Infomol, c); |
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163 | fprintf(stderr, "%d line %d column, %d OTU %d site\n", |
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164 | *nl+1, i+1, *notu+1, nsite+1); |
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165 | exit(1); |
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166 | } |
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167 | } |
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168 | if (nsite >= numsite) { |
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169 | if (i != imax && !isspace(c = toupper(line[i])) && c != '\0') { |
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170 | fputs(line, stderr); |
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171 | fprintf(stderr, "Inconsistent size of sequence: '%c'\n", c); |
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172 | fprintf(stderr, "%d line %d column, %d OTU %d site\n", |
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173 | *nl+1, i+1, *notu+1, nsite+1); |
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174 | exit(1); |
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175 | } |
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176 | break; |
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177 | } |
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178 | } |
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179 | return; |
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180 | } /* getsites */ |
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181 | |
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182 | |
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183 | void getseq(ifp, identif, sciname, engname, seqchar, maxspc, numsite) |
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184 | FILE *ifp; |
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185 | char **identif, **sciname, **engname; |
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186 | cmatrix seqchar; |
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187 | int maxspc, numsite; |
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188 | { |
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189 | int notu; |
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190 | int nl = 0; |
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191 | |
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192 | for (notu = 0; notu < maxspc; notu++) { |
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193 | getid(ifp, identif, sciname, engname, &nl, ¬u); |
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194 | getsites(ifp, seqchar, numsite, &nl, ¬u); |
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195 | } |
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196 | return; |
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197 | } /*_ getseq */ |
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198 | |
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199 | |
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200 | /* getidsites */ |
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201 | void |
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202 | getidsites(ifp, identif, seqchar, numsite, nl, notu) |
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203 | FILE *ifp; |
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204 | cmatrix identif, seqchar; |
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205 | int numsite, *nl, *notu; |
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206 | { |
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207 | char line[BUFLINE]; |
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208 | char idbuf[BUFLINE]; |
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209 | char c; |
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210 | int i, imax, nsite; |
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211 | |
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212 | nsite = 0; |
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213 | while (fgets(line, BUFLINE, ifp) != NULL) { |
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214 | (*nl)++; /* line counter */ |
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215 | if (line[0] == '#') /* comment line skip */ |
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216 | continue; |
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217 | if (nsite == 0) { |
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218 | strncpy(idbuf, line, NMLNGTH); |
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219 | i = NMLNGTH; |
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220 | while (isspace(idbuf[--i])) |
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221 | ; |
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222 | idbuf[++i] = '\0'; |
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223 | for (i = 0; i < *notu; i++) { /* ID check */ |
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224 | if (strcmp(idbuf, identif[i]) == 0) { |
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225 | fprintf(stderr, |
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226 | "Identifier \"%s\" is double defined\n",idbuf); |
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227 | exit(1); |
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228 | } |
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229 | } |
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230 | identif[*notu] = |
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231 | (char *)malloc((unsigned)(strlen(idbuf) + 1) * sizeof(char)); |
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232 | if (identif[*notu] == NULL) maerror("in getidsites, identif"); |
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233 | strcpy(identif[*notu], idbuf); |
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234 | for (i=NMLNGTH, imax=strlen(line); i<imax && nsite<numsite; i++) { |
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235 | c = toupper(line[i]); |
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236 | if (isacid(c)) { |
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237 | seqchar[*notu][nsite++] = acid2int(c); |
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238 | } else if (isspace(c)) { |
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239 | /* skip white char */ |
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240 | } else { |
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241 | fputs(line, stderr); |
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242 | fprintf(stderr, "Expected %s Acid Character: '%c'\n", |
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243 | Infomol, c); |
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244 | fprintf(stderr, "%d line %d column, %d OTU %d site\n", |
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245 | *nl+1, i+1, *notu+1, nsite+1); |
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246 | exit(1); |
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247 | } |
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248 | } |
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249 | } else { |
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250 | for (i=0, imax=strlen(line); i < imax && nsite < numsite; i++) { |
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251 | c = toupper(line[i]); |
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252 | if (isacid(c)) { |
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253 | seqchar[*notu][nsite++] = acid2int(c); |
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254 | } else if (isspace(c)) { |
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255 | /* skip white char */ |
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256 | } else { |
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257 | fputs(line, stderr); |
