1 | #include "muscle.h" |
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2 | #include "objscore.h" |
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3 | #include "profile.h" |
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4 | #include "enumopts.h" |
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5 | |
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6 | const double DEFAULT_MAX_MB_FRACT = 0.8; |
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7 | |
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8 | SCORE g_scoreCenter = 0; |
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9 | SCORE g_scoreGapExtend = 0; |
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10 | SCORE g_scoreGapOpen2 = MINUS_INFINITY; |
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11 | SCORE g_scoreGapExtend2 = MINUS_INFINITY; |
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12 | SCORE g_scoreGapAmbig = 0; |
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13 | SCORE g_scoreAmbigFactor = 0; |
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14 | |
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15 | extern SCOREMATRIX VTML_LA; |
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16 | extern SCOREMATRIX PAM200; |
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17 | extern SCOREMATRIX PAM200NoCenter; |
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18 | extern SCOREMATRIX VTML_SP; |
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19 | extern SCOREMATRIX VTML_SPNoCenter; |
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20 | extern SCOREMATRIX NUC_SP; |
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21 | |
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22 | PTR_SCOREMATRIX g_ptrScoreMatrix; |
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23 | |
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24 | const char *g_pstrInFileName = "-"; |
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25 | const char *g_pstrOutFileName = "-"; |
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26 | const char *g_pstrFASTAOutFileName = 0; |
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27 | const char *g_pstrMSFOutFileName = 0; |
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28 | const char *g_pstrClwOutFileName = 0; |
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29 | const char *g_pstrClwStrictOutFileName = 0; |
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30 | const char *g_pstrHTMLOutFileName = 0; |
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31 | const char *g_pstrPHYIOutFileName = 0; |
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32 | const char *g_pstrPHYSOutFileName = 0; |
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33 | const char *g_pstrDistMxFileName1 = 0; |
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34 | const char *g_pstrDistMxFileName2 = 0; |
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35 | |
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36 | const char *g_pstrFileName1 = 0; |
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37 | const char *g_pstrFileName2 = 0; |
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38 | |
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39 | const char *g_pstrSPFileName = 0; |
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40 | const char *g_pstrMatrixFileName = 0; |
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41 | |
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42 | const char *g_pstrUseTreeFileName = 0; |
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43 | bool g_bUseTreeNoWarn = false; |
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44 | |
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45 | const char *g_pstrComputeWeightsFileName; |
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46 | const char *g_pstrScoreFileName; |
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47 | |
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48 | const char *g_pstrProf1FileName = 0; |
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49 | const char *g_pstrProf2FileName = 0; |
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50 | |
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51 | unsigned g_uSmoothWindowLength = 7; |
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52 | unsigned g_uAnchorSpacing = 32; |
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53 | unsigned g_uMaxTreeRefineIters = 1; |
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54 | |
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55 | unsigned g_uRefineWindow = 200; |
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56 | unsigned g_uWindowFrom = 0; |
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57 | unsigned g_uWindowTo = 0; |
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58 | unsigned g_uSaveWindow = uInsane; |
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59 | unsigned g_uWindowOffset = 0; |
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60 | |
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61 | unsigned g_uMaxSubFamCount = 5; |
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62 | |
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63 | unsigned g_uHydrophobicRunLength = 5; |
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64 | float g_dHydroFactor = (float) 1.2; |
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65 | |
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66 | unsigned g_uMinDiagLength = 24; // TODO alpha -- should depend on alphabet? |
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67 | unsigned g_uMaxDiagBreak = 1; |
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68 | unsigned g_uDiagMargin = 5; |
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69 | |
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70 | float g_dSUEFF = (float) 0.1; |
