1 | #Please insert up references in the next lines (line starts with keyword UP) |
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2 | UP arb.hlp |
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3 | UP glossary.hlp |
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4 | UP pars.hlp |
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5 | |
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6 | #Please insert subtopic references (line starts with keyword SUB) |
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7 | SUB pa_add.hlp |
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8 | |
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9 | # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} |
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10 | |
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11 | #************* Title of helpfile !! and start of real helpfile ******** |
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12 | TITLE Add species without optimizing topology |
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13 | |
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14 | OCCURRENCE ARB_NT/Tree/Add Species to Existing Tree/ARB Parsimony (Quick add marked) |
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15 | ARB_PARSIMONY/Tree/Add Species to Tree/Add Marked Species |
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16 | ARB_PARSIMONY/Tree/Add Species to Tree/Add Selected Species |
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17 | ARB_PARSIMONY/Tree/Add Species to Tree/Remove & Add Marked Species |
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18 | |
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19 | DESCRIPTION All affected 'species' are positioned according to maximum |
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20 | parsimony criteria. The current tree topology will not be optimized |
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21 | after insertion. |
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22 | |
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23 | In case of 'Add Selected Species' the |
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24 | currently LINK{selected.hlp} gets |
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25 | inserted. The other functions always work on all 'marked species'. |
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26 | |
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27 | In case of 'Remove & Add Marked Species' any marked species are first |
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28 | removed from the tree and then inserted again. |
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29 | The other functions only add those marked species which are not |
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30 | already present in the tree. |
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31 | |
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32 | |
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33 | SECTION Full length vs partial sequence data |
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34 | |
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35 | This function only works correct when adding full-length sequences (FLS), |
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36 | where full-length here applies to the resulting positions after |
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37 | applying the selected alignment filter(s). |
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38 | |
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39 | Any partially missing sequence data will be considered an deletion and |
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40 | adds extra distance between full- and partial sequences. |
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41 | |
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42 | One common effect of doing so, is that partial sequences often group together |
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43 | although they obviously are not (parts of) near relatives, just because they |
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44 | do not share (enough) alignment regions. |
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45 | |
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46 | To avoid that problem |
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47 | - do not use partial sequence data (recommended) or |
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48 | - (if you really have to) declare and handle partial sequence |
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49 | data as such, by using LINK{pa_partial.hlp}. |
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50 | |
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51 | |
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52 | SECTION Tree degeneration |
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53 | |
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54 | As no topology optimization takes place here, the tree will tend to |
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55 | degenerate while you add more and more species, i.e. the tree will |
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56 | continuously diverge from an optimized topology build upon the same |
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57 | set of species. |
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58 | |
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59 | Nevertheless this function has proven useful to determine the |
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60 | phylogenetic position of new/custom sequences in an optimized tree. |
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61 | But you should always be aware that, the more often you add sequences |
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62 | this way, the worser your topology will get. |
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63 | |
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64 | Probably you may want to optimize your tree from time |
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65 | to time. See LINK{pa_optimizer.hlp} for howto. |
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66 | |
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67 | |
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68 | SECTION Insertion order |
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69 | |
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70 | Until arb-6.0.x species with more base positions were added before |
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71 | such with less bp. |
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72 | |
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73 | Starting with arb-7.0 all species are added independently, i.e. |
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74 | there is no explicit insertion order (see exception below). |
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75 | The performance has been improved dramatically, especially |
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76 | when adding many species and/or when adding into big trees. |
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77 | |
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78 | If multiple species are added to the same position in the tree, |
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79 | they form a subtree which will be optimized afterwards. |
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80 | |
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81 | Exception: |
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82 | |
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83 | If the number of added species exceeds 50% of the size of |
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84 | the tree (e.g. if adding more than 1000 species to a tree |
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85 | containing 2000 sp.), the species will be devided into |
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86 | multiple sets and then these sets will be inserted one after |
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87 | each other. |
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88 | Longer sequences will be added earlier in this case. |
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89 | |
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90 | |
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91 | NOTES The current parsimony value (see LINK{pa_value.hlp}) is |
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92 | displayed after the 'Current Par' prompt in the upper part of |
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93 | the 'ARB_PARSIMONY' window and the 'Message' window (pops up |
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94 | while calculating). |
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95 | |
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96 | EXAMPLES None |
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97 | |
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98 | WARNINGS The phylogenetic information conferred by the new sequence(s) is |
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99 | not used for global tree optimization. |
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100 | |
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101 | BUGS No bugs known |
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