1 | |
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2 | #include "phylip.h" |
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3 | #include "seq.h" |
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4 | |
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5 | /* version 3.6. (c) Copyright 1993-2000 by the University of Washington. |
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6 | Written by Joseph Felsenstein, Lucas Mix, Akiko Fuseki, Sean Lamont, |
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7 | Andrew Keeffe, Dan Fineman, and Patrick Colacurcio. |
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8 | Permission is granted to copy and use this program provided no fee is |
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9 | charged for it and provided that this copyright notice is not removed. */ |
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10 | |
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11 | |
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12 | typedef long vall[maxcategs]; |
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13 | typedef double contribarr[maxcategs]; |
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14 | |
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15 | #ifndef OLDC |
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16 | /* function prototypes */ |
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17 | void init_protmats(void); |
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18 | void getoptions(void); |
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19 | void makeprotfreqs(void); |
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20 | void allocrest(void); |
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21 | void doinit(void); |
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22 | void inputoptions(void); |
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23 | void input_protdata(long); |
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24 | void makeweights(void); |
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25 | void prot_makevalues(long, pointarray, long, long, sequence, steptr); |
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26 | void prot_inittable(void); |
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27 | |
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28 | void alloc_pmatrix(long); |
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29 | void getinput(void); |
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30 | void inittravtree(node *); |
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31 | void prot_nuview(node *); |
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32 | void prot_slopecurv(node *, double, double *, double *, double *); |
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33 | void makenewv(node *); |
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34 | void update(node *); |
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35 | void smooth(node *); |
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36 | void make_pmatrix(double **, double **, double **, long, double, |
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37 | double, double *, double **); |
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38 | double prot_evaluate(node *, boolean); |
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39 | |
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40 | void treevaluate(void); |
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41 | void promlcopy(tree *, tree *, long, long); |
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42 | void proml_re_move(node **, node **); |
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43 | void insert_(node *, node *, boolean); |
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44 | void addtraverse(node *, node *, boolean); |
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45 | void rearrange(node *, node *); |
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46 | void proml_coordinates(node *, double, long *, double *); |
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47 | void proml_printree(void); |
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48 | void sigma(node *, double *, double *, double *); |
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49 | void describe(node *); |
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50 | |
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51 | void prot_reconstr(node *, long); |
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52 | void rectrav(node *, long, long); |
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53 | void summarize(void); |
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54 | void initpromlnode(node **, node **, node *, long, long, long *, long *, |
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55 | initops, pointarray, pointarray, Char *, Char *, FILE *); |
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56 | void dnaml_treeout(node *); |
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57 | void buildnewtip(long, tree *); |
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58 | void buildsimpletree(tree *); |
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59 | void free_all_protx (long, pointarray); |
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60 | void maketree(void); |
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61 | void clean_up(void); |
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62 | |
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63 | /* function prototypes */ |
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64 | #endif |
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65 | |
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66 | extern sequence y; |
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67 | |
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68 | long rcategs; |
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69 | |
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70 | |
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71 | Char infilename[100], outfilename[100], intreename[100], outtreename[100], |
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72 | catfilename[100], weightfilename[100]; |
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73 | double *rate, *rrate, *probcat; |
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74 | long nonodes2, sites, weightsum, categs, |
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75 | datasets, ith, njumble, jumb; |
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76 | long inseed, inseed0; |
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77 | boolean global, jumble, weights, trout, usertree, reconsider, |
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78 | ctgry, rctgry, auto_, hypstate, progress, mulsets, justwts, firstset, |
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79 | improve, smoothit, polishing, lngths, gama, invar, usepam, usejtt; |
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80 | tree curtree, bestree, bestree2, priortree; |
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81 | node *qwhere, *grbg; |
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82 | double cv, alpha, lambda, invarfrac, bestyet; |
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83 | long *enterorder; |
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84 | steptr aliasweight; |
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85 | contribarr *contribution, global_like, global_nulike, global_clai; |
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86 | double **term, **slopeterm, **curveterm; |
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87 | longer seed; |
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88 | char *progname; |
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89 | char aachar[26]="ARNDCQEGHILKMFPSTWYVBZX?*-"; |
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90 | |
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91 | /* Local variables for maketree, propagated globally for c version: */ |
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92 | long nextsp, numtrees, maxwhich, mx, mx0, mx1; |
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93 | double dummy, maxlogl; |
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94 | boolean succeeded, smoothed; |
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95 | double **l0gf; |
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96 | double *l0gl; |
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97 | double **tbl; |
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98 | Char global_ch, ch2; |
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99 | long col; |
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100 | vall *mp; |
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101 | |
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102 | |
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103 | /* Variables introduced to allow for protein probability calculations */ |
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104 | long max_num_sibs; /* maximum number of siblings used in a */ |
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105 | /* nuview calculation. determines size */ |
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106 | /* final size of pmatrices */ |
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107 | double *eigmat; /* eig matrix variable */ |
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108 | double **probmat; /* prob matrix variable */ |
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109 | double ****dpmatrix; /* derivative of pmatrix */ |
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110 | double ****ddpmatrix; /* derivative of xpmatrix */ |
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111 | double *****pmatrices; /* matrix of probabilities of protien */ |
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112 | /* conversion. The 5 subscripts refer */ |
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113 | /* to sibs, rcategs, categs, final and */ |
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114 | /* initial states, respectively. */ |
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115 | double freqaa[20]; /* amino acid frequencies */ |
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116 | |
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117 | static double pameigmat[20] = |
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118 | { |
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119 | -0.022091252,-0.019297602, 0.000004760,-0.017477817, |
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120 | -0.016575549,-0.015504543,-0.002112213,-0.002685727, |
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121 | -0.002976402,-0.013440755,-0.012926992,-0.004293227, |
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122 | -0.005356688,-0.011064786,-0.010480731,-0.008760449, |
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123 | -0.007142318,-0.007381851,-0.007806557,-0.008127024 |
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124 | }; |
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125 | |
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126 | static double pamprobmat[20][20] = |
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127 | { |
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128 | {-0.01522976,-0.00746819,-0.13934468, 0.11755315,-0.00212101, |
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129 | 0.01558456,-0.07408235,-0.00322387, 0.01375826, 0.00448826, |
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130 | 0.00154174, 0.02013313,-0.00159183,-0.00069275,-0.00399898, |
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131 | 0.08414055,-0.01188178,-0.00029870, 0.00220371, 0.00042546}, |
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132 | {-0.07765582,-0.00712634,-0.03683209,-0.08065755,-0.00462872, |
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133 | -0.03791039, 0.10642147,-0.00912185, 0.01436308,-0.00133243, |
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134 | 0.00166346, 0.00624657,-0.00003363,-0.00128729,-0.00690319, |
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135 | 0.17442028,-0.05373336,-0.00078751,-0.00038151, 0.01382718}, |
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136 | {-0.08810973,-0.04081786,-0.04066004,-0.04736004,-0.03275406, |
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137 | -0.03761164,-0.05047487,-0.09086213,-0.03269598,-0.03558015, |
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138 | -0.08407966,-0.07970977,-0.01504743,-0.04011920,-0.05182232, |
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139 | -0.07026991,-0.05846931,-0.01016998,-0.03047472,-0.06280511}, |
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140 | { 0.02513756,-0.00578333, 0.09865453, 0.01322314,-0.00310665, |
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141 | 0.05880899,-0.09252443,-0.02986539,-0.03127460, 0.01007539, |
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142 | -0.00360119,-0.01995024, 0.00094940,-0.00145868,-0.01388816, |
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143 | 0.11358341,-0.12127513,-0.00054696,-0.00055627, 0.00417284}, |
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144 | { 0.16517316,-0.00254742,-0.03318745,-0.01984173, 0.00031890, |
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145 | -0.02817810, 0.02661678,-0.01761215, 0.01665112, 0.10513343, |
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146 | -0.00545026, 0.01827470,-0.00207616,-0.00763758,-0.01322808, |
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147 | -0.02202576,-0.07434204, 0.00020593, 0.00119979,-0.10827873}, |
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148 | { 0.16088826, 0.00056313,-0.02579303,-0.00319655, 0.00037228, |
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149 | -0.03193150, 0.01655305,-0.03028640, 0.01367746,-0.11248153, |
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150 | 0.00778371, 0.02675579, 0.00243718, 0.00895470,-0.01729803, |
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151 | -0.02686964,-0.08262584, 0.00011794,-0.00225134, 0.09415650}, |
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152 | {-0.01739295, 0.00572017,-0.00712592,-0.01100922,-0.00870113, |
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153 | -0.00663461,-0.01153857,-0.02248432,-0.00382264,-0.00358612, |
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154 | -0.00139345,-0.00971460,-0.00133312, 0.01927783,-0.01053838, |
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155 | -0.00911362,-0.01010908, 0.09417598, 0.01763850,-0.00955454}, |
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156 | { 0.01728888, 0.01344211, 0.01200836, 0.01857259,-0.17088517, |
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157 | 0.01457592, 0.01997839, 0.02844884, 0.00839403, 0.00196862, |