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71 | |
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72 | bool g_bPrecompiledCenter = true; |
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73 | bool g_bNormalizeCounts = false; |
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74 | bool g_bDiags1 = false; |
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75 | bool g_bDiags2 = false; |
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76 | bool g_bAnchors = true; |
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77 | bool g_bQuiet = false; |
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78 | bool g_bVerbose = false; |
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79 | bool g_bRefine = false; |
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80 | bool g_bRefineW = false; |
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81 | bool g_bProfDB = false; |
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82 | bool g_bLow = false; |
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83 | bool g_bSW = false; |
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84 | bool g_bClusterOnly = false; |
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85 | bool g_bProfile = false; |
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86 | bool g_bPPScore = false; |
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87 | bool g_bBrenner = false; |
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88 | bool g_bDimer = false; |
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89 | bool g_bVersion = false; |
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90 | bool g_bStable = false; |
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91 | bool g_bFASTA = false; |
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92 | bool g_bPAS = false; |
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93 | bool g_bTomHydro = false; |
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94 | bool g_bMakeTree = false; |
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95 | |
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96 | #if DEBUG |
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97 | bool g_bCatchExceptions = false; |
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98 | #else |
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99 | bool g_bCatchExceptions = true; |
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100 | #endif |
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101 | |
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102 | bool g_bMSF = false; |
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103 | bool g_bAln = false; |
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104 | bool g_bClwStrict = false; |
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105 | bool g_bHTML = false; |
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106 | bool g_bPHYI = false; |
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107 | bool g_bPHYS = false; |
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108 | |
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109 | unsigned g_uMaxIters = 8; |
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110 | unsigned long g_ulMaxSecs = 0; |
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111 | unsigned g_uMaxMB = 500; |
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112 | |
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113 | PPSCORE g_PPScore = PPSCORE_LE; |
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114 | OBJSCORE g_ObjScore = OBJSCORE_SPM; |
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115 | |
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116 | SEQWEIGHT g_SeqWeight1 = SEQWEIGHT_ClustalW; |
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117 | SEQWEIGHT g_SeqWeight2 = SEQWEIGHT_ClustalW; |
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118 | |
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119 | DISTANCE g_Distance1 = DISTANCE_Kmer6_6; |
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120 | DISTANCE g_Distance2 = DISTANCE_PctIdKimura; |
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121 | |
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122 | CLUSTER g_Cluster1 = CLUSTER_UPGMB; |
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123 | CLUSTER g_Cluster2 = CLUSTER_UPGMB; |
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124 | |
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125 | ROOT g_Root1 = ROOT_Pseudo; |
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126 | ROOT g_Root2 = ROOT_Pseudo; |
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127 | |
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128 | bool g_bDiags; |
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129 | |
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130 | SEQTYPE g_SeqType = SEQTYPE_Auto; |
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131 | |
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132 | TERMGAPS g_TermGaps = TERMGAPS_Half; |
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133 | |
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134 | //------------------------------------------------------ |
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135 | // These parameters depending on the chosen prof-prof |
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136 | // score (g_PPScore), initialized to "Undefined". |
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137 | float g_dSmoothScoreCeil = fInsane; |
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138 | float g_dMinBestColScore = fInsane; |
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139 | float g_dMinSmoothScore = fInsane; |
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140 | SCORE g_scoreGapOpen = fInsane; |
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141 | //------------------------------------------------------ |
