1 | // =============================================================== // |
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2 | // // |
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3 | // File : graph_aligner_gui.cxx // |
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4 | // Purpose : // |
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5 | // // |
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6 | // Coded by Elmar Pruesse in October 2008 // |
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7 | // Institute of Microbiology (Technical University Munich) // |
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8 | // http://www.arb-home.de/ // |
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9 | // // |
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10 | // =============================================================== // |
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11 | |
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12 | #include "graph_aligner_gui.hxx" |
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13 | |
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14 | #include "ed4_defs.hxx" |
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15 | |
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16 | // need to use AlignDataAccess defined here to get selected species |
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17 | #include <fast_aligner.hxx> |
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18 | |
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19 | #include <aw_awars.hxx> |
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20 | #include <aw_global_awars.hxx> |
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21 | #include <aw_msg.hxx> |
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22 | #include <aw_root.hxx> |
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23 | #include <awt_sel_boxes.hxx> |
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24 | #include <awt_config_manager.hxx> |
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25 | #include <servercntrl.h> |
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26 | #include <PT_com.h> |
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27 | #include <client.h> |
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28 | #include <arbdbt.h> |
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29 | #include <arb_strbuf.h> |
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30 | #include <arb_misc.h> |
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31 | #include <arb_file.h> |
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32 | |
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33 | #include <sys/types.h> |
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34 | #include <sys/wait.h> |
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35 | |
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36 | #include <unistd.h> |
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37 | |
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38 | #include <string> |
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39 | #include <sstream> |
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40 | #include <iostream> |
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41 | #include <vector> |
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42 | |
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43 | using std::cerr; |
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44 | using std::cout; |
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45 | using std::endl; |
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46 | |
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47 | #define ga_assert(cond) arb_assert(cond) |
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48 | |
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49 | #define GA_AWAR_ROOT "sina/" |
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50 | #define GA_AWAR_TEMP "tmp/" GA_AWAR_ROOT |
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51 | |
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52 | #define GA_AWAR_CMD GA_AWAR_ROOT "cmd" |
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53 | #define GA_AWAR_TGT GA_AWAR_ROOT "target" |
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54 | #define GA_AWAR_SAI GA_AWAR_ROOT "sai" |
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55 | #define GA_AWAR_ALIGNMENT GA_AWAR_ROOT "alignment" |
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56 | #define GA_AWAR_PROTECTION GA_AWAR_ROOT "protection" |
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57 | #define GA_AWAR_TURN_CHECK GA_AWAR_ROOT "turncheck" |
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58 | #define GA_AWAR_REALIGN GA_AWAR_ROOT "realign" |
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59 | #define GA_AWAR_MATCH_SCORE GA_AWAR_ROOT "match_score" |
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60 | #define GA_AWAR_MISMATCH_SCORE GA_AWAR_ROOT "mismatch_score" |
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61 | #define GA_AWAR_GAP_PEN GA_AWAR_ROOT "gap_pen" |
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62 | #define GA_AWAR_GAP_EXT GA_AWAR_ROOT "gap_ext" |
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63 | #define GA_AWAR_ADVANCED GA_AWAR_ROOT "advanced" |
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64 | #define GA_AWAR_FS_MIN GA_AWAR_ROOT "fs_min" |
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65 | #define GA_AWAR_FS_MAX GA_AWAR_ROOT "fs_max" |
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66 | #define GA_AWAR_FS_MSC GA_AWAR_ROOT "fs_msc" |
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67 | #define GA_AWAR_MIN_FULL GA_AWAR_ROOT "min_full" |
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68 | #define GA_AWAR_FULL_MINLEN GA_AWAR_ROOT "full_minlen" |
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69 | #define GA_AWAR_OVERHANG GA_AWAR_ROOT "overhang" |
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70 | #define GA_AWAR_THREADS GA_AWAR_ROOT "threads" |
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71 | #define GA_AWAR_QSIZE GA_AWAR_ROOT "qsize" |
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72 | #define GA_AWAR_KMER_LEN GA_AWAR_ROOT "kmer_len" |
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73 | #define GA_AWAR_KMER_MM GA_AWAR_ROOT "kmer_mm" |
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74 | #define GA_AWAR_MIN_LEN GA_AWAR_ROOT "min_len" |
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75 | #define GA_AWAR_WEIGHT GA_AWAR_ROOT "weight" |
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76 | #define GA_AWAR_INSERT GA_AWAR_ROOT "insert" |
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77 | #define GA_AWAR_LOWERCASE GA_AWAR_ROOT "lowercase" |
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78 | #define GA_AWAR_AUTOFILTER GA_AWAR_ROOT "autofilter" |
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79 | #define GA_AWAR_KMER_NOREL GA_AWAR_ROOT "kmer_norel" |
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80 | #define GA_AWAR_KMER_NOFAST GA_AWAR_ROOT "kmer_nofast" |
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81 | #define GA_AWAR_SHOW_DIST GA_AWAR_ROOT "show_dist" |