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258 | fprintf(stderr, "Expected %s Acid Character: '%c'\n", |
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259 | Infomol, c); |
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260 | fprintf(stderr, "%d line %d column, %d OTU %d site\n", |
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261 | *nl+1, i+1, *notu+1, nsite+1); |
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262 | exit(1); |
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263 | } |
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264 | } |
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265 | } |
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266 | if (nsite >= numsite) { |
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267 | if (i != imax && !isspace(c = toupper(line[i])) && c != '\0') { |
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268 | fputs(line, stderr); |
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269 | fprintf(stderr, "Inconsistent size of sequence: '%c'\n", c); |
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270 | fprintf(stderr, "%d line %d column, %d OTU %d site\n", |
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271 | *nl+1, i+1, *notu+1, nsite+1); |
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272 | exit(1); |
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273 | } |
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274 | break; |
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275 | } |
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276 | } |
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277 | return; |
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278 | } |
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279 | /*_ getidsites */ |
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280 | |
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281 | |
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282 | void getseqs(ifp, identif, seqchar, maxspc, numsite) |
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283 | FILE *ifp; |
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284 | char **identif; |
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285 | cmatrix seqchar; |
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286 | int maxspc, numsite; |
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287 | { |
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288 | int notu; |
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289 | int nl = 0; |
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290 | |
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291 | for (notu = 0; notu < maxspc; notu++) |
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292 | getidsites(ifp, identif, seqchar, numsite, &nl, ¬u); |
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293 | return; |
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294 | } /*_ getseqs */ |
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295 | |
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296 | |
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297 | void getseqi(ifp, identif, seqchar, maxspc, numsite) |
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298 | FILE *ifp; |
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299 | char **identif; |
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300 | cmatrix seqchar; |
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301 | int maxspc, numsite; |
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302 | { |
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303 | int notu, nl, nsite, ns, i, imax; |
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304 | char line[BUFLINE]; |
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305 | char idbuf[BUFLINE]; |
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306 | char c, *cp; |
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307 | |
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308 | nsite = 0; |
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309 | nl = 0; |
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310 | while (nsite < numsite) { |
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311 | for (notu = 0; notu < maxspc; notu++) { |
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312 | while (fgets(line, BUFLINE, ifp) != NULL) { |
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313 | nl++; /* line counter */ |
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314 | for (cp = line; isspace(*cp); cp++) /* white line skip */ |
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315 | ; |
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316 | if (*cp == '\0') |
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317 | continue; |
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318 | if (nsite == 0) { |
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319 | strncpy(idbuf, line, NMLNGTH); |
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320 | i = NMLNGTH; |
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321 | while (isspace(idbuf[--i])) |
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322 | ; |
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323 | idbuf[++i] = '\0'; |
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324 | for (i = 0; i < notu; i++) { /* ID check */ |
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325 | if (strcmp(idbuf, identif[i]) == 0) { |
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326 | fprintf(stderr, |
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327 | "Identifier \"%s\" is double defined\n",idbuf); |
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328 | exit(1); |
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329 | } |
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330 | } |
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331 | identif[notu] = |
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332 | (char *)malloc((unsigned)(strlen(idbuf)+1) * sizeof(char)); |
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333 | if (identif[notu] == NULL) maerror("in getidsites, identif"); |
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334 | strcpy(identif[notu], idbuf); |
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335 | i = NMLNGTH; |
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336 | } else { |
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337 | i = 0; |
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338 | } |
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339 | for (ns=nsite, imax=strlen(line); i<imax && ns<numsite; i++) { |
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340 | c = toupper(line[i]); |
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341 | if (isacid(c)) { |
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342 | seqchar[notu][ns++] = acid2int(c); |
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343 | } else if (isspace(c)) { |
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344 | /* skip white char */ |
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345 | } else { |
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346 | fputs(line, stderr); |
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347 | fprintf(stderr, "Expected %s Acid Character: '%c'\n", |
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348 | Infomol, c); |
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349 | fprintf(stderr,"%d line %d column, %d OTU %d site\n", |
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350 | nl+1, i+1, notu+1, ns+1); |
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351 | exit(1); |
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352 | } |
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353 | } |
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354 | if (notu == maxspc-1) nsite = ns; |
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355 | if (nsite >= numsite) { |