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142 | |
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143 | static unsigned atou(const char *s) |
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144 | { |
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145 | return (unsigned) atoi(s); |
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146 | } |
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147 | |
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148 | const char *MaxSecsToStr() |
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149 | { |
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150 | if (0 == g_ulMaxSecs) |
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151 | return "(No limit)"; |
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152 | return SecsToStr(g_ulMaxSecs); |
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153 | } |
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154 | |
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155 | void ListParams() |
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156 | { |
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157 | Log("\n"); |
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158 | Log("%s\n", MUSCLE_LONG_VERSION); |
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159 | Log("http://www.drive5.com/muscle\n"); |
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160 | Log("\n"); |
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161 | Log("Profile-profile score %s\n", PPSCOREToStr(g_PPScore)); |
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162 | Log("Max iterations %u\n", g_uMaxIters); |
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163 | Log("Max trees %u\n", g_uMaxTreeRefineIters); |
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164 | Log("Max time %s\n", MaxSecsToStr()); |
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165 | Log("Max MB %u\n", g_uMaxMB); |
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166 | Log("Gap open %g\n", g_scoreGapOpen); |
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167 | Log("Gap extend (dimer) %g\n", g_scoreGapExtend); |
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168 | Log("Gap ambig factor %g\n", g_scoreAmbigFactor); |
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169 | Log("Gap ambig penalty %g\n", g_scoreGapAmbig); |
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170 | Log("Center (LE) %g\n", g_scoreCenter); |
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171 | Log("Term gaps %s\n", TERMGAPSToStr(g_TermGaps)); |
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172 | |
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173 | Log("Smooth window length %u\n", g_uSmoothWindowLength); |
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174 | Log("Refine window length %u\n", g_uRefineWindow); |
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175 | Log("Min anchor spacing %u\n", g_uAnchorSpacing); |
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176 | Log("Min diag length (lambda) %u\n", g_uMinDiagLength); |
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177 | Log("Diag margin (mu) %u\n", g_uDiagMargin); |
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178 | Log("Min diag break %u\n", g_uMaxDiagBreak); |
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179 | Log("Hydrophobic window %u\n", g_uHydrophobicRunLength); |
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180 | |
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181 | Log("Hydrophobic gap factor %g\n", g_dHydroFactor); |
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182 | Log("Smooth score ceiling %g\n", g_dSmoothScoreCeil); |
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183 | Log("Min best col score %g\n", g_dMinBestColScore); |
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184 | Log("Min anchor score %g\n", g_dMinSmoothScore); |
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185 | Log("SUEFF %g\n", g_dSUEFF); |
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186 | |
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187 | Log("Brenner root MSA %s\n", BoolToStr(g_bBrenner)); |
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188 | Log("Normalize counts %s\n", BoolToStr(g_bNormalizeCounts)); |
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189 | Log("Diagonals (1) %s\n", BoolToStr(g_bDiags1)); |
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190 | Log("Diagonals (2) %s\n", BoolToStr(g_bDiags2)); |
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191 | Log("Anchors %s\n", BoolToStr(g_bAnchors)); |
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192 | Log("MSF output format %s\n", BoolToStr(g_bMSF)); |
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193 | Log("Phylip interleaved %s\n", BoolToStr(g_bPHYI)); |
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194 | Log("Phylip sequential %s\n", BoolToStr(g_bPHYS)); |
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195 | Log("ClustalW output format %s\n", BoolToStr(g_bAln)); |
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196 | Log("Catch exceptions %s\n", BoolToStr(g_bCatchExceptions)); |
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197 | Log("Quiet %s\n", BoolToStr(g_bQuiet)); |
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198 | Log("Refine %s\n", BoolToStr(g_bRefine)); |
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199 | Log("ProdfDB %s\n", BoolToStr(g_bProfDB)); |
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200 | Log("Low complexity profiles %s\n", BoolToStr(g_bLow)); |
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201 | |
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202 | Log("Objective score %s\n", OBJSCOREToStr(g_ObjScore)); |
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203 | |
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204 | Log("Distance method (1) %s\n", DISTANCEToStr(g_Distance1)); |
