1 | // ==================================================================== // |
---|
2 | // // |
---|
3 | // File : MG_preserves.cxx // |
---|
4 | // Purpose : find candidates for alignment preservation // |
---|
5 | // // |
---|
6 | // // |
---|
7 | // Coded by Ralf Westram (coder@reallysoft.de) in July 2003 // |
---|
8 | // Copyright Department of Microbiology (Technical University Munich) // |
---|
9 | // // |
---|
10 | // Visit our web site at: http://www.arb-home.de/ // |
---|
11 | // // |
---|
12 | // // |
---|
13 | // ==================================================================== // |
---|
14 | |
---|
15 | #include "merge.hxx" |
---|
16 | #include "MG_adapt_ali.hxx" |
---|
17 | |
---|
18 | #include <aw_awar.hxx> |
---|
19 | #include <aw_root.hxx> |
---|
20 | #include <aw_msg.hxx> |
---|
21 | #include <aw_select.hxx> |
---|
22 | #include <arb_progress.h> |
---|
23 | #include <arb_global_defs.h> |
---|
24 | #include <arbdbt.h> |
---|
25 | |
---|
26 | #include <set> |
---|
27 | #include <string> |
---|
28 | |
---|
29 | using namespace std; |
---|
30 | |
---|
31 | // find species/SAIs to preserve alignment |
---|
32 | |
---|
33 | #define AWAR_REMAP_CANDIDATE AWAR_MERGE_TMP "remap_candidates" |
---|
34 | #define AWAR_REMAP_ALIGNMENT AWAR_MERGE_TMP "remap_alignment" |
---|
35 | #define AWAR_REMAP_SEL_REFERENCE AWAR_MERGE_TMP "remap_reference" |
---|
36 | |
---|
37 | struct preserve_para { |
---|
38 | AW_selection_list *alignmentList; |
---|
39 | AW_selection_list *refCandidatesList; // reference candidates |
---|
40 | AW_selection_list *usedRefsList; |
---|
41 | }; |
---|
42 | |
---|
43 | static void get_global_alignments(ConstStrArray& ali_names) { |
---|
44 | // get all alignment names available in both databases |
---|
45 | GBT_get_alignment_names(ali_names, GLOBAL_gb_src); |
---|
46 | GBDATA *gb_presets = GBT_get_presets(GLOBAL_gb_dst); |
---|
47 | |
---|
48 | int i; |
---|
49 | for (i = 0; ali_names[i]; ++i) { |
---|
50 | GBDATA *gb_ali_name = GB_find_string(gb_presets, "alignment_name", ali_names[i], GB_IGNORE_CASE, SEARCH_GRANDCHILD); |
---|
51 | if (!gb_ali_name) ali_names.remove(i--); |
---|
52 | } |
---|
53 | } |
---|
54 | |
---|
55 | static void init_alignments(preserve_para *para) { |
---|
56 | // initialize the alignment selection list |
---|
57 | ConstStrArray ali_names; |
---|
58 | get_global_alignments(ali_names); |
---|
59 | para->alignmentList->init_from_array(ali_names, "All", "All"); |
---|
60 | } |
---|
61 | |
---|
62 | static void clear_candidates(preserve_para *para) { |
---|
63 | // clear the candidate list |
---|
64 | AW_selection_list *candList = para->refCandidatesList; |
---|
65 | |
---|
66 | candList->clear(); |
---|
67 | candList->insert_default(DISPLAY_NONE, NO_ALI_SELECTED); |
---|
68 | candList->update(); |
---|
69 | } |
---|
70 | |
---|
71 | static long count_bases(const char *data, long len) { |
---|
72 | long count = 0; |
---|
73 | for (long i = 0; i<len; ++i) { // LOOP_VECTORIZED =0[<5] =8[<7] =2 (failed before 5.0; 8 loops reported with 5.x + 6.x; 2 loops since 7.x) |
---|
74 | if (!GAP::is_std_gap(data[i])) { |
---|
75 | ++count; |
---|
76 | } |
---|
77 | } |
---|
78 | return count; |
---|
79 | } |
---|