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158 | 0.01391984, 0.03270465, 0.00347173,-0.01940984, 0.01233979, |
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159 | 0.00542887, 0.01008836, 0.00126491,-0.02863042, 0.00449764}, |
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160 | {-0.02881366,-0.02184155,-0.01566086,-0.02593764,-0.04050907, |
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161 | -0.01539603,-0.02576729,-0.05089606,-0.00597430, 0.02181643, |
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162 | 0.09835597,-0.04040940, 0.00873512, 0.12139434,-0.02427882, |
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163 | -0.02945238,-0.01566867,-0.01606503, 0.09475319, 0.02238670}, |
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164 | { 0.04080274,-0.02869626,-0.05191093,-0.08435843, 0.00021141, |
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165 | 0.13043842, 0.00871530, 0.00496058,-0.02797641,-0.00636933, |
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166 | 0.02243277, 0.03640362,-0.05735517, 0.00196918,-0.02218934, |
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167 | -0.00608972, 0.02872922, 0.00047619, 0.00151285, 0.00883489}, |
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168 | {-0.02623824, 0.00331152, 0.03640692, 0.04260231,-0.00038223, |
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169 | -0.07480340,-0.01022492,-0.00426473, 0.01448116, 0.01456847, |
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170 | 0.05786680, 0.03368691,-0.10126924,-0.00147454, 0.01275395, |
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171 | 0.00017574,-0.01585206,-0.00015767,-0.00231848, 0.02310137}, |
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172 | {-0.00846258,-0.01508106,-0.01967505,-0.02772004, 0.01248253, |
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173 | -0.01331243,-0.02569382,-0.04461524,-0.02207075, 0.04663443, |
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174 | 0.19347923,-0.02745691, 0.02288515,-0.04883849,-0.01084597, |
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175 | -0.01947187,-0.00081675, 0.00516540,-0.07815919, 0.08035585}, |
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176 | {-0.06553111, 0.09756831, 0.00524326,-0.00885098, 0.00756653, |
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177 | 0.02783099,-0.00427042,-0.16680359, 0.03951331,-0.00490540, |
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178 | 0.01719610, 0.15018204, 0.00882722,-0.00423197,-0.01919217, |
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179 | -0.02963619,-0.01831342,-0.00524338, 0.00011379,-0.02566864}, |
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180 | {-0.07494341,-0.11348850, 0.00241343,-0.00803016, 0.00492438, |
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181 | 0.00711909,-0.00829147, 0.05793337, 0.02734209, 0.02059759, |
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182 | -0.02770280, 0.14128338, 0.01532479, 0.00364307, 0.05968116, |
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183 | -0.06497960,-0.08113941, 0.00319445,-0.00104222, 0.03553497}, |
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184 | { 0.05948223,-0.08959930, 0.03269977,-0.03272374,-0.00365667, |
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185 | -0.03423294,-0.06418925,-0.05902138, 0.05746317,-0.02580596, |
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186 | 0.01259572, 0.05848832, 0.00672666, 0.00233355,-0.05145149, |
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187 | 0.07348503, 0.11427955, 0.00142592,-0.01030651,-0.04862799}, |
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188 | {-0.01606880, 0.05200845,-0.01212967,-0.06824429,-0.00234304, |
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189 | 0.01094203,-0.07375538, 0.08808629, 0.12394822, 0.02231351, |
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190 | -0.03608265,-0.06978045,-0.00618360, 0.00274747,-0.01921876, |
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191 | -0.01541969,-0.02223856,-0.00107603,-0.01251777, 0.05412534}, |
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192 | { 0.01688843, 0.05784728,-0.02256966,-0.07072251,-0.00422551, |
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193 | -0.06261233,-0.08502830, 0.08925346,-0.08529597, 0.01519343, |
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194 | -0.05008258, 0.10931873, 0.00521033, 0.02593305,-0.00717855, |
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195 | 0.02291527, 0.02527388,-0.00266188,-0.00871160, 0.02708135}, |
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196 | {-0.04233344, 0.00076379, 0.01571257, 0.04003092, 0.00901468, |
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197 | 0.00670577, 0.03459487, 0.12420216,-0.00067366,-0.01515094, |
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198 | 0.05306642, 0.04338407, 0.00511287, 0.01036639,-0.17867462, |
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199 | -0.02289440,-0.03213205, 0.00017924,-0.01187362,-0.03933874}, |
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200 | { 0.01284817,-0.01685622, 0.00724363, 0.01687952,-0.00882070, |
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201 | -0.00555957, 0.01676246,-0.05560456,-0.00966893, 0.06197684, |
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202 | -0.09058758, 0.00880607, 0.00108629,-0.08308956,-0.08056832, |
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203 | -0.00413297, 0.02973107, 0.00092948, 0.07010111, 0.13007418}, |
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204 | { 0.00700223,-0.01347574, 0.00691332, 0.03122905, 0.00310308, |
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205 | 0.00946862, 0.03455040,-0.06712536,-0.00304506, 0.04267941, |
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206 | -0.10422292,-0.01127831,-0.00549798, 0.11680505,-0.03352701, |
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207 | -0.00084536, 0.01631369, 0.00095063,-0.09570217, 0.06480321} |
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208 | }; |
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209 | |
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210 | /* this jtt matrix decomposition due to Elisabeth Tillier */ |
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211 | static double jtteigmat[] = |
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212 | {0.0, -0.007031123, -0.006484345, -0.006086499, -0.005514432, |
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213 | -0.00772664, -0.008643413, -0.010620756, -0.009965552, -0.011671808, |
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214 | -0.012222418,-0.004589201, -0.013103714, -0.014048038, -0.003170582, |
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215 | -0.00347935, -0.015311677, -0.016021194, -0.017991454, -0.018911888}; |
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216 | |
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217 | static double jttprobmat[20][20] = |
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218 | {{0.076999996, 0.051000003, 0.043000004, 0.051999998, 0.019999996, 0.041, |
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219 | 0.061999994, 0.073999997, 0.022999999, 0.052000004, 0.090999997, 0.058999988, |
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220 | 0.024000007, 0.04, 0.050999992, 0.069, 0.059000006, 0.014000008, 0.032000004, |
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221 | 0.066000005}, |
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222 | {0.015604455, -0.068062363, 0.020106264, 0.070723273, 0.011702977, 0.009674053, |
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223 | 0.074000798, -0.169750458, 0.005560808, -0.008208636, -0.012305869, |
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224 | -0.063730179, -0.005674643, -0.02116828, 0.104586169, 0.016480839, 0.016765139, |
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225 | 0.005936994, 0.006046367, -0.0082877}, |
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226 | {-0.049778281, -0.007118197, 0.003801272, 0.070749616, 0.047506147, |
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227 | 0.006447017, 0.090522425, -0.053620432, -0.008508175, 0.037170603, |
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228 | 0.051805545, 0.015413608, 0.019939916, -0.008431976, -0.143511376, |
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229 | -0.052486072, -0.032116542, -0.000860626, -0.02535993, 0.03843545}, |
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230 | {-0.028906423, 0.092952047, -0.009615343, -0.067870117, 0.031970392, |
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231 | 0.048338335, -0.054396304, -0.135916654, 0.017780083, 0.000129242, |
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232 | 0.031267424, 0.116333586, 0.007499746, -0.032153596, 0.033517051, |
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233 | -0.013719269, -0.00347293, -0.003291821, -0.02158326, -0.008862168}, |
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234 | {0.037181176, -0.023106564, -0.004482225, -0.029899635, 0.118139633, |
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235 | -0.032298569, -0.04683198, 0.05566988, -0.012622847, 0.002023096, |
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236 | -0.043921088, -0.04792557, -0.003452711, -0.037744513, 0.020822974, |
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237 | 0.036580187, 0.02331425, -0.004807711, -0.017504496, 0.01086673}, |
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238 | {0.044754061, -0.002503471, 0.019452517, -0.015611487, -0.02152807, |
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239 | -0.013131425, -0.03465365, -0.047928912, 0.020608851, 0.067843095, |
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240 | -0.122130014, 0.002521499, 0.013021646, -0.082891087, -0.061590119, |
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241 | 0.016270856, 0.051468938, 0.002079063, 0.081019713, 0.082927944}, |
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242 | {0.058917882, 0.007320741, 0.025278141, 0.000357541, -0.002831285, |
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243 | -0.032453034, -0.010177288, -0.069447924, -0.034467324, 0.011422358, |
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244 | -0.128478324, 0.04309667, -0.015319944, 0.113302422, -0.035052393, |
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245 | 0.046885372, 0.06185183, 0.00175743, -0.06224497, 0.020282093}, |
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246 | {-0.014562092, 0.022522921, -0.007094389, 0.03480089, -0.000326144, |
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247 | -0.124039037, 0.020577906, -0.005056454, -0.081841576, -0.004381786, |
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248 | 0.030826152, 0.091261631, 0.008878828, -0.02829487, 0.042718836, |
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249 | -0.011180886, -0.012719227, -0.000753926, 0.048062375, -0.009399129}, |
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250 | {0.033789571, -0.013512235, 0.088010984, 0.017580292, -0.006608005, |
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251 | -0.037836971, -0.061344686, -0.034268357, 0.018190209, -0.068484614, |
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252 | 0.120024744, -0.00319321, -0.001349477, -0.03000546, -0.073063759, |
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253 | 0.081912399, 0.0635245, 0.000197, -0.002481798, -0.09108114}, |
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254 | {-0.113947615, 0.019230545, 0.088819683, 0.064832765, 0.001801467, |
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255 | -0.063829682, -0.072001633, 0.018429333, 0.057465965, 0.043901014, |
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256 | -0.048050874, -0.001705918, 0.022637173, 0.017404665, 0.043877902, |
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257 | -0.017089594, -0.058489485, 0.000127498, -0.029357194, 0.025943972}, |
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258 | {0.01512923, 0.023603725, 0.006681954, 0.012360216, -0.000181447, |
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259 | -0.023011838, -0.008960024, -0.008533239, 0.012569835, 0.03216118, |
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260 | 0.061986403, -0.001919083, -0.1400832, -0.010669741, -0.003919454, |
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261 | -0.003707024, -0.026806029, -0.000611603, -0.001402648, 0.065312824}, |
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262 | {-0.036405351, 0.020816769, 0.011408213, 0.019787053, 0.038897829, |
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263 | 0.017641789, 0.020858533, -0.006067252, 0.028617353, -0.064259496, |
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264 | -0.081676567, 0.024421823, -0.028751676, 0.07095096, -0.024199434, |
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265 | -0.007513119, -0.028108766, -0.01198095, 0.111761119, -0.076198809}, |
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266 | {0.060831772, 0.144097327, -0.069151377, 0.023754576, -0.003322955, |
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267 | -0.071618574, 0.03353154, -0.02795295, 0.039519769, -0.023453968, |
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268 | -0.000630308, -0.098024591, 0.017672997, 0.003813378, -0.009266499, |
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269 | -0.011192111, 0.016013873, -0.002072968, -0.010022044, -0.012526904}, |
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270 | {-0.050776604, 0.092833081, 0.044069596, 0.050523021, -0.002628417, |
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271 | 0.076542572, -0.06388631, -0.00854892, -0.084725311, 0.017401063, |
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272 | -0.006262541, -0.094457679, -0.002818678, -0.0044122, -0.002883973, |
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273 | 0.028729685, -0.004961596, -0.001498627, 0.017994575, -0.000232779}, |
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274 | {-0.01894566, -0.007760205, -0.015160993, -0.027254587, 0.009800903, |
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275 | -0.013443561, -0.032896517, -0.022734138, -0.001983861, 0.00256111, |
---|
276 | 0.024823166, -0.021256768, 0.001980052, 0.028136263, -0.012364384, |
---|
277 | -0.013782446, -0.013061091, 0.111173981, 0.021702122, 0.00046654}, |
---|
278 | {-0.009444193, -0.042106824, -0.02535015, -0.055125574, 0.006369612, |
---|
279 | -0.02945416, -0.069922064, -0.067221068, -0.003004999, 0.053624311, |
---|
280 | 0.128862984, -0.057245803, 0.025550508, 0.087741073, -0.001119043, |
---|
281 | -0.012036202, -0.000913488, -0.034864475, 0.050124813, 0.055534723}, |
---|
282 | {0.145782464, -0.024348311, -0.031216873, 0.106174443, 0.00202862, |