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205 | Log("Clustering method (1) %s\n", CLUSTERToStr(g_Cluster1)); |
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206 | Log("Root method (1) %s\n", ROOTToStr(g_Root1)); |
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207 | Log("Sequence weighting (1) %s\n", SEQWEIGHTToStr(g_SeqWeight1)); |
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208 | |
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209 | Log("Distance method (2) %s\n", DISTANCEToStr(g_Distance2)); |
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210 | Log("Clustering method (2) %s\n", CLUSTERToStr(g_Cluster2)); |
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211 | Log("Root method (2) %s\n", ROOTToStr(g_Root2)); |
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212 | Log("Sequence weighting (2) %s\n", SEQWEIGHTToStr(g_SeqWeight2)); |
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213 | |
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214 | Log("\n"); |
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215 | } |
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216 | |
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217 | static void SetDefaultsLE() |
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218 | { |
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219 | g_ptrScoreMatrix = &VTML_LA; |
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220 | |
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221 | //g_scoreGapOpen = (SCORE) -3.00; |
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222 | //g_scoreCenter = (SCORE) -0.55; |
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223 | g_scoreGapOpen = (SCORE) -2.9; |
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224 | g_scoreCenter = (SCORE) -0.52; |
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225 | |
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226 | g_bNormalizeCounts = true; |
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227 | |
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228 | //g_dSmoothScoreCeil = 5.0; |
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229 | //g_dMinBestColScore = 4.0; |
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230 | //g_dMinSmoothScore = 2.0; |
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231 | g_dSmoothScoreCeil = 3.0; |
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232 | g_dMinBestColScore = 2.0; |
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233 | g_dMinSmoothScore = 1.0; |
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234 | |
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235 | g_Distance1 = DISTANCE_Kmer6_6; |
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236 | g_Distance2 = DISTANCE_PctIdKimura; |
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237 | } |
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238 | |
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239 | static void SetDefaultsSP() |
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240 | { |
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241 | g_ptrScoreMatrix = &PAM200; |
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242 | |
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243 | g_scoreGapOpen = -1439; |
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244 | g_scoreCenter = 0.0; // center pre-added into score mx |
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245 | |
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246 | g_bNormalizeCounts = false; |
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247 | |
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248 | g_dSmoothScoreCeil = 200.0; |
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249 | g_dMinBestColScore = 300.0; |
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250 | g_dMinSmoothScore = 125.0; |
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251 | |
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252 | g_Distance1 = DISTANCE_Kmer6_6; |
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253 | g_Distance2 = DISTANCE_PctIdKimura; |
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254 | } |
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255 | |
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256 | static void SetDefaultsSV() |
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257 | { |
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258 | g_ptrScoreMatrix = &VTML_SP; |
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259 | |
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260 | g_scoreGapOpen = -300; |
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261 | g_scoreCenter = 0.0; // center pre-added into score mx |
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262 | |
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263 | g_bNormalizeCounts = false; |
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264 | |
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265 | g_dSmoothScoreCeil = 90.0; |
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266 | g_dMinBestColScore = 130.0; |
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267 | g_dMinSmoothScore = 40.0; |
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268 | |
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269 | g_Distance1 = DISTANCE_Kmer6_6; |
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270 | g_Distance2 = DISTANCE_PctIdKimura; |
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271 | } |
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272 | |
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273 | //static void SetDefaultsSPN() |
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274 | // { |
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275 | // g_ptrScoreMatrix = &NUC_SP; |
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276 | // |