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82 | #define GA_AWAR_SHOW_DIFF GA_AWAR_ROOT "show_diff" |
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83 | #define GA_AWAR_COLOR GA_AWAR_ROOT "color" |
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84 | #define GA_AWAR_GENE_START GA_AWAR_ROOT "gene_start" |
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85 | #define GA_AWAR_GENE_END GA_AWAR_ROOT "gene_end" |
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86 | #define GA_AWAR_FS_COVER_GENE GA_AWAR_ROOT "fs_cover_gene" |
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87 | #define GA_AWAR_USED_RELS GA_AWAR_ROOT "write_used_rels" |
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88 | #define GA_AWAR_CALC_IDENTITY GA_AWAR_ROOT "calc_idty" |
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89 | #define GA_AWAR_NOWARN_START GA_AWAR_ROOT "nowarn_start" |
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90 | #define GA_AWAR_REFDB_MODE GA_AWAR_ROOT "refdb_mode" |
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91 | #define GA_AWAR_REFDB GA_AWAR_ROOT "refdb" |
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92 | #define GA_AWAR_REFDB_SELECT GA_AWAR_TEMP "refdb" |
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93 | |
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94 | enum RefDbMode { |
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95 | REF_EXPLICIT, // use explicitly specified database |
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96 | REF_LAST_SAVE, // use last saved state of loaded database |
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97 | REF_CURRENT, // use current state of loaded database |
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98 | }; |
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99 | |
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100 | static void refDbModeChanged_cb(AW_root *root) { |
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101 | RefDbMode mode = RefDbMode(root->awar(GA_AWAR_REFDB_MODE)->read_int()); |
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102 | AW_awar *awar_refdb = root->awar(GA_AWAR_REFDB); |
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103 | |
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104 | switch (mode) { |
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105 | case REF_EXPLICIT: { |
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106 | const char *refdb = awar_refdb->read_char_pntr(); |
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107 | if (!GB_is_readablefile(refdb)) { |
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108 | const char *refdb_sel = root->awar(GA_AWAR_REFDB_SELECT)->read_char_pntr(); |
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109 | if (GB_is_readablefile(refdb_sel)) { |
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110 | awar_refdb->write_string(refdb_sel); |
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111 | } |
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112 | else { |
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113 | aw_message("Please select a reference database"); |
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114 | } |
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115 | } |
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116 | break; |
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117 | } |
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118 | case REF_LAST_SAVE: { |
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119 | const char *db_name = root->awar(AWAR_SAVED_DB_PATH)->read_char_pntr(); |
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120 | if (db_name[0] == 0) { // happens when editor runs standalone |
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121 | aw_message("Failed to detect name of current database - please select manually"); |
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122 | } |
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123 | awar_refdb->write_string(db_name); |
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124 | break; |
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125 | } |
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126 | case REF_CURRENT: { |
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127 | awar_refdb->write_string(":"); |
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128 | break; |
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129 | } |
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130 | } |
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131 | } |
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132 | |
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133 | static void newRefDbSelected_cb(AW_root *root) { |
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134 | const char *refdb_sel = root->awar(GA_AWAR_REFDB_SELECT)->read_char_pntr(); |
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135 | |
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136 | if (GB_is_readablefile(refdb_sel)) { |
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137 | root->awar(GA_AWAR_REFDB_MODE)->write_int(REF_EXPLICIT); |
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138 | root->awar(GA_AWAR_REFDB)->write_string(refdb_sel); |
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139 | } |
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140 | else { |
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141 | root->awar(GA_AWAR_REFDB_MODE)->write_int(REF_CURRENT); |
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142 | } |
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143 | } |
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144 | |
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145 | void create_sina_variables(AW_root *root, AW_default db1) { |
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146 | const char *sinaCmd = "arb_sina.sh"; |
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147 | root->awar_string(GA_AWAR_CMD, sinaCmd, db1); |
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148 | root->awar_int (GA_AWAR_TGT, 2, db1); |
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149 | root->awar_int (AWAR_PT_SERVER, 0, db1); |
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150 | root->awar_string(GA_AWAR_SAI, "", db1); |
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151 | root->awar_int (GA_AWAR_PROTECTION, 0, db1); |
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152 | root->awar_int (GA_AWAR_TURN_CHECK, 1, db1); |
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153 | root->awar_int (GA_AWAR_REALIGN, 1, db1); |
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154 | root->awar_float (GA_AWAR_GAP_PEN, 5.0, db1); |
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155 | root->awar_float (GA_AWAR_GAP_EXT, 2.0, db1); |
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156 | root->awar_float (GA_AWAR_MATCH_SCORE, 2.0, db1); |
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157 | root->awar_float (GA_AWAR_MISMATCH_SCORE, -1.0, db1); |
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158 | root->awar_int (GA_AWAR_ADVANCED, 0, db1); |
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159 | root->awar_int (GA_AWAR_FS_MIN, 40, db1); |