80 | |
---|
81 | // count real bases |
---|
82 | // (gb_data should point to ali_xxx/data) |
---|
83 | static long count_bases(GBDATA *gb_data) { |
---|
84 | long count = 0; |
---|
85 | switch (GB_read_type(gb_data)) { |
---|
86 | case GB_STRING: |
---|
87 | count = count_bases(GB_read_char_pntr(gb_data), GB_read_count(gb_data)); |
---|
88 | break; |
---|
89 | case GB_BITS: { |
---|
90 | char *bitstring = GB_read_as_string(gb_data); |
---|
91 | long len = strlen(bitstring); |
---|
92 | count = count_bases(bitstring, len); |
---|
93 | free(bitstring); |
---|
94 | break; |
---|
95 | } |
---|
96 | default: |
---|
97 | GB_export_errorf("Data type %s is not supported", GB_get_type_name(gb_data)); |
---|
98 | break; |
---|
99 | } |
---|
100 | return count; |
---|
101 | } |
---|
102 | |
---|
103 | // ------------------------- |
---|
104 | // class Candidate |
---|
105 | |
---|
106 | class Candidate { |
---|
107 | string name; // species/SAI name |
---|
108 | double score; |
---|
109 | int found_alignments; // counts alignments with data in both databases |
---|
110 | long base_count_diff; |
---|
111 | |
---|
112 | public: |
---|
113 | Candidate(bool is_species, const char *name_, GBDATA *gb_src, GBDATA *gb_dst, const CharPtrArray& ali_names) |
---|
114 | : name(is_species ? name_ : string("SAI:")+name_) |
---|
115 | { |
---|
116 | found_alignments = 0; |
---|
117 | score = 0.0; |
---|
118 | base_count_diff = 0; |
---|
119 | bool valid = true; |
---|
120 | |
---|
121 | mg_assert(!GB_have_error()); |
---|
122 | |
---|
123 | for (int i = 0; valid && ali_names[i]; ++i) { |
---|
124 | if (GBDATA *gb_src_data = GBT_find_sequence(gb_src, ali_names[i])) { |
---|
125 | if (GBDATA *gb_dst_data = GBT_find_sequence(gb_dst, ali_names[i])) { |
---|
126 | ++found_alignments; |
---|
127 | |
---|
128 | long src_bases = count_bases(gb_src_data); |
---|
129 | long dst_bases = count_bases(gb_dst_data); |
---|
130 | base_count_diff = labs(src_bases-dst_bases); |
---|
131 | |
---|
132 | if (GB_have_error()) valid = false; |
---|
133 | |
---|
134 | double factor; |
---|
135 | if (base_count_diff>5) factor = 0.2; |
---|
136 | else factor = (6-base_count_diff)*(1.0/6); |
---|
137 | |
---|
138 | long min_bases = src_bases<dst_bases ? src_bases : dst_bases; |
---|
139 | score += min_bases*factor; |
---|
140 | } |
---|
141 | } |
---|
142 | } |
---|
143 | |
---|
144 | if (!valid) found_alignments = 0; |
---|
145 | } |
---|
146 | ~Candidate() {} |
---|
147 | |
---|
148 | int has_alignments() const { return found_alignments; } |
---|
149 | double get_score() const { return score; } |
---|
150 | |
---|
151 | const char *get_name() const { return name.c_str(); } |
---|
152 | const char *get_entry() const { |
---|
153 | return GBS_global_string("%24s %i %li %f", get_name(), found_alignments, base_count_diff, score); |
---|
154 | } |
---|
155 | |
---|
156 | bool operator < (const Candidate& other) const { // sort highest score first |
---|
157 | int ali_diff = found_alignments-other.found_alignments; |
---|
158 | if (ali_diff) return ali_diff>0; |
---|
159 | return score > other.score; |
---|
160 | } |
---|
161 | }; |
---|
162 | |
---|
163 | // use SmartPtr to memory-manage Candidate's |
---|