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277 | // g_scoreGapOpen = -400; |
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278 | // g_scoreCenter = 0.0; // center pre-added into score mx |
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279 | // |
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280 | // g_bNormalizeCounts = false; |
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281 | // |
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282 | // g_dSmoothScoreCeil = 999.0; // disable |
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283 | // g_dMinBestColScore = 90; |
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284 | // g_dMinSmoothScore = 90; |
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285 | // |
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286 | // g_Distance1 = DISTANCE_Kmer4_6; |
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287 | // g_Distance2 = DISTANCE_PctIdKimura; |
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288 | // } |
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289 | |
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290 | static void SetDefaultsSPN_DNA() |
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291 | { |
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292 | g_ptrScoreMatrix = &NUC_SP; |
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293 | |
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294 | g_scoreGapOpen = -400; |
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295 | g_scoreCenter = 0.0; // center pre-added into score mx |
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296 | g_scoreGapExtend = 0.0; |
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297 | |
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298 | g_bNormalizeCounts = false; |
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299 | |
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300 | g_dSmoothScoreCeil = 999.0; // disable |
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301 | g_dMinBestColScore = 90; |
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302 | g_dMinSmoothScore = 90; |
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303 | |
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304 | g_Distance1 = DISTANCE_Kmer4_6; |
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305 | g_Distance2 = DISTANCE_PctIdKimura; |
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306 | } |
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307 | |
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308 | static void SetDefaultsSPN_RNA() |
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309 | { |
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310 | g_ptrScoreMatrix = &NUC_SP; |
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311 | |
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312 | g_scoreGapOpen = -420; |
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313 | g_scoreCenter = -300; // total center = NUC_EXTEND - 300 |
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314 | g_scoreGapExtend = 0.0; |
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315 | |
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316 | g_bNormalizeCounts = false; |
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317 | |
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318 | g_dSmoothScoreCeil = 999.0; // disable |
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319 | g_dMinBestColScore = 90; |
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320 | g_dMinSmoothScore = 90; |
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321 | |
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322 | g_Distance1 = DISTANCE_Kmer4_6; |
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323 | g_Distance2 = DISTANCE_PctIdKimura; |
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324 | } |
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325 | |
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326 | static void FlagParam(const char *OptName, bool *ptrParam, bool bValueIfFlagSet) |
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327 | { |
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328 | bool bIsSet = FlagOpt(OptName); |
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329 | if (bIsSet) |
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330 | *ptrParam = bValueIfFlagSet; |
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331 | } |
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332 | |
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333 | static void StrParam(const char *OptName, const char **ptrptrParam) |
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334 | { |
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335 | const char *opt = ValueOpt(OptName); |
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336 | if (0 != opt) |
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337 | *ptrptrParam = opt; |
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338 | } |
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339 | |
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340 | static void FloatParam(const char *OptName, float *ptrParam) |
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341 | { |
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342 | const char *opt = ValueOpt(OptName); |
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343 | if (0 != opt) |
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344 | *ptrParam = (float) atof(opt); |
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345 | } |
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346 | |
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347 | static void UintParam(const char *OptName, unsigned *ptrParam) |
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348 | { |
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349 | const char *opt = ValueOpt(OptName); |