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160 | root->awar_int (GA_AWAR_FS_MAX, 40, db1); |
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161 | root->awar_float (GA_AWAR_FS_MSC, .7, db1); |
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162 | root->awar_int (GA_AWAR_MIN_FULL, 1, db1); |
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163 | root->awar_int (GA_AWAR_FULL_MINLEN, 1400, db1); |
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164 | root->awar_string(GA_AWAR_OVERHANG, "attach", db1); |
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165 | root->awar_int (GA_AWAR_THREADS, 1, db1); |
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166 | root->awar_int (GA_AWAR_QSIZE, 1, db1); |
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167 | root->awar_int (GA_AWAR_KMER_LEN, 10, db1); |
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168 | root->awar_int (GA_AWAR_KMER_MM, 0, db1); |
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169 | root->awar_int (GA_AWAR_MIN_LEN, 150, db1); |
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170 | root->awar_float (GA_AWAR_WEIGHT, 1, db1); |
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171 | root->awar_string(GA_AWAR_INSERT, "shift", db1); |
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172 | root->awar_string(GA_AWAR_LOWERCASE, "none", db1); |
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173 | root->awar_string(GA_AWAR_AUTOFILTER, "", db1); |
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174 | root->awar_int (GA_AWAR_KMER_NOREL, 0, db1); |
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175 | root->awar_int (GA_AWAR_KMER_NOFAST, 0, db1); |
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176 | root->awar_int (GA_AWAR_SHOW_DIST, 0, db1); |
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177 | root->awar_int (GA_AWAR_SHOW_DIFF, 0, db1); |
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178 | root->awar_int (GA_AWAR_COLOR, 1, db1); |
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179 | root->awar_int (GA_AWAR_GENE_START, 0, db1); |
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180 | root->awar_int (GA_AWAR_GENE_END, 0, db1); |
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181 | root->awar_int (GA_AWAR_FS_COVER_GENE, 0, db1); |
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182 | root->awar_int (GA_AWAR_USED_RELS, 0, db1); |
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183 | root->awar_int (GA_AWAR_CALC_IDENTITY, 0, db1); |
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184 | root->awar_int (GA_AWAR_NOWARN_START, 0, db1); |
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185 | |
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186 | AW_awar *awar_mode = root->awar_int (GA_AWAR_REFDB_MODE, REF_CURRENT, db1); |
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187 | root->awar_string (GA_AWAR_REFDB, ":", db1); |
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188 | AW_awar *awar_select = root->awar_string(GA_AWAR_REFDB_SELECT, "", db1); |
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189 | |
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190 | awar_mode->add_callback(makeRootCallback(refDbModeChanged_cb)); |
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191 | awar_select->add_callback(makeRootCallback(newRefDbSelected_cb)); |
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192 | } |
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193 | |
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194 | enum SinaArbInterfaceVersion { |
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195 | ARB_5_99, |
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196 | ARB_7_1, |
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197 | }; |
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198 | const SinaArbInterfaceVersion NO_INTERFACE = SinaArbInterfaceVersion(-1); |
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199 | |
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200 | struct sina_arb_interface_def { |
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201 | SinaArbInterfaceVersion version; |
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202 | const char *id; // as define by SINA |
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203 | }; |
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204 | |
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205 | static sina_arb_interface_def supported_sina_versions[] = { |
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206 | // order matters: first supported version will be used. |
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207 | |
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208 | { ARB_7_1, "ARB7.1" }, // only defined in patched version delivered with arb |
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209 | { ARB_5_99, "ARB5.99" }, // supported at least by sina 1.3 up to 1.7.2 |
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210 | |
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211 | { NO_INTERFACE, "<none>" }, // version=sentinel |
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212 | }; |
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213 | |
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214 | static const char *get_interface_id(SinaArbInterfaceVersion version) { |
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215 | for (int v = 0; ; ++v) { |
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216 | const sina_arb_interface_def& sina_interface = supported_sina_versions[v]; |
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217 | if (sina_interface.version == version) { |
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218 | return sina_interface.id; |
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219 | } |
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220 | } |
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221 | return get_interface_id(NO_INTERFACE); |
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222 | } |
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223 | |
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224 | static SinaArbInterfaceVersion used_sina_interface = NO_INTERFACE; |
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225 | |
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226 | static GB_ERROR detect_sina_version(AW_root *awr) { |
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227 | const char *sinaName = awr->awar(GA_AWAR_CMD)->read_char_pntr(); |
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228 | char *sina = ARB_executable(sinaName, GB_getenv("PATH")); |
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229 | const char *fail_reason = NULp; |
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230 | std::string tested_interfaces = ""; |
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231 | |
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232 | used_sina_interface = NO_INTERFACE; |
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233 | |
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234 | if (sina) { |
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235 | if (ARB_path_contains_unwanted_chars(sina)) { |