---|
283 | 0.02653866, -0.113657267, -0.00755018, 0.000307232, -0.051241158, |
---|
284 | 0.001310685, 0.035275877, 0.013308898, 0.002957626, -0.002925034, |
---|
285 | -0.065362319, -0.071844582, 0.000475894, -0.000112419, 0.034097762}, |
---|
286 | {0.079840455, 0.018769331, 0.078685899, -0.084329807, -0.00277264, |
---|
287 | -0.010099754, 0.059700608, -0.019209715, -0.010442992, -0.042100476, |
---|
288 | -0.006020556, -0.023061786, 0.017246106, -0.001572858, -0.006703785, |
---|
289 | 0.056301316, -0.156787357, -0.000303638, 0.001498195, 0.051363455}, |
---|
290 | {0.049628261, 0.016475144, 0.094141653, -0.04444633, 0.005206131, |
---|
291 | -0.001827555, 0.02195624, 0.013066683, -0.010415582, -0.022338403, |
---|
292 | 0.007837197, -0.023397671, -0.002507095, 0.005177694, 0.017109561, |
---|
293 | -0.202340113, 0.069681441, 0.000120736, 0.002201146, 0.004670849}, |
---|
294 | {0.089153689, 0.000233354, 0.010826822, -0.004273519, 0.001440618, |
---|
295 | 0.000436077, 0.001182351, -0.002255508, -0.000700465, 0.150589876, |
---|
296 | -0.003911914, -0.00050154, -0.004564983, 0.00012701, -0.001486973, |
---|
297 | -0.018902754, -0.054748555, 0.000217377, -0.000319302, -0.162541651}}; |
---|
298 | |
---|
299 | void init_protmats() |
---|
300 | { |
---|
301 | long l, m; |
---|
302 | |
---|
303 | eigmat = (double *) Malloc (20 * sizeof(double)); |
---|
304 | for (l = 0; l <= 19; l++) |
---|
305 | if (usejtt) |
---|
306 | eigmat[l] = jtteigmat[l]; |
---|
307 | else |
---|
308 | eigmat[l] = pameigmat[l]; |
---|
309 | probmat = (double **) Malloc (20 * sizeof(double *)); |
---|
310 | for (l = 0; l < 20; l++) |
---|
311 | probmat[l] = (double *) Malloc (20 * sizeof(double)); |
---|
312 | for (l = 0; l <= 19; l++) |
---|
313 | for (m= 0; m <= 19; m++) |
---|
314 | if (usejtt) |
---|
315 | probmat[l][m] = jttprobmat[l][m]; |
---|
316 | else |
---|
317 | probmat[l][m] = pamprobmat[l][m]; |
---|
318 | } /* init_protmats */ |
---|
319 | |
---|
320 | |
---|
321 | void getoptions() |
---|
322 | { |
---|
323 | /* interactively set options */ |
---|
324 | long i, loopcount, loopcount2; |
---|
325 | Char ch; |
---|
326 | boolean didchangecat, didchangercat; |
---|
327 | double probsum; |
---|
328 | |
---|
329 | fprintf(outfile, "\nAmino acid sequence Maximum Likelihood"); |
---|
330 | fprintf(outfile, " method, version %s\n\n",VERSION); |
---|
331 | putchar('\n'); |
---|
332 | ctgry = false; |
---|
333 | didchangecat = false; |
---|
334 | rctgry = false; |
---|
335 | didchangercat = false; |
---|
336 | categs = 1; |
---|
337 | rcategs = 1; |
---|
338 | auto_ = false; |
---|
339 | gama = false; |
---|
340 | global = false; |
---|
341 | hypstate = false; |
---|
342 | improve = false; |
---|
343 | invar = false; |
---|
344 | jumble = false; |
---|
345 | njumble = 1; |
---|
346 | lngths = false; |
---|
347 | lambda = 1.0; |
---|
348 | outgrno = 1; |
---|
349 | outgropt = false; |
---|
350 | reconsider = false; |
---|
351 | trout = true; |
---|
352 | usertree = false; |
---|
353 | weights = false; |
---|
354 | printdata = false; |
---|
355 | progress = true; |
---|
356 | treeprint = true; |
---|
357 | usepam = false; |
---|
358 | usejtt = true; |
---|
359 | interleaved = true; |
---|
360 | init_protmats(); |
---|
361 | loopcount = 0; |
---|
362 | for (;;){ |
---|
363 | cleerhome(); |
---|
364 | printf("Amino acid sequence Maximum Likelihood"); |
---|
365 | printf(" method, version %s\n\n",VERSION); |
---|
366 | printf("Settings for this run:\n"); |
---|
367 | printf(" U Search for best tree? %s\n", |
---|
368 | (usertree ? "No, use user trees in input file" : "Yes")); |
---|
369 | if (usertree) { |
---|
370 | printf(" L Use lengths from user trees? %s\n", |
---|
371 | (lngths ? "Yes" : "No")); |
---|
372 | } |
---|
373 | printf(" P JTT or PAM amino acid change model? %s\n", |
---|
374 | (usejtt ? "Jones-Taylor-Thornton model" : "Dayhoff PAM model")); |
---|
375 | printf(" C One category of sites?"); |
---|
376 | if (!ctgry || categs == 1) |
---|
377 | printf(" Yes\n"); |
---|
378 | else |
---|
379 | printf(" %ld categories of sites\n", categs); |
---|
380 | printf(" R Rate variation among sites?"); |
---|
381 | if (!rctgry) |
---|
382 | printf(" constant rate of change\n"); |
---|
383 | else { |
---|
384 | if (gama) |
---|
385 | printf(" Gamma distributed rates\n"); |
---|
386 | else { |
---|
387 | if (invar) |
---|
388 | printf(" Gamma+Invariant sites\n"); |
---|
389 | else |
---|
390 | printf(" user-defined HMM of rates\n"); |
---|
391 | } |
---|
392 | printf(" A Rates at adjacent sites correlated?"); |
---|
393 | if (!auto_) |
---|
394 | printf(" No, they are independent\n"); |
---|
395 | else |
---|
396 | printf(" Yes, mean block length =%6.1f\n", 1.0 / lambda); |
---|
397 | } |
---|
398 | printf(" W Sites weighted? %s\n", |
---|
399 | (weights ? "Yes" : "No")); |
---|
400 | if ((!usertree) || reconsider) { |
---|
401 | printf(" S Speedier but rougher analysis? %s\n", |
---|
402 | (improve ? "No, not rough" : "Yes")); |
---|
403 | printf(" G Global rearrangements? %s\n", |
---|
404 | (global ? "Yes" : "No")); |
---|
405 | } |
---|
406 | if (!usertree) { |
---|
407 | printf(" J Randomize input order of sequences?"); |
---|
408 | if (jumble) |
---|
409 | printf(" Yes (seed =%8ld,%3ld times)\n", inseed0, njumble); |
---|
410 | else |
---|
411 | printf(" No. Use input order\n"); |
---|
412 | } |
---|
413 | else |
---|
414 | printf(" V Rearrange starting with user tree? %s\n", |
---|
415 | (reconsider ? "Yes" : "No")); |
---|
416 | printf(" O Outgroup root? %s%3ld\n", |
---|
417 | (outgropt ? "Yes, at sequence number" : |
---|
418 | "No, use as outgroup species"),outgrno); |
---|
419 | printf(" M Analyze multiple data sets?"); |
---|
420 | if (mulsets) |
---|
421 | printf(" Yes, %2ld %s\n", datasets, |
---|
422 | (justwts ? "sets of weights" : "data sets")); |
---|
423 | else |
---|
424 | printf(" No\n"); |
---|
425 | printf(" I Input sequences interleaved? %s\n", |
---|
426 | (interleaved ? "Yes" : "No, sequential")); |
---|
427 | printf(" 0 Terminal type (IBM PC, ANSI, none)? %s\n", |
---|
428 | (ibmpc ? "IBM PC" : ansi ? "ANSI" : "(none)")); |
---|
429 | printf(" 1 Print out the data at start of run %s\n", |
---|
430 | (printdata ? "Yes" : "No")); |
---|
431 | printf(" 2 Print indications of progress of run %s\n", |
---|
432 | (progress ? "Yes" : "No")); |
---|
433 | printf(" 3 Print out tree %s\n", |
---|
434 | (treeprint ? "Yes" : "No")); |
---|
435 | printf(" 4 Write out trees onto tree file? %s\n", |
---|
436 | (trout ? "Yes" : "No")); |
---|
437 | printf(" 5 Reconstruct hypothetical sequences? %s\n", |
---|
438 | (hypstate ? "Yes" : "No")); |
---|
439 | printf("\n Y to accept these or type the letter for one to change\n"); |
---|
440 | #ifdef WIN32 |
---|
441 | phyFillScreenColor(); |
---|
442 | #endif |
---|
443 | scanf("%c%*[^\n]", &ch); |
---|
444 | getchar(); |
---|
445 | if (ch == '\n') |
---|
446 | ch = ' '; |
---|
447 | uppercase(&ch); |
---|
448 | if (ch == 'Y') |
---|
449 | break; |
---|
450 | if (strchr("UPLCRAWSGJVOMI012345",ch) != NULL){ |
---|
451 | switch (ch) { |
---|
452 | |
---|
453 | case 'C': |
---|
454 | ctgry = !ctgry; |
---|
455 | if (ctgry) { |
---|
456 | printf("\nSitewise user-assigned categories:\n\n"); |
---|
457 | initcatn(&categs); |
---|
458 | if (rate){ |
---|
459 | free(rate); |
---|
460 | } |
---|
461 | rate = (double *) Malloc(categs * sizeof(double)); |
---|
462 | didchangecat = true; |
---|
463 | initcategs(categs, rate); |
---|
464 | } |
---|
465 | break; |
---|
466 | |
---|
467 | case 'P': |
---|
468 | if (usejtt) { |
---|
469 | usejtt = false; |
---|
470 | usepam = true; |
---|
471 | } else { |
---|
472 | usepam = false; |
---|
473 | usejtt = true; |
---|
474 | } |
---|
475 | break; |
---|
476 | |
---|
477 | case 'R': |
---|
478 | if (!rctgry) { |
---|
479 | rctgry = true; |
---|
480 | gama = true; |
---|
481 | } else { |
---|
482 | if (gama) { |
---|
483 | gama = false; |
---|
484 | invar = true; |
---|
485 | } else { |
---|
486 | if (invar) |
---|
487 | invar = false; |
---|
488 | else |
---|
489 | rctgry = false; |
---|
490 | } |
---|
491 | } |
---|
492 | break; |
---|
493 | |
---|
494 | case 'A': |
---|
495 | auto_ = !auto_; |
---|
496 | if (auto_) |
---|
497 | initlambda(&lambda); |
---|
498 | break; |
---|
499 | |
---|
500 | case 'W': |
---|
501 | weights = !weights; |
---|
502 | break; |
---|
503 | |
---|
504 | case 'S': |
---|
505 | improve = !improve; |
---|
506 | break; |
---|
507 | |
---|
508 | case 'G': |
---|
509 | global = !global; |
---|
510 | break; |
---|
511 | |
---|
512 | case 'J': |
---|
513 | jumble = !jumble; |
---|
514 | if (jumble) |
---|
515 | initjumble(&inseed, &inseed0, seed, &njumble); |
---|
516 | else njumble = 1; |
---|
517 | break; |
---|
518 | |
---|
519 | case 'L': |
---|
520 | lngths = !lngths; |
---|
521 | break; |
---|
522 | |
---|
523 | case 'O': |
---|
524 | outgropt = !outgropt; |
---|
525 | if (outgropt) |
---|
526 | initoutgroup(&outgrno, spp); |
---|
527 | break; |
---|
528 | |
---|
529 | case 'U': |
---|
530 | usertree = !usertree; |
---|
531 | break; |
---|
532 | |
---|
533 | case 'V': |
---|
534 | reconsider = !reconsider; |
---|
535 | break; |
---|
536 | |
---|
537 | case 'M': |
---|
538 | mulsets = !mulsets; |
---|
539 | if (mulsets) { |
---|
540 | printf("Multiple data sets or multiple weights?"); |
---|
541 | loopcount2 = 0; |
---|
542 | do { |
---|
543 | printf(" (type D or W)\n"); |
---|
544 | #ifdef WIN32 |
---|
545 | phyFillScreenColor(); |
---|
546 | #endif |
---|
547 | scanf("%c%*[^\n]", &ch2); |
---|
548 | getchar(); |
---|
549 | if (ch2 == '\n') |
---|
550 | ch2 = ' '; |
---|
551 | uppercase(&ch2); |
---|
552 | countup(&loopcount2, 10); |
---|
553 | } while ((ch2 != 'W') && (ch2 != 'D')); |
---|
554 | justwts = (ch2 == 'W'); |
---|
555 | if (justwts) |
---|
556 | justweights(&datasets); |
---|
557 | else |
---|
558 | initdatasets(&datasets); |
---|
559 | if (!jumble) { |
---|
560 | jumble = true; |
---|
561 | initjumble(&inseed, &inseed0, seed, &njumble); |
---|
562 | } |
---|
563 | } |
---|
564 | break; |
---|
565 | |
---|
566 | case 'I': |
---|
567 | interleaved = !interleaved; |
---|
568 | break; |
---|
569 | |
---|
570 | case '0': |
---|
571 | initterminal(&ibmpc, &ansi); |
---|
572 | break; |
---|
573 | |
---|
574 | case '1': |
---|
575 | printdata = !printdata; |
---|
576 | break; |
---|
577 | |
---|
578 | case '2': |
---|
579 | progress = !progress; |
---|
580 | break; |
---|
581 | |
---|
582 | case '3': |
---|
583 | treeprint = !treeprint; |
---|
584 | break; |
---|
585 | |
---|
586 | case '4': |
---|
587 | trout = !trout; |
---|
588 | break; |
---|
589 | |
---|
590 | case '5': |
---|
591 | hypstate = !hypstate; |
---|
592 | break; |
---|
593 | } |
---|
594 | } else |
---|
595 | printf("Not a possible option!\n"); |
---|
596 | countup(&loopcount, 100); |
---|
597 | } |
---|
598 | if (gama || invar) { |
---|
599 | loopcount = 0; |
---|
600 | do { |
---|
601 | printf( |
---|
602 | "\nCoefficient of variation of substitution rate among sites (must be positive)\n"); |
---|
603 | printf( |
---|
604 | " In gamma distribution parameters, this is 1/(square root of alpha)\n"); |
---|
605 | #ifdef WIN32 |
---|
606 | phyFillScreenColor(); |
---|
607 | #endif |
---|
608 | scanf("%lf%*[^\n]", &cv); |
---|
609 | getchar(); |
---|
610 | countup(&loopcount, 10); |
---|
611 | } while (cv <= 0.0); |
---|
612 | alpha = 1.0 / (cv * cv); |
---|
613 | } |
---|
614 | if (!rctgry) |
---|
615 | auto_ = false; |
---|
616 | if (rctgry) { |
---|
617 | printf("\nRates in HMM"); |
---|
618 | if (invar) |
---|
619 | printf(" (including one for invariant sites)"); |
---|
620 | printf(":\n"); |
---|
621 | initcatn(&rcategs); |
---|
622 | if (probcat){ |
---|
623 | free(probcat); |
---|
624 | free(rrate); |
---|
625 | } |
---|
626 | probcat = (double *) Malloc(rcategs * sizeof(double)); |
---|
627 | rrate = (double *) Malloc(rcategs * sizeof(double)); |
---|
628 | didchangercat = true; |
---|
629 | if (gama) |
---|
630 | initgammacat(rcategs, alpha, rrate, probcat); |
---|
631 | else { |
---|
632 | if (invar) { |
---|
633 | loopcount = 0; |
---|
634 | do { |
---|
635 | printf("Fraction of invariant sites?\n"); |
---|
636 | scanf("%lf%*[^\n]", &invarfrac); |
---|
637 | getchar(); |
---|
638 | countup (&loopcount, 10); |
---|
639 | } while ((invarfrac <= 0.0) || (invarfrac >= 1.0)); |
---|
640 | initgammacat(rcategs-1, alpha, rrate, probcat); |
---|
641 | for (i = 0; i < rcategs-1; i++) |
---|
642 | probcat[i] = probcat[i]*(1.0-invarfrac); |
---|
643 | probcat[rcategs-1] = invarfrac; |
---|
644 | rrate[rcategs-1] = 0.0; |
---|
645 | } else { |
---|
646 | initcategs(rcategs, rrate); |
---|
647 | initprobcat(rcategs, &probsum, probcat); |
---|
648 | } |
---|
649 | } |
---|
650 | } |
---|
651 | if (!didchangercat){ |
---|
652 | rrate = (double *) Malloc(rcategs*sizeof(double)); |
---|
653 | probcat = (double *) Malloc(rcategs*sizeof(double)); |
---|
654 | rrate[0] = 1.0; |
---|
655 | probcat[0] = 1.0; |
---|
656 | } |
---|
657 | if (!didchangecat) { |
---|
658 | rate = (double *) Malloc(categs*sizeof(double)); |
---|
659 | rate[0] = 1.0; |
---|
660 | } |
---|
661 | } /* getoptions */ |
---|
662 | |
---|
663 | |
---|
664 | void makeprotfreqs() |
---|
665 | { |
---|
666 | /* calculate amino acid frequencies based on eigmat */ |
---|
667 | long i, mineig; |
---|
668 | |
---|
669 | mineig = 0; |
---|
670 | for (i = 0; i <= 19; i++) |
---|
671 | if (fabs(eigmat[i]) < fabs(eigmat[mineig])) |
---|
672 | mineig = i; |
---|
673 | memcpy(freqaa, probmat[mineig], 20 * sizeof(double)); |
---|
674 | for (i = 0; i <= 19; i++) |
---|
675 | freqaa[i] = fabs(freqaa[i]); |
---|
676 | } /* makeprotfreqs */ |
---|
677 | |
---|
678 | |
---|
679 | void allocrest() |
---|
680 | { |
---|
681 | long i; |
---|
682 | |
---|
683 | y = (Char **) Malloc(spp*sizeof(Char *)); |
---|
684 | for (i = 0; i < spp; i++) |
---|
685 | y[i] = (Char *) Malloc(sites*sizeof(Char)); |
---|
686 | nayme = (naym *) Malloc(spp*sizeof(naym)); |
---|
687 | enterorder = (long *) Malloc(spp*sizeof(long)); |
---|
688 | category = (long *) Malloc(sites*sizeof(long)); |
---|
689 | weight = (long *) Malloc(sites*sizeof(long)); |
---|
690 | alias = (long *) Malloc(sites*sizeof(long)); |
---|
691 | ally = (long *) Malloc(sites*sizeof(long)); |
---|
692 | location = (long *) Malloc(sites*sizeof(long)); |
---|
693 | aliasweight = (long *) Malloc(sites*sizeof(long)); |
---|
694 | } /* allocrest */ |
---|
695 | |
---|
696 | |
---|
697 | void doinit() |
---|
698 | { /* initializes variables */ |
---|
699 | inputnumbers(&spp, &sites, &nonodes2, 2); |
---|
700 | getoptions(); |
---|
701 | makeprotfreqs(); |
---|
702 | if (printdata) |
---|
703 | fprintf(outfile, "%2ld species, %3ld sites\n", spp, sites); |
---|
704 | alloctree(&curtree.nodep, nonodes2, usertree); |
---|
705 | allocrest(); |
---|
706 | if (usertree && !reconsider) |
---|
707 | return; |
---|
708 | alloctree(&bestree.nodep, nonodes2, 0); |
---|
709 | alloctree(&priortree.nodep, nonodes2, 0); |
---|
710 | if (njumble <= 1) |
---|
711 | return; |
---|
712 | alloctree(&bestree2.nodep, nonodes2, 0); |
