| 1 | #Please insert up references in the next lines (line starts with keyword UP) |
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| 2 | UP arb.hlp |
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| 3 | UP glossary.hlp |
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| 4 | UP mark.hlp |
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| 5 | |
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| 6 | #Please insert subtopic references (line starts with keyword SUB) |
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| 7 | #SUB nt_align_select.hlp |
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| 8 | |
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| 9 | # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} |
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| 10 | |
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| 11 | #************* Title of helpfile !! and start of real helpfile ******** |
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| 12 | TITLE How to get an initial tree |
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| 13 | |
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| 14 | DESCRIPTION The main idea of ARB is to manage database access via a tree. |
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| 15 | |
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| 16 | [ You don't have a tree, but you may access all |
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| 17 | species data using 'Species/Search and Query'. ] |
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| 18 | |
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| 19 | There are several ways to construct an initial tree: |
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| 20 | |
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| 21 | Align the sequence data: |
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| 22 | |
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| 23 | 1. Mark all 'species' (see LINK{glossary.hlp}) |
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| 24 | Choose the item 'Mark all Species' |
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| 25 | from the menu 'Species' |
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| 26 | of the 'ARB_NT' main window (this program) |
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| 27 | (normally this is referred as: 'ARB_NT/Species/Mark all Species'); |
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| 28 | |
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| 29 | 2. Select an alignment: |
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| 30 | <ARB_NT/3rd big Button in top area> |
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| 31 | (see LINK{nt_align_select.hlp}) |
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| 32 | |
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| 33 | 3. To align the marked sequences use one of the provided aligners |
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| 34 | in 'ARB_NT/Sequence/Align Sequences' |
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| 35 | |
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| 36 | Reconstruct an initial tree: |
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| 37 | |
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| 38 | To get a good tree, you should use different treeing |
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| 39 | methods. |
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| 40 | |
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| 41 | To quickly get an initial tree we recommend to use |
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| 42 | neighbour joining for DNA, RNA and Protein sequences: |
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| 43 | |
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| 44 | To start neighbour joining select 'ARB_NT/Tree/Build tree from sequence data/Distance matrix methods/ARB Neighbor Joining', |
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| 45 | to display the 'NEIGHBOUR JOINING [ARB_DIST]' window. |
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| 46 | Refer to LINK{dist.hlp} for setting parameters of tree reconstruction. |
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| 47 | |
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| 48 | Show the reconstructed tree: |
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| 49 | |
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| 50 | Click on the <tree_*> (2nd big rectangular) button in |
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| 51 | top area of ARB_NT and choose the respective tree. |
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| 52 | |
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| 53 | Save everything: |
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| 54 | |
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| 55 | Choose the 'Save Whole Database as' item from the 'File' menu. |
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| 56 | |
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| 57 | NOTES None |
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| 58 | |
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| 59 | WARNINGS None |
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| 60 | |
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| 61 | BUGS No bugs known |
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