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236 | char *description = GBS_global_string_copy("the sina executable derived from\n" |
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237 | "- gui property '%s' and\n" |
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238 | "- environment variable PATH", sinaName); |
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239 | ARB_warn_about_unwanted_chars(sina, description); |
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240 | free(description); |
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241 | } |
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242 | |
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243 | for (int v = 0; ; ++v) { |
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244 | const sina_arb_interface_def& sina_interface = supported_sina_versions[v]; |
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245 | |
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246 | if (sina_interface.version == NO_INTERFACE) { |
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247 | if (fail_reason) fail_reason = GBS_global_string("%s (this ARB supports these interface versions: %s)", fail_reason, tested_interfaces.c_str()); |
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248 | break; |
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249 | } |
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250 | |
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251 | int exitstatus = system(GBS_global_string("%s --has-cli-vers %s", sina, sina_interface.id)); |
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252 | exitstatus = WEXITSTATUS(exitstatus); |
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253 | |
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254 | if (exitstatus == EXIT_FAILURE) { |
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255 | fail_reason = "Incompatible SINA and ARB versions"; |
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256 | |
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257 | if (!tested_interfaces.empty()) tested_interfaces += ' '; |
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258 | tested_interfaces += sina_interface.id; |
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259 | } |
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260 | else { |
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261 | if (exitstatus == EXIT_SUCCESS) { |
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262 | used_sina_interface = sina_interface.version; |
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263 | fail_reason = NULp; |
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264 | |
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265 | fprintf(stdout, "Using SINA-ARB interface version '%s'%s\n", sina_interface.id, v>0 ? " (old)" : ""); |
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266 | } |
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267 | else { |
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268 | fail_reason = GBS_global_string("Could not execute SINA binary '%s' (exitstatus was %i)", |
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269 | sinaName, exitstatus); |
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270 | } |
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271 | break; // either found compatible version or failed completely -> stop |
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272 | } |
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273 | } |
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274 | |
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275 | free(sina); |
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276 | } |
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277 | else { |
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278 | fail_reason = GBS_global_string("'%s' not found", sinaName); |
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279 | } |
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280 | |
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281 | return fail_reason; |
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282 | } |
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283 | |
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284 | AW_active sina_mask(AW_root *awr) { |
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285 | GB_ERROR fail_reason = detect_sina_version(awr); |
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286 | |
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287 | if (fail_reason) { |
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288 | fprintf(stderr, "Note: SINA (SILVA Aligner) disabled (Reason: %s)\n", fail_reason); |
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289 | } |
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290 | |
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291 | return fail_reason ? AWM_DISABLED : AWM_ALL; |
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292 | } |
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293 | |
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294 | inline const char *stream2static(const std::stringstream& str) { |
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295 | return GBS_static_string(str.str().c_str()); |
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296 | } |
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297 | |
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298 | inline const char *empty_as_none(const char *sainame) { |
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299 | // see http://bugs.arb-home.de/ticket/519 |
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300 | if (sainame && !sainame[0]) sainame = "none"; |
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301 | return sainame; |
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302 | } |
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303 | |
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304 | static void sina_start(AW_window *window, const AlignDataAccess *data_access) { |
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305 | cerr << "Starting SINA..." << endl; |
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306 | |
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307 | AW_root *root = window->get_root(); |
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308 | GB_ERROR gb_error = NULp; |
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309 | |
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310 | // make string from pt server selection |
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311 | const char *pt_server = NULp; |
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312 | { |
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313 | int pt = root->awar(AWAR_PT_SERVER)->read_int(); |
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314 | if (pt == -1) { // do NOT use ptserver (aka "use sina internal family search") |
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315 | if (used_sina_interface == ARB_5_99) { |
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316 | gb_error = "internal SINA family search is not available with your SINA version"; |
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317 | } |
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318 | } |