164 | static bool operator < (const SmartPtr<Candidate>& c1, const SmartPtr<Candidate>& c2) { |
---|
165 | return c1->operator<(*c2); |
---|
166 | } |
---|
167 | typedef set< SmartPtr<Candidate> > Candidates; |
---|
168 | |
---|
169 | static void find_species_candidates(Candidates& candidates, const CharPtrArray& ali_names) { |
---|
170 | // collect names of all species in source database |
---|
171 | GB_HASH *src_species = GBT_create_species_hash(GLOBAL_gb_src); |
---|
172 | long src_count = GBS_hash_elements(src_species); |
---|
173 | arb_progress progress("Examining species", src_count); |
---|
174 | bool aborted = false; |
---|
175 | |
---|
176 | // find existing species in destination database |
---|
177 | for (GBDATA *gb_dst_species = GBT_first_species(GLOBAL_gb_dst); |
---|
178 | gb_dst_species && !aborted; |
---|
179 | gb_dst_species = GBT_next_species(gb_dst_species)) |
---|
180 | { |
---|
181 | const char *dst_name = GBT_get_name_or_description(gb_dst_species); |
---|
182 | |
---|
183 | if (GBDATA *gb_src_species = (GBDATA*)GBS_read_hash(src_species, dst_name)) { |
---|
184 | Candidate *cand = new Candidate(true, dst_name, gb_src_species, gb_dst_species, ali_names); |
---|
185 | |
---|
186 | if (cand->has_alignments() && cand->get_score()>0.0) { |
---|
187 | candidates.insert(cand); |
---|
188 | } |
---|
189 | else { |
---|
190 | if (GB_have_error()) { |
---|
191 | aw_message(GBS_global_string("Invalid adaption candidate '%s' (%s)", dst_name, GB_await_error())); |
---|
192 | } |
---|
193 | delete cand; |
---|
194 | } |
---|
195 | |
---|
196 | progress.inc(); |
---|
197 | aborted = progress.aborted(); |
---|
198 | } |
---|
199 | } |
---|
200 | |
---|
201 | GBS_free_hash(src_species); |
---|
202 | progress.done(); |
---|
203 | } |
---|
204 | |
---|
205 | static void find_SAI_candidates(Candidates& candidates, const CharPtrArray& ali_names) { |
---|
206 | // add all candidate SAIs to 'candidates' |
---|
207 | GB_HASH *src_SAIs = GBT_create_SAI_hash(GLOBAL_gb_src); |
---|
208 | long src_count = GBS_hash_elements(src_SAIs); |
---|
209 | arb_progress progress("Examining SAIs", src_count); |
---|
210 | |
---|
211 | // find existing SAIs in destination database |
---|
212 | for (GBDATA *gb_dst_SAI = GBT_first_SAI(GLOBAL_gb_dst); |
---|
213 | gb_dst_SAI; |
---|
214 | gb_dst_SAI = GBT_next_SAI(gb_dst_SAI)) |
---|
215 | { |
---|
216 | const char *dst_name = GBT_get_name_or_description(gb_dst_SAI); |
---|
217 | |
---|
218 | if (GBDATA *gb_src_SAI = (GBDATA*)GBS_read_hash(src_SAIs, dst_name)) { |
---|
219 | Candidate *cand = new Candidate(false, dst_name, gb_src_SAI, gb_dst_SAI, ali_names); |
---|
220 | |
---|
221 | if (cand->has_alignments() && cand->get_score()>0.0) { |
---|
222 | candidates.insert(cand); |
---|
223 | } |
---|
224 | else { |
---|
225 | if (GB_have_error()) { |
---|
226 | aw_message(GBS_global_string("Invalid adaption candidate 'SAI:%s' (%s)", dst_name, GB_await_error())); |
---|
227 | } |
---|
228 | delete cand; |
---|
229 | } |
---|
230 | |
---|
231 | progress.inc(); |
---|
232 | } |
---|
233 | } |
---|
234 | |
---|
235 | GBS_free_hash(src_SAIs); |
---|
236 | } |
---|
237 | |
---|
238 | static void calculate_preserves_cb(AW_window *aww, preserve_para *para) { |