---|
713 | } /* doinit */ |
---|
714 | |
---|
715 | |
---|
716 | void inputoptions() |
---|
717 | { |
---|
718 | long i; |
---|
719 | |
---|
720 | if (!firstset) |
---|
721 | samenumsp(&sites, ith); |
---|
722 | if (firstset) { |
---|
723 | for (i = 0; i < sites; i++) |
---|
724 | category[i] = 1; |
---|
725 | for (i = 0; i < sites; i++) |
---|
726 | weight[i] = 1; |
---|
727 | } |
---|
728 | if (justwts || weights) |
---|
729 | inputweights(sites, weight, &weights); |
---|
730 | weightsum = 0; |
---|
731 | for (i = 0; i < sites; i++) |
---|
732 | weightsum += weight[i]; |
---|
733 | if ((ctgry && categs > 1) && (firstset || !justwts)) { |
---|
734 | inputcategs(0, sites, category, categs, "ProML"); |
---|
735 | if (printdata) |
---|
736 | printcategs(outfile, sites, category, "Site categories"); |
---|
737 | } |
---|
738 | if (weights && printdata) |
---|
739 | printweights(outfile, 0, sites, weight, "Sites"); |
---|
740 | fprintf(outfile, "%s model of amino acid change\n\n", |
---|
741 | (usejtt ? "Jones-Taylor-Thornton" : "Dayhoff PAM")); |
---|
742 | } /* inputoptions */ |
---|
743 | |
---|
744 | |
---|
745 | void input_protdata(long chars) |
---|
746 | { |
---|
747 | /* input the names and sequences for each species */ |
---|
748 | /* used by proml */ |
---|
749 | long i, j, k, l, basesread, basesnew; |
---|
750 | Char charstate; |
---|
751 | boolean allread, done; |
---|
752 | |
---|
753 | if (printdata) |
---|
754 | headings(chars, "Sequences", "---------"); |
---|
755 | basesread = 0; |
---|
756 | basesnew = 0; |
---|
757 | allread = false; |
---|
758 | while (!(allread)) { |
---|
759 | allread = true; |
---|
760 | if (eoln(infile)) |
---|
761 | scan_eoln(infile); |
---|
762 | i = 1; |
---|
763 | while (i <= spp) { |
---|
764 | if ((interleaved && basesread == 0) || !interleaved) |
---|
765 | initname(i - 1); |
---|
766 | j = (interleaved) ? basesread : 0; |
---|
767 | done = false; |
---|
768 | while (!done && !eoff(infile)) { |
---|
769 | if (interleaved) |
---|
770 | done = true; |
---|
771 | while (j < chars && !(eoln(infile) || eoff(infile))) { |
---|
772 | charstate = gettc(infile); |
---|
773 | if (charstate == '\n') |
---|
774 | charstate = ' '; |
---|
775 | if (charstate == ' ' || (charstate >= '0' && charstate <= '9')) |
---|
776 | continue; |
---|
777 | uppercase(&charstate); |
---|
778 | if ((strchr("ABCDEFGHIKLMNPQRSTVWXYZ*?-", charstate)) == NULL) { |
---|
779 | printf("ERROR: bad amino acid: %c at position %ld of species %ld\n", |
---|
780 | charstate, j+1, i); |
---|
781 | if (charstate == '.') { |
---|
782 | printf(" Periods (.) may not be used as gap characters.\n"); |
---|
783 | printf(" The correct gap character is (-)\n"); |
---|
784 | } |
---|
785 | exxit(-1); |
---|
786 | } |
---|
787 | j++; |
---|
788 | y[i - 1][j - 1] = charstate; |
---|
789 | } |
---|
790 | if (interleaved) |
---|
791 | continue; |
---|
792 | if (j < chars) |
---|
793 | scan_eoln(infile); |
---|
794 | else if (j == chars) |
---|
795 | done = true; |
---|
796 | } |
---|
797 | if (interleaved && i == 1) |
---|
798 | basesnew = j; |
---|
799 | |
---|
800 | scan_eoln(infile); |
---|
801 | |
---|
802 | if ((interleaved && j != basesnew) || |
---|
803 | (!interleaved && j != chars)) { |
---|
804 | printf("ERROR: SEQUENCES OUT OF ALIGNMENT AT POSITION %ld.\n", j); |
---|
805 | exxit(-1); |
---|
806 | } |
---|
807 | i++; |
---|
808 | } |
---|
809 | |
---|
810 | if (interleaved) { |
---|
811 | basesread = basesnew; |
---|
812 | allread = (basesread == chars); |
---|
813 | } else |
---|
814 | allread = (i > spp); |
---|
815 | } |
---|
816 | if (!printdata) |
---|
817 | return; |
---|
818 | for (i = 1; i <= ((chars - 1) / 60 + 1); i++) { |
---|
819 | for (j = 1; j <= spp; j++) { |
---|
820 | for (k = 0; k < nmlngth; k++) |
---|
821 | putc(nayme[j - 1][k], outfile); |
---|
822 | fprintf(outfile, " "); |
---|
823 | l = i * 60; |
---|
824 | if (l > chars) |
---|
825 | l = chars; |
---|
826 | for (k = (i - 1) * 60 + 1; k <= l; k++) { |
---|
827 | if (j > 1 && y[j - 1][k - 1] == y[0][k - 1]) |
---|
828 | charstate = '.'; |
---|
829 | else |
---|
830 | charstate = y[j - 1][k - 1]; |
---|
831 | putc(charstate, outfile); |
---|
832 | if (k % 10 == 0 && k % 60 != 0) |
---|
833 | putc(' ', outfile); |
---|
834 | } |
---|
835 | putc('\n', outfile); |
---|
836 | } |
---|
837 | putc('\n', outfile); |
---|
838 | } |
---|
839 | putc('\n', outfile); |
---|
840 | } /* input_protdata */ |
---|
841 | |
---|
842 | |
---|
843 | void makeweights() |
---|
844 | { |
---|
845 | /* make up weights vector to avoid duplicate computations */ |
---|
846 | long i; |
---|
847 | |
---|
848 | for (i = 1; i <= sites; i++) { |
---|
849 | alias[i - 1] = i; |
---|
850 | ally[i - 1] = 0; |
---|
851 | aliasweight[i - 1] = weight[i - 1]; |
---|
852 | location[i - 1] = 0; |
---|
853 | } |
---|
854 | sitesort2 (sites, aliasweight); |
---|
855 | sitecombine2(sites, aliasweight); |
---|
856 | sitescrunch2(sites, 1, 2, aliasweight); |
---|
857 | for (i = 1; i <= sites; i++) { |
---|
858 | if (aliasweight[i - 1] > 0) |
---|
859 | endsite = i; |
---|
860 | } |
---|
861 | for (i = 1; i <= endsite; i++) { |
---|
862 | location[alias[i - 1] - 1] = i; |
---|
863 | ally[alias[i - 1] - 1] = alias[i - 1]; |
---|
864 | } |
---|
865 | term = (double **) Malloc(endsite * sizeof(double *)); |
---|
866 | for (i = 0; i < endsite; i++) |
---|
867 | term[i] = (double *) Malloc(rcategs * sizeof(double)); |
---|
868 | slopeterm = (double **) Malloc(endsite * sizeof(double *)); |
---|
869 | for (i = 0; i < endsite; i++) |
---|
870 | slopeterm[i] = (double *) Malloc(rcategs * sizeof(double)); |
---|
871 | curveterm = (double **) Malloc(endsite * sizeof(double *)); |
---|
872 | for (i = 0; i < endsite; i++) |
---|
873 | curveterm[i] = (double *) Malloc(rcategs * sizeof(double)); |
---|
874 | mp = (vall *) Malloc(sites*sizeof(vall)); |
---|
875 | contribution = (contribarr *) Malloc(endsite*sizeof(contribarr)); |
---|
876 | } /* makeweights */ |
---|
877 | |
---|
878 | |
---|
879 | void prot_makevalues(long local_categs, pointarray treenode, long local_endsite, |
---|
880 | long local_spp, sequence local_y, steptr local_alias) |
---|
881 | { |
---|
882 | /* set up fractional likelihoods at tips */ |
---|
883 | /* a version of makevalues2 found in seq.c */ |
---|
884 | /* used by proml */ |
---|
885 | long i, j, k, l; |
---|
886 | long b; |
---|
887 | |
---|
888 | for (k = 0; k < local_endsite; k++) { |
---|
889 | j = local_alias[k]; |
---|
890 | for (i = 0; i < local_spp; i++) { |
---|
891 | for (l = 0; l < local_categs; l++) { |
---|
892 | memset(treenode[i]->protx[k][l], 0, sizeof(double)*20); |
---|
893 | switch (local_y[i][j - 1]) { |
---|
894 | |
---|
895 | case 'A': |
---|
896 | treenode[i]->protx[k][l][0] = 1.0; |
---|
897 | break; |
---|
898 | |
---|
899 | case 'R': |
---|
900 | treenode[i]->protx[k][l][(long)arginine - (long)alanine] = 1.0; |
---|
901 | break; |
---|
902 | |
---|
903 | case 'N': |
---|
904 | treenode[i]->protx[k][l][(long)asparagine - (long)alanine] = 1.0; |
---|
905 | break; |
---|
906 | |
---|
907 | case 'D': |
---|
908 | treenode[i]->protx[k][l][(long)aspartic - (long)alanine] = 1.0; |
---|
909 | break; |
---|
910 | |
---|
911 | case 'C': |
---|
912 | treenode[i]->protx[k][l][(long)cysteine - (long)alanine] = 1.0; |
---|
913 | break; |
---|
914 | |
---|
915 | case 'Q': |
---|
916 | treenode[i]->protx[k][l][(long)glutamine - (long)alanine] = 1.0; |
---|
917 | break; |
---|
918 | |
---|
919 | case 'E': |
---|
920 | treenode[i]->protx[k][l][(long)glutamic - (long)alanine] = 1.0; |
---|
921 | break; |
---|
922 | |
---|
923 | case 'G': |
---|
924 | treenode[i]->protx[k][l][(long)glycine - (long)alanine] = 1.0; |
---|
925 | break; |
---|
926 | |
---|
927 | case 'H': |
---|
928 | treenode[i]->protx[k][l][(long)histidine - (long)alanine] = 1.0; |
---|
929 | break; |
---|
930 | |
---|
931 | case 'I': |
---|
932 | treenode[i]->protx[k][l][(long)isoleucine - (long)alanine] = 1.0; |
---|
933 | break; |
---|
934 | |
---|
935 | case 'L': |
---|
936 | treenode[i]->protx[k][l][(long)leucine - (long)alanine] = 1.0; |
---|
937 | break; |
---|
938 | |
---|
939 | case 'K': |
---|
940 | treenode[i]->protx[k][l][(long)lysine - (long)alanine] = 1.0; |
---|
941 | break; |
---|
942 | |
---|
943 | case 'M': |
---|
944 | treenode[i]->protx[k][l][(long)methionine - (long)alanine] = 1.0; |
---|
945 | break; |
---|
946 | |
---|
947 | case 'F': |
---|
948 | treenode[i]->protx[k][l][(long)phenylalanine - (long)alanine] = 1.0; |
---|
949 | break; |
---|
950 | |
---|
951 | case 'P': |
---|
952 | treenode[i]->protx[k][l][(long)proline - (long)alanine] = 1.0; |
---|
953 | break; |
---|
954 | |
---|
955 | case 'S': |
---|
956 | treenode[i]->protx[k][l][(long)serine - (long)alanine] = 1.0; |
---|
957 | break; |
---|
958 | |
---|
959 | case 'T': |
---|
960 | treenode[i]->protx[k][l][(long)threonine - (long)alanine] = 1.0; |
---|
961 | break; |
---|
962 | |
---|
963 | case 'W': |
---|
964 | treenode[i]->protx[k][l][(long)tryptophan - (long)alanine] = 1.0; |
---|
965 | break; |
---|
966 | |
---|
967 | case 'Y': |
---|
968 | treenode[i]->protx[k][l][(long)tyrosine - (long)alanine] = 1.0; |
---|
969 | break; |
---|
970 | |
---|
971 | case 'V': |
---|
972 | treenode[i]->protx[k][l][(long)valine - (long)alanine] = 1.0; |
---|
973 | break; |
---|
974 | |
---|
975 | case 'B': |
---|
976 | treenode[i]->protx[k][l][(long)asparagine - (long)alanine] = 1.0; |
---|
977 | treenode[i]->protx[k][l][(long)aspartic - (long)alanine] = 1.0; |
---|
978 | break; |
---|
979 | |
---|
980 | case 'Z': |
---|
981 | treenode[i]->protx[k][l][(long)glutamine - (long)alanine] = 1.0; |
---|
982 | treenode[i]->protx[k][l][(long)glutamic - (long)alanine] = 1.0; |
---|
983 | break; |
---|
984 | |
---|
985 | case 'X': /* unknown aa */ |
---|
986 | for (b = 0; b <= 19; b++) |
---|
987 | treenode[i]->protx[k][l][b] = 1.0; |
---|
988 | break; |
---|
989 | |
---|
990 | case '?': /* unknown aa */ |
---|
991 | for (b = 0; b <= 19; b++) |
---|
992 | treenode[i]->protx[k][l][b] = 1.0; |
---|
993 | break; |
---|
994 | |
---|
995 | case '*': /* stop codon symbol */ |
---|
996 | for (b = 0; b <= 19; b++) |
---|
997 | treenode[i]->protx[k][l][b] = 1.0; |
---|
998 | break; |
---|
999 | |
---|
1000 | case '-': /* deletion event-absent data or aa */ |
---|
1001 | for (b = 0; b <= 19; b++) |
---|
1002 | treenode[i]->protx[k][l][b] = 1.0; |
---|
1003 | break; |
---|
1004 | } |
---|
1005 | } |
---|
1006 | } |
---|
1007 | } |
---|
1008 | } /* prot_makevalues */ |
---|
1009 | |
---|
1010 | |
---|
1011 | void alloc_pmatrix(long sib) |
---|
1012 | { |
---|
1013 | /* Allocate memory for a new pmatrix. Called iff num_sibs>max_num_sibs */ |
---|
1014 | long j, k, l; |
---|
1015 | double ****temp_matrix; |
---|
1016 | |
---|
1017 | temp_matrix = (double ****) Malloc (rcategs * sizeof(double ***)); |
---|
1018 | for (j = 0; j < rcategs; j++) { |
---|
1019 | temp_matrix[j] = (double ***) Malloc(categs * sizeof(double **)); |
---|
1020 | for (k = 0; k < categs; k++) { |
---|
1021 | temp_matrix[j][k] = (double **) Malloc(20 * sizeof (double *)); |
---|
1022 | for (l = 0; l < 20; l++) |
---|
1023 | temp_matrix[j][k][l] = (double *) Malloc(20 * sizeof(double)); |
---|
1024 | } |
---|
1025 | } |
---|
1026 | pmatrices[sib] = temp_matrix; |
---|
1027 | max_num_sibs++; |
---|
1028 | } /* alloc_pmatrix */ |
---|
1029 | |
---|
1030 | |
---|
1031 | void prot_inittable() |
---|
1032 | { |
---|
1033 | /* Define a lookup table. Precompute values and print them out in tables */ |
---|
1034 | /* Allocate memory for the pmatrices, dpmatices and ddpmatrices */ |
---|
1035 | long i, j, k, l; |
---|
1036 | double sumrates; |
---|
1037 | |
---|
1038 | /* Allocate memory for pmatrices, the array of pointers to pmatrices */ |
---|
1039 | |
---|
1040 | pmatrices = (double *****) Malloc ( spp * sizeof(double ****)); |
---|
1041 | |
---|
1042 | /* Allocate memory for the first 2 pmatrices, the matrix of conversion */ |
---|
1043 | /* probabilities, but only once per run (aka not on the second jumble. */ |
---|
1044 | |
---|
1045 | alloc_pmatrix(0); |
---|
1046 | alloc_pmatrix(1); |
---|
1047 | |
---|
1048 | /* Allocate memory for one dpmatrix, the first derivative matrix */ |
---|
1049 | |
---|
1050 | dpmatrix = (double ****) Malloc( rcategs * sizeof(double ***)); |
---|
1051 | for (j = 0; j < rcategs; j++) { |
---|
1052 | dpmatrix[j] = (double ***) Malloc( categs * sizeof(double **)); |
---|
1053 | for (k = 0; k < categs; k++) { |
---|
1054 | dpmatrix[j][k] = (double **) Malloc( 20 * sizeof(double *)); |
---|
1055 | for (l = 0; l < 20; l++) |
---|
1056 | dpmatrix[j][k][l] = (double *) Malloc( 20 * sizeof(double)); |
---|
1057 | } |
---|
1058 | } |
---|
1059 | |
---|
1060 | /* Allocate memory for one ddpmatrix, the second derivative matrix */ |
---|
1061 | ddpmatrix = (double ****) Malloc( rcategs * sizeof(double ***)); |
---|
1062 | for (j = 0; j < rcategs; j++) { |
---|
1063 | ddpmatrix[j] = (double ***) Malloc( categs * sizeof(double **)); |
---|
1064 | for (k = 0; k < categs; k++) { |
---|
1065 | ddpmatrix[j][k] = (double **) Malloc( 20 * sizeof(double *)); |
---|
1066 | for (l = 0; l < 20; l++) |
---|
1067 | ddpmatrix[j][k][l] = (double *) Malloc( 20 * sizeof(double)); |
---|
1068 | } |
---|
1069 | } |
---|
1070 | |
---|
1071 | /* Allocate memory and assign values to tbl, the matrix of possible rates*/ |
---|
1072 | |
---|
1073 | tbl = (double **) Malloc( rcategs * sizeof(double *)); |
---|
1074 | for (j = 0; j < rcategs; j++) |
---|
1075 | tbl[j] = (double *) Malloc( categs * sizeof(double)); |
---|
1076 | |
---|
1077 | for (j = 0; j < rcategs; j++) |
---|
1078 | for (k = 0; k < categs; k++) |
---|
1079 | tbl[j][k] = rrate[j]*rate[k]; |
---|
1080 | |
---|
1081 | sumrates = 0.0; |
---|
1082 | for (i = 0; i < endsite; i++) { |
---|
1083 | for (j = 0; j < rcategs; j++) |
---|
1084 | sumrates += aliasweight[i] * probcat[j] |
---|
1085 | * tbl[j][category[alias[i] - 1] - 1]; |
---|
1086 | } |
---|
1087 | sumrates /= (double)sites; |
---|
1088 | for (j = 0; j < rcategs; j++) |
---|
1089 | for (k = 0; k < categs; k++) { |
---|
1090 | tbl[j][k] /= sumrates; |
---|
1091 | } |
---|
1092 | if (gama) { |
---|
1093 | fprintf(outfile, "\nDiscrete approximation to gamma distributed rates\n"); |
---|
1094 | fprintf(outfile, |
---|
1095 | " Coefficient of variation of rates = %f (alpha = %f)\n", |
---|
1096 | cv, alpha); |
---|
1097 | } |
---|
1098 | if (rcategs > 1) { |
---|
1099 | fprintf(outfile, "\nStates in HMM Rate of change Probability\n\n"); |
---|
1100 | for (i = 0; i < rcategs; i++) |
---|
1101 | if (probcat[i] < 0.0001) |
---|
1102 | fprintf(outfile, "%9ld%16.3f%20.6f\n", i+1, rrate[i], probcat[i]); |
---|
1103 | else if (probcat[i] < 0.001) |
---|
1104 | fprintf(outfile, "%9ld%16.3f%19.5f\n", i+1, rrate[i], probcat[i]); |
---|
1105 | else if (probcat[i] < 0.01) |
---|
1106 | fprintf(outfile, "%9ld%16.3f%18.4f\n", i+1, rrate[i], probcat[i]); |
---|
1107 | else |
---|
1108 | fprintf(outfile, "%9ld%16.3f%17.3f\n", i+1, rrate[i], probcat[i]); |
---|
1109 | putc('\n', outfile); |
---|
1110 | if (auto_) |
---|
1111 | fprintf(outfile, |
---|