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319 | else { |
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320 | std::stringstream ptnam; |
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321 | ptnam << "ARB_PT_SERVER" << pt; |
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322 | |
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323 | pt_server = GBS_read_arb_tcp(ptnam.str().c_str()); |
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324 | if (!pt_server) { |
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325 | gb_error = GBS_global_string("Unable to find definition for chosen PT-server\n(Reason: %s)", GB_await_error()); |
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326 | } |
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327 | else { |
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328 | const char *pt_db = pt_server + strlen(pt_server) + 1; |
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329 | pt_db += strlen(pt_db)+3; |
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330 | |
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331 | // start pt server if necessary |
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332 | gb_error = arb_look_and_start_server(AISC_MAGIC_NUMBER, ptnam.str().c_str()); |
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333 | if (gb_error) { |
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334 | std::stringstream tmp; |
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335 | tmp << "Cannot contact PT server. Aborting" << endl |
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336 | << " ID: \"" << tmp.str().c_str() |
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337 | << "\" PORT: \"" << pt_server |
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338 | << "\" DB: \"" << pt_db << endl |
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339 | << "\" GBERROR: \"" << gb_error << "\"" << endl; |
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340 | gb_error = stream2static(tmp); |
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341 | } |
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342 | } |
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343 | } |
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344 | } |
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345 | |
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346 | if (!gb_error) { |
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347 | // create temporary file |
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348 | char* tmpfile; |
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349 | FILE* tmpfile_F; |
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350 | { |
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351 | char* tmpfile_tpl = GB_unique_filename("sina_select", "tmp"); |
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352 | tmpfile_F = GB_fopen_tempfile(tmpfile_tpl, "w", &tmpfile); |
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353 | free(tmpfile_tpl); |
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354 | } |
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355 | |
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356 | if (!tmpfile_F) { |
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357 | std::stringstream tmp; |
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358 | tmp << "Error: Unable to create temporary file \"" << tmpfile << "\"!"; |
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359 | gb_error = stream2static(tmp); |
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360 | } |
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361 | else { |
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362 | GB_remove_on_exit(tmpfile); |
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363 | GBDATA *gb_main = data_access->gb_main; |
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364 | |
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365 | std::vector<std::string> spec_names; |
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366 | switch (root->awar(GA_AWAR_TGT)->read_int()) { |
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367 | case 0: { // current |
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368 | char *spec_name = root->awar(AWAR_SPECIES_NAME)->read_string(); |
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369 | if (spec_name) { |
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370 | fwrite(spec_name, strlen(spec_name), 1, tmpfile_F); |
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371 | fwrite("\n", 1, 1, tmpfile_F); |
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372 | } |
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373 | else { |
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374 | gb_error = "Unable to get name of currently active species"; |
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375 | } |
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376 | free(spec_name); |
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377 | break; |
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378 | } |
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379 | case 1: { // selected |
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380 | GB_begin_transaction(gb_main); |
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381 | int num_selected = 0; |
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382 | for (GBDATA *gb_spec = data_access->get_first_selected_species(&num_selected); |
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383 | gb_spec; |
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384 | gb_spec = data_access->get_next_selected_species()) |
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385 | { |
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386 | GBDATA *gbd_name = GB_find(gb_spec, "name", SEARCH_CHILD); |
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387 | if (gbd_name) { |
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388 | const char *str = GB_read_char_pntr(gbd_name); |
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389 | fwrite(str, strlen(str), 1, tmpfile_F); |
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390 | fwrite("\n", 1, 1, tmpfile_F); |
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391 | } |
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392 | } |
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393 | GB_commit_transaction(gb_main); |
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394 | break; |
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395 | } |
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396 | case 2: { // marked |
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397 | GB_begin_transaction(gb_main); |
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398 | for (GBDATA *gb_spec = GBT_first_marked_species(gb_main); |
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399 | gb_spec; gb_spec = GBT_next_marked_species(gb_spec)) { |
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400 | GBDATA *gbd_name = GB_find(gb_spec, "name", SEARCH_CHILD); |
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401 | if (gbd_name) { |
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402 | const char *str = GB_read_char_pntr(gbd_name); |