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350 | if (0 != opt) |
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351 | *ptrParam = atou(opt); |
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352 | } |
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353 | |
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354 | static void EnumParam(const char *OptName, EnumOpt *Opts, int *Param) |
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355 | { |
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356 | const char *Value = ValueOpt(OptName); |
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357 | if (0 == Value) |
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358 | return; |
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359 | |
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360 | for (;;) |
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361 | { |
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362 | if (0 == Opts->pstrOpt) |
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363 | Quit("Invalid parameter -%s %s", OptName, Value); |
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364 | if (0 == stricmp(Value, Opts->pstrOpt)) |
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365 | { |
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366 | *Param = Opts->iValue; |
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367 | return; |
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368 | } |
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369 | ++Opts; |
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370 | } |
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371 | } |
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372 | |
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373 | static void SetPPDefaultParams() |
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374 | { |
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375 | switch (g_PPScore) |
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376 | { |
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377 | case PPSCORE_SP: |
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378 | SetDefaultsSP(); |
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379 | break; |
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380 | |
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381 | case PPSCORE_LE: |
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382 | SetDefaultsLE(); |
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383 | break; |
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384 | |
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385 | case PPSCORE_SV: |
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386 | SetDefaultsSV(); |
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387 | break; |
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388 | |
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389 | case PPSCORE_SPN: |
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390 | switch (g_Alpha) |
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391 | { |
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392 | case ALPHA_DNA: |
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393 | SetDefaultsSPN_DNA(); |
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394 | break; |
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395 | case ALPHA_RNA: |
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396 | SetDefaultsSPN_RNA(); |
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397 | break; |
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398 | default: |
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399 | Quit("Invalid alpha %d", g_Alpha); |
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400 | } |
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401 | break; |
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402 | |
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403 | default: |
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404 | Quit("Invalid g_PPScore"); |
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405 | } |
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406 | } |
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407 | |
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408 | static void SetPPCommandLineParams() |
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409 | { |
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410 | FloatParam("GapOpen", &g_scoreGapOpen); |
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411 | FloatParam("GapOpen2", &g_scoreGapOpen2); |
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412 | FloatParam("GapExtend", &g_scoreGapExtend); |
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413 | FloatParam("GapExtend2", &g_scoreGapExtend2); |
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414 | FloatParam("GapAmbig", &g_scoreAmbigFactor); |
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415 | FloatParam("Center", &g_scoreCenter); |
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416 | FloatParam("SmoothScoreCeil", &g_dSmoothScoreCeil); |
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417 | FloatParam("MinBestColScore", &g_dMinBestColScore); |
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418 | FloatParam("MinSmoothScore", &g_dMinSmoothScore); |
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419 | |
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420 | EnumParam("Distance", DISTANCE_Opts, (int *) &g_Distance1); |
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421 | EnumParam("Distance", DISTANCE_Opts, (int *) &g_Distance2); |
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422 | |
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423 | EnumParam("Distance1", DISTANCE_Opts, (int *) &g_Distance1); |
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424 | EnumParam("Distance2", DISTANCE_Opts, (int *) &g_Distance2); |