1112 | "Expected length of a patch of sites having the same rate = %8.3f\n", |
---|
1113 | 1/lambda); |
---|
1114 | putc('\n', outfile); |
---|
1115 | } |
---|
1116 | if (categs > 1) { |
---|
1117 | fprintf(outfile, "\nSite category Rate of change\n\n"); |
---|
1118 | for (k = 0; k < categs; k++) |
---|
1119 | fprintf(outfile, "%9ld%16.3f\n", k+1, rate[k]); |
---|
1120 | } |
---|
1121 | if ((rcategs > 1) || (categs >> 1)) |
---|
1122 | fprintf(outfile, "\n\n"); |
---|
1123 | } /* prot_inittable */ |
---|
1124 | |
---|
1125 | |
---|
1126 | void getinput() |
---|
1127 | { |
---|
1128 | /* reads the input data */ |
---|
1129 | if (!justwts || firstset) |
---|
1130 | inputoptions(); |
---|
1131 | if (!justwts || firstset) |
---|
1132 | input_protdata(sites); |
---|
1133 | makeweights(); |
---|
1134 | setuptree2(curtree); |
---|
1135 | if (!usertree || reconsider) { |
---|
1136 | setuptree2(bestree); |
---|
1137 | setuptree2(priortree); |
---|
1138 | if (njumble > 1) |
---|
1139 | setuptree2(bestree2); |
---|
1140 | } |
---|
1141 | prot_allocx(nonodes2, rcategs, curtree.nodep, usertree); |
---|
1142 | if (!usertree || reconsider) { |
---|
1143 | prot_allocx(nonodes2, rcategs, bestree.nodep, 0); |
---|
1144 | prot_allocx(nonodes2, rcategs, priortree.nodep, 0); |
---|
1145 | if (njumble > 1) |
---|
1146 | prot_allocx(nonodes2, rcategs, bestree2.nodep, 0); |
---|
1147 | } |
---|
1148 | prot_makevalues(rcategs, curtree.nodep, endsite, spp, y, alias); |
---|
1149 | } /* getinput */ |
---|
1150 | |
---|
1151 | |
---|
1152 | void inittravtree(node *p) |
---|
1153 | { |
---|
1154 | /* traverse tree to set initialized and v to initial values */ |
---|
1155 | |
---|
1156 | p->initialized = false; |
---|
1157 | p->back->initialized = false; |
---|
1158 | if (!p->tip) { |
---|
1159 | inittravtree(p->next->back); |
---|
1160 | inittravtree(p->next->next->back); |
---|
1161 | } |
---|
1162 | } /* inittravtree */ |
---|
1163 | |
---|
1164 | |
---|
1165 | void prot_nuview(node *p) |
---|
1166 | { |
---|
1167 | long i, j, k, l, m, num_sibs, sib_index; |
---|
1168 | node *sib_ptr, *sib_back_ptr; |
---|
1169 | psitelike prot_xx, x2; |
---|
1170 | double lw, prod7; |
---|
1171 | double **pmat; |
---|
1172 | |
---|
1173 | /* Figure out how many siblings the current node has */ |
---|
1174 | /* and be sure that pmatrices is large enough */ |
---|
1175 | num_sibs = count_sibs(p); |
---|
1176 | for (i = 0; i < num_sibs; i++) |
---|
1177 | if (pmatrices[i] == NULL) |
---|
1178 | alloc_pmatrix(i); |
---|
1179 | |
---|
1180 | /* Recursive calls, should be called for all children */ |
---|
1181 | sib_ptr = p; |
---|
1182 | for (i=0 ; i < num_sibs; i++) { |
---|
1183 | sib_ptr = sib_ptr->next; |
---|
1184 | sib_back_ptr = sib_ptr->back; |
---|
1185 | |
---|
1186 | if (!sib_back_ptr->tip && |
---|
1187 | !sib_back_ptr->initialized) |
---|
1188 | prot_nuview(sib_back_ptr); |
---|
1189 | } |
---|
1190 | |
---|
1191 | /* Make pmatrices for all possible combinations of category, rcateg */ |
---|
1192 | /* and sib */ |
---|
1193 | sib_ptr = p; /* return to p */ |
---|
1194 | for (sib_index=0; sib_index < num_sibs; sib_index++) { |
---|
1195 | sib_ptr = sib_ptr->next; |
---|
1196 | sib_back_ptr = sib_ptr->back; |
---|
1197 | |
---|
1198 | lw = sib_back_ptr->v; |
---|
1199 | |
---|
1200 | for (j = 0; j < rcategs; j++) |
---|
1201 | for (k = 0; k < categs; k++) |
---|
1202 | make_pmatrix(pmatrices[sib_index][j][k], NULL, NULL, 0, lw, |
---|
1203 | tbl[j][k], eigmat, probmat); |
---|
1204 | } |
---|
1205 | |
---|
1206 | for (i = 0; i < endsite; i++) { |
---|
1207 | k = category[alias[i]-1] - 1; |
---|
1208 | for (j = 0; j < rcategs; j++) { |
---|
1209 | |
---|
1210 | /* initialize to 1 all values of prot_xx */ |
---|
1211 | for (m = 0; m <= 19; m++) |
---|
1212 | prot_xx[m] = 1; |
---|
1213 | |
---|
1214 | sib_ptr = p; /* return to p */ |
---|
1215 | /* loop through all sibs and calculate likelihoods for all possible*/ |
---|
1216 | /* amino acid combinations */ |
---|
1217 | for (sib_index=0; sib_index < num_sibs; sib_index++) { |
---|
1218 | sib_ptr = sib_ptr->next; |
---|
1219 | sib_back_ptr = sib_ptr->back; |
---|
1220 | |
---|
1221 | memcpy(x2, sib_back_ptr->protx[i][j], sizeof(psitelike)); |
---|
1222 | pmat = pmatrices[sib_index][j][k]; |
---|
1223 | for (m = 0; m <= 19; m++) { |
---|
1224 | prod7 = 0; |
---|
1225 | for (l = 0; l <= 19; l++) |
---|
1226 | prod7 += (pmat[m][l] * x2[l]); |
---|
1227 | prot_xx[m] *= prod7; |
---|
1228 | } |
---|
1229 | } |
---|
1230 | /* And the final point of this whole function: */ |
---|
1231 | memcpy(p->protx[i][j], prot_xx, sizeof(psitelike)); |
---|
1232 | } |
---|
1233 | } |
---|
1234 | |
---|
1235 | p->initialized = true; |
---|
1236 | } /* prot_nuview */ |
---|
1237 | |
---|
1238 | |
---|
1239 | void prot_slopecurv(node *p,double local_y,double *like,double *slope,double *curve) |
---|
1240 | { |
---|
1241 | /* compute log likelihood, slope and curvature at node p */ |
---|
1242 | long i, j, k, l, m, lai; |
---|
1243 | double sum, sumc, sumterm, lterm, sumcs, sumcc, sum2, slope2, curve2; |
---|
1244 | double frexm = 0; /* frexm = freqaa[m]*x1[m] */ |
---|
1245 | /* frexml = frexm*x2[l] */ |
---|
1246 | double prod4m, prod5m, prod6m; /* elements of prod4-5 for */ |
---|
1247 | /* each m */ |
---|
1248 | double **pmat, **dpmat, **ddpmat; /* local pointers to global*/ |
---|
1249 | /* matrices */ |
---|
1250 | double prod4, prod5, prod6; |
---|
1251 | contribarr thelike, nulike, nuslope, nucurve, |
---|
1252 | theslope, thecurve, clai, cslai, cclai; |
---|
1253 | node *q; |
---|
1254 | psitelike x1, x2; |
---|
1255 | |
---|
1256 | q = p->back; |
---|
1257 | sum = 0.0; |
---|
1258 | for (j = 0; j < rcategs; j++) { |
---|
1259 | for (k = 0; k < categs; k++) { |
---|
1260 | make_pmatrix(pmatrices[0][j][k], dpmatrix[j][k], ddpmatrix[j][k], |
---|
1261 | 2, local_y, tbl[j][k], eigmat, probmat); |
---|
1262 | } |
---|
1263 | } |
---|
1264 | for (i = 0; i < endsite; i++) { |
---|
1265 | k = category[alias[i]-1] - 1; |
---|
1266 | for (j = 0; j < rcategs; j++) { |
---|
1267 | memcpy(x1, p->protx[i][j], sizeof(psitelike)); |
---|
1268 | memcpy(x2, q->protx[i][j], sizeof(psitelike)); |
---|
1269 | pmat = pmatrices[0][j][k]; |
---|
1270 | dpmat = dpmatrix[j][k]; |
---|
1271 | ddpmat = ddpmatrix[j][k]; |
---|
1272 | prod4 = 0.0; |
---|
1273 | prod5 = 0.0; |
---|
1274 | prod6 = 0.0; |
---|
1275 | for (m = 0; m <= 19; m++) { |
---|
1276 | prod4m = 0.0; |
---|
1277 | prod5m = 0.0; |
---|
1278 | prod6m = 0.0; |
---|
1279 | frexm = x1[m] * freqaa[m]; |
---|
1280 | for (l = 0; l <= 19; l++) { |
---|
1281 | prod4m += x2[l] * pmat[m][l]; |
---|
1282 | prod5m += x2[l] * dpmat[m][l]; |
---|
1283 | prod6m += x2[l] * ddpmat[m][l]; |
---|
1284 | } |
---|
1285 | prod4 += frexm * prod4m; |
---|
1286 | prod5 += frexm * prod5m; |
---|
1287 | prod6 += frexm * prod6m; |
---|
1288 | } |
---|
1289 | term[i][j] = prod4; |
---|
1290 | slopeterm[i][j] = prod5; |
---|
1291 | curveterm[i][j] = prod6; |
---|
1292 | } |
---|
1293 | sumterm = 0.0; |
---|
1294 | for (j = 0; j < rcategs; j++) |
---|
1295 | sumterm += probcat[j] * term[i][j]; |
---|
1296 | if (sumterm < 0.0) |
---|
1297 | sumterm = 0.00000001; /* ??? */ |
---|
1298 | lterm = log(sumterm); |
---|
1299 | for (j = 0; j < rcategs; j++) { |
---|
1300 | term[i][j] = term[i][j] / sumterm; |
---|
1301 | slopeterm[i][j] = slopeterm[i][j] / sumterm; |
---|
1302 | curveterm[i][j] = curveterm[i][j] / sumterm; |
---|
1303 | } |
---|
1304 | sum += (aliasweight[i] * lterm); |
---|
1305 | } |
---|
1306 | for (i = 0; i < rcategs; i++) { |
---|
1307 | thelike[i] = 1.0; |
---|
1308 | theslope[i] = 0.0; |
---|
1309 | thecurve[i] = 0.0; |
---|
1310 | } |
---|
1311 | for (i = 0; i < sites; i++) { |
---|
1312 | sumc = 0.0; |
---|
1313 | sumcs = 0.0; |
---|
1314 | sumcc = 0.0; |
---|
1315 | for (k = 0; k < rcategs; k++) { |
---|
1316 | sumc += probcat[k] * thelike[k]; |
---|
1317 | sumcs += probcat[k] * theslope[k]; |
---|
1318 | sumcc += probcat[k] * thecurve[k]; |
---|
1319 | } |
---|
1320 | sumc *= lambda; |
---|
1321 | sumcs *= lambda; |
---|
1322 | sumcc *= lambda; |
---|
1323 | if ((ally[i] > 0) && (location[ally[i]-1] > 0)) { |
---|
1324 | lai = location[ally[i] - 1]; |
---|
1325 | memcpy(clai, term[lai - 1], rcategs*sizeof(double)); |
---|
1326 | memcpy(cslai, slopeterm[lai - 1], rcategs*sizeof(double)); |
---|
1327 | memcpy(cclai, curveterm[lai - 1], rcategs*sizeof(double)); |
---|
1328 | if (weight[i] > 1) { |
---|
1329 | for (j = 0; j < rcategs; j++) { |
---|
1330 | if (clai[j] > 0.0) |
---|
1331 | clai[j] = exp(weight[i]*log(clai[j])); |
---|
1332 | else clai[j] = 0.0; |
---|
1333 | if (cslai[j] > 0.0) |
---|
1334 | cslai[j] = exp(weight[i]*log(cslai[j])); |
---|
1335 | else cslai[j] = 0.0; |
---|
1336 | if (cclai[j] > 0.0) |
---|
1337 | cclai[j] = exp(weight[i]*log(cclai[j])); |
---|
1338 | else cclai[j] = 0.0; |
---|
1339 | } |
---|
1340 | } |
---|
1341 | for (j = 0; j < rcategs; j++) { |
---|
1342 | nulike[j] = ((1.0 - lambda) * thelike[j] + sumc) * clai[j]; |
---|
1343 | nuslope[j] = ((1.0 - lambda) * theslope[j] + sumcs) * clai[j] |
---|
1344 | + ((1.0 - lambda) * thelike[j] + sumc) * cslai[j]; |
---|
1345 | nucurve[j] = ((1.0 - lambda) * thecurve[j] + sumcc) * clai[j] |
---|
1346 | + 2.0 * ((1.0 - lambda) * theslope[j] + sumcs) * cslai[j] |
---|
1347 | + ((1.0 - lambda) * thelike[j] + sumc) * cclai[j]; |
---|
1348 | } |
---|
1349 | } else { |
---|
1350 | for (j = 0; j < rcategs; j++) { |
---|
1351 | nulike[j] = ((1.0 - lambda) * thelike[j] + sumc); |
---|
1352 | nuslope[j] = ((1.0 - lambda) * theslope[j] + sumcs); |
---|
1353 | nucurve[j] = ((1.0 - lambda) * thecurve[j] + sumcc); |
---|
1354 | } |
---|
1355 | } |
---|
1356 | memcpy(thelike, nulike, rcategs*sizeof(double)); |
---|
1357 | memcpy(theslope, nuslope, rcategs*sizeof(double)); |
---|
1358 | memcpy(thecurve, nucurve, rcategs*sizeof(double)); |
---|
1359 | } |
---|
1360 | sum2 = 0.0; |
---|
1361 | slope2 = 0.0; |
---|
1362 | curve2 = 0.0; |
---|
1363 | for (i = 0; i < rcategs; i++) { |
---|
1364 | sum2 += probcat[i] * thelike[i]; |
---|
1365 | slope2 += probcat[i] * theslope[i]; |
---|
1366 | curve2 += probcat[i] * thecurve[i]; |
---|
1367 | } |
---|
1368 | sum += log(sum2); |
---|
1369 | (*like) = sum; |
---|
1370 | (*slope) = slope2 / sum2; |
---|
1371 | (*curve) = (curve2 - slope2 * slope2 / sum2) / sum2; |
---|
1372 | } /* prot_slopecurv */ |
---|
1373 | |
---|
1374 | |
---|
1375 | void makenewv(node *p) |
---|
1376 | { |
---|
1377 | /* Newton-Raphson algorithm improvement of a branch length */ |
---|
1378 | long it, ite; |
---|
1379 | double local_y, yold=0, yorig, like, slope, curve, oldlike=0; |
---|
1380 | boolean done, firsttime, better; |
---|
1381 | node *q; |
---|
1382 | |
---|
1383 | q = p->back; |
---|
1384 | local_y = p->v; |
---|
1385 | yorig = local_y; |
---|
1386 | done = false; |
---|
1387 | firsttime = true; |
---|
1388 | it = 1; |
---|
1389 | ite = 0; |
---|
1390 | while ((it < iterations) && (ite < 20) && (!done)) { |
---|
1391 | prot_slopecurv(p, local_y, &like, &slope, &curve); |
---|
1392 | better = false; |
---|
1393 | if (firsttime) { |
---|
1394 | yold = local_y; |
---|
1395 | oldlike = like; |
---|
1396 | firsttime = false; |
---|
1397 | better = true; |
---|
1398 | } else { |
---|
1399 | if (like > oldlike) { |
---|
1400 | yold = local_y; |
---|
1401 | oldlike = like; |
---|
1402 | better = true; |
---|
1403 | it++; |
---|
1404 | } |
---|
1405 | } |
---|
1406 | if (better) { |
---|
1407 | local_y = local_y + slope/fabs(curve); |
---|
1408 | if (local_y < epsilon) |
---|
1409 | local_y = epsilon; |
---|
1410 | } else { |
---|
1411 | if (fabs(local_y - yold) < epsilon) |
---|
1412 | ite = 20; |
---|
1413 | local_y = (local_y + (7 * yold)) / 8; |
---|
1414 | } |
---|
1415 | ite++; |
---|
1416 | done = fabs(local_y-yold) < epsilon; |
---|
1417 | } |
---|
1418 | smoothed = (fabs(yold-yorig) < epsilon) && (yorig > 1000.0*epsilon); |
---|
1419 | p->v = yold; |
---|
1420 | q->v = yold; |
---|
1421 | curtree.likelihood = oldlike; |
---|
1422 | } /* makenewv */ |
---|
1423 | |
---|
1424 | |
---|
1425 | void update(node *p) |
---|
1426 | { |
---|
1427 | long num_sibs, i; |
---|
1428 | node *sib_ptr; |
---|
1429 | |
---|
1430 | if (!p->tip && !p->initialized) |
---|
1431 | prot_nuview(p); |
---|
1432 | if (!p->back->tip && !p->back->initialized) |
---|
1433 | prot_nuview(p->back); |
---|
1434 | if (p->iter) { |
---|
1435 | makenewv(p); |
---|
1436 | |
---|
1437 | if (!p->tip) { |
---|
1438 | num_sibs = count_sibs(p); |
---|
1439 | sib_ptr = p; |
---|
1440 | for (i=0; i < num_sibs; i++) { |
---|
1441 | sib_ptr = sib_ptr->next; |
---|
1442 | sib_ptr->initialized = false; |
---|
1443 | } |
---|
1444 | } |
---|
1445 | |
---|
1446 | if (!p->back->tip) { |
---|
1447 | num_sibs = count_sibs(p->back); |
---|
1448 | sib_ptr = p->back; |
---|
1449 | for (i=0; i < num_sibs; i++) { |
---|
1450 | sib_ptr = sib_ptr->next; |
---|
1451 | sib_ptr->initialized = false; |
---|
1452 | } |
---|
1453 | } |
---|
1454 | } |
---|
1455 | } /* update */ |
---|
1456 | |
---|
1457 | |
---|
1458 | void smooth(node *p) |
---|
1459 | { |
---|
1460 | long i, num_sibs; |
---|
1461 | node *sib_ptr; |
---|
1462 | |
---|
1463 | smoothed = false; |
---|
1464 | update(p); |
---|
1465 | if (p->tip) |
---|
1466 | return; |
---|
1467 | |
---|
1468 | num_sibs = count_sibs(p); |
---|
1469 | sib_ptr = p; |
---|
1470 | |
---|
1471 | for (i=0; i < num_sibs; i++) { |
---|
1472 | sib_ptr = sib_ptr->next; |
---|
1473 | |
---|
1474 | if (polishing || (smoothit && !smoothed)) { |
---|
1475 | smooth(sib_ptr->back); |
---|
1476 | p->initialized = false; |
---|
1477 | sib_ptr->initialized = false; |
---|
1478 | } |
---|
1479 | } |
---|
1480 | } /* smooth */ |
---|
1481 | |
---|
1482 | |
---|
1483 | void make_pmatrix(double **matrix, double **dmat, double **ddmat, |
---|
1484 | long derivative, double lz, double rat, |
---|
1485 | double *local_eigmat, double **local_probmat) |
---|
1486 | { |
---|
1487 | /* Computes the R matrix such that matrix[m][l] is the joint probability */ |
---|
1488 | /* of m and l. */ |
---|
1489 | /* Computes a P matrix such that matrix[m][l] is the conditional */ |
---|
1490 | /* probability of m given l. This is accomplished by dividing all terms */ |
---|
1491 | /* in the R matrix by freqaa[m], the frequency of l. */ |
---|
1492 | |
---|
1493 | long k, l, m; /* (l) original character state */ |
---|
1494 | /* (m) final character state */ |
---|
1495 | /* (k) lambda counter */ |
---|
1496 | double p0, p1, p2, q; |
---|
1497 | double elambdat[20], delambdat[20], ddelambdat[20]; |
---|
1498 | /* exponential term for matrix */ |
---|
1499 | /* and both derivative matrices */ |
---|
1500 | |
---|
1501 | for (k = 0; k <= 19; k++) { |
---|
1502 | elambdat[k] = exp(lz * rat * local_eigmat[k]); |
---|
1503 | if(derivative != 0) { |
---|
1504 | delambdat[k] = (elambdat[k] * rat * local_eigmat[k]); |
---|
1505 | ddelambdat[k] = (delambdat[k] * rat * local_eigmat[k]); |
---|
1506 | } |
---|
1507 | } |
---|
1508 | for (m = 0; m <= 19; m++) { |
---|
1509 | for (l = 0; l <= 19; l++) { |
---|
1510 | p0 = 0.0; |