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403 | fwrite(str, strlen(str), 1, tmpfile_F); |
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404 | fwrite("\n", 1, 1, tmpfile_F); |
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405 | } |
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406 | } |
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407 | GB_commit_transaction(gb_main); |
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408 | break; |
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409 | } |
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410 | } |
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411 | fclose(tmpfile_F); |
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412 | |
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413 | if (!gb_error) { |
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414 | // build command line |
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415 | GBS_strstruct cl(2000); |
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416 | |
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417 | cl.cat(root->awar(GA_AWAR_CMD)->read_char_pntr()); |
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418 | cl.cat(" -i :"); |
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419 | |
---|
420 | switch (used_sina_interface) { |
---|
421 | case ARB_5_99: cl.cat(" --ptdb :"); break; |
---|
422 | case ARB_7_1: |
---|
423 | cl.cat(" --out :"); |
---|
424 | cl.cat(" --db "); cl.cat(root->awar(GA_AWAR_REFDB)->read_char_pntr()); |
---|
425 | if (root->awar(GA_AWAR_NOWARN_START)->read_int()) cl.cat(" --dont-expect-start"); |
---|
426 | if (root->awar(GA_AWAR_CALC_IDENTITY)->read_int()) cl.cat(" --calc-idty"); |
---|
427 | cl.cat(" --default-alignment "); cl.cat(data_access->alignment_name.c_str()); // always use alignment used in editor |
---|
428 | break; |
---|
429 | } |
---|
430 | |
---|
431 | if (pt_server) { |
---|
432 | cl.cat(" --ptport "); cl.cat(pt_server); |
---|
433 | if (used_sina_interface == ARB_7_1) { |
---|
434 | cl.cat(" --fs-engine "); cl.cat("pt-server"); |
---|
435 | } |
---|
436 | } |
---|
437 | else { // use sina internal search |
---|
438 | ga_assert(used_sina_interface == ARB_7_1); |
---|
439 | cl.cat(" --fs-engine "); cl.cat("internal"); |
---|
440 | } |
---|
441 | |
---|
442 | cl.cat(" --turn "); cl.cat(root->awar(GA_AWAR_TURN_CHECK)->read_int() ? "all" : "none"); |
---|
443 | cl.cat(" --overhang "); cl.cat(root->awar(GA_AWAR_OVERHANG)->read_char_pntr()); |
---|
444 | cl.cat(" --filter "); cl.cat(empty_as_none(root->awar(GA_AWAR_SAI)->read_char_pntr())); |
---|
445 | cl.cat(" --fs-min "); cl.putlong(root->awar(GA_AWAR_FS_MIN)->read_int()); |
---|
446 | cl.cat(" --fs-msc "); cl.putfloat(root->awar(GA_AWAR_FS_MSC)->read_float()); |
---|
447 | cl.cat(" --fs-max "); cl.putlong(root->awar(GA_AWAR_FS_MAX)->read_int()); |
---|
448 | cl.cat(" --fs-req 1"); // hardcoded to 1 - uses stricter requirement for full-length sequences |
---|
449 | cl.cat(" --fs-req-full "); cl.putlong(root->awar(GA_AWAR_MIN_FULL)->read_int()); |
---|
450 | cl.cat(" --fs-full-len "); cl.putlong(root->awar(GA_AWAR_FULL_MINLEN)->read_int()); |
---|
451 | cl.cat(" --fs-kmer-len "); cl.putlong(root->awar(GA_AWAR_KMER_LEN)->read_int()); |
---|
452 | cl.cat(" --fs-kmer-mm "); cl.putlong(root->awar(GA_AWAR_KMER_MM)->read_int()); // @@@ only with ptserver -> document + add 2 gui |
---|
453 | cl.cat(" --fs-min-len "); cl.putlong(root->awar(GA_AWAR_MIN_LEN)->read_int()); |
---|
454 | cl.cat(" --fs-weight "); cl.putfloat(root->awar(GA_AWAR_WEIGHT)->read_float()); |
---|
455 | cl.cat(" --pen-gap "); cl.putfloat(root->awar(GA_AWAR_GAP_PEN)->read_float()); |
---|
456 | cl.cat(" --pen-gapext "); cl.putfloat(root->awar(GA_AWAR_GAP_EXT)->read_float()); |
---|
457 | cl.cat(" --match-score "); cl.putfloat(root->awar(GA_AWAR_MATCH_SCORE)->read_float()); |
---|
458 | cl.cat(" --mismatch-score "); cl.putfloat(root->awar(GA_AWAR_MISMATCH_SCORE)->read_float()); |
---|
459 | cl.cat(" --prot-level "); cl.putlong(root->awar(GA_AWAR_PROTECTION)->read_int()); |
---|
460 | cl.cat(" --select-file "); cl.cat(tmpfile); |
---|
461 | cl.cat(" --insertion "); cl.cat(root->awar(GA_AWAR_INSERT)->read_char_pntr()); |
---|
462 | cl.cat(" --lowercase "); cl.cat(root->awar(GA_AWAR_LOWERCASE)->read_char_pntr()); |
---|
463 | cl.cat(" --auto-filter-field "); cl.cat_sQuoted(root->awar(GA_AWAR_AUTOFILTER)->read_char_pntr()); // note: default value passed to sina 1.3 was 'none' (w/o quotes) |
---|
464 | cl.cat(" --gene-start "); cl.putlong(root->awar(GA_AWAR_GENE_START)->read_int()); |
---|
465 | cl.cat(" --gene-end "); cl.putlong(root->awar(GA_AWAR_GENE_END)->read_int()); |
---|
466 | cl.cat(" --fs-cover-gene "); cl.putlong(root->awar(GA_AWAR_FS_COVER_GENE)->read_int()); |
---|
467 | |
---|
468 | |
---|
469 | if (root->awar(GA_AWAR_KMER_NOREL)->read_int()) cl.cat(" --fs-kmer-norel "); |
---|
470 | if (root->awar(GA_AWAR_KMER_NOFAST)->read_int()) cl.cat(" --fs-kmer-no-fast "); |
---|
471 | if (root->awar(GA_AWAR_SHOW_DIST)->read_int()) cl.cat(" --show-dist "); |
---|
472 | if (root->awar(GA_AWAR_SHOW_DIFF)->read_int()) cl.cat(" --show-diff "); |
---|
473 | if (root->awar(GA_AWAR_COLOR)->read_int()) cl.cat(" --color"); |
---|
474 | if (root->awar(GA_AWAR_REALIGN)->read_int()) cl.cat(" --realign"); |
---|
475 | if (root->awar(GA_AWAR_USED_RELS)->read_int()) cl.cat(" --write-used-rels"); |
---|
476 | |
---|
477 | gb_error = GB_xcmd(cl.get_data(), XCMD_ASYNC_WAITKEY); |
---|
478 | } |
---|
479 | |
---|
480 | if (!gb_error) aw_message("SINA finished aligning."); |
---|
481 | } |
---|
482 | free(tmpfile); |
---|
483 | } |
---|
484 | |
---|
485 | aw_message_if(gb_error); |
---|
486 | } |
---|
487 | |
---|
488 | |
---|
489 | |
---|
490 | static char* filter_posvar_SAI_for_ali(GBDATA *gb_extended, const char *ali_name) { |
---|
491 | char *result = NULp; |
---|
492 | char *typePath = GBS_global_string_copy("%s/_TYPE", ali_name); |
---|
493 | GBDATA *gbd = GB_search(gb_extended, typePath, GB_FIND); |
---|
494 | if (gbd) { |
---|
495 | const char* type = GB_read_char_pntr(gbd); |
---|
496 | if (type && strncmp("PV", type, 2) == 0) { |
---|
497 | gbd = GB_find(gb_extended, "name", SEARCH_CHILD); |
---|
498 | result = GB_read_string(gbd); |
---|
499 | } |
---|
500 | } |
---|
501 | free(typePath); |
---|
502 | return result; |
---|
503 | } |
---|
504 | |
---|
505 | static AWT_config_mapping_def sina_config_mapping[] = { |
---|
506 | // both |
---|
507 | { GA_AWAR_TGT, "target" }, |
---|
508 | { GA_AWAR_OVERHANG, "overhang" }, |
---|
509 | { GA_AWAR_INSERT, "insertions" }, |
---|
510 | { GA_AWAR_LOWERCASE, "lowercase" }, |
---|
511 | { GA_AWAR_WEIGHT, "weight" }, |
---|
512 | { GA_AWAR_FULL_MINLEN, "fullminlen" }, |
---|
513 | |
---|
514 | // advanced only |
---|
515 | { GA_AWAR_SAI, "sai" }, |
---|
516 | { GA_AWAR_AUTOFILTER, "autofilter" }, |
---|
517 | { GA_AWAR_TURN_CHECK, "turncheck" }, |
---|
518 | { GA_AWAR_REALIGN, "realign" }, |
---|
519 | { GA_AWAR_GAP_PEN, "gappen" }, |
---|
520 | { GA_AWAR_GAP_EXT, "gapext" }, |
---|
521 | { GA_AWAR_MATCH_SCORE, "matchscore" }, |
---|
522 | { GA_AWAR_MISMATCH_SCORE, "mismatchscore" }, |
---|
523 | { GA_AWAR_FS_MIN, "fs_min" }, |
---|
524 | { GA_AWAR_FS_MSC, "fs_minscore" }, |
---|
525 | { GA_AWAR_FS_MAX, "fs_min" }, |
---|
526 | { GA_AWAR_MIN_FULL, "minfull" }, |