---|
239 | // FIND button (rebuild candidates list) |
---|
240 | |
---|
241 | GB_transaction ta_src(GLOBAL_gb_src); |
---|
242 | GB_transaction ta_dst(GLOBAL_gb_dst); |
---|
243 | |
---|
244 | clear_candidates(para); |
---|
245 | |
---|
246 | const char *ali = aww->get_root()->awar(AWAR_REMAP_ALIGNMENT)->read_char_pntr(); |
---|
247 | Candidates candidates; |
---|
248 | |
---|
249 | arb_progress("Searching candidates"); |
---|
250 | |
---|
251 | // add candidates |
---|
252 | { |
---|
253 | ConstStrArray ali_names; |
---|
254 | if (0 == strcmp(ali, "All")) { |
---|
255 | get_global_alignments(ali_names); |
---|
256 | } |
---|
257 | else { |
---|
258 | ali_names.put(ali); |
---|
259 | } |
---|
260 | find_SAI_candidates(candidates, ali_names); |
---|
261 | find_species_candidates(candidates, ali_names); |
---|
262 | } |
---|
263 | |
---|
264 | int count = 0; |
---|
265 | Candidates::iterator e = candidates.end(); |
---|
266 | AW_selection_list *refCandList = para->refCandidatesList; |
---|
267 | |
---|
268 | for (Candidates::iterator i = candidates.begin(); |
---|
269 | i != e && count<5000; |
---|
270 | ++i, ++count) |
---|
271 | { |
---|
272 | string name = (*i)->get_name(); |
---|
273 | string shown = (*i)->get_entry(); |
---|
274 | |
---|
275 | refCandList->insert(shown.c_str(), name.c_str()); |
---|
276 | } |
---|
277 | |
---|
278 | refCandList->update(); |
---|
279 | } |
---|
280 | |
---|
281 | |
---|
282 | |
---|
283 | static void read_references(ConstStrArray& refs, AW_root *aw_root) { |
---|
284 | char *ref_string = aw_root->awar(AWAR_REMAP_SPECIES_LIST)->read_string(); |
---|
285 | GBT_splitNdestroy_string(refs, ref_string, " \n,;", SPLIT_DROPEMPTY); |
---|
286 | } |
---|
287 | static void write_references(AW_root *aw_root, const CharPtrArray& ref_array) { |
---|
288 | char *ref_string = GBT_join_strings(ref_array, '\n'); |
---|
289 | aw_root->awar(AWAR_REMAP_SPECIES_LIST)->write_string(ref_string); |
---|
290 | aw_root->awar(AWAR_REMAP_ENABLE)->write_int(ref_string[0] != 0); |
---|
291 | free(ref_string); |
---|
292 | } |
---|
293 | static void select_reference(AW_root *aw_root, const char *ref_to_select) { |
---|
294 | aw_root->awar(AWAR_REMAP_SEL_REFERENCE)->write_string(ref_to_select); |
---|
295 | } |
---|
296 | static char *get_selected_reference(AW_root *aw_root) { |
---|
297 | return aw_root->awar(AWAR_REMAP_SEL_REFERENCE)->read_string(); |
---|
298 | } |
---|
299 | |
---|
300 | static void refresh_reference_list_cb(AW_root *aw_root, preserve_para *para) { |
---|
301 | ConstStrArray refs; |
---|
302 | read_references(refs, aw_root); |
---|
303 | para->usedRefsList->init_from_array(refs, "", ""); |
---|
304 | } |
---|
305 | |
---|
306 | static void add_selected_cb(AW_window *aww, preserve_para *para) { |
---|
307 | // ADD button (add currently selected candidate to references) |
---|
308 | AW_root *aw_root = aww->get_root(); |
---|
309 | ConstStrArray refs; |
---|
310 | read_references(refs, aw_root); |
---|
311 | |
---|
312 | char *candidate = aw_root->awar(AWAR_REMAP_CANDIDATE)->read_string(); |
---|
313 | char *selected = get_selected_reference(aw_root); |
---|
314 | int cand_index = refs.index_of(candidate); |