---|
1511 | p1 = 0.0; |
---|
1512 | p2 = 0.0; |
---|
1513 | for (k = 0; k <= 19; k++) { |
---|
1514 | q = local_probmat[k][m] * local_probmat[k][l]; |
---|
1515 | p0 += (q * elambdat[k]); |
---|
1516 | if(derivative !=0) { |
---|
1517 | p1 += (q * delambdat[k]); |
---|
1518 | p2 += (q * ddelambdat[k]); |
---|
1519 | } |
---|
1520 | } |
---|
1521 | matrix[m][l] = p0 / freqaa[m]; |
---|
1522 | if(derivative != 0) { |
---|
1523 | dmat[m][l] = p1 / freqaa[m]; |
---|
1524 | ddmat[m][l] = p2 / freqaa[m]; |
---|
1525 | } |
---|
1526 | } |
---|
1527 | } |
---|
1528 | } /* make_pmatrix */ |
---|
1529 | |
---|
1530 | |
---|
1531 | double prot_evaluate(node *p, boolean saveit) |
---|
1532 | { |
---|
1533 | contribarr tterm; |
---|
1534 | double sum, sum2, sumc, local_y, prod4, prodl, frexm, sumterm, lterm; |
---|
1535 | double **pmat; |
---|
1536 | long i, j, k, l, m, lai; |
---|
1537 | node *q; |
---|
1538 | psitelike x1, x2; |
---|
1539 | |
---|
1540 | sum = 0.0; |
---|
1541 | q = p->back; |
---|
1542 | local_y = p->v; |
---|
1543 | for (j = 0; j < rcategs; j++) |
---|
1544 | for (k = 0; k < categs; k++) |
---|
1545 | make_pmatrix(pmatrices[0][j][k],NULL,NULL,0,local_y,tbl[j][k],eigmat,probmat); |
---|
1546 | for (i = 0; i < endsite; i++) { |
---|
1547 | k = category[alias[i]-1] - 1; |
---|
1548 | for (j = 0; j < rcategs; j++) { |
---|
1549 | memcpy(x1, p->protx[i][j], sizeof(psitelike)); |
---|
1550 | memcpy(x2, q->protx[i][j], sizeof(psitelike)); |
---|
1551 | prod4 = 0.0; |
---|
1552 | pmat = pmatrices[0][j][k]; |
---|
1553 | for (m = 0; m <= 19; m++) { |
---|
1554 | prodl = 0.0; |
---|
1555 | for (l = 0; l <= 19; l++) |
---|
1556 | prodl += (pmat[m][l] * x2[l]); |
---|
1557 | frexm = x1[m] * freqaa[m]; |
---|
1558 | prod4 += (prodl * frexm); |
---|
1559 | } |
---|
1560 | tterm[j] = prod4; |
---|
1561 | } |
---|
1562 | sumterm = 0.0; |
---|
1563 | for (j = 0; j < rcategs; j++) |
---|
1564 | sumterm += probcat[j] * tterm[j]; |
---|
1565 | if (sumterm < 0.0) |
---|
1566 | sumterm = 0.00000001; /* ??? */ |
---|
1567 | lterm = log(sumterm); |
---|
1568 | for (j = 0; j < rcategs; j++) |
---|
1569 | global_clai[j] = tterm[j] / sumterm; |
---|
1570 | memcpy(contribution[i], global_clai, rcategs*sizeof(double)); |
---|
1571 | if (saveit && !auto_ && usertree) |
---|
1572 | l0gf[which - 1][i] = lterm; |
---|
1573 | sum += aliasweight[i] * lterm; |
---|
1574 | } |
---|
1575 | for (j = 0; j < rcategs; j++) |
---|
1576 | global_like[j] = 1.0; |
---|
1577 | for (i = 0; i < sites; i++) { |
---|
1578 | sumc = 0.0; |
---|
1579 | for (k = 0; k < rcategs; k++) |
---|
1580 | sumc += probcat[k] * global_like[k]; |
---|
1581 | sumc *= lambda; |
---|
1582 | if ((ally[i] > 0) && (location[ally[i]-1] > 0)) { |
---|
1583 | lai = location[ally[i] - 1]; |
---|
1584 | memcpy(global_clai, contribution[lai - 1], rcategs*sizeof(double)); |
---|
1585 | for (j = 0; j < rcategs; j++) |
---|
1586 | global_nulike[j] = ((1.0 - lambda) * global_like[j] + sumc) * global_clai[j]; |
---|
1587 | } else { |
---|
1588 | for (j = 0; j < rcategs; j++) |
---|
1589 | global_nulike[j] = ((1.0 - lambda) * global_like[j] + sumc); |
---|
1590 | } |
---|
1591 | memcpy(global_like, global_nulike, rcategs*sizeof(double)); |
---|
1592 | } |
---|
1593 | sum2 = 0.0; |
---|
1594 | for (i = 0; i < rcategs; i++) |
---|
1595 | sum2 += probcat[i] * global_like[i]; |
---|
1596 | sum += log(sum2); |
---|
1597 | curtree.likelihood = sum; |
---|
1598 | if (!saveit || auto_ || !usertree) |
---|
1599 | return sum; |
---|
1600 | l0gl[which - 1] = sum; |
---|
1601 | if (which == 1) { |
---|
1602 | maxwhich = 1; |
---|
1603 | maxlogl = sum; |
---|
1604 | return sum; |
---|
1605 | } |
---|
1606 | if (sum > maxlogl) { |
---|
1607 | maxwhich = which; |
---|
1608 | maxlogl = sum; |
---|
1609 | } |
---|
1610 | return sum; |
---|
1611 | } /* prot_evaluate */ |
---|
1612 | |
---|
1613 | |
---|
1614 | void treevaluate() |
---|
1615 | { |
---|
1616 | /* evaluate a user tree */ |
---|
1617 | long i; |
---|
1618 | |
---|
1619 | inittravtree(curtree.start); |
---|
1620 | polishing = true; |
---|
1621 | smoothit = true; |
---|
1622 | for (i = 1; i <= smoothings * 4; i++) |
---|
1623 | smooth (curtree.start); |
---|
1624 | dummy = prot_evaluate(curtree.start, true); |
---|
1625 | } /* treevaluate */ |
---|
1626 | |
---|
1627 | |
---|
1628 | void promlcopy(tree *a, tree *b, long local_nonodes, long local_categs) |
---|
1629 | { |
---|
1630 | /* used in proml */ |
---|
1631 | long i, j=0; |
---|
1632 | node *p, *q; |
---|
1633 | |
---|
1634 | for (i = 0; i < spp; i++) { |
---|
1635 | prot_copynode(a->nodep[i], b->nodep[i], local_categs); |
---|
1636 | if (a->nodep[i]->back) { |
---|
1637 | if (a->nodep[i]->back == a->nodep[a->nodep[i]->back->index - 1]) |
---|
1638 | b->nodep[i]->back = b->nodep[a->nodep[i]->back->index - 1]; |
---|
1639 | else if (a->nodep[i]->back == a->nodep[a->nodep[i]->back->index - 1]->next |
---|
1640 | ) |
---|
1641 | b->nodep[i]->back = b->nodep[a->nodep[i]->back->index - 1]->next; |
---|
1642 | else |
---|
1643 | b->nodep[i]->back = b->nodep[a->nodep[i]->back->index - 1]->next->next; |
---|
1644 | } |
---|
1645 | else b->nodep[i]->back = NULL; |
---|
1646 | } |
---|
1647 | for (i = spp; i < local_nonodes; i++) { |
---|
1648 | p = a->nodep[i]; |
---|
1649 | q = b->nodep[i]; |
---|
1650 | for (j = 1; j <= 3; j++) { |
---|
1651 | prot_copynode(p, q, local_categs); |
---|
1652 | if (p->back) { |
---|
1653 | if (p->back == a->nodep[p->back->index - 1]) |
---|
1654 | q->back = b->nodep[p->back->index - 1]; |
---|
1655 | else if (p->back == a->nodep[p->back->index - 1]->next) |
---|
1656 | q->back = b->nodep[p->back->index - 1]->next; |
---|
1657 | else |
---|
1658 | q->back = b->nodep[p->back->index - 1]->next->next; |
---|
1659 | } |
---|
1660 | else |
---|
1661 | q->back = NULL; |
---|
1662 | p = p->next; |
---|
1663 | q = q->next; |
---|
1664 | } |
---|
1665 | } |
---|
1666 | b->likelihood = a->likelihood; |
---|
1667 | b->start = a->start; /* start used in dnaml only */ |
---|
1668 | b->root = a->root; /* root used in dnamlk only */ |
---|
1669 | } /* promlcopy */ |
---|
1670 | |
---|
1671 | |
---|
1672 | void proml_re_move(node **p, node **q) |
---|
1673 | { |
---|
1674 | /* remove p and record in q where it was */ |
---|
1675 | long i; |
---|
1676 | |
---|
1677 | /** assumes bifurcations */ |
---|
1678 | *q = (*p)->next->back; |
---|
1679 | hookup(*q, (*p)->next->next->back); |
---|
1680 | (*p)->next->back = NULL; |
---|
1681 | (*p)->next->next->back = NULL; |
---|
1682 | inittrav((*q)); |
---|
1683 | inittrav((*q)->back); |
---|
1684 | i = 1; |
---|
1685 | while (i <= smoothings) { |
---|
1686 | smooth(*q); |
---|
1687 | if (smoothit) |
---|
1688 | smooth((*q)->back); |
---|
1689 | i++; |
---|
1690 | } |
---|
1691 | } /* proml_re_move */ |
---|
1692 | |
---|
1693 | |
---|
1694 | void insert_(node *p, node *q, boolean dooinit) |
---|
1695 | { |
---|
1696 | /* Insert q near p */ |
---|
1697 | long i, j, num_sibs; |
---|
1698 | node *r, *sib_ptr; |
---|
1699 | |
---|
1700 | r = p->next->next; |
---|
1701 | hookup(r, q->back); |
---|
1702 | hookup(p->next, q); |
---|
1703 | q->v = 0.5 * q->v; |
---|
1704 | q->back->v = q->v; |
---|
1705 | r->v = q->v; |
---|
1706 | r->back->v = r->v; |
---|
1707 | p->initialized = false; |
---|
1708 | p->next->initialized = false; |
---|
1709 | p->next->next->initialized = false; |
---|
1710 | if (dooinit) { |
---|
1711 | inittrav(p); |
---|
1712 | inittrav(q); |
---|
1713 | inittrav(q->back); |
---|
1714 | } |
---|
1715 | i = 1; |
---|
1716 | while (i <= smoothings) { |
---|
1717 | smooth(p); |
---|
1718 | if (!smoothit) { |
---|
1719 | if (!p->tip) { |
---|
1720 | num_sibs = count_sibs(p); |
---|
1721 | sib_ptr = p; |
---|
1722 | for (j=0; j < num_sibs; j++) { |
---|
1723 | smooth(sib_ptr->next->back); |
---|
1724 | sib_ptr = sib_ptr->next; |
---|
1725 | } |
---|
1726 | } |
---|
1727 | } |
---|
1728 | else |
---|
1729 | smooth(p->back); |
---|
1730 | i++; |
---|
1731 | } |
---|
1732 | } /* insert_ */ |
---|
1733 | |
---|
1734 | |
---|
1735 | void addtraverse(node *p, node *q, boolean contin) |
---|
1736 | { |
---|
1737 | /* try adding p at q, proceed recursively through tree */ |
---|
1738 | long i, num_sibs; |
---|
1739 | double like, vsave = 0; |
---|
1740 | node *qback = NULL, *sib_ptr; |
---|
1741 | |
---|
1742 | if (!smoothit) { |
---|
1743 | vsave = q->v; |
---|
1744 | qback = q->back; |
---|
1745 | } |
---|
1746 | insert_(p, q, false); |
---|
1747 | like = prot_evaluate(p, false); |
---|
1748 | if (like > bestyet || bestyet == UNDEFINED) { |
---|
1749 | bestyet = like; |
---|
1750 | if (smoothit) |
---|
1751 | promlcopy(&curtree, &bestree, nonodes2, rcategs); |
---|
1752 | else |
---|
1753 | qwhere = q; |
---|
1754 | succeeded = true; |
---|
1755 | } |
---|
1756 | if (smoothit) |
---|
1757 | promlcopy(&priortree, &curtree, nonodes2, rcategs); |
---|
1758 | else { |
---|
1759 | hookup (q, qback); |
---|
1760 | q->v = vsave; |
---|
1761 | q->back->v = vsave; |
---|
1762 | curtree.likelihood = bestyet; |
---|
1763 | } |
---|
1764 | if (!q->tip && contin) { |
---|
1765 | num_sibs = count_sibs(q); |
---|
1766 | if (q == curtree.start) |
---|
1767 | num_sibs++; |
---|
1768 | sib_ptr = q; |
---|
1769 | for (i=0; i < num_sibs; i++) { |
---|
1770 | addtraverse(p, sib_ptr->next->back, contin); |
---|
1771 | sib_ptr = sib_ptr->next; |
---|
1772 | } |
---|
1773 | } |
---|
1774 | } /* addtraverse */ |
---|
1775 | |
---|
1776 | |
---|
1777 | void rearrange(node *p, node *pp) |
---|
1778 | { |
---|
1779 | /* rearranges the tree, globally or locally moving pp around near p */ |
---|
1780 | long i, num_sibs; |
---|
1781 | double v3 = 0, v4 = 0, v5 = 0; |
---|
1782 | node *q, *r, *sib_ptr; |
---|
1783 | |
---|
1784 | if (!p->tip && !p->back->tip) { |
---|
1785 | curtree.likelihood = bestyet; |
---|
1786 | if (p->back->next != pp) |
---|
1787 | r = p->back->next; |
---|
1788 | else |
---|
1789 | r = p->back->next->next; |
---|
1790 | /* assumes bifurcations? */ |
---|
1791 | if (!smoothit) { |
---|
1792 | v3 = r->v; |
---|
1793 | v4 = r->next->v; |
---|
1794 | v5 = r->next->next->v; |
---|
1795 | } |
---|
1796 | else |
---|
1797 | promlcopy(&curtree, &bestree, nonodes2, rcategs); |
---|
1798 | proml_re_move(&r, &q); |
---|
1799 | if (smoothit) |
---|
1800 | promlcopy(&curtree, &priortree, nonodes2, rcategs); |
---|
1801 | else |
---|
1802 | qwhere = q; |
---|
1803 | num_sibs = count_sibs (p); |
---|
1804 | sib_ptr = p; |
---|
1805 | for (i=0; i < num_sibs; i++) { |
---|
1806 | sib_ptr = sib_ptr->next; |
---|
1807 | addtraverse(r, sib_ptr->back, (boolean)(global && (nextsp == spp))); |
---|
1808 | } |
---|
1809 | if (global && nextsp == spp && !succeeded) { |
---|
1810 | p = p->back; |
---|
1811 | if (!p->tip) { |
---|
1812 | num_sibs = count_sibs (p); |
---|
1813 | sib_ptr = p; |
---|
1814 | for (i=0; i < num_sibs; i++) { |
---|
1815 | sib_ptr = sib_ptr->next; |
---|
1816 | addtraverse(r, sib_ptr->back, (boolean)(global && (nextsp == spp))); |
---|
1817 | } |
---|
1818 | } |
---|
1819 | p = p->back; |
---|
1820 | } |
---|
1821 | if (smoothit) |
---|
1822 | promlcopy(&bestree, &curtree, nonodes2, rcategs); |
---|
1823 | else { |
---|
1824 | insert_(r, qwhere, true); |
---|
1825 | if (qwhere == q) { |
---|
1826 | r->v = v3; |
---|
1827 | r->back->v = v3; |
---|
1828 | r->next->v = v4; |
---|
1829 | r->next->back->v = v4; |
---|
1830 | r->next->next->v = v5; |
---|
1831 | r->next->next->back->v = v5; |
---|
1832 | curtree.likelihood = bestyet; |
---|
1833 | } |
---|
1834 | else { |
---|
1835 | smoothit = true; |
---|
1836 | for (i = 1; i<=smoothings; i++) { |
---|
1837 | smooth(r); |
---|
1838 | smooth(r->back); |
---|
1839 | } |
---|
1840 | smoothit = false; |
---|
1841 | promlcopy(&curtree, &bestree, nonodes2, rcategs); |
---|
1842 | } |
---|
1843 | } |
---|
1844 | if (global && nextsp == spp && progress) { |
---|
1845 | putchar('.'); |
---|
1846 | fflush(stdout); |
---|
1847 | } |
---|
1848 | } |
---|
1849 | if (!p->tip) { |
---|
1850 | num_sibs = count_sibs(p); |
---|
1851 | if (p == curtree.start) |
---|
1852 | num_sibs++; |
---|
1853 | sib_ptr = p; |
---|
1854 | for (i=0; i < num_sibs; i++) { |
---|
1855 | sib_ptr = sib_ptr->next; |
---|
1856 | rearrange(sib_ptr->back, p); |
---|
1857 | } |
---|
1858 | } |
---|
1859 | } /* rearrange */ |
---|
1860 | |
---|
1861 | |
---|
1862 | void proml_coordinates(node *p, double lengthsum, long *tipy, |
---|
1863 | double *tipmax) |
---|
1864 | { |
---|
1865 | /* establishes coordinates of nodes */ |
---|
1866 | node *q, *first, *last; |
---|
1867 | double xx; |
---|
1868 | |
---|
1869 | if (p->tip) { |
---|
1870 | p->xcoord = (long)(over * lengthsum + 0.5); |
---|
1871 | p->ycoord = (*tipy); |
---|
1872 | p->ymin = (*tipy); |
---|
1873 | p->ymax = (*tipy); |
---|
1874 | (*tipy) += down; |
---|
1875 | if (lengthsum > (*tipmax)) |
---|
1876 | (*tipmax) = lengthsum; |
---|
1877 | return; |
---|
1878 | } |
---|
1879 | q = p->next; |
---|
1880 | do { |
---|
1881 | xx = q->v; |
---|
1882 | if (xx > 100.0) |
---|
1883 | xx = 100.0; |
---|
1884 | proml_coordinates(q->back, lengthsum + xx, tipy,tipmax); |
---|
1885 | q = q->next; |
---|
1886 | } while ((p == curtree.start || p != q) && |
---|
1887 | (p != curtree.start || p->next != q)); |
---|
1888 | first = p->next->back; |
---|
1889 | q = p; |
---|
1890 | while (q->next != p) |
---|
1891 | q = q->next; |
---|
1892 | last = q->back; |
---|
1893 | p->xcoord = (long)(over * lengthsum + 0.5); |
---|
1894 | if (p == curtree.start) |
---|
1895 | p->ycoord = p->next->next->back->ycoord; |
---|
1896 | else |
---|
1897 | p->ycoord = (first->ycoord + last->ycoord) / 2; |
---|
1898 | p->ymin = first->ymin; |
---|
1899 | p->ymax = last->ymax; |
---|
1900 | } /* proml_coordinates */ |
---|
1901 | |
---|
1902 | |
---|
1903 | void proml_printree() |
---|
1904 | { |
---|
1905 | /* prints out diagram of the tree2 */ |
---|
1906 | long tipy; |
---|
1907 | double scale, tipmax; |
---|
1908 | long i; |
---|
1909 | |
---|
1910 | if (!treeprint) |
---|
1911 | return; |
---|
1912 | putc('\n', outfile); |
---|
1913 | tipy = 1; |
---|
1914 | tipmax = 0.0; |
---|
1915 | proml_coordinates(curtree.start, 0.0, &tipy, &tipmax); |
---|
1916 | scale = 1.0 / (long)(tipmax + 1.000); |
---|
1917 | for (i = 1; i <= (tipy - down); i++) |
---|
1918 | drawline2(i, scale, curtree); |
---|
1919 | putc('\n', outfile); |
---|
1920 | } /* proml_printree */ |
---|
1921 | |
---|
1922 | |
---|
1923 | void sigma(node *p, double *sumlr, double *s1, double *s2) |
---|
1924 | { |
---|
1925 | /* compute standard deviation */ |
---|
1926 | double tt, aa, like, slope, curv; |
---|
1927 | |
---|
1928 | prot_slopecurv(p, p->v, &like, &slope, &curv); |
---|
1929 | tt = p->v; |
---|
1930 | p->v = epsilon; |
---|
1931 | p->back->v = epsilon; |
---|
1932 | aa = prot_evaluate(p, false); |
---|
1933 | p->v = tt; |
---|
1934 | p->back->v = tt; |
---|
1935 | (*sumlr) = prot_evaluate(p, false) - aa; |
---|
1936 | if (curv < -epsilon) { |
---|
1937 | (*s1) = p->v + (-slope - sqrt(slope * slope - 3.841 * curv)) / curv; |
---|
1938 | (*s2) = p->v + (-slope + sqrt(slope * slope - 3.841 * curv)) / curv; |
