source: tags/ms_r16q4/HELP_SOURCE/oldhelp/pa_partial.hlp

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1#Please insert up references in the next lines (line starts with keyword UP)
2UP      arb.hlp
3UP      glossary.hlp
4
5#Please insert subtopic references  (line starts with keyword SUB)
6SUB     partial_sequences.hlp
7SUB     pa_quick.hlp
8SUB     pa_add.hlp
9
10# Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain}
11
12#************* Title of helpfile !! and start of real helpfile ********
13TITLE           Add marked partial species
14
15OCCURRENCE      ARB_PARSIMONY/Tree/Add Species to Tree/Add Marked Partial Species
16
17DESCRIPTION     Use this to add sequences with partial sequence data into an existing tree.
18                The current tree topology will not be optimized after insertion.
19
20                The branchlengths of the added partial sequences represent the
21                number of (weighted) mutations against the full sequence.
22
23                Only the overlapping part of both sequences is taken into account and
24                the distance to the FLS will be weighted by the length of the overlap.
25
26                Different partial sequences will never attract each other, simply because
27                they are not considered as 'possible neighbours' (see also section about
28                partials in LINK{pa_quick.hlp}).
29
30                As for non-partial sequences, when using a filter only the unfiltered
31                positions will be taken into account to calculate the number of mutations.
32
33                The insertion order has no effect on the resulting placement of the
34                partial sequences. For each sequence, the best matching
35                full-length sequence (FLS) is searched and afterwards all partial
36                sequences are placed next to their detected FLS.
37
38                Often partial sequences have equal distances to more than one FLS. This happens
39                whenever two FLS only differ in alignment regions, where the partial sequence has
40                no data. In that case a warning is printed ("Insertion of '<name>' is ambiguous")
41                and one of the ambiguous insertion possibilities is chosen.
42                This is more likely to happen for low amounts of sequence data (i.e. very short sequences).
43                Consider to remove these species from the tree, as their placement might be
44                meaningless or misleading.
45
46NOTES           Adding species using this function marks them as "partial sequence" if
47                they have no 'ARB_partial' entry yet.
48                If they were already marked as FLS, the insertion is aborted with an error.
49
50                All species already in tree, when calling this function, will be marked
51                as FLS, if they have no 'ARB_partial' entry yet.
52
53                Species with partial sequences have the field "ARB_partial" set to 1, species
54                with FLS have the field "ARB_partial" set to 0.
55
56EXAMPLES        None
57
58WARNINGS        Calculating branch length after adding partial sequences
59                will lead to wrong lengths.
60
61BUGS            No bugs known
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