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356 | if (i != imax && !isspace(c = toupper(line[i])) && c != '\0') { |
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357 | fputs(line, stderr); |
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358 | fprintf(stderr, "Inconsistent size of sequence: '%c'\n", c); |
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359 | fprintf(stderr, "%d line %d column, %d OTU %d site\n", |
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360 | nl+1, i+1, notu+1, nsite+1); |
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361 | exit(1); |
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362 | } |
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363 | break; |
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364 | } |
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365 | break; |
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366 | } |
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367 | } |
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368 | } |
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369 | return; |
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370 | |
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371 | } /*_ getseqi */ |
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372 | |
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373 | |
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374 | /* put identifier of otu */ |
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375 | void |
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376 | fputid(ofp, name, maxcolumn) |
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377 | FILE *ofp; |
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378 | char *name; |
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379 | int maxcolumn; |
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380 | { |
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381 | int i, imax; |
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382 | |
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383 | if ((imax = strlen(name)) < maxcolumn) { |
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384 | fputs(name, ofp); |
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385 | for (i = imax; i < maxcolumn; i++) |
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386 | fputc(' ', ofp); |
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387 | } else { |
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388 | for (i = 0; i < maxcolumn; i++) |
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389 | fputc(name[i], ofp); |
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390 | } |
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391 | } |
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392 | |
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393 | |
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394 | /* print sequences */ |
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395 | void |
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396 | prsequence(ofp, identif, seqchar, maxspc, maxsite) |
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397 | FILE *ofp; |
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398 | char **identif; |
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399 | char **seqchar; |
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400 | int maxspc, maxsite; |
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401 | { |
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402 | int i, nst, maxst, nsp; |
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403 | char ch; |
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404 | ivector counti; |
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405 | cvector consenseq; |
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406 | |
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407 | counti = new_ivector(Tpmradix + 1); |
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408 | consenseq = new_cvector(maxsite); |
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409 | for (nst = 0; nst < maxsite; nst++) { /* make consensus sequence */ |
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410 | for (i = 0; i < Tpmradix + 1; i++) counti[i] = 0; |
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411 | for (nsp = 0; nsp < maxspc; nsp++) { /* count char */ |
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412 | counti[ seqchar[nsp][nst] ]++; |
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413 | } |
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414 | consenseq[nst] = '.'; |
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415 | for (i = 0; i < Tpmradix; i++) { /* + 1 */ |
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416 | if (counti[i] > maxspc / 2) { |
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417 | consenseq[nst] = int2acid(i); |
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418 | break; |
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419 | } |
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420 | } |
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421 | } |
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422 | |
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423 | fputc('\n', ofp); |
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424 | for (i = 0; i < maxsite; i += Linesites) { |
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425 | maxst = (i + Linesites < maxsite) ? (i + Linesites) : maxsite; |
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426 | fputid(ofp, "CONSENSUS", 10); |
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427 | for (nst = i; nst < maxst; nst++) { |
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428 | if (nst % 10 == 0) fputc(' ', ofp); |
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429 | fputc(consenseq[nst], ofp); |
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430 | } |
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431 | fputc('\n', ofp); |
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432 | for (nsp = 0; nsp < maxspc; nsp++) { |
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433 | fputid(ofp, identif[nsp], 10); |
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434 | for (nst = i; nst < maxst; nst++) { |
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435 | if (nst % 10 == 0) fputc(' ', ofp); |
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436 | ch = chint2acid(seqchar[nsp][nst]); |
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437 | if (ch != consenseq[nst]) |
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438 | fputc(ch, ofp); |
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439 | else |
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440 | fputc('.', ofp); |
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441 | } |
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442 | fputc('\n', ofp); |
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443 | } |
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444 | fputid(ofp, "", 10); |
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445 | for (nst = i; nst < maxst; nst+=10) { |
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446 | if (nst+10 <= maxst) printf(" %10d", nst+10); |
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447 | } |
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448 | fputc('\n', ofp); |
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449 | } |
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450 | free_cvector(consenseq); |
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451 | free_ivector(counti); |
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452 | return; |
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453 | } /*_ prsequence */ |
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