---|
1939 | } |
---|
1940 | else { |
---|
1941 | (*s1) = -1.0; |
---|
1942 | (*s2) = -1.0; |
---|
1943 | } |
---|
1944 | } /* sigma */ |
---|
1945 | |
---|
1946 | |
---|
1947 | void describe(node *p) |
---|
1948 | { |
---|
1949 | /* print out information for one branch */ |
---|
1950 | long i, num_sibs; |
---|
1951 | node *q, *sib_ptr; |
---|
1952 | double sumlr, sigma1, sigma2; |
---|
1953 | |
---|
1954 | if (!p->tip && !p->initialized) |
---|
1955 | prot_nuview(p); |
---|
1956 | if (!p->back->tip && !p->back->initialized) |
---|
1957 | prot_nuview(p->back); |
---|
1958 | q = p->back; |
---|
1959 | if (q->tip) { |
---|
1960 | fprintf(outfile, " "); |
---|
1961 | for (i = 0; i < nmlngth; i++) |
---|
1962 | putc(nayme[q->index-1][i], outfile); |
---|
1963 | fprintf(outfile, " "); |
---|
1964 | } else |
---|
1965 | fprintf(outfile, " %4ld ", q->index - spp); |
---|
1966 | if (p->tip) { |
---|
1967 | for (i = 0; i < nmlngth; i++) |
---|
1968 | putc(nayme[p->index-1][i], outfile); |
---|
1969 | } else |
---|
1970 | fprintf(outfile, "%4ld ", p->index - spp); |
---|
1971 | fprintf(outfile, "%15.5f", q->v); |
---|
1972 | if (p->iter) { |
---|
1973 | sigma(q, &sumlr, &sigma1, &sigma2); |
---|
1974 | if (sigma1 <= sigma2) |
---|
1975 | fprintf(outfile, " ( zero, infinity)"); |
---|
1976 | else { |
---|
1977 | fprintf(outfile, " ("); |
---|
1978 | if (sigma2 <= 0.0) |
---|
1979 | fprintf(outfile, " zero"); |
---|
1980 | else |
---|
1981 | fprintf(outfile, "%9.5f", sigma2); |
---|
1982 | fprintf(outfile, ",%12.5f", sigma1); |
---|
1983 | putc(')', outfile); |
---|
1984 | } |
---|
1985 | if (sumlr > 1.9205) |
---|
1986 | fprintf(outfile, " *"); |
---|
1987 | if (sumlr > 2.995) |
---|
1988 | putc('*', outfile); |
---|
1989 | } |
---|
1990 | putc('\n', outfile); |
---|
1991 | if (!p->tip) { |
---|
1992 | num_sibs = count_sibs(p); |
---|
1993 | sib_ptr = p; |
---|
1994 | for (i=0; i < num_sibs; i++) { |
---|
1995 | sib_ptr = sib_ptr->next; |
---|
1996 | describe(sib_ptr->back); |
---|
1997 | } |
---|
1998 | } |
---|
1999 | } /* describe */ |
---|
2000 | |
---|
2001 | |
---|
2002 | void prot_reconstr(node *p, long n) |
---|
2003 | { |
---|
2004 | /* reconstruct and print out acid at site n+1 at node p */ |
---|
2005 | long i, j, k, first, num_sibs = 0; |
---|
2006 | double f, sum, xx[20]; |
---|
2007 | node *q = NULL; |
---|
2008 | |
---|
2009 | if (p->tip) |
---|
2010 | putc(y[p->index-1][n], outfile); |
---|
2011 | else { |
---|
2012 | num_sibs = count_sibs(p); |
---|
2013 | if ((ally[n] == 0) || (location[ally[n]-1] == 0)) |
---|
2014 | putc('.', outfile); |
---|
2015 | else { |
---|
2016 | j = location[ally[n]-1] - 1; |
---|
2017 | sum = 0; |
---|
2018 | for (i = 0; i <= 19; i++) { |
---|
2019 | f = p->protx[j][mx-1][i]; |
---|
2020 | if (!p->tip) { |
---|
2021 | q = p; |
---|
2022 | for (k = 0; k < num_sibs; k++) { |
---|
2023 | q = q->next; |
---|
2024 | f *= q->protx[j][mx-1][i]; |
---|
2025 | } |
---|
2026 | } |
---|
2027 | f = sqrt(f); |
---|
2028 | xx[i] = f * freqaa[i]; |
---|
2029 | sum += xx[i]; |
---|
2030 | } |
---|
2031 | for (i = 0; i <= 19; i++) |
---|
2032 | xx[i] /= sum; |
---|
2033 | first = 0; |
---|
2034 | for (i = 0; i <= 19; i++) |
---|
2035 | if (xx[i] > xx[first]) |
---|
2036 | first = i; |
---|
2037 | if (xx[first] > 0.95) |
---|
2038 | putc(aachar[first], outfile); |
---|
2039 | else |
---|
2040 | putc(tolower(aachar[first]), outfile); |
---|
2041 | if (rctgry && rcategs > 1) |
---|
2042 | mx = mp[n][mx - 1]; |
---|
2043 | else |
---|
2044 | mx = 1; |
---|
2045 | } |
---|
2046 | } |
---|
2047 | } /* prot_reconstr */ |
---|
2048 | |
---|
2049 | |
---|
2050 | void rectrav(node *p, long m, long n) |
---|
2051 | { |
---|
2052 | /* print out segment of reconstructed sequence for one branch */ |
---|
2053 | long i; |
---|
2054 | |
---|
2055 | putc(' ', outfile); |
---|
2056 | if (p->tip) { |
---|
2057 | for (i = 0; i < nmlngth; i++) |
---|
2058 | putc(nayme[p->index-1][i], outfile); |
---|
2059 | } else |
---|
2060 | fprintf(outfile, "%4ld ", p->index - spp); |
---|
2061 | fprintf(outfile, " "); |
---|
2062 | mx = mx0; |
---|
2063 | for (i = m; i <= n; i++) { |
---|
2064 | if ((i % 10 == 0) && (i != m)) |
---|
2065 | putc(' ', outfile); |
---|
2066 | prot_reconstr(p, i); |
---|
2067 | } |
---|
2068 | putc('\n', outfile); |
---|
2069 | if (!p->tip) { |
---|
2070 | rectrav(p->next->back, m, n); |
---|
2071 | rectrav(p->next->next->back, m, n); |
---|
2072 | } |
---|
2073 | mx1 = mx; |
---|
2074 | } /* rectrav */ |
---|
2075 | |
---|
2076 | |
---|
2077 | void summarize() |
---|
2078 | { |
---|
2079 | /* print out branch length information and node numbers */ |
---|
2080 | long i, j, k, mm, num_sibs; |
---|
2081 | double mode, sum; |
---|
2082 | double like[maxcategs],nulike[maxcategs]; |
---|
2083 | double **marginal; |
---|
2084 | node *sib_ptr; |
---|
2085 | |
---|
2086 | if (!treeprint) |
---|
2087 | return; |
---|
2088 | fprintf(outfile, "\nremember: "); |
---|
2089 | if (outgropt) |
---|
2090 | fprintf(outfile, "(although rooted by outgroup) "); |
---|
2091 | fprintf(outfile, "this is an unrooted tree!\n\n"); |
---|
2092 | fprintf(outfile, "Ln Likelihood = %11.5f\n", curtree.likelihood); |
---|
2093 | fprintf(outfile, "\n Between And Length"); |
---|
2094 | if (!(usertree && lngths)) |
---|
2095 | fprintf(outfile, " Approx. Confidence Limits"); |
---|
2096 | fprintf(outfile, "\n"); |
---|
2097 | fprintf(outfile, " ------- --- ------"); |
---|
2098 | if (!(usertree && lngths)) |
---|
2099 | fprintf(outfile, " ------- ---------- ------"); |
---|
2100 | fprintf(outfile, "\n\n"); |
---|
2101 | for (i = spp; i < nonodes2; i++) { |
---|
2102 | /* So this works with arbitrary multifurcations */ |
---|
2103 | if (curtree.nodep[i]) { |
---|
2104 | num_sibs = count_sibs (curtree.nodep[i]); |
---|
2105 | sib_ptr = curtree.nodep[i]; |
---|
2106 | for (j = 0; j < num_sibs; j++) { |
---|
2107 | sib_ptr->initialized = false; |
---|
2108 | sib_ptr = sib_ptr->next; |
---|
2109 | } |
---|
2110 | } |
---|
2111 | } |
---|
2112 | |
---|
2113 | describe(curtree.start->back); |
---|
2114 | |
---|
2115 | /* So this works with arbitrary multifurcations */ |
---|
2116 | num_sibs = count_sibs(curtree.start); |
---|
2117 | sib_ptr = curtree.start; |
---|
2118 | for (i=0; i < num_sibs; i++) { |
---|
2119 | sib_ptr = sib_ptr->next; |
---|
2120 | describe(sib_ptr->back); |
---|
2121 | } |
---|
2122 | |
---|
2123 | fprintf(outfile, "\n"); |
---|
2124 | if (!(usertree && lngths)) { |
---|
2125 | fprintf(outfile, " * = significantly positive, P < 0.05\n"); |
---|
2126 | fprintf(outfile, " ** = significantly positive, P < 0.01\n\n"); |
---|
2127 | } |
---|
2128 | dummy = prot_evaluate(curtree.start, false); |
---|
2129 | if (rctgry && rcategs > 1) { |
---|
2130 | for (i = 0; i < rcategs; i++) |
---|
2131 | like[i] = 1.0; |
---|
2132 | for (i = sites - 1; i >= 0; i--) { |
---|
2133 | sum = 0.0; |
---|
2134 | for (j = 0; j < rcategs; j++) { |
---|
2135 | nulike[j] = (1.0 - lambda + lambda * probcat[j]) * like[j]; |
---|
2136 | mp[i][j] = j + 1; |
---|
2137 | for (k = 1; k <= rcategs; k++) { |
---|
2138 | if (k != j + 1) { |
---|
2139 | if (lambda * probcat[k - 1] * like[k - 1] > nulike[j]) { |
---|
2140 | nulike[j] = lambda * probcat[k - 1] * like[k - 1]; |
---|
2141 | mp[i][j] = k; |
---|
2142 | } |
---|
2143 | } |
---|
2144 | } |
---|
2145 | if ((ally[i] > 0) && (location[ally[i]-1] > 0)) |
---|
2146 | nulike[j] *= contribution[location[ally[i] - 1] - 1][j]; |
---|
2147 | sum += nulike[j]; |
---|
2148 | } |
---|
2149 | for (j = 0; j < rcategs; j++) |
---|
2150 | nulike[j] /= sum; |
---|
2151 | memcpy(like, nulike, rcategs * sizeof(double)); |
---|
2152 | } |
---|
2153 | mode = 0.0; |
---|
2154 | mx = 1; |
---|
2155 | for (i = 1; i <= rcategs; i++) { |
---|
2156 | if (probcat[i - 1] * like[i - 1] > mode) { |
---|
2157 | mx = i; |
---|
2158 | mode = probcat[i - 1] * like[i - 1]; |
---|
2159 | } |
---|
2160 | } |
---|
2161 | mx0 = mx; |
---|
2162 | fprintf(outfile, |
---|
2163 | "Combination of categories that contributes the most to the likelihood:\n\n"); |
---|
2164 | for (i = 1; i <= nmlngth + 3; i++) |
---|
2165 | putc(' ', outfile); |
---|
2166 | for (i = 1; i <= sites; i++) { |
---|
2167 | fprintf(outfile, "%ld", mx); |
---|
2168 | if (i % 10 == 0) |
---|
2169 | putc(' ', outfile); |
---|
2170 | if (i % 60 == 0 && i != sites) { |
---|
2171 | putc('\n', outfile); |
---|
2172 | for (j = 1; j <= nmlngth + 3; j++) |
---|
2173 | putc(' ', outfile); |
---|
2174 | } |
---|
2175 | mx = mp[i - 1][mx - 1]; |
---|
2176 | } |
---|
2177 | fprintf(outfile, "\n\n"); |
---|
2178 | marginal = (double **) Malloc(sites*sizeof(double *)); |
---|
2179 | for (i = 0; i < sites; i++) |
---|
2180 | marginal[i] = (double *) Malloc(rcategs*sizeof(double)); |
---|
2181 | for (i = 0; i < rcategs; i++) |
---|
2182 | like[i] = 1.0; |
---|
2183 | for (i = sites - 1; i >= 0; i--) { |
---|
2184 | sum = 0.0; |
---|
2185 | for (j = 0; j < rcategs; j++) { |
---|
2186 | nulike[j] = (1.0 - lambda + lambda * probcat[j]) * like[j]; |
---|
2187 | for (k = 1; k <= rcategs; k++) { |
---|
2188 | if (k != j + 1) |
---|
2189 | nulike[j] += lambda * probcat[k - 1] * like[k - 1]; |
---|
2190 | } |
---|
2191 | if ((ally[i] > 0) && (location[ally[i]-1] > 0)) |
---|
2192 | nulike[j] *= contribution[location[ally[i] - 1] - 1][j]; |
---|
2193 | sum += nulike[j]; |
---|
2194 | } |
---|
2195 | for (j = 0; j < rcategs; j++) { |
---|
2196 | nulike[j] /= sum; |
---|
2197 | marginal[i][j] = nulike[j]; |
---|
2198 | } |
---|
2199 | memcpy(like, nulike, rcategs * sizeof(double)); |
---|
2200 | } |
---|
2201 | for (i = 0; i < rcategs; i++) |
---|
2202 | like[i] = 1.0; |
---|
2203 | for (i = 0; i < sites; i++) { |
---|
2204 | sum = 0.0; |
---|
2205 | for (j = 0; j < rcategs; j++) { |
---|
2206 | nulike[j] = (1.0 - lambda + lambda * probcat[j]) * like[j]; |
---|
2207 | for (k = 1; k <= rcategs; k++) { |
---|
2208 | if (k != j + 1) |
---|
2209 | nulike[j] += lambda * probcat[k - 1] * like[k - 1]; |
---|
2210 | } |
---|
2211 | marginal[i][j] *= like[j] * probcat[j]; |
---|
2212 | sum += nulike[j]; |
---|
2213 | } |
---|
2214 | for (j = 0; j < rcategs; j++) |
---|
2215 | nulike[j] /= sum; |
---|
2216 | memcpy(like, nulike, rcategs * sizeof(double)); |
---|
2217 | sum = 0.0; |
---|
2218 | for (j = 0; j < rcategs; j++) |
---|
2219 | sum += marginal[i][j]; |
---|
2220 | for (j = 0; j < rcategs; j++) |
---|
2221 | marginal[i][j] /= sum; |
---|
2222 | } |
---|
2223 | fprintf(outfile, "Most probable category at each site if > 0.95"); |
---|
2224 | fprintf(outfile, " probability (\".\" otherwise)\n\n"); |
---|
2225 | for (i = 1; i <= nmlngth + 3; i++) |
---|
2226 | putc(' ', outfile); |
---|
2227 | for (i = 0; i < sites; i++) { |
---|
2228 | sum = 0.0; |
---|
2229 | for (j = 0; j < rcategs; j++) |
---|
2230 | if (marginal[i][j] > sum) { |
---|
2231 | sum = marginal[i][j]; |
---|
2232 | mm = j; |
---|
2233 | } |
---|
2234 | if (sum >= 0.95) |
---|
2235 | fprintf(outfile, "%ld", mm+1); |
---|
2236 | else |
---|
2237 | putc('.', outfile); |
---|
2238 | if ((i+1) % 60 == 0) { |
---|
2239 | if (i != 0) { |
---|
2240 | putc('\n', outfile); |
---|
2241 | for (j = 1; j <= nmlngth + 3; j++) |
---|
2242 | putc(' ', outfile); |
---|
2243 | } |
---|
2244 | } |
---|
2245 | else if ((i+1) % 10 == 0) |
---|
2246 | putc(' ', outfile); |
---|
2247 | } |
---|
2248 | putc('\n', outfile); |
---|
2249 | for (i = 0; i < sites; i++) |
---|
2250 | free(marginal[i]); |
---|
2251 | free(marginal); |
---|
2252 | } |
---|
2253 | putc('\n', outfile); |
---|
2254 | if (hypstate) { |
---|
2255 | fprintf(outfile, "Probable sequences at interior nodes:\n\n"); |
---|
2256 | fprintf(outfile, " node "); |
---|
2257 | for (i = 0; (i < 13) && (i < ((sites + (sites-1)/10 - 39) / 2)); i++) |
---|
2258 | putc(' ', outfile); |
---|
2259 | fprintf(outfile, "Reconstructed sequence (caps if > 0.95)\n\n"); |
---|
2260 | if (!rctgry || (rcategs == 1)) |
---|
2261 | mx0 = 1; |
---|
2262 | for (i = 0; i < sites; i += 60) { |
---|
2263 | k = i + 59; |
---|
2264 | if (k >= sites) |
---|
2265 | k = sites - 1; |
---|
2266 | rectrav(curtree.start, i, k); |
---|
2267 | rectrav(curtree.start->back, i, k); |
---|
2268 | putc('\n', outfile); |
---|
2269 | mx0 = mx1; |
---|
2270 | } |
---|
2271 | } |
---|
2272 | } /* summarize */ |
---|
2273 | |
---|
2274 | |
---|
2275 | void initpromlnode(node **p, node **local_grbg, node *UNUSED_q, long UNUSED_len, long nodei, |
---|
2276 | long *UNUSED_ntips, long *parens, initops whichinit, |
---|
2277 | pointarray UNUSED_treenode, pointarray nodep, Char *str, |
---|
2278 | Char *ch, FILE *fp_intree) |
---|
2279 | { |
---|
2280 | (void)UNUSED_q; |
---|
2281 | (void)UNUSED_len; |
---|
2282 | (void)UNUSED_ntips; |
---|
2283 | (void)UNUSED_treenode; |
---|
2284 | |
---|
2285 | /* initializes a node */ |
---|
2286 | boolean minusread; |
---|
2287 | double valyew, divisor; |
---|
2288 | |
---|
2289 | switch (whichinit) { |
---|
2290 | case bottom: |
---|
2291 | gnu(local_grbg, p); |
---|
2292 | (*p)->index = nodei; |
---|
2293 | (*p)->tip = false; |
---|
2294 | malloc_ppheno((*p), endsite, rcategs); |
---|
2295 | nodep[(*p)->index - 1] = (*p); |
---|
2296 | break; |
---|
2297 | case nonbottom: |
---|
2298 | gnu(local_grbg, p); |
---|
2299 | malloc_ppheno(*p, endsite, rcategs); |
---|
2300 | (*p)->index = nodei; |
---|
2301 | break; |
---|
2302 | case tip: |
---|
2303 | match_names_to_data(str, nodep, p, spp); |
---|
2304 | break; |
---|
2305 | case iter: |
---|
2306 | (*p)->initialized = false; |
---|
2307 | (*p)->v = initialv; |
---|
2308 | (*p)->iter = true; |
---|
2309 | if ((*p)->back != NULL) |
---|
2310 | (*p)->back->iter = true; |
---|
2311 | break; |
---|
2312 | case length: |
---|
2313 | processlength(&valyew, &divisor, ch, &minusread, fp_intree, parens); |
---|
2314 | (*p)->v = valyew / divisor; |
---|
2315 | (*p)->iter = false; |
---|
2316 | if ((*p)->back != NULL) { |
---|
2317 | (*p)->back->v = (*p)->v; |
---|
2318 | (*p)->back->iter = false; |
---|
2319 | } |
---|
2320 | break; |
---|
2321 | default: /* cases hslength, hsnolength, treewt, unittrwt */ |
---|
2322 | break; /* should never occur */ |
---|
2323 | } |
---|
2324 | } /* initpromlnode */ |
---|
2325 | |
---|
2326 | |
---|
2327 | void dnaml_treeout(node *p) |
---|
2328 | { |
---|
2329 | /* write out file with representation of final tree2 */ |
---|
2330 | /* Only works for bifurcations! */ |
---|
2331 | long i, n, w; |
---|
2332 | Char c; |
---|
2333 | double x; |
---|
2334 | |
---|
2335 | if (p->tip) { |
---|
2336 | n = 0; |
---|
2337 | for (i = 1; i <= nmlngth; i++) { |
---|
2338 | if (nayme[p->index-1][i - 1] != ' ') |
---|
2339 | n = i; |
---|
2340 | } |
---|
2341 | for (i = 0; i < n; i++) { |
---|
2342 | c = nayme[p->index-1][i]; |
---|
2343 | if (c == ' ') |
---|
2344 | c = '_'; |
---|
2345 | putc(c, outtree); |
---|
2346 | } |
---|
2347 | col += n; |
---|
2348 | } else { |
---|
2349 | putc('(', outtree); |
---|
2350 | col++; |
---|
2351 | dnaml_treeout(p->next->back); |
---|
2352 | putc(',', outtree); |
---|
2353 | col++; |
---|
2354 | if (col > 45) { |
---|
2355 | putc('\n', outtree); |