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425 | } |
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426 | |
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427 | void SetPPScore(bool bRespectFlagOpts) |
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428 | { |
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429 | if (bRespectFlagOpts) |
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430 | { |
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431 | if (FlagOpt("SP")) |
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432 | g_PPScore = PPSCORE_SP; |
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433 | else if (FlagOpt("LE")) |
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434 | g_PPScore = PPSCORE_LE; |
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435 | else if (FlagOpt("SV")) |
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436 | g_PPScore = PPSCORE_SV; |
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437 | else if (FlagOpt("SPN")) |
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438 | g_PPScore = PPSCORE_SPN; |
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439 | } |
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440 | |
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441 | switch (g_PPScore) |
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442 | { |
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443 | case PPSCORE_LE: |
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444 | case PPSCORE_SP: |
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445 | case PPSCORE_SV: |
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446 | if (ALPHA_RNA == g_Alpha || ALPHA_DNA == g_Alpha) |
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447 | g_PPScore = PPSCORE_SPN; |
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448 | break; |
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449 | case PPSCORE_SPN: |
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450 | if (ALPHA_Amino == g_Alpha) |
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451 | g_PPScore = PPSCORE_LE; |
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452 | break; |
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453 | } |
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454 | |
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455 | SetPPDefaultParams(); |
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456 | SetPPCommandLineParams(); |
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457 | |
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458 | if (g_bVerbose) |
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459 | ListParams(); |
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460 | } |
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461 | |
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462 | void SetPPScore(PPSCORE p) |
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463 | { |
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464 | g_PPScore = p; |
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465 | SetPPScore(true); |
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466 | } |
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467 | |
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468 | static void SetMaxSecs() |
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469 | { |
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470 | float fMaxHours = 0.0; |
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471 | FloatParam("MaxHours", &fMaxHours); |
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472 | if (0.0 == fMaxHours) |
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473 | return; |
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474 | g_ulMaxSecs = (unsigned long) (fMaxHours*60*60); |
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475 | } |
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476 | |
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477 | static bool CanDoLowComplexity() |
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478 | { |
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479 | if (g_SeqWeight1 != SEQWEIGHT_ClustalW) |
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480 | return false; |
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481 | if (1 == g_uMaxIters) |
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482 | return true; |
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483 | return g_SeqWeight2 == SEQWEIGHT_ClustalW; |
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484 | } |
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485 | |
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486 | bool MissingCommand() |
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487 | { |
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488 | if (strcmp(g_pstrInFileName, "-")) |
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489 | return false; |
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490 | if (0 != g_pstrFileName1) |
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491 | return false; |
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492 | if (0 != g_pstrSPFileName) |
---|
493 | return false; |
---|
494 | return true; |
---|
495 | } |
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496 | |
---|
497 | void SetParams() |
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498 | { |
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499 | SetMaxSecs(); |
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500 | |
---|
501 | StrParam("in", &g_pstrInFileName); |
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502 | StrParam("out", &g_pstrOutFileName); |
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503 | |
---|
504 | StrParam("FASTAOut", &g_pstrFASTAOutFileName); |
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505 | StrParam("ClwOut", &g_pstrClwOutFileName); |