---|
527 | { GA_AWAR_KMER_LEN, "kmerlen" }, |
---|
528 | { GA_AWAR_KMER_MM, "kmermm" }, |
---|
529 | { GA_AWAR_MIN_LEN, "refminlen" }, |
---|
530 | { GA_AWAR_GENE_START, "genestart" }, |
---|
531 | { GA_AWAR_GENE_END, "geneend" }, |
---|
532 | { GA_AWAR_FS_COVER_GENE, "fs_covergene" }, |
---|
533 | { GA_AWAR_USED_RELS, "write_used_rels" }, |
---|
534 | { GA_AWAR_CALC_IDENTITY, "calc_identity" }, |
---|
535 | { GA_AWAR_KMER_NOFAST, "kmernofast" }, |
---|
536 | { GA_AWAR_KMER_NOREL, "kmernorel" }, |
---|
537 | |
---|
538 | // both |
---|
539 | { GA_AWAR_SHOW_DIFF, "showdiff" }, |
---|
540 | { GA_AWAR_COLOR, "color" }, |
---|
541 | { GA_AWAR_SHOW_DIST, "showdist" }, |
---|
542 | |
---|
543 | { NULp, NULp } |
---|
544 | }; |
---|
545 | |
---|
546 | static void accept_selected_ref_db(AW_window *aww, const char * const *awar_filename) { |
---|
547 | AW_root *root = aww->get_root(); |
---|
548 | char *filename = root->awar(*awar_filename)->read_string(); |
---|
549 | |
---|
550 | if (GB_is_readablefile(filename)) { |
---|
551 | AW_awar *awar_refdb_sel = root->awar(GA_AWAR_REFDB_SELECT); |
---|
552 | awar_refdb_sel->write_string(filename); |
---|
553 | aww->hide(); |
---|
554 | } |
---|
555 | |
---|
556 | free(filename); |
---|
557 | } |
---|
558 | |
---|
559 | static AW_window *create_ref_db_selection_window(AW_root *aw_root) { |
---|
560 | static char *awar_name = NULp; // do not free, bound to callback |
---|
561 | return awt_create_load_box(aw_root, |
---|
562 | "Select", |
---|
563 | "SINA reference database", |
---|
564 | ".", "arb", &awar_name, |
---|
565 | makeWindowCallback(accept_selected_ref_db, (const char*const*)&awar_name)); |
---|
566 | } |
---|
567 | |
---|
568 | |
---|
569 | static void sina_select_ref_db(AW_window *aww) { |
---|
570 | static AW_window *aw_ref_db_selection = NULL; |
---|
571 | |
---|
572 | if (!aw_ref_db_selection) { |
---|
573 | AW_root *root = aww->get_root(); |
---|
574 | aw_ref_db_selection = create_ref_db_selection_window(root); |
---|
575 | } |
---|
576 | |
---|
577 | aw_ref_db_selection->activate(); |
---|
578 | } |
---|
579 | |
---|
580 | |
---|
581 | static SinaArbInterfaceVersion gui_sina_interface = NO_INTERFACE; // interface used in GUI |
---|
582 | |
---|
583 | static void sina_cmd_changed_cb(AW_root *awr) { |
---|
584 | GB_ERROR fail_reason = detect_sina_version(awr); |
---|
585 | |
---|
586 | if (fail_reason) { |
---|
587 | aw_message(fail_reason); |
---|
588 | } |
---|
589 | else { |
---|
590 | if (used_sina_interface != gui_sina_interface) { |
---|
591 | aw_message("The GUI does not match the used sina version!\n" |
---|
592 | "Please save properties and restart the editor."); |
---|
593 | } |
---|
594 | aw_message(GBS_global_string("Using sina-arb interface '%s'", get_interface_id(used_sina_interface))); |
---|
595 | } |
---|
596 | } |
---|
597 | |
---|
598 | static AW_window_simple* new_sina_simple(AW_root *root, const AlignDataAccess *alignData, bool adv) { |
---|
599 | const int hgap = 10; |
---|
600 | AW_window_simple *aws = new AW_window_simple; |
---|
601 | |
---|
602 | if (gui_sina_interface == NO_INTERFACE) { |
---|
603 | gui_sina_interface = used_sina_interface; |
---|
604 | root->awar(GA_AWAR_CMD)->add_callback(sina_cmd_changed_cb); |
---|
605 | } |
---|
606 | |
---|
607 | aws->reset_layout("20220422"); |
---|
608 | aws->init(root, adv ? "XSINA" : "SINA", "SINA (SILVA Incremental Aligner)"); |
---|
609 | |
---|
610 | aws->button_length(12); |
---|
611 | aws->at(10, 10); |
---|
612 | aws->auto_space(5, 5); |
---|
613 | |
---|
614 | int closex, closey; aws->get_at_position(&closex, &closey); |
---|
615 | |
---|
616 | aws->callback(AW_POPDOWN); |
---|
617 | aws->create_button("CLOSE", "CLOSE", "O"); |
---|
618 | |
---|
619 | int closex2 = aws->get_at_xposition(); |
---|
620 | |
---|
621 | if (gui_sina_interface == NO_INTERFACE) { |
---|
622 | // happens when no sina detected + menu entry is triggered via macro // @@@ would be nice to globally avoid callbacks for inactive menu entries |
---|
623 | |
---|
624 | aws->at_newline(); |
---|
625 | aws->label_length(0); |
---|
626 | aws->label("SINA command\nError: Failed to find or identify sina version!"); |
---|
627 | aws->create_input_field(GA_AWAR_CMD, 20); |
---|
628 | |
---|
629 | return aws; |
---|
630 | } |
---|
631 | |
---|
632 | aws->at_shift(10, 0); |
---|
633 | aws->label_length(0); |
---|
634 | aws->label("Show advanced options"); |
---|
635 | aws->create_toggle(GA_AWAR_ADVANCED); |
---|
636 | |
---|
637 | bool cbInstalled = false; |
---|
638 | if (!cbInstalled) { |
---|
639 | root->awar(GA_AWAR_ADVANCED)->add_callback(makeRootCallback(show_sina_window, alignData)); |
---|
640 | cbInstalled = true; |
---|
641 | } |
---|
642 | |
---|
643 | aws->at_newline(); |
---|
644 | aws->at_shift(0, hgap); |
---|
645 | int y_col2 = aws->get_at_yposition(); |
---|
646 | aws->label_length(15); |
---|
647 | aws->create_toggle_field(GA_AWAR_TGT, "Align what?"); |
---|
648 | aws->insert_toggle("Current Species:", "C", 0); |
---|
649 | aws->insert_toggle("Selected Species", "S", 1); |
---|
650 | aws->insert_default_toggle("Marked Species", "M", 2); |
---|
651 | aws->update_toggle_field(); |
---|
652 | |
---|
653 | aws->at_shift(0, 3); |
---|
654 | aws->create_input_field(AWAR_SPECIES_NAME, 20); |
---|
655 | |
---|
656 | aws->at_newline(); |
---|
657 | aws->at_shift(0, hgap); |
---|
658 | aws->button_length(24); |
---|
659 | if (used_sina_interface == ARB_5_99) { |
---|
660 | aws->label("PT Server:"); |
---|
661 | } |
---|
662 | else { |
---|
663 | aws->label_length(20); |
---|
664 | aws->label("PT Server\n" |
---|
665 | "undefined => sina kmer"); |
---|
666 | } |
---|
667 | awt_create_PTSERVER_selection_button(aws, AWAR_PT_SERVER); |
---|
668 | |
---|
669 | aws->label_length(0); |
---|
670 | |
---|
671 | if (gui_sina_interface == ARB_7_1) { |
---|
672 | aws->at_newline(); |
---|
673 | aws->create_toggle_field(GA_AWAR_REFDB_MODE, "Reference database"); |
---|
674 | aws->insert_toggle("Explicit as ", "E", REF_EXPLICIT); |
---|
675 | aws->insert_toggle("Last saved", "s", REF_LAST_SAVE); |
---|
676 | aws->insert_default_toggle("Current", "C", REF_CURRENT); |
---|
677 | aws->update_toggle_field(); |
---|
678 | |
---|
679 | aws->at_shift(0, 3); |
---|
680 | aws->callback(makeWindowCallback(sina_select_ref_db)); |
---|
681 | aws->create_autosize_button("selected", "selected", "s"); |
---|
682 | |
---|
683 | aws->at_newline(); |
---|
684 | aws->create_input_field(GA_AWAR_REFDB, 40); |
---|
685 | } |
---|
686 | |
---|
687 | aws->at_newline(); |
---|
688 | aws->label("Overhang placement"); |
---|
689 | aws->create_option_menu(GA_AWAR_OVERHANG); |
---|
690 | aws->insert_option("keep attached", NULp, "attach"); |
---|
691 | aws->insert_option("move to edge", NULp, "edge"); |
---|
692 | aws->insert_option("remove", NULp, "remove"); |
---|
693 | aws->update_option_menu(); |
---|
694 | |
---|
695 | aws->at_newline(); |
---|
696 | aws->label("Handling of unmappable insertions"); |
---|
697 | aws->create_option_menu(GA_AWAR_INSERT); |
---|
698 | aws->insert_option("Shift surrounding bases", NULp, "shift"); |
---|
699 | aws->insert_option("Forbid during DP alignment", NULp, "forbid"); |
---|
700 | aws->insert_option("Delete bases", NULp, "remove"); |
---|
701 | aws->update_option_menu(); |
---|
702 | |
---|
703 | int x_col2 = aws->get_at_xposition(); |
---|