---|
2356 | col = 0; |
---|
2357 | } |
---|
2358 | dnaml_treeout(p->next->next->back); |
---|
2359 | if (p == curtree.start) { |
---|
2360 | putc(',', outtree); |
---|
2361 | col++; |
---|
2362 | if (col > 45) { |
---|
2363 | putc('\n', outtree); |
---|
2364 | col = 0; |
---|
2365 | } |
---|
2366 | dnaml_treeout(p->back); |
---|
2367 | } |
---|
2368 | putc(')', outtree); |
---|
2369 | col++; |
---|
2370 | } |
---|
2371 | x = p->v; |
---|
2372 | if (x > 0.0) |
---|
2373 | w = (long)(0.43429448222 * log(x)); |
---|
2374 | else if (x == 0.0) |
---|
2375 | w = 0; |
---|
2376 | else |
---|
2377 | w = (long)(0.43429448222 * log(-x)) + 1; |
---|
2378 | if (w < 0) |
---|
2379 | w = 0; |
---|
2380 | if (p == curtree.start) |
---|
2381 | fprintf(outtree, ";\n"); |
---|
2382 | else { |
---|
2383 | fprintf(outtree, ":%*.5f", (int)(w + 7), x); |
---|
2384 | col += w + 8; |
---|
2385 | } |
---|
2386 | } /* dnaml_treeout */ |
---|
2387 | |
---|
2388 | |
---|
2389 | void buildnewtip(long m, tree *tr) |
---|
2390 | { |
---|
2391 | node *p; |
---|
2392 | |
---|
2393 | p = tr->nodep[nextsp + spp - 3]; |
---|
2394 | hookup(tr->nodep[m - 1], p); |
---|
2395 | p->v = initialv; |
---|
2396 | p->back->v = initialv; |
---|
2397 | } /* buildnewtip */ |
---|
2398 | |
---|
2399 | |
---|
2400 | void buildsimpletree(tree *tr) |
---|
2401 | { |
---|
2402 | hookup(tr->nodep[enterorder[0] - 1], tr->nodep[enterorder[1] - 1]); |
---|
2403 | tr->nodep[enterorder[0] - 1]->v = 1.0; |
---|
2404 | tr->nodep[enterorder[0] - 1]->back->v = 1.0; |
---|
2405 | tr->nodep[enterorder[1] - 1]->v = 1.0; |
---|
2406 | tr->nodep[enterorder[1] - 1]->back->v = 1.0; |
---|
2407 | buildnewtip(enterorder[2], tr); |
---|
2408 | insert_(tr->nodep[enterorder[2] - 1]->back, |
---|
2409 | tr->nodep[enterorder[0] - 1], false); |
---|
2410 | } /* buildsimpletree */ |
---|
2411 | |
---|
2412 | |
---|
2413 | void free_all_protx (long local_nonodes, pointarray treenode) |
---|
2414 | { |
---|
2415 | /* used in proml */ |
---|
2416 | long i, j, k; |
---|
2417 | node *p; |
---|
2418 | |
---|
2419 | /* Zero thru spp are tips, */ |
---|
2420 | for (i = 0; i < spp; i++) { |
---|
2421 | for (j = 0; j < endsite; j++) |
---|
2422 | free(treenode[i]->protx[j]); |
---|
2423 | free(treenode[i]->protx); |
---|
2424 | } |
---|
2425 | |
---|
2426 | /* The rest are rings (i.e. triads) */ |
---|
2427 | for (i = spp; i < local_nonodes; i++) { |
---|
2428 | if (treenode[i] != NULL) { |
---|
2429 | p = treenode[i]; |
---|
2430 | for (j = 1; j <= 3; j++) { |
---|
2431 | for (k = 0; k < endsite; k++) |
---|
2432 | free(p->protx[k]); |
---|
2433 | free(p->protx); |
---|
2434 | p = p->next; |
---|
2435 | } |
---|
2436 | } |
---|
2437 | } |
---|
2438 | } /* free_all_protx */ |
---|
2439 | |
---|
2440 | |
---|
2441 | void maketree() |
---|
2442 | { |
---|
2443 | long i, j, k, l; |
---|
2444 | long num_sibs; |
---|
2445 | boolean dummy_haslengths, dummy_first, goteof; |
---|
2446 | pointarray dummy_treenode=NULL; |
---|
2447 | long nextnode; |
---|
2448 | node *root, *q, *r; |
---|
2449 | |
---|
2450 | prot_inittable(); |
---|
2451 | |
---|
2452 | if (usertree) { |
---|
2453 | openfile(&intree,INTREE,"input tree file", "r",progname,intreename); |
---|
2454 | numtrees = countsemic(&intree); |
---|
2455 | if (numtrees > 2) |
---|
2456 | initseed(&inseed, &inseed0, seed); |
---|
2457 | l0gl = (double *) Malloc(numtrees * sizeof(double)); |
---|
2458 | l0gf = (double **) Malloc(numtrees * sizeof(double *)); |
---|
2459 | for (i=0; i < numtrees; ++i) |
---|
2460 | l0gf[i] = (double *) Malloc(endsite * sizeof(double)); |
---|
2461 | if (treeprint) { |
---|
2462 | fprintf(outfile, "User-defined tree"); |
---|
2463 | if (numtrees > 1) |
---|
2464 | putc('s', outfile); |
---|
2465 | fprintf(outfile, ":\n\n"); |
---|
2466 | } |
---|
2467 | which = 1; |
---|
2468 | |
---|
2469 | /* This taken out of tree read, used to be [spp-1], but referring |
---|
2470 | to [0] produces output identical to what the pre-modified dnaml |
---|
2471 | produced. */ |
---|
2472 | |
---|
2473 | while (which <= numtrees) { |
---|
2474 | |
---|
2475 | /* These initializations required each time through the loop |
---|
2476 | since multiple trees require re-initialization */ |
---|
2477 | dummy_haslengths = true; |
---|
2478 | nextnode = 0; |
---|
2479 | dummy_first = true; |
---|
2480 | goteof = false; |
---|
2481 | |
---|
2482 | treeread(intree, &root, dummy_treenode, &goteof, &dummy_first, |
---|
2483 | curtree.nodep, &nextnode, |
---|
2484 | &dummy_haslengths, &grbg, initpromlnode); |
---|
2485 | q = root; |
---|
2486 | r = root; |
---|
2487 | while (!(q->next == root)) |
---|
2488 | q = q->next; |
---|
2489 | q->next = root->next; |
---|
2490 | root = q; |
---|
2491 | chuck(&grbg, r); |
---|
2492 | curtree.nodep[spp] = q; |
---|
2493 | if (goteof && (which <= numtrees)) { |
---|
2494 | /* if we hit the end of the file prematurely */ |
---|
2495 | printf ("\n"); |
---|
2496 | printf ("ERROR: trees missing at end of file.\n"); |
---|
2497 | printf ("\tExpected number of trees:\t\t%ld\n", numtrees); |
---|
2498 | printf ("\tNumber of trees actually in file:\t%ld.\n\n", which - 1); |
---|
2499 | exxit (-1); |
---|
2500 | } |
---|
2501 | |
---|
2502 | curtree.start = curtree.nodep[0]->back; |
---|
2503 | |
---|
2504 | treevaluate(); |
---|
2505 | if (reconsider) { |
---|
2506 | bestyet = UNDEFINED; |
---|
2507 | succeeded = true; |
---|
2508 | while (succeeded) { |
---|
2509 | succeeded = false; |
---|
2510 | rearrange(curtree.start, curtree.start->back); |
---|
2511 | } |
---|
2512 | treevaluate(); |
---|
2513 | } |
---|
2514 | proml_printree(); |
---|
2515 | summarize(); |
---|
2516 | if (trout) { |
---|
2517 | col = 0; |
---|
2518 | dnaml_treeout(curtree.start); |
---|
2519 | } |
---|
2520 | which++; |
---|
2521 | } |
---|
2522 | FClose(intree); |
---|
2523 | putc('\n', outfile); |
---|
2524 | if (!auto_ && numtrees > 1 && weightsum > 1 ) |
---|
2525 | standev2(numtrees, maxwhich, 0, endsite-1, maxlogl, |
---|
2526 | l0gl, l0gf, aliasweight, seed); |
---|
2527 | } else { |
---|
2528 | /* If there's no input user tree, */ |
---|
2529 | for (i = 1; i <= spp; i++) |
---|
2530 | enterorder[i - 1] = i; |
---|
2531 | if (jumble) |
---|
2532 | randumize(seed, enterorder); |
---|
2533 | if (progress) { |
---|
2534 | printf("\nAdding species:\n"); |
---|
2535 | writename(0, 3, enterorder); |
---|
2536 | #ifdef WIN32 |
---|
2537 | phyFillScreenColor(); |
---|
2538 | #endif |
---|
2539 | } |
---|
2540 | nextsp = 3; |
---|
2541 | polishing = false; |
---|
2542 | buildsimpletree(&curtree); |
---|
2543 | curtree.start = curtree.nodep[enterorder[0] - 1]->back; |
---|
2544 | smoothit = improve; |
---|
2545 | nextsp = 4; |
---|
2546 | while (nextsp <= spp) { |
---|
2547 | buildnewtip(enterorder[nextsp - 1], &curtree); |
---|
2548 | bestyet = UNDEFINED; |
---|
2549 | if (smoothit) |
---|
2550 | promlcopy(&curtree, &priortree, nonodes2, rcategs); |
---|
2551 | addtraverse(curtree.nodep[enterorder[nextsp - 1] - 1]->back, |
---|
2552 | curtree.start, true); |
---|
2553 | if (smoothit) |
---|
2554 | promlcopy(&bestree, &curtree, nonodes2, rcategs); |
---|
2555 | else { |
---|
2556 | insert_(curtree.nodep[enterorder[nextsp - 1] - 1]->back, qwhere, true); |
---|
2557 | smoothit = true; |
---|
2558 | for (i = 1; i<=smoothings; i++) { |
---|
2559 | smooth(curtree.start); |
---|
2560 | smooth(curtree.start->back); |
---|
2561 | } |
---|
2562 | smoothit = false; |
---|
2563 | promlcopy(&curtree, &bestree, nonodes2, rcategs); |
---|
2564 | bestyet = curtree.likelihood; |
---|
2565 | } |
---|
2566 | if (progress) { |
---|
2567 | writename(nextsp - 1, 1, enterorder); |
---|
2568 | #ifdef WIN32 |
---|
2569 | phyFillScreenColor(); |
---|
2570 | #endif |
---|
2571 | } |
---|
2572 | if (global && nextsp == spp && progress) { |
---|
2573 | printf("Doing global rearrangements\n"); |
---|
2574 | printf(" !"); |
---|
2575 | for (j = 1; j <= (spp - 3); j++) |
---|
2576 | putchar('-'); |
---|
2577 | printf("!\n"); |
---|
2578 | #ifdef WIN32 |
---|
2579 | phyFillScreenColor(); |
---|
2580 | #endif |
---|
2581 | } |
---|
2582 | succeeded = true; |
---|
2583 | while (succeeded) { |
---|
2584 | succeeded = false; |
---|
2585 | if (global && nextsp == spp && progress) { |
---|
2586 | printf(" "); |
---|
2587 | fflush(stdout); |
---|
2588 | } |
---|
2589 | rearrange(curtree.start, curtree.start->back); |
---|
2590 | if (global && nextsp == spp && progress) |
---|
2591 | putchar('\n'); |
---|
2592 | } |
---|
2593 | for (i = spp; i < nextsp + spp - 2; i++) { |
---|
2594 | curtree.nodep[i]->initialized = false; |
---|
2595 | curtree.nodep[i]->next->initialized = false; |
---|
2596 | curtree.nodep[i]->next->next->initialized = false; |
---|
2597 | } |
---|
2598 | if (!smoothit) { |
---|
2599 | smoothit = true; |
---|
2600 | for (i = 1; i<=smoothings; i++) { |
---|
2601 | smooth(curtree.start); |
---|
2602 | smooth(curtree.start->back); |
---|
2603 | } |
---|
2604 | smoothit = false; |
---|
2605 | promlcopy(&curtree, &bestree, nonodes2, rcategs); |
---|
2606 | bestyet = curtree.likelihood; |
---|
2607 | } |
---|
2608 | nextsp++; |
---|
2609 | } |
---|
2610 | if (global && progress) { |
---|
2611 | putchar('\n'); |
---|
2612 | fflush(stdout); |
---|
2613 | #ifdef WIN32 |
---|
2614 | phyFillScreenColor(); |
---|
2615 | #endif |
---|
2616 | } |
---|
2617 | if (njumble > 1) { |
---|
2618 | if (jumb == 1) |
---|
2619 | promlcopy(&bestree, &bestree2, nonodes2, rcategs); |
---|
2620 | else |
---|
2621 | if (bestree2.likelihood < bestree.likelihood) |
---|
2622 | promlcopy(&bestree, &bestree2, nonodes2, rcategs); |
---|
2623 | } |
---|
2624 | if (jumb == njumble) { |
---|
2625 | if (njumble > 1) |
---|
2626 | promlcopy(&bestree2, &curtree, nonodes2, rcategs); |
---|
2627 | curtree.start = curtree.nodep[outgrno - 1]->back; |
---|
2628 | for (i = 0; i < nonodes2; i++) { |
---|
2629 | if (i < spp) |
---|
2630 | curtree.nodep[i]->initialized = false; |
---|
2631 | else { |
---|
2632 | curtree.nodep[i]->initialized = false; |
---|
2633 | curtree.nodep[i]->next->initialized = false; |
---|
2634 | curtree.nodep[i]->next->next->initialized = false; |
---|
2635 | } |
---|
2636 | } |
---|
2637 | treevaluate(); |
---|
2638 | proml_printree(); |
---|
2639 | summarize(); |
---|
2640 | if (trout) { |
---|
2641 | col = 0; |
---|
2642 | dnaml_treeout(curtree.start); |
---|
2643 | } |
---|
2644 | } |
---|
2645 | } |
---|
2646 | if (usertree) { |
---|
2647 | free(l0gl); |
---|
2648 | for (i=0; i < numtrees; i++) |
---|
2649 | free(l0gf[i]); |
---|
2650 | free(l0gf); |
---|
2651 | } |
---|
2652 | for (num_sibs = 0; num_sibs < max_num_sibs; num_sibs++) { |
---|
2653 | for (j = 0; j < rcategs; j++) { |
---|
2654 | for (k = 0; k < categs; k++) { |
---|
2655 | for (l = 0; l < 20; l++) { |
---|
2656 | free(pmatrices[num_sibs][j][k][l]); |
---|
2657 | } |
---|
2658 | free(pmatrices[num_sibs][j][k]); |
---|
2659 | } |
---|
2660 | free(pmatrices[num_sibs][j]); |
---|
2661 | } |
---|
2662 | free(pmatrices[num_sibs]); |
---|
2663 | } |
---|
2664 | if (jumb < njumble) |
---|
2665 | return; |
---|
2666 | free(contribution); |
---|
2667 | free(mp); |
---|
2668 | for (i=0; i < endsite; i++) |
---|
2669 | free(term[i]); |
---|
2670 | free(term); |
---|
2671 | for (i=0; i < endsite; i++) |
---|
2672 | free(slopeterm[i]); |
---|
2673 | free(slopeterm); |
---|
2674 | for (i=0; i < endsite; i++) |
---|
2675 | free(curveterm[i]); |
---|
2676 | free(curveterm); |
---|
2677 | free_all_protx(nonodes2, curtree.nodep); |
---|
2678 | if (!usertree || reconsider) { |
---|
2679 | free_all_protx(nonodes2, bestree.nodep); |
---|
2680 | free_all_protx(nonodes2, priortree.nodep); |
---|
2681 | if (njumble > 1) |
---|
2682 | free_all_protx(nonodes2, bestree2.nodep); |
---|
2683 | } |
---|
2684 | if (progress) { |
---|
2685 | printf("\n\nOutput written to file \"%s\"\n\n", outfilename); |
---|
2686 | if (trout) |
---|
2687 | printf("Tree also written onto file \"%s\"\n", outtreename); |
---|
2688 | putchar('\n'); |
---|
2689 | } |
---|
2690 | } /* maketree */ |
---|
2691 | |
---|
2692 | |
---|
2693 | void clean_up() |
---|
2694 | { |
---|
2695 | /* Free and/or close stuff */ |
---|
2696 | long i; |
---|
2697 | |
---|
2698 | free (rrate); |
---|
2699 | free (probcat); |
---|
2700 | free (rate); |
---|
2701 | /* Seems to require freeing every time... */ |
---|
2702 | for (i = 0; i < spp; i++) { |
---|
2703 | free (y[i]); |
---|
2704 | } |
---|
2705 | free (y); |
---|
2706 | free (nayme); |
---|
2707 | free (enterorder); |
---|
2708 | free (category); |
---|
2709 | free (weight); |
---|
2710 | free (alias); |
---|
2711 | free (ally); |
---|
2712 | free (location); |
---|
2713 | free (aliasweight); |
---|
2714 | free (probmat); |
---|
2715 | free (eigmat); |
---|
2716 | |
---|
2717 | #if 0 /* ???? debug ???? */ |
---|
2718 | freetree2(curtree.nodep, nonodes2); |
---|
2719 | |
---|
2720 | if (! (usertree && !reconsider)) { |
---|
2721 | freetree2(bestree.nodep, nonodes2); |
---|
2722 | freetree2(priortree.nodep, nonodes2); |
---|
2723 | } |
---|
2724 | |
---|
2725 | if (! (njumble <= 1)) |
---|
2726 | freetree2(bestree2.nodep, nonodes2); |
---|
2727 | #endif |
---|
2728 | FClose(infile); |
---|
2729 | FClose(outfile); |
---|
2730 | FClose(outtree); |
---|
2731 | #ifdef MAC |
---|
2732 | fixmacfile(outfilename); |
---|
2733 | fixmacfile(outtreename); |
---|
2734 | #endif |
---|
2735 | } /* clean_up */ |
---|
2736 | |
---|
2737 | |
---|
2738 | int main(int argc, Char *argv[]) |
---|
2739 | { /* Protein Maximum Likelihood */ |
---|
2740 | #ifdef MAC |
---|
2741 | argc = 1; /* macsetup("ProML",""); */ |
---|
2742 | argv[0] = "ProML"; |
---|
2743 | #endif |
---|
2744 | init(argc,argv); |
---|
2745 | progname = argv[0]; |
---|
2746 | openfile(&infile,INFILE,"input file","r",argv[0],infilename); |
---|
2747 | openfile(&outfile,OUTFILE,"output file","w",argv[0],outfilename); |
---|
2748 | mulsets = false; |
---|
2749 | datasets = 1; |
---|
2750 | firstset = true; |
---|
2751 | ibmpc = IBMCRT; |
---|
2752 | ansi = ANSICRT; |
---|
2753 | grbg = NULL; |
---|
2754 | doinit(); |
---|
2755 | if (ctgry) |
---|
2756 | openfile(&catfile,CATFILE,"categories file","r",argv[0],catfilename); |
---|
2757 | if (weights || justwts) |
---|
2758 | openfile(&weightfile,WEIGHTFILE,"weights file","r",argv[0],weightfilename); |
---|
2759 | if (trout) |
---|
2760 | openfile(&outtree,OUTTREE,"output tree file","w",argv[0],outtreename); |
---|
2761 | for (ith = 1; ith <= datasets; ith++) { |
---|
2762 | if (datasets > 1) { |
---|
2763 | fprintf(outfile, "Data set # %ld:\n", ith); |
---|
2764 | printf("\nData set # %ld:\n", ith); |
---|
2765 | } |
---|
2766 | getinput(); |
---|
2767 | if (ith == 1) |
---|
2768 | firstset = false; |
---|
2769 | for (jumb = 1; jumb <= njumble; jumb++) { |
---|
2770 | max_num_sibs = 0; |
---|
2771 | maketree(); |
---|
2772 | } |
---|
2773 | } |
---|
2774 | |
---|
2775 | clean_up(); |
---|
2776 | printf("Done.\n\n"); |
---|
2777 | #ifdef WIN32 |
---|
2778 | phyRestoreConsoleAttributes(); |
---|
2779 | #endif |
---|
2780 | return 0; |
---|
2781 | } /* Protein Maximum Likelihood */ |
---|
2782 | |
---|