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506 | StrParam("ClwStrictOut", &g_pstrClwStrictOutFileName); |
---|
507 | StrParam("HTMLOut", &g_pstrHTMLOutFileName); |
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508 | StrParam("PHYIOut", &g_pstrPHYIOutFileName); |
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509 | StrParam("PHYSOut", &g_pstrPHYSOutFileName); |
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510 | StrParam("MSFOut", &g_pstrMSFOutFileName); |
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511 | |
---|
512 | StrParam("in1", &g_pstrFileName1); |
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513 | StrParam("in2", &g_pstrFileName2); |
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514 | |
---|
515 | StrParam("Matrix", &g_pstrMatrixFileName); |
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516 | StrParam("SPScore", &g_pstrSPFileName); |
---|
517 | |
---|
518 | StrParam("UseTree_NoWarn", &g_pstrUseTreeFileName); |
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519 | if (0 != g_pstrUseTreeFileName) |
---|
520 | g_bUseTreeNoWarn = true; |
---|
521 | |
---|
522 | StrParam("UseTree", &g_pstrUseTreeFileName); |
---|
523 | StrParam("ComputeWeights", &g_pstrComputeWeightsFileName); |
---|
524 | StrParam("ScoreFile", &g_pstrScoreFileName); |
---|
525 | StrParam("DistMx1", &g_pstrDistMxFileName1); |
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526 | StrParam("DistMx2", &g_pstrDistMxFileName2); |
---|
527 | |
---|
528 | FlagParam("Core", &g_bCatchExceptions, false); |
---|
529 | FlagParam("NoCore", &g_bCatchExceptions, true); |
---|
530 | |
---|
531 | FlagParam("Diags1", &g_bDiags1, true); |
---|
532 | FlagParam("Diags2", &g_bDiags2, true); |
---|
533 | |
---|
534 | bool Diags = false; |
---|
535 | FlagParam("Diags", &Diags, true); |
---|
536 | if (Diags) |
---|
537 | { |
---|
538 | g_bDiags1 = true; |
---|
539 | g_bDiags2 = true; |
---|
540 | } |
---|
541 | |
---|
542 | FlagParam("Anchors", &g_bAnchors, true); |
---|
543 | FlagParam("NoAnchors", &g_bAnchors, false); |
---|
544 | |
---|
545 | FlagParam("Quiet", &g_bQuiet, true); |
---|
546 | FlagParam("Verbose", &g_bVerbose, true); |
---|
547 | FlagParam("Version", &g_bVersion, true); |
---|
548 | FlagParam("Stable", &g_bStable, true); |
---|
549 | FlagParam("Group", &g_bStable, false); |
---|
550 | FlagParam("Refine", &g_bRefine, true); |
---|
551 | FlagParam("RefineW", &g_bRefineW, true); |
---|
552 | FlagParam("ProfDB", &g_bProfDB, true); |
---|
553 | FlagParam("SW", &g_bSW, true); |
---|
554 | FlagParam("ClusterOnly", &g_bClusterOnly, true); |
---|
555 | FlagParam("Profile", &g_bProfile, true); |
---|
556 | FlagParam("PPScore", &g_bPPScore, true); |
---|
557 | FlagParam("Brenner", &g_bBrenner, true); |
---|
558 | FlagParam("Dimer", &g_bDimer, true); |
---|
559 | |
---|
560 | FlagParam("MSF", &g_bMSF, true); |
---|
561 | FlagParam("PHYI", &g_bPHYI, true); |
---|
562 | FlagParam("PHYS", &g_bPHYS, true); |
---|
563 | FlagParam("clw", &g_bAln, true); |
---|
564 | FlagParam("HTML", &g_bHTML, true); |
---|
565 | FlagParam("FASTA", &g_bFASTA, true); |
---|
566 | FlagParam("PAS", &g_bPAS, true); |
---|
567 | FlagParam("MakeTree", &g_bMakeTree, true); |
---|
568 | |
---|
569 | if (g_bStable) |
---|
570 | Quit("-stable not supported in this version of muscle"); |
---|
571 | |
---|
572 | bool b = false; |
---|
573 | FlagParam("clwstrict", &b, true); |
---|
574 | if (b) |
---|
575 | { |
---|
576 | g_bAln = true; |
---|
577 | g_bClwStrict = true; |
---|
578 | } |
---|
579 | |
---|
580 | UintParam("MaxIters", &g_uMaxIters); |
---|
581 | UintParam("MaxTrees", &g_uMaxTreeRefineIters); |
---|
582 | UintParam("SmoothWindow", &g_uSmoothWindowLength); |
---|
583 | UintParam("RefineWindow", &g_uRefineWindow); |
---|
584 | UintParam("FromWindow", &g_uWindowFrom); |
---|
585 | UintParam("ToWindow", &g_uWindowTo); |
---|
586 | UintParam("SaveWindow", &g_uSaveWindow); |
---|
587 | UintParam("WindowOffset", &g_uWindowOffset); |
---|
588 | UintParam("AnchorSpacing", &g_uAnchorSpacing); |
---|
589 | UintParam("DiagLength", &g_uMinDiagLength); |
---|
590 | UintParam("DiagMargin", &g_uDiagMargin); |
---|
591 | UintParam("DiagBreak", &g_uMaxDiagBreak); |
---|
592 | UintParam("MaxSubFam", &g_uMaxSubFamCount); |
---|
593 | |
---|
594 | UintParam("Hydro", &g_uHydrophobicRunLength); |
---|
595 | FlagParam("TomHydro", &g_bTomHydro, true); |
---|
596 | if (g_bTomHydro) |
---|
597 | g_uHydrophobicRunLength = 0; |
---|
598 | |
---|
599 | FloatParam("SUEFF", &g_dSUEFF); |
---|
600 | FloatParam("HydroFactor", &g_dHydroFactor); |
---|
601 | |
---|
602 | EnumParam("ObjScore", OBJSCORE_Opts, (int *) &g_ObjScore); |
---|
603 | EnumParam("TermGaps", TERMGAPS_Opts, (int *) &g_TermGaps); |
---|
604 | |
---|
605 | EnumParam("Weight", SEQWEIGHT_Opts, (int *) &g_SeqWeight1); |
---|
606 | EnumParam("Weight", SEQWEIGHT_Opts, (int *) &g_SeqWeight2); |
---|
607 | |
---|
608 | EnumParam("Weight1", SEQWEIGHT_Opts, (int *) &g_SeqWeight1); |
---|
609 | EnumParam("Weight2", SEQWEIGHT_Opts, (int *) &g_SeqWeight2); |
---|
610 | |
---|
611 | EnumParam("Cluster", CLUSTER_Opts, (int *) &g_Cluster1); |
---|
612 | EnumParam("Cluster", CLUSTER_Opts, (int *) &g_Cluster2); |
---|
613 | |
---|
614 | EnumParam("Cluster1", CLUSTER_Opts, (int *) &g_Cluster1); |
---|
615 | EnumParam("Cluster2", CLUSTER_Opts, (int *) &g_Cluster2); |
---|
616 | |
---|
617 | EnumParam("Root1", ROOT_Opts, (int *) &g_Root1); |
---|
618 | EnumParam("Root2", ROOT_Opts, (int *) &g_Root2); |
---|
619 | |
---|
620 | EnumParam("SeqType", SEQTYPE_Opts, (int *) &g_SeqType); |
---|
621 | |
---|
622 | g_scoreGapAmbig = g_scoreGapOpen*g_scoreAmbigFactor; |
---|
623 | g_bLow = CanDoLowComplexity(); |
---|
624 | |
---|
625 | if (g_bDimer) |
---|
626 | g_bPrecompiledCenter = false; |
---|
627 | |
---|
628 | UintParam("MaxMB", &g_uMaxMB); |
---|
629 | if (0 == ValueOpt("MaxMB")) |
---|
630 | g_uMaxMB = (unsigned) (GetRAMSizeMB()*DEFAULT_MAX_MB_FRACT); |
---|
631 | } |
---|