---|
315 | int sel_index = refs.index_of(selected); |
---|
316 | |
---|
317 | if (cand_index == -1) refs.put_before(sel_index+1, candidate); |
---|
318 | else refs.move(cand_index, sel_index); |
---|
319 | |
---|
320 | write_references(aw_root, refs); |
---|
321 | select_reference(aw_root, candidate); |
---|
322 | |
---|
323 | free(selected); |
---|
324 | free(candidate); |
---|
325 | |
---|
326 | para->refCandidatesList->move_selection(1); |
---|
327 | } |
---|
328 | |
---|
329 | static void clear_references_cb(AW_window *aww) { |
---|
330 | // CLEAR button (clear references) |
---|
331 | aww->get_root()->awar(AWAR_REMAP_SPECIES_LIST)->write_string(""); |
---|
332 | } |
---|
333 | |
---|
334 | static void del_reference_cb(AW_window *aww) { |
---|
335 | AW_root *aw_root = aww->get_root(); |
---|
336 | ConstStrArray refs; |
---|
337 | read_references(refs, aw_root); |
---|
338 | |
---|
339 | char *selected = get_selected_reference(aw_root); |
---|
340 | int sel_index = refs.index_of(selected); |
---|
341 | |
---|
342 | if (sel_index >= 0) { |
---|
343 | select_reference(aw_root, refs[sel_index+1]); |
---|
344 | refs.safe_remove(sel_index); |
---|
345 | write_references(aw_root, refs); |
---|
346 | } |
---|
347 | |
---|
348 | free(selected); |
---|
349 | } |
---|
350 | |
---|
351 | static void lower_reference_cb(AW_window *aww) { |
---|
352 | AW_root *aw_root = aww->get_root(); |
---|
353 | ConstStrArray refs; |
---|
354 | read_references(refs, aw_root); |
---|
355 | |
---|
356 | char *selected = get_selected_reference(aw_root); |
---|
357 | int sel_index = refs.index_of(selected); |
---|
358 | |
---|
359 | if (sel_index >= 0) { |
---|
360 | refs.move(sel_index, sel_index+1); |
---|
361 | write_references(aw_root, refs); |
---|
362 | } |
---|
363 | |
---|
364 | free(selected); |
---|
365 | } |
---|
366 | static void raise_reference_cb(AW_window *aww) { |
---|
367 | AW_root *aw_root = aww->get_root(); |
---|
368 | ConstStrArray refs; |
---|
369 | read_references(refs, aw_root); |
---|
370 | |
---|
371 | char *selected = get_selected_reference(aw_root); |
---|
372 | int sel_index = refs.index_of(selected); |
---|
373 | |
---|
374 | if (sel_index > 0) { |
---|
375 | refs.move(sel_index, sel_index-1); |
---|
376 | write_references(aw_root, refs); |
---|
377 | } |
---|
378 | |
---|
379 | free(selected); |
---|
380 | } |
---|
381 | |
---|
382 | static void test_references_cb(AW_window *aww) { |
---|
383 | char *reference_species_names = aww->get_root()->awar(AWAR_REMAP_SPECIES_LIST)->read_string(); |
---|
384 | GB_transaction tm(GLOBAL_gb_src); |
---|
385 | GB_transaction td(GLOBAL_gb_dst); |
---|
386 | |
---|
387 | MG_remaps test_mapping(GLOBAL_gb_src, GLOBAL_gb_dst, true, reference_species_names); // will raise aw_message's in case of problems |
---|
388 | |
---|
389 | free(reference_species_names); |
---|
390 | } |
---|
391 | |
---|
392 | static void init_preserve_awars(AW_root *aw_root) { |
---|
393 | aw_root->awar_string(AWAR_REMAP_ALIGNMENT, "", GLOBAL_gb_dst); |
---|
394 | aw_root->awar_string(AWAR_REMAP_CANDIDATE, "", GLOBAL_gb_dst); |
---|
395 | aw_root->awar_string(AWAR_REMAP_SEL_REFERENCE, "", GLOBAL_gb_dst); |
---|
396 | } |
---|
397 | |