704 | |
---|
705 | aws->at_newline(); |
---|
706 | aws->label("Character Case"); |
---|
707 | aws->create_option_menu(GA_AWAR_LOWERCASE); |
---|
708 | aws->insert_option("Do not modify", NULp, "original"); |
---|
709 | aws->insert_option("Show unaligned bases as lower case", NULp, "unaligned"); |
---|
710 | aws->insert_option("Uppercase all", NULp, "none"); |
---|
711 | aws->update_option_menu(); |
---|
712 | |
---|
713 | aws->at_newline(); |
---|
714 | aws->label("Family conservation weight (0-1)"); |
---|
715 | aws->create_input_field(GA_AWAR_WEIGHT, 3); |
---|
716 | |
---|
717 | aws->at_newline(); |
---|
718 | aws->label("Size of full-length sequences"); |
---|
719 | aws->create_input_field(GA_AWAR_FULL_MINLEN, 5); |
---|
720 | |
---|
721 | aws->at_newline(); |
---|
722 | aws->label_length(17); |
---|
723 | aws->label("Protection Level"); |
---|
724 | aws->create_option_menu(GA_AWAR_PROTECTION); |
---|
725 | aws->insert_option("0", NULp, 0); |
---|
726 | aws->insert_option("1", NULp, 1); |
---|
727 | aws->insert_option("2", NULp, 2); |
---|
728 | aws->insert_option("3", NULp, 3); |
---|
729 | aws->insert_option("4", NULp, 4); |
---|
730 | aws->insert_option("5", NULp, 5); |
---|
731 | aws->insert_option("6", NULp, 6); |
---|
732 | aws->update_option_menu(); |
---|
733 | |
---|
734 | aws->at_newline(); |
---|
735 | |
---|
736 | aws->label_length(21); |
---|
737 | aws->label("Show changed sections"); |
---|
738 | aws->create_toggle(GA_AWAR_SHOW_DIFF); |
---|
739 | aws->label_length(11); |
---|
740 | aws->label("color bases"); |
---|
741 | aws->create_toggle(GA_AWAR_COLOR); |
---|
742 | |
---|
743 | aws->at_newline(); |
---|
744 | aws->label_length(21); |
---|
745 | aws->label("Show statistics"); |
---|
746 | aws->create_toggle(GA_AWAR_SHOW_DIST); |
---|
747 | |
---|
748 | aws->at_newline(); |
---|
749 | |
---|
750 | int startx, starty; aws->get_at_position(&startx, &starty); |
---|
751 | |
---|
752 | aws->button_length(12); |
---|
753 | aws->at(closex, starty); |
---|
754 | aws->callback(makeWindowCallback(sina_start, alignData)); |
---|
755 | aws->highlight(); |
---|
756 | aws->create_button("Start", "Start", "S"); |
---|
757 | |
---|
758 | aws->at(closex2+200, starty-10); |
---|
759 | AWT_insert_config_manager(aws, AW_ROOT_DEFAULT, "sina", sina_config_mapping); |
---|
760 | |
---|
761 | if (adv) { |
---|
762 | aws->at(x_col2, y_col2); |
---|
763 | aws->auto_space(5, 5); // resets position for at_newline |
---|
764 | |
---|
765 | aws->label_length(0); |
---|
766 | aws->label("Pos. Var.:"); |
---|
767 | awt_create_SAI_selection_button(alignData->gb_main, aws, GA_AWAR_SAI, makeSaiSelectionlistFilterCallback(filter_posvar_SAI_for_ali, alignData->alignment_name.c_str())); |
---|
768 | |
---|
769 | aws->at_newline(); |
---|
770 | aws->label("Field used for automatic\nfilter selection"); |
---|
771 | aws->create_input_field(GA_AWAR_AUTOFILTER, 20); |
---|
772 | |
---|
773 | aws->at_newline(); |
---|
774 | aws->label("Turn check"); aws->create_toggle(GA_AWAR_TURN_CHECK); |
---|
775 | aws->label("Realign"); aws->create_toggle(GA_AWAR_REALIGN); |
---|
776 | |
---|
777 | aws->at_newline(); |
---|
778 | aws->at_shift(0, hgap); |
---|
779 | |
---|
780 | aws->label("Gap insertion/extension penalties"); |
---|
781 | aws->create_input_field(GA_AWAR_GAP_PEN, 5); |
---|
782 | aws->create_input_field(GA_AWAR_GAP_EXT, 5); |
---|
783 | |
---|
784 | aws->at_newline(); |
---|
785 | aws->label("Match score"); |
---|
786 | aws->create_input_field(GA_AWAR_MATCH_SCORE, 3); |
---|
787 | aws->label("Mismatch score"); |
---|
788 | aws->create_input_field(GA_AWAR_MISMATCH_SCORE, 3); |
---|
789 | |
---|
790 | aws->at_newline(); |
---|
791 | aws->label("Family search min/min_score/max"); |
---|
792 | aws->create_input_field(GA_AWAR_FS_MIN, 3); |
---|
793 | aws->create_input_field(GA_AWAR_FS_MSC, 3); |
---|
794 | aws->create_input_field(GA_AWAR_FS_MAX, 3); |
---|
795 | |
---|
796 | aws->at_newline(); |
---|
797 | aws->label("Minimal number of full length sequences"); |
---|
798 | aws->create_input_field(GA_AWAR_MIN_FULL, 3); |
---|
799 | |
---|
800 | aws->at_newline(); |
---|
801 | aws->label("Family search oligo length / mismatches"); |
---|
802 | aws->create_input_field(GA_AWAR_KMER_LEN, 3); |
---|
803 | aws->create_input_field(GA_AWAR_KMER_MM, 3); |
---|
804 | |
---|
805 | aws->at_newline(); |
---|
806 | aws->label("Minimal reference sequence length"); |
---|
807 | aws->create_input_field(GA_AWAR_MIN_LEN, 5); |
---|
808 | |
---|
809 | aws->at_newline(); |
---|
810 | aws->label("Alignment bounds: start"); |
---|
811 | aws->create_input_field(GA_AWAR_GENE_START, 6); |
---|
812 | aws->label("end"); |
---|
813 | aws->create_input_field(GA_AWAR_GENE_END, 6); |
---|
814 | |
---|
815 | aws->at_newline(); |
---|
816 | aws->label("Number of references required to touch bounds"); |
---|
817 | aws->create_input_field(GA_AWAR_FS_COVER_GENE, 3); |
---|
818 | |
---|
819 | aws->at_newline(); |
---|
820 | aws->label("Save used references in 'used_rels'"); |
---|
821 | aws->create_toggle(GA_AWAR_USED_RELS); |
---|
822 | |
---|
823 | aws->at_newline(); |
---|
824 | aws->label("Store highest identity in 'align_ident_slv'"); |
---|
825 | aws->create_toggle(GA_AWAR_CALC_IDENTITY); |
---|
826 | |
---|
827 | aws->at_newline(); |
---|
828 | aws->label("Disable fast search"); |
---|
829 | aws->create_toggle(GA_AWAR_KMER_NOFAST); |
---|
830 | |
---|
831 | aws->at_newline(); |
---|
832 | aws->label("Score search results by absolute oligo match count"); |
---|
833 | aws->create_toggle(GA_AWAR_KMER_NOREL); |
---|
834 | |
---|
835 | if (gui_sina_interface == ARB_7_1) { |
---|
836 | aws->at_newline(); |
---|
837 | aws->label("Suppress warnings about missing 'start' field"); |
---|
838 | aws->create_toggle(GA_AWAR_NOWARN_START); |
---|
839 | } |
---|
840 | |
---|
841 | aws->at_newline(); |
---|
842 | aws->label("SINA command"); |
---|
843 | aws->create_input_field(GA_AWAR_CMD, 20); |
---|
844 | } |
---|
845 | |
---|
846 | int winx, winy; aws->get_window_size(winx, winy); |
---|
847 | |
---|
848 | aws->button_length(12); |
---|
849 | aws->at(winx-closex2+5, closey); |
---|
850 | aws->callback(makeHelpCallback("sina_main.hlp")); |
---|
851 | aws->create_button("HELP", "HELP"); |
---|
852 | |
---|
853 | return aws; |
---|
854 | } |
---|
855 | |
---|
856 | void show_sina_window(UNFIXED, const AlignDataAccess *alignData) { |
---|
857 | AW_root *root = AW_root::SINGLETON; |
---|
858 | |
---|
859 | static AW_window_simple *ga_aws = NULp; |
---|
860 | static AW_window_simple *ga_aws_adv = NULp; |
---|
861 | |
---|
862 | if (root->awar(GA_AWAR_ADVANCED)->read_int()) { |
---|
863 | if (!ga_aws_adv) ga_aws_adv = new_sina_simple(root, alignData, true); |
---|
864 | ga_aws_adv->show(); |
---|
865 | |
---|
866 | if (ga_aws) ga_aws->hide(); |
---|
867 | } |
---|
868 | else { |
---|
869 | if (!ga_aws) ga_aws = new_sina_simple(root, alignData, false); |
---|
870 | ga_aws->show(); |
---|
871 | |
---|
872 | if (ga_aws_adv) ga_aws_adv->hide(); |
---|
873 | } |
---|
874 | |
---|
875 | static bool refdb_mode_refreshed = false; |
---|
876 | if (!refdb_mode_refreshed) { |
---|
877 | AW_awar *awar_mode = root->awar(GA_AWAR_REFDB_MODE); |
---|
878 | if (awar_mode->read_int() != REF_EXPLICIT) awar_mode->touch(); // trigger callback |
---|
879 | refdb_mode_refreshed = true; |
---|
880 | } |
---|
881 | } |
---|
882 | |
---|