source: trunk/HELP_SOURCE/source/pd_spec_param.hlp

#Please insert up references in the next lines (line starts with keyword UP)
UP      arb.hlp
UP      glossary.hlp
UP      probedesign.hlp

#Please insert subtopic references  (line starts with keyword SUB)
#SUB    bonds.hlp

# Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain}

#************* Title of helpfile !! and start of real helpfile ********
TITLE           Probe design (Expert)

OCCURRENCE      ARB_NT/Probes/Design Probes/EXPERT

DESCRIPTION     See LINK{bonds.hlp} for upper area.

                Define threshold for splitting melting domains:

                        Type a value to the 'Threshold for Splitting' subwindow.
                        This value is subtracted from the mean of all pairing
                        values of the potential probe target hybrid. If the
                        resulting value is lower than that of a particular
                        mispairing, it is assumed that the remaining base paired
                        stretches melt independently. The dissociation
                        temperature of the whole hybrid is that of the more
                        stable part of it.

NOTES           None

EXAMPLES        None

WARNINGS        The criteria used for probe target site ranking are based on
                experimental experience. However, optimum conditions have to be
                determined experimentally for the particular probe target
                hybrids.

BUGS            No bugs known