---|
398 | AW_window *MG_create_preserves_selection_window(AW_root *aw_root) { |
---|
399 | // SELECT PRESERVES window |
---|
400 | init_preserve_awars(aw_root); |
---|
401 | |
---|
402 | AW_window_simple *aws = new AW_window_simple; |
---|
403 | |
---|
404 | aws->init(aw_root, "SELECT_PRESERVES", "Select adaption candidates"); |
---|
405 | aws->load_xfig("merge/preserves.fig"); |
---|
406 | |
---|
407 | aws->at("close"); |
---|
408 | aws->callback(AW_POPDOWN); |
---|
409 | aws->create_button("CLOSE", "CLOSE", "C"); |
---|
410 | |
---|
411 | aws->at("help"); |
---|
412 | aws->callback(makeHelpCallback("mg_preserve.hlp")); |
---|
413 | aws->create_button("HELP", "HELP", "H"); |
---|
414 | |
---|
415 | // ---------- |
---|
416 | |
---|
417 | preserve_para *para = new preserve_para; // do not free (is passed to callback) |
---|
418 | |
---|
419 | aws->at("ali"); |
---|
420 | para->alignmentList = aws->create_selection_list(AWAR_REMAP_ALIGNMENT, 10, 30); |
---|
421 | |
---|
422 | // ---------- |
---|
423 | |
---|
424 | aws->at("adapt"); |
---|
425 | aws->label("Adapt alignments"); |
---|
426 | aws->create_toggle(AWAR_REMAP_ENABLE); |
---|
427 | |
---|
428 | aws->at("reference"); |
---|
429 | para->usedRefsList = aws->create_selection_list(AWAR_REMAP_SEL_REFERENCE, 10, 30); |
---|
430 | |
---|
431 | aws->button_length(8); |
---|
432 | |
---|
433 | aws->at("clear"); |
---|
434 | aws->callback(clear_references_cb); |
---|
435 | aws->create_button("CLEAR", "Clear", "C"); |
---|
436 | |
---|
437 | aws->at("del"); |
---|
438 | aws->callback(del_reference_cb); |
---|
439 | aws->create_button("DEL", "Del", "L"); |
---|
440 | |
---|
441 | aws->at("up"); |
---|
442 | aws->callback(raise_reference_cb); |
---|
443 | aws->create_button("UP", "Up", "U"); |
---|
444 | |
---|
445 | aws->at("down"); |
---|
446 | aws->callback(lower_reference_cb); |
---|
447 | aws->create_button("DOWN", "Down", "D"); |
---|
448 | |
---|
449 | aws->at("test"); |
---|
450 | aws->callback(test_references_cb); |
---|
451 | aws->create_button("TEST", "Test", "T"); |
---|
452 | |
---|
453 | // ---------- |
---|
454 | |
---|
455 | aws->at("find"); |
---|
456 | aws->callback(makeWindowCallback(calculate_preserves_cb, para)); |
---|
457 | aws->create_autosize_button("FIND", "Find candidates", "F", 1); |
---|
458 | |
---|
459 | aws->at("add"); |
---|
460 | aws->callback(makeWindowCallback(add_selected_cb, para)); |
---|
461 | aws->create_button("ADD", "Add", "A"); |
---|
462 | |
---|
463 | aws->at("candidate"); |
---|
464 | para->refCandidatesList = aws->create_selection_list(AWAR_REMAP_CANDIDATE, 10, 30); |
---|
465 | |
---|
466 | { |
---|
467 | GB_transaction ta_src(GLOBAL_gb_src); |
---|
468 | GB_transaction ta_dst(GLOBAL_gb_dst); |
---|
469 | |
---|
470 | init_alignments(para); |
---|
471 | clear_candidates(para); |
---|
472 | } |
---|
473 | |
---|
474 | { |
---|
475 | AW_awar *awar_list = aw_root->awar(AWAR_REMAP_SPECIES_LIST); |
---|
476 | awar_list->add_callback(makeRootCallback(refresh_reference_list_cb, para)); |
---|
477 | awar_list->touch(); // trigger callback |
---|
478 | } |
---|
479 | |
---|
480 | return aws; |
---|
481 | } |
---|
482 | |
---|
483 | |
---|
484 | |
---|
485